With the exception of the domestic cat, all felid species (Felidae) are currently threatened with extinction in their natural habitat. To develop effective and optimal wild cat conservation programmes with assisted reproductive technology (ART) it is necessary to combine advances from different disciplines of science, starting from the biology of the species, through research into the population and habitat, assisted reproductive technologies, establishment of gene banks, developing bioinformatic systems, and ending with biodiversity and endangered species management. In the last few years knowledge of felid reproduction has expanded considerably thanks to comparative studies utilising the domestic cat as a research model for endangered wild cats. Basic reproductive techniques utilised in both domestic cat breeding and rescuing wild felid populations that are threatened with extinction include semen collection and cryopreservation, artificial insemination, oocyte collection, in vitro maturation, in vitro fertilisation, somatic cloning, and embryo transfer. The main directions in which assisted reproductive technologies are being developed in wild cat conservation implementations and the contribution of Polish research centres in advancing these methods are presented.

An ultrastructural study was to investigate on 2 cases of canine leiomyosarcoma. The nuclei of most tumor cells showed bilobate appearance. Most cells of the tumors had no distinct basement membrane. Most cells of the tumors had a degenerative rough endoplasmic reticulum. Intercellular matrix contained some amounts of collagen. Attachment areas between tumor cells were not apparent, but a few tight junction were clearly seen. Myofibrils were present in bipolar end area

The development of the dentistry was delayed in the preventive internal in wild animals. Some international papers regarding wild animal dentistry already had been carried through with animals originary from captivity and free life of some species of diverse geographic distributions, but not specific to the great neotropical felines (Panthera onca and Puma concolor). It was the aim of this paper to survey the prevalence of maloclusion in neotropical felines in the institutions visited in the State of São Paulo. For the study we used a sample of 42 jaguars (Panthera onca) and 36 pumas (Puma concolor), totalizing seventy and eight 78 animals , proceeding from 18 institutions of such species in captivity in the State of São Paulo. All animals had been examined, when it was observed if the occlusion was in accordance with the normal for the species. The teeth had been identified one by one, examined directly by means of dental explorer. The maloclusion was present in 47.61% (20 cases) in Panthera onca and two individuals of Puma concolor (5.55.00%). The animals with maloclusions apparently did not have problems of food apprehension or chewing ability, even though they presented in the region of teeth with more reduced interproximal space, thus plaque the lack of removal of this plaque can provoke oral problems. | O desenvolvimento da Odontologia Veterinária foi tardio na Clínica Médico Veterinária Preventiva de Animais Selvagens. Alguns trabalhos a respeito da Odontologia de Animais Selvagens já foram realizados com animais originários de cativeiro e vida livre de várias espécies de diversas distribuições geográficas, mas não específicos aos grandes felinos neotropicais (Panthera onca e Puma concolor). Objetivou-se neste trabalho fazer levantamento sobre a prevalência de maloclusão em grandes felinos neotropicais manejados nas instituições visitadas no Estado de São Paulo. Para os estudos utilizamos amostra constituída de 42 onças-pintadas (Panthera onca) e 36 suçuaranas (Puma concolor), totalizando 78 animais, provenientes de 18 instituições mantenedoras de tais espécies em cativeiro no Estado de São Paulo. Todos os animais foram examinados, observando-se se a oclusão estava de acordo com o normal para as espécies. Os dentes foram identificados um a um, examinados diretamente por meio de explorador odontológico. A maloclusão esteve presente em 47,61% (20 casos) em Panthera onca e dois indivíduos de Puma concolor (5,55.00%). Os animais que apresentaram maloclusões aparentemente não tinham problemas de apreensão de alimentos ou de mastigação, embora apresentassem acúmulo de alimentos ou indutos moles na região dos dentes com espaço interproximal mais reduzido, o que poderá levar a problemas futuros devido a falta de remoção desses indutos dos dentes.

O cloridrato de quetamina tem sido usado com freqüência na prática clínico-cirúrgica, devido à facilidade de aquisição, aplicação e à segurança da anestesia obtida com seu emprego. Este experimento objetivou identificar possíveis interferências do traçado eletrocardiográfico de gatos, submetidos ã associação de quetamina e clorpromazina. Foram utilizados 10 gatos, de ambos os sexos, adultos e considerados clinicamente sadios, provenientes do gatil do Hospital Veterinário da Faculdade de Ciências Agrárias e Veterinárias, campus de Jaboticabal-UNESP. Cada animal recebeu quetamina na dose de 15 mg/kg, associados a 1 mg/kg de clorpromazina, diluídos em uma mesma seringa e aplicados por via intramuscular. Foram avaliadas as freqüências cardíaca e respiratória e a temperatura retal. A avaliação eletrocardiográfica foi realizada nas derivações de membros, com velocidade de 50 mm/s e calibração de 1mv = 2cm, durante um período de 60 minutos. Dos resultados obtidos,<br />somente a temperatura retal, freqüência cardíaca e o intervalo P-R apresentaram diferenças significativas nos diferentes tempos observados. Da metodologia empregada permite-se concluir que a quetamina associada à clorpromazina pode ser empregada com segurança em gatos.<br /> | Ketamine HCL has frequently been used in clinical and surgical practice because of the easy acquisition, applicationand safety of the anesthesia it provides. The objective of the present experiment was to observe possible<br />interferences with the electrocardiographic tracing of cats submitted to the ketamine+chlorpromazine combination. The study was conducted in 10 adult cats of both sexes considered as clinically normal and provided by the animal house of the Veterinary Hospital, Faculty of Agrarian and Veterinary Sciences, campus of Jaboticabal-UNESP. Each animal received ketamine HCL at the dose of 15 mg/kg, in combination with 1 mg/kg chlorpromazine HCL, diluted in the same syringe and applied intramuscularly. The animals were then evaluated for heart and respiratory rates and rectal temperature. Electrocardiographic evaluation (ECG) was performed in the leads of the limbs, at a speed of 50 mm/s and with calibration of 1mv = 2cm, for a period of 60 minutes. Only rectal temperature, heart rate and P-R interval of the ECG presented significant differences at the different times observed. The methodology used permits us to conclude that ketamine in combination with chlorpromazine can be safety used in cats.<br />