Multiple ovulation and embryo transfer is one of the reproductivetechnologies which is important to increase animal production. In this case report, four cows were selected as donors while elevencows were selected as recipients. Both donors and recipients had undergone the same procedures and steps for multiple ovulation and embryo transfer (MOET), such as cow preparation, synchronisation and where only donor cows follow the superovulation protocol. Cows were artificially inseminated and the embryos were flushed and graded. Out of four selected cows for donor, one of them was pregnant and only two cows actuallyproduced the embryos. The recovery rate for the embryo collection was 70.1%, and from 13 embryos (including unfertilised ova), 84.6% of the embryos was classified as good quality and suitable for embryo transfer. The overall results showed that out of eight recipients, five cows were detected pregnant, a 62.5% pregnancy rate. The aim of this report is to describe the procedures aswell as the factors that affect the successful of the MOET programme.

Reciprocal embryo transfers were made between scrapie-inoculated and scrapie-free sheep (Cheviot and Suffolk breeds) to measure scrapie transmission via the embryo (using offspring from embryos of scrapie-inoculated donors and scrapie-free recipients) and via the uterus (using offspring from embryos of scrapie-free donors and scrapie-inoculated recipients taken by cesarean section). Two control groups of offspring, 1 from scrapie-free parents (negative) and 1 from scrapie-inoculated parents (positive), also were included. All sheep were observed for clinical signs of scrapie until death or for a minimum of 60 months. Final diagnosis was made on the basis of histopathologic findings or results of mouse inoculation and/or proteinase-K-resistant protein analysis. Thirty to 61% of the scrapie-inoculated donor/recipient sheep within groups developed scrapie within 8 to 44 months after inoculation. None of the scrapie-free donor/recipients, including those gestating embryos from scrapie-inoculated donors, developed scrapie. Also, none of the offspring observed to larger than or equal to 24 months of age from reciprocal cross, via embryo (0/67), or via the uterus (0/25), or from the negative-control group (0/33) developed scrapie. Fifty-six of the offspring via embryo, 19 of these via the uterus, and 31 negative controls survived to larger than or equal to 60 months of age. Of the 21 sheep in the positive-control group, 2 (9.5%) developed scrapie, 1 at 31 months of age and 1 at 42 months of age. In the Cheviot offspring, the percentage of sheep carrying the short incubation allele ranged from 24 to 44% and the percentage in the Suffolk offspring ranged from 61 to 83%. These proportions indicate high degree of susceptibility to the disease. Results indicate that under the conditions of these experiments, scrapie was not transmitted to the offspring via the embryo or the uterus.

The role of porcine parvovirus (PPV) in inducing reproductive failure in swine has been extensively documented. However, information is not available as to the risk of ppv transmission by embryo transfer. Using the polymerase chain reaction (PCR) technique, PPV-specific DNA was detected in association with 4-day-old porcine embryos incubated in vitro in the presence of NADL-8 strain of PPV, despite attempts to rid the embryos of virus by either washing or treatment with pronase or trypsin. The presence of PPV in embryos collected from acutely infected swine was not detected by PCR, although PPV DNA was detected in the proximal portion of the reproductive tract during the early stages of infection. Viral-specific nucleic acid was not detected in embryos transferred from infected donors to seronegative recipients and retrieved and assayed on the 15th and 32nd days of gestation. Results of the use of PCR to detect PPV associated with swine female reproductive tract and embryos ascribe minimal risk to the transmission of PPV to seronegative recipients through embryo transfer.

A superovulatory and surgical protocol was developed for recovery of bovine zygotes. Holstein cows and heifers were given follicle-stimulating hormone and cloprostenol to induce superovulation. Surgical cannulation and lavage of the uterine tube was performed 40 to 48 hours after the start of standing estrus. In general, cows had more corpora hemorrhagica than did heifers, but a higher percentage (P < 0.05) of ova recovered from cows were infertile. Several heifers were subjected to the procedure twice, and embryo recovery rates were equivalent both times.

Indonesia has significant potential in the beef and dairy cattle sectors but faces challenges in improving the genetic quality of livestock. Embryo Transfer (ET) is a biotechnological approach that can enhance genetic quality and livestock productivity. This study aims to examine and analyze the factors affecting the success rate of pregnancies in FH dairy cattle as recipients of embryo transfer at Balai Embrio Ternak (BET) Cipelang. Secondary data from BET Cipelang (2017-2023) were used, including variables such as embryo quality, transfer methods, recipient age, estrus synchronization methods, and embryo donor breeds. The results showed that good embryo quality (24.55%) and fresh transfer methods (35.19%) significantly increased pregnancy success (p 0.05). Synchronization methods using PGF2 and a combination of Progesterone + PGF2 increased the chances of pregnancy by 3.60 and 3.89 times, respectively, compared to natural estrus. Although not statistically significant, recipient age groups of 3-5 years and 6-9 years had higher pregnancy success rates compared to the 10-12 years age group. The donor breed of embryos did not show a significant effect on pregnancy rates. Therefore, this study concludes that good embryo quality and fresh transfer methods significantly increase the pregnancy success rate in FH dairy cattle at BET Cipelang. The factors influencing pregnancy rates include embryo quality, transfer methods, recipient age, estrus synchronization methods, and donor breed, with embryo quality and fresh transfer methods having the greatest impact. To improve pregnancy rates, it is recommended to select high-quality embryos, use fresh transfer methods, and implement effective estrus synchronization protocols, such as PGF2 injection or a combination of Progesterone + PGF2. Further research involving various recipient cattle breeds and genetic and environmental factors is also advised.