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Iron-fortified recombinant Saccharomyces cerevisiae producing Sus scrofa ferritin heavy-chain recovers iron deficiency in mice
2012
Lim, H., Kangwon National University, Chuncheon, Republic of Korea | Kim, J.T., Kangwon National University, Chuncheon, Republic of Korea | Kim, M.D., Kangwon National University, Chuncheon, Republic of Korea | Rhee, K.J., Yonsei University, Wonju, Republic of Korea | Jung, B.D., Kangwon National University, Chuncheon, Republic of Korea
In this study, we produced iron-fortified yeast (Saccharomyces cerevisiae) producing Sus scrofa ferritin heavy-chain to provide iron supplementation in anemic piglets. We determined whether iron-ferritin accumulated in recombinant yeasts could improve iron deficiency in mice. C57BL/6 male mice exposed to Fe-deficient diet for 2 weeks were given a single dose of ferrous ammonium sulfate (FAS), ferritin-producing recombinant yeast (APO), or APO reacted with iron (Fe²+) (FER). The bioavailability of recombinant yeasts was examined by measuring body weight gain, hemoglobin concentration and hematocrit value 1 week later. In addition, ferritin protein levels were evaluated by western blot analysis and iron stores in tissues were measured by inductively coupled plasma spectrometer. We found that anemic mice treated with FER exhibited increased levels of ferritin heavy-chain in spleen and liver. Consistently, this treatment restored the iron concentration in these tissues. In addition, this treatment significantly increased hemoglobin value and the hematocrit ratio. Furthermore, FER treatment significantly enhanced body weight gain. These results suggest that the iron-fortified recombinant yeast strain is bioavailable.
اظهر المزيد [+] اقل [-]Effect of administration with fat-free diet and dietary iron on polyunsaturated fatty acid, vitamin E and their derivatives in male rats
1990
Huh, R.S. | Kim, Y.H. | Do, J.C. | Lee, Y.H. | Choi, Y.S. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Kim, S.H. (Korea Research Institute of Chemical Technology, Taejon (Korea Republic))
Effect of dietary vitamin E on the lipid peroxidation by dietary iron injected to male rats
1991
Huh, R.S. | Kim, Y.H. | Do, J.C. | Choi, Y.S. | Cheong, C.W. (Kyungpook National Univ., Taegu (Korea Republic). Coll. of Veterinary Medicine)
Studies on the safety of recombinant bovine somatotropin in dairy cow, 1; Effects of recombinant bovine somatotropin on mineral composition in milk of dairy cows
1991
Lee, M.H. (Seoul National Univ., Suwon (Korea Republic). Coll. of Veterinary Medicine) | Cho, T.H. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute)
