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Characterisation of porcine enteropathogenic Escherichia coli isolated in northeastern India
2020
Kylla, Hosterson | Dutta, Tapan Kumar | Roychoudhury, Parimal | Subudhi, Prasant Kumar | Lalhruaipuii, | Lalsiamthara, Jonathan | Mandakini, Rajkumari
Enteropathogenic Escherichia coli (EPEC) is one of the main pathotypes causing gastroenteritis, particularly in young immunocompromised hosts. The study reports the prevalence, characterisation, and molecular epidemiology of EPEC from piglets in northeastern India. A total of 457 faecal samples were collected, from which 1,286 E. coli strains were isolated and screened by PCR. The resultant EPEC strains were serotyped and phenotypically characterised for resistance against 15 antimicrobials. Also, the phylogenetic sequence was analysed for 11 selected strains. A total of 42 strains (3.26%) belonged to atypical EPEC, of which, 15 (35.71%, and 2.29% of the 654 strains from this farm type) were isolated from organised and 27 (64.29%, and 4.27% of the 632 strains from this farm type) from unorganised farms; further, 5 (11.90% of the EPEC strains and 1.51% of the 330 strains from this breed) were isolated from the indigenous breeds and 37 (88.10%, and 3.87% of the 956 strains from this breed) from crossbred piglets. Serogroups O111 (11.9%) and O118 (7.14%) were the most prevalent of the 10 present. Sequence analysis of a length of the eaeA gene of 11 isolates of the region showed them to have 100% homology with each other and their identity ranged from 99.4% to 99.7% with GenBank reference sequences. All the EPEC isolates were multi-drug resistant, showing the highest resistance to amoxicillin (80.9%) and cephalexin (76.19%). The study highlighted the association of EPEC with piglet’s diarrhoea in northeastern India. EPEC isolates belonged to many serotypes and phenotypically all were multi-drug resistant with close genetic homology.
اظهر المزيد [+] اقل [-]Caprine coccidiosis in semi-arid India: Dynamics and factors affecting fecal oocysts count
2017
Dinesh Kumar Sharma | Souvik Paul | Pramod Kumar Rout | Ajoy Mandal | Saket Bhusan | Nitika Sharma | Yogendra Kumar Kushwah
Objective: This study envisages dynamics and factors affecting fecal oocyst counts (FOC) in natural infection in goats in semi-arid India.Materials and methods: A total of 1152 fecal samples from Jamunapari goats in semi-arid India were collected, processed and examined for fecal oocysts over a period of 3 years for prevalence and severity of the disease through FOC using modified Mc Master Technique. The log transformed FOC data on 912 animals from 59 sires were used for subsequent analyses for genetic and non-genetic factors affecting FOC. Fixed effects included were years of collection (1-3), seasons of collection (summer, rainy, winter), sex (male, female), age group (0-3, >3-6, >6-12, adults). Least squares analysis of variance for fitting constant was applied to data set.Results: The overall prevalence of coccidian infection in goats was 86.71%(n=999/1162). Highest incident was found in winter season (91.74%) and >6-12 M age (97.95%). Eimeria arloingi and E. ninakohlyakimovae were the most frequently occurring species. The heritability of FOC was found to be 0.06±0.06. The effect of sire on FOC was not significant; however, effects of animal age, year, season of collection and sex on FOC were found to be significant.Conclusion: Coccidiosis in goats is seasonally occurring disease, most commonly affecting animals of less than one year age. FOC in coccidiosis affected animals is lowly inherited trait for selection of goat against this disease. [J Adv Vet Anim Res 2017; 4(1.000): 52-57]
اظهر المزيد [+] اقل [-]Trypanosoma evansi causes thyroxin imbalance with biochemical alterations in wistar rats
2015
Sirigireddy Sivajothi | V. C. Rayulu | Bhavanam Sudhakara Reddy | Karumuri Nalini Kumari
Animals affected with Trypanosoma evansi show rare serum hormonal disturbances. One of the important hormones for livestock is thyroxin, and the level of thyroxin may be reduced during the T. evansi infection. The objective of the study was to investigate thyroxin level during experimentally induced T. evansi infection in Wister albino rats. Wistar albino white rats (n=12) were challenged with the local strain of T. evansi (at 5x105 trypanosomes/animal subcutaneously). At the high parasitemia, blood was collected from the rats, and serum was separated, which was subjected for biochemical evaluation. Decreased total serum thyroxin (2.91 ±0.04 µg/dL) and free thyroxin (1.30 ±0.05 ng/dL) levels (p<0.01) were recorded in T. evansi infected rats as compared to the control group of rats. Along with lowered thyroxin levels, decreased levels of total erythrocyte count, packed cell volume, hemoglobin, total leucocyte count, total serum proteins, albumin and glucose levels were recorded. On the other hand, significant increase (p<0.01) in cholesterol, blood urea nitrogen, serum alkaline phosphatase, serum aspartate aminotransferase, and serum alanine aminotransferase levels were observed. Thus, it is concluded that trypanosomiasis induces stress on rat, which have direct effect on thyroid hormone.
اظهر المزيد [+] اقل [-]Canine Helminthoses in Udgir: A Preliminary Prevalence Study
2018
Khillare, Bapurao | Bhangale, Gajendra
This study was carried out to investigate the gastrointestinal helminthes of dogs in Udgir area. Materials and This study consisted of 218 dogs over period of 10 months. A total of 218 faecal samples collected over a period of 10 months from dogs in Udgir area were subjected to fecal sample examination by standard parasitological procedures.An overall prevalence to the tune of 31.65% was noted. The major helminthes parasites recovered were Ancylostoma caninum (66.67%), Ancylostoma braziliense (4.35%), Toxocara canis (26.08%) and Dipylidium caninum (2.89%).There were non-significant differences in age wise and sex wise distribution of helminthes infecting dogs of the area.
اظهر المزيد [+] اقل [-]A NEW RECORD OF THE SQUAT LOBSTER MUNIDOPSIS CYLINDROPHTHALMA (ALCOCK, 1894), (CRUSTACEA : ANOMURA : GALATHEIDAE), OFF THOOTHUKUDI COAST OF GULF OF MANNAR (08º 35’ 22.5" N lat. 78º 27’ 40.9" E long and 08º 31’ 91.2" N lat. 78º 25’ 32.7"E)
2024
T. Vaitheeswaran
The squat lobster, Munidopsis cylindrophthalma (Alcock, 1894) was recorded for the first time from the Indian main land coast while investigating the biodiversity of the squat lobster of Gulf of Mannar during April, 2013. One specimen of the said species was collected as an incidental by-catch in the deep sea trawl fisheries off Thoothukudi coast of Gulf of Mannar, between 08º 35’ 22.5" N lat. 78º 27’ 40.9" E long and 08º 31’ 91.2" N lat. 78º 25’ 32.7"E long at a depth of 305-310 m. The present records show its extended distribution from Gulf of Mannar to east of Andamans and further to South China Sea.
اظهر المزيد [+] اقل [-]CARCASS CHARACTERISTICS OF NATIVE CHICKEN VARIETIES OF INDIA – REVIEW
2024
P. Shamsudeen | P. Thirunavukkarasu
The total meat production in India is 9.29 million out of which 4.78 million tonnes is from poultry and the growth of poultry meat sector has increased by 6.86 % over previous year. The growth rate of backyard poultry population from 2012 to 2019 (45.79 %) is ten times higher than when compared to commercial poultry (4.50 %) (BAHS, 2022). The native chickens are slowly growing in nature and are preferred for better flavor and for the belief that natural, less intensive management systems provide desi birds with higher welfare levels, resulting in much better product quality and great demand. The meat from native chicken is more delicious and provides nutritional security and economic support for the rural and most suited for backyard system of rearing. The aim is to study the carcass traits of available native chicken in India and compare it with native chicken at Hosur. The carcass characteristic of the native chicken variety at Hosur was evaluated by sacrificing 25 birds of each sex at 16 weeks of age. The observed results were compared with the performances of other varieties of native chicken in India with respect to the parameters like New York dressing yield, eviscerated yield, Ready to cook yield, cut-up parts yield, Meat bone ratio and Giblets yield at each sex of the bird at different age of slaughter especially at 16 weeks of age. It helps to identify the variety of native chicken which had good carcass traits and could be utilized for table delicacy.
اظهر المزيد [+] اقل [-]OCCURRENCE OF Dictyocaulus filaria INFECTION IN AN ORGANIZED SHEEP FARM OF TAMIL NADU, INDIA
2023
M. Prabhu | C. Sreekumar | N. Prema | P.C. Sakthivel | R. Anilkumar | M. Iyue
The sheep lungworm Dictyocaulus filaria is of world-wide distribution and causes verminous pneumonia which may result in weight loss and death of the infected sheep. The current investigation was carried out in an organized farm in the Nilgiris district of Tamil Nadu, India during the period from 2012 to 2014. Infected sheep showed clinical illness varying from dullness, depression, moderate coughing, dyspnoea and labored breathing with little mortality. On necropsy, both larval and adult stages of lungworm were recovered from trachea, bronchi and bronchioles. The identity of the nematodes was confirmed as D. filaria based on the socks shaped spicules in males and anterior knob in the larva. Of the 55 animals, including 17 lambs examined during the study period, eight animals were confirmed to have died due to verminous pneumonia. Apart from that, in a few lambs, lung abscess was noticed. Though the infection was present throughout the year, the clinical illness was pronounced in dry summer (n=23) and rainy season of south west monsoon (n=19). The disease was managed by minimizing the exposure of lambs to the contaminated pasture and by deworming with two doses of levamisole hydrochloride @ 7.5 mg/ kg body weight at 21 day interval to control the lungworm infection in sheep.
اظهر المزيد [+] اقل [-]Toxorhynchites-fluorescent antibody system for the detection of bluetongue virus from Culicoides midges (Diptera: Ceratopogonidae)
1997
Habibur Rahman, A. (Institute of Veterinary Preventive Medicine, Ranipet (India)) | Manickam, R.