BACKGROUND: Staphylococcus aureus is one of the most important pathogenic bacteria for human that is easily transferred during slaughtering, processing, packaging, storage and handling of meat and meat products as a result of poor hygienic principles, and causes staphylococcal food poisoning. Objectives: The objective of this study was to evaluate the contamination of raw and cooked ground beef in retail shops of Mazandaran to S. aureus and also detection of enterotoxin-producing genes in the isolates. Methods: One-hundred fifty ground beef samples (95 raw and 65 cooked) were collected randomly from retail shops, 21 May-21 July 2017. S. aureus was counted via culturing on Baird Parker Agar medium. Detection of enterotoxins A-E and G, H, I and J producing genes was conducted applying real-time PCR technique. Results: 68% of samples showed S. aureus contamination. The average count in raw and cooked ground beef samples was 3.1×105 cfu/g and 5.7×103 cfu/g, respectively. From 92 S. aureus isolates, 23 isolates (25%) were carrying enterotoxin coding genes; amongst them 15 isolates (65.2%) were carrying just a single gene and the rest more than one gene. Two isolates carrying SEA+ SEC, two isolates SEA+SEE, one isolate SEA+SEG, one isolate SEC+SEI, one isolate SEA+SEC+SEG and one isolate SEE+SEG. Conclusions: These results show that enterotoxigenic S. aureus strains are present on considerable numbers in retail ground meat in Mazandaran.

Introduction: The toxinotyping and antimicrobial susceptibility of Clostridium perfringens strains isolated from processed chicken meat were determined. Material and Methods: Two hundred processed chicken meat samples from luncheon meats, nuggets, burgers, and sausages were screened for Clostridium perfringens by multiplex PCR assay for the presence of alpha (cpa), beta (cpb), epsilon (etx), iota (ia), and enterotoxin toxin (cpe) genes. The C. perfringens isolates were examined in vitro against eight antibiotics (streptomycin, amoxicillin, ampicillin, ciprofloxacin, lincomycin, cefotaxime, rifampicin, and trimethoprim-sulfamethoxazole) Results: An overall of 32 C. perfringens strains (16%) were isolated from 200 processed chicken meat samples tested. The prevalence of C. perfringens was significantly dependent on the type of toxin genes detected (P = 0.0), being the highest in sausages (32%), followed by luncheon meats (24%), burgers (6%), and nuggets (2%). C. perfringens type A was the most frequently present toxinotype (24/32; 75%), followed by type D (21.9 %) and type E (3.1%). Of the 32 C. perfringens strains tested, only 9 (28%) were enterotoxin gene carriers, with most representing type A (n = 6). C. perfringens strains differed in their resistance/susceptibility to commonly used antibiotics. Most of the strains tested were sensitive to ampicillin (97%) and amoxicillin (94%), with 100% of the strains being resistant to streptomycin and lincomycin. It is noteworthy that the nine isolates with enterotoxigenic potential had a higher resistance than the non-enterotoxigenic ones. Conclusion: The considerably high C. perfringens isolation rates from processed chicken meat samples and resistance to some of the commonly used antibiotics indicate a potential public health risk. Recent information about the isolation of enterotoxigenic C. perfringens type E from chicken sausage has been reported.

Introduction: The toxinotyping and antimicrobial susceptibility of Clostridium perfringens strains isolated from processed chicken meat were determined.

This study was conducted to record the potential risk of some pathogens in table eggs in Beni-Suef city, Egypt. A total of 100 table eggs samples (Farm and Baladi eggs) were randomly collected from poultry farms, markets, supermarkets and groceries in Beni-Suef city, 50 farm eggs samples (each of 3 eggs) and 50 baladi eggs samples (each of 5 eggs) were examined for the presence of coliforms, E.coli, Salmonella, coagulase positive Staphylococcus aureus and Staphylococcal enterotoxins. Isolates were identified by biochemical, serological and molecular (PCR) methods. The obtained results in the present study revealed that (22%) of the examined samples were contaminated with Coliforms. The other identified genera were Citrobacter freundii, Citrobacter diversus, Edwardsiella tarda, Enterobacter spp., Morgonella morganii, Klebsiella oxytoca, Providencea spp., Serratia fonticola and Yersinia intermedia. E.coli (atypical type) was detected in a percentage of 27.27%. True fecal E. coli and Salmonella spp. failed to be detected in any of the whole examined 100 eggs samples. Additionally, Staphylococcus spp. was detected with incidence rate of (13%), out of them, 8 (61.5%) isolates were accounted for coagulase positive Staphylococcus aureus. Enterotoxin profiling revealed that two classical enterotoxin genes (SEA and SED) were detected either singly or in combination. The potential health hazards and the suggested control measures of the isolated strains had been discussed.