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Morphological And Histological Study of The Liver, Spleen and Pancreas in See-See (Ammoperdix griseogularis)
2020
Rasouli, Babak | Pourhaji Motab, Jalil | Rasouli, Seyyed Erfan
BACKGROUND: The importance of digestive accessory glands and spleen in human, domestic animals and birds has been studied for many years. OBJECTIVES: The aim of this study was to investigate the structural features of these organs in see-see. METHODS: Regarding the present study, 14 male and female see-see were randomly selected, from which, tissue samples of three males and three females were obtained. After preparation in the usual way, the samples were stained using hematoxylin and eosin methods. RESULTS: Liver involves two lobes, including right and left ones. Posterior border of the left lobe has a short cut which results in division of the left one into two sections. Gallbladder is extended in visceral level of the right lobe, from middle to posterior edge of it. Due to the lack of full penetration of capsule blades into the liver's parenchyma, lobulated tissues are unknown. Epithelium of the gallbladder is of simple cylindrical type; however, some areas there have very few goblet cells. See-see's spleen is red-brownish, pear-shaped, and is located in the posterior part of visceral level of the liver's left lobe. Spleen is embedded by muscle connective capsule. There are no splits of spleen capsule into parenchyma of the gland. Pancreas is an elongated organ in the situation between two arms of duodenum which is divided into three lobes including dorsal, ventral and splenic. Very delicate and short blades of the capsule are penetrated into the gland and it is divided to lobules. The endocrine part of pancreas consists of alpha and beta islets of Langerhans in large and small sizes. CONCLUSIONS: The results show that the structure and tissues of the liver, spleen and pancreas, despite the slight differences in morphological aspects, are similar to other birds.
اظهر المزيد [+] اقل [-]Effect of goose parvovirus and duck circovirus coinfection in ducks
2020
Liu, Jie | Yang, Xiaoxia | Hao, Xiaojing | Feng, Yongsheng | Zhang, Yuli | Cheng, Ziqiang
Coinfection of goose parvovirus (GPV) and duck circovirus (DuCV) occurs commonly in field cases of short beak and dwarfism syndrome (SBDS). However, whether there is synergism between the two viruses in replication and pathogenicity remains undetermined. We established a coinfection model of GPV and DuCV in Cherry Valley ducks. Tissue samples were examined histopathologically. The viral loads in tissues were detected by qPCR, and the distribution of the virus in tissues was detected by immunohistochemistry (IHC). Coinfection of GPV and DuCV significantly inhibited growth and development of ducks, and caused atrophy and pallor of the immune organs and necrosis of the liver. GPV and DuCV synergistically amplified pathogenicity in coinfected ducks. In the early stage of infection, viral loads of both pathogens in coinfected ducks were significantly lower than those in monoinfected ducks (P < 0.05). With the development of the infection process, GPV and DuCV loads in coinfected ducks were significantly higher than those in monoinfected ducks (P < 0.05). Extended viral distribution in the liver, kidney, duodenum, spleen, and bursa of Fabricius was consistent with the viral load increases in GPV and DuCV coinfected ducks. These results indicate that GPV and DuCV synergistically potentiate their replication and pathogenicity in coinfected ducks.
اظهر المزيد [+] اقل [-]Genetic relationship of Salmonella isolates found in subcutaneous abscesses in leopard geckos (Eublepharis macularius)
2020
Zając, Magdalena | Maluta, Aleksandra | Wasyl, Dariusz | Skarżyńska, Magdalena | Lalak, Anna | Samcik, Ilona | Kwit, Renata | Szulowski, Krzysztof
The article describes the occurrence and phylogenetic relationship of Salmonella isolates found in subcutaneous abscesses of leopard geckos. The aim of the study was to determine the cause of the abscesses and to characterise isolated Salmonella strains. Samples of abscesses from five animals and internal organs (lungs, liver, and gut) of three of them were tested for Salmonella according to the PN-EN ISO 6579:2002/A1:2007 standard. The antimicrobial resistance was evaluated by minimal inhibitory concentrations and the genetic similarity of the isolates was assessed with pulsed field gel electrophoresis (PFGE). In total, seventeen Salmonella isolates belonging to five different serovars were found to be susceptible to all tested antimicrobials except streptomycin. The serovars were S. Hadar, S. Fluntern, S. Tennessee, S. enterica subsp. salamae 55:k:z₃₉, and S. Kentucky. Up to three serovars from different organs were isolated from the same individual. In two geckos, Salmonella were detected in the lungs. In three serovars, XbaI-PFGE typing revealed indistinguishable isolates from organs and abscesses. Multiple Salmonella serovars might be involved in abscess formation and infections. The occurrence of the same PFGE profiles of the isolates may testify to the role of opportunistic organisms in causing infection.
اظهر المزيد [+] اقل [-]Occurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018
2020
Marek’s disease (MD) is a tumourous disease caused by Marek’s disease virus (MDV) and most commonly described in poultry. The aim of the study was to determine the occurrence of Marek’s disease virus infections in Poland and analyse clinical cases in the years 2015–2018. The birds for diagnostic examination originated from 71 poultry flocks of various types of production. Birds were subjected to anatomopathological examination post mortem, during which liver and spleen sections and other pathologically changed internal organs were taken. These sections were homogenised with generally accepted methods, then total DNA was isolated and amplified with a real-time PCR. A pair of primers complementary to the MDV genome region encoding the meq gene were used. MDV infection was found predominantly in broiler chicken flocks (69.01%), and also in layer breeder (9.85%) and commercial layer flocks (7.04% each). The results of research conducted in the years 2015–2018 clearly indicate that the problem of MDV infections is still current.
اظهر المزيد [+] اقل [-]Characterisation of fowl adenovirus (FAdV-8b) strain concerning the geographic analysis and pathological lesions associated with inclusion body hepatitis in broiler flocks in Turkey
2020
Cizmecigil, Utku Yusuf | Umar, Sajid | Yilmaz, Aysun | Bayraktar, Erhan | Turan, Nuri | Tali, Besim | Aydin, Ozge | Tali, Hasan Emre | Yaramanoglu, Mehmetcan | Yilmaz, Semaha Gul | Kolukisa, Ahmet | Sadeyen, Jean-Remy | Munir, Iqbal | Yılmaz, Hüseyin
Fowl adenovirus can cause important diseases in chickens such as inclusion body hepatitis, hepatitis hydropericardium syndrome, and gizzard erosion and ulceration. Inclusion body hepatitis has been regularly reported from many countries. This is the first case report from Turkey, describing an outbreak of inclusion body hepatitis in broiler farms due to fowl adenovirus-8b (FAdV-8b). Broiler flocks with mortality about 10% were visited in Turkey, and necropsy was performed on dead birds. Samples were subjected to PCR assay to detect FAdV and other viral pathogens. After sequencing, phylogenetic analysis was performed and the nucleotide sequences of hexon genes were compared with the FAdV sequences data available in GenBank. Clinical signs such as anorexia, depression, ruffled feathers, huddling, and greenish diarrhoea were observed. Mortality started at the 8ᵗʰ day of age and ranged from 10% to 14%. Necropsy showed severe hepatitis, jaundice, and pancreatitis. The main necropsy findings included a pale, enlarged, haemorrhagic, and friable liver along with swollen and haemorrhagic kidneys and spleen. PCR and sequence analysis revealed the presence of fowl adenovirus serotype 8b (FAdV-E). This is the first report on characterisation and the pathological lesions associated with FAdV in broilers in Turkey. Our findings suggest that FAdV strains could be an emerging pathogen in Turkish broilers and could actively contribute to hepatitis and immunosuppression.
اظهر المزيد [+] اقل [-]Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland
2020
Hofsoe-Oppermann, Paulina | Kiełpińska, Jolanta | Panicz, Remigiusz | Bergmann, Sven M.
White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions, i.e. high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for quick and efficient confirmation or exclusion of the presence of WSIV. A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon (Acipenser gueldenstaedtii and A. oxyrinchus) aged ≤5+ farmed or caught between 2010 and 2014 in open waters (Dąbie Lake and Szczecin Lagoon). They were tested for WSIV presence using conventional PCR, qPCR, and in situ hybridisation (ISH). In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA. The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection.
اظهر المزيد [+] اقل [-]Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing
2020
Escárcega, Dulce Anahy Verdugo | Razo, Claudia Angélica Perea | Ruíz, Sara González | Gallegos, Susana Lucia Sosa | Suazo, Feliciano Milián | Alarcón, Germinal Jorge Cantó
Bovine tuberculosis, caused by M. bovis, is endemic in Mexico and has had a big impact on public health. Jalisco is considered to be an important dairy region in the country, accounting for approximately 19% of the total milk production. Within Jalisco, the region of Altos Sur holds the largest proportion of the cattle inventory of the state. To determine the frequency of bovine tuberculosis in Altos Sur, Jalisco, as well as M. bovis genetic diversity, sampling of tissue (lymph nodes, lungs, and liver) from Holstein cattle was performed in four abattoirs belonging to three municipalities of this region (Tepatitlán de Morelos, San Miguel el Alto, and Arandas). Spoligotyping and whole-genome sequencing were carried out to assess the genetic relationships of M. bovis strains circulating in this area, as well as a comparison to isolates from other places in Mexico. Prevalence was 15.06%, and distribution similar among the three municipalities. The most frequent spoligotypes were SB0673, SB121, and SB0145. Whole-genome sequencing revealed three main clades (I, II, III), but isolates did not show clustering by region. Phylogenetic analysis suggested ongoing transmission between herds of the different regions, and no unique source of infection was determined. This hinders efforts under the national program for the control and eradication of the disease, so serious attention must be paid to rural regions such as Altos Sur in order to improve its success.
اظهر المزيد [+] اقل [-]An immunohistochemical study on the presence of nitric oxide synthase isoforms (nNOS, iNOS, eNOS) in the spinal cord and nodose ganglion of rats receiving ionising gamma radiation to their liver
2020
Yılmaz, Osman | Soygüder, Zafer | Keleş, Ömer Faruk | Yaman, Turan | Yener, Zabit | Uyar, Ahmet | Çakır, Tahir
This study determined the presence of nitric oxide synthesis isoforms (nNOS, iNOS, and eNOS) in thoracic spinal cord segments and nodose ganglia of rats with gamma-irradiated livers. Male rats (n = 32) were divided into equal groups A, B, C, and D. In group A, the controls, no radiation was applied, while groups B, C, and D received 10 Gy of ionising gamma radiation. The rats of group B were euthanized at the end of the first day (d1), those of group C on the second day (d2), and those of group D on the third day (d3). The liver, spinal cord segments, and nodose ganglion tissues were dissected and fixed, and the liver sections were examined histopathologically. The other tissues were observed through a light microscope. Regeneration occurred at the end of d3 in hepatocytes which were radiation-damaged at the end of d1 and d2. On d1, some nNOS-positive staining was found in the neuronal cells of laminae I–III of the spinal cord and in neurons of the nodose ganglion, and on d3, some staining was observed in lamina X of the spinal cord, while none of note was in the nodose ganglion. Dense iNOS-positive staining was seen on d1 in the ependymal cells of the spinal cord and in the glial cells of the nodose ganglion, and on d3, there was still considerable iNOS staining in both tissues. There was clear eNOS-positive staining in the capillary endothelial cells of the spinal cord and light diffuse cytoplasmic staining in the neurons of the nodose ganglion on d1, and on d3, intense eNOS-positive staining was visible in several endothelial cells of the spinal cord, while light nuclear staining was recognised in the neurons of the nodose ganglion. The nNOS, iNOS, and eNOS isoforms are activated in the spinal cord and nodose ganglion of rats after ionising radiation insult to the liver.
اظهر المزيد [+] اقل [-]Hypoglycemic and hepatoprotective effect of Rhizophora mucronata and Avicennia marina against streptozotocin-induced diabetes in male rats
2020
Obidallah Hamdan Ali Al-Jaghthmi | Isam ELDin Mohamed ELAmin Abu Zeid
Objectives: Aqueous extracts of Rhizophora mucronata and Avicennia marina leaves were inves¬tigated for their hepatoprotective potential in diabetic rats. Materials and methods: One hundred twenty male albino rats were randomly assigned to eight equal groups (n = 15). The first group (control) comprised normal healthy rats, while the second to fifth groups were intraperitoneally injected with a single dose of streptozotocin (STZ) [60 mg/ kg body weight (BW)] for induction of diabetes. Group 2 was kept as positive diabetic control, while groups 35 were orally treated with aqueous extracts of R. mucronata (400 mg/kg BW), A. marina (400 mg/kg BW) and with a combination of ½ a dose of the two plants, respectively, for six weeks. Groups 68 were non-diabetic rats that orally received aqueous extracts of R. mucronata (400 mg/kg BW), A. marina (400 mg/kg BW), and a combination of ½ a dose of the two plants, respectively, for 6 weeks. Results: STZ-induced diabetic rats showed a significant reduction in serum glucose and liver enzymes, increased serum insulin, Homeostasis Model Assessment of β-cells (HOMA-β), and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR). Histopathological and immuno¬histochemical examinations of the liver revealed improved pathologic criteria in the plant extract treated diabetic rats compared with the remarkable changes which had been seen in STZ-induced diabetic rats. Conclusion: This study suggests that the aqueous extract of R. mucronata or its combination with A. marina showed potent hypoglycemic and hepatoprotective effects for liver dysfunction, as well as histopathological and immunohistochemical changes in the liver of STZ-induced diabetic rats. [J Adv Vet Anim Res 2020; 7(1.000): 177-185]
اظهر المزيد [+] اقل [-]Size of canine hepatocellular carcinoma as an adverse prognostic factor for surgery
2020
Yury Vatnikov | Ilya Vilkovysky | Evgeny Kulikov | Irina Popova | Nadia Khairova | Aleksey Gazin | Andrey Zharov | Darya Lukina
Objective: Liver neoplasms are problematic among small domestic animals. The etiological cause of hepatocellular carcinomas in domestic animals is still unknown although it is believed that chronic infections and toxic substances can affect the development of this type of tumor. This study aimed to analyze the clinical and morphological characteristics of canine hepatocellular carcinoma. Materials and methods: In total, 6,958 cancer operations were performed in the clinic. Liver tumors were detected in 123 dogs in vivo and 375 dogs postmortem. All animals with suspected liver neoplasm were assessed, including history, clinical examination, complete blood count, biochemical blood tests, radiographic examination, and ultrasound with a biopsy for performing cytological and histological analyses. Results: Hepatocellular carcinomas have nonspecific clinical manifestations, also a characteristic aspect of other tumors of the hepatobiliary system. The hematological changes have an impact on the prognosis, and biochemical abnormalities reflect the changes in liver activity. The cytological diagnosis of hepatocellular tumors is difficult because of hepatocyte atypia in highly differenti¬ated carcinomas. Finally, a histological examination was performed in all the dogs diagnosed with hepatocellular carcinoma. Conclusion: Hematological changes in dogs with hepatocellular carcinoma affect their prognosis. Biochemical abnormalities of this pathology reflect the changes in liver activity, not indicating a specific pathology. However, an increase in the activity of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase is an unfavorable prognostic sign. In this study, five of seven dogs with a tumor size of more than 5.0 cm had a life expectancy of 30, 51, and 91 days, suggesting that the size of the tumor is an adverse prognostic factor. [J Adv Vet Anim Res 2020; 7(1.000): 127-132]
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