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Получение адгезивных антигенов возбудителя колибактериоза E.coli
2010
Zajtsev, V.V., Vitebsk Biofactory (Belarus) | Dremach, G.Eh., Vitebsk State Academy of Veterinary Medicine (Belarus) | Gorbunova, I.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Biletskij, M.O., Vitebsk Biofactory (Belarus)
In the conditions of the Republic of Belarus there was created an adhesive antigen on the basis of bacterial suspension of Escherichia which were separated from culture media by means of purification and concentration on membranes UMP-300 and UMP-5 that made it possible to obtain a cleaned-up preparation the specific activity of which was in 2-4 higher that of the unpurified one. In course of the study there was developed a method of purification and concentration of adhesive Escherichia antigens with the help of membrane technology. There were obtained biologically active adhesive antigens E.coli K88, K99, F41, 987P; there were selected the ultrafiltration membranes providing the optimal selectivity and productivity of the process; there were studied the influence of some factors on the process of adhesins concentration by means of ultrafiltration method. During the experiments there was analysed the agglutinating activity of antigents prepared by different methods.
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