Six primiparous Holstein cows were fed a Se-deficient diet, beginning at least 90 days before their first calving, and 6 other primiparous cows were given the same diet plus a supplement of 2 mg of Se/cow/d as sodium selenite. All cows were fed their diets for the duration of the experimental period. One uninfected quarter of each cow was injected with 25 microgram of Escherichia coli endotoxin at postpartum week 5. Leukocytes were isolated by centrifugation from milk collected at postinjection hour 16. Isolated cells were 92 +/- 3% neutrophils and were incubated with Staphylococcus aureus or E coli in a 1:300 ratio. Phagocytosis and intracellular killing by neutrophils were assessed after 0, 30, 60, and 90 minutes by a fluorochrome assay, using acridine orange. Viability of neutrophils was assessed by use of trypan blue. Superoxide anion production and hydrogen peroxide production by neutrophils also were determined. Cows fed Se-deficient diets had significantly (P < 0.05) lower blood Se concentration and blood glutathione peroxidase activity than cows fed Se-supplemented diets. Selenium status had no effect on the phagocytic capacity of neutrophils. Neutrophils obtained from cows fed Se-supplemented diets killed a significantly (P < 0.05) higher percentage of ingested bacteria than did neutrophils from cows fed the Se-deficient diet. Viability was significantly (P < 0.05) reduced by incubation with S aureus in neutrophils from both groups of cows, with neutrophils from Se-deficient cows having lower viability. Superoxide anion production did not differ significantly between neutrophils from the 2 groups, but extracellular hydrogen peroxide concentration was significantly (P < 0.05) higher in neutrophils harvested from milk of cows fed the Se-deficient diet.

This study was designed to elucidate the effects of iron, a well known catalyst of lipid peroxidation, on the contents of phospholipids, unsaturated fatty acids composed in phospholipid molecules and their derivatives, prostaglandins, and the composition changes of fatty acids contained in phospholipids. Iron decreased the contents of phospholipids and its components of unsaturated fatty acids. Catalytic action of iron decreased the composition rates of linoleate and linolenate composed in phospholipid molecules, while that of arachidonate was inclined to increase. The content of arachidonate was increased and that of prostaglandins was decreased without regard to increase the precursor of prostaglandins. It may be concluded that the decreases of the activities of prostaglandins and the increase of arachidonate are due to inhibition of the activities of enzyme systems responsible for prostaglandin synthesis by lipid peroxides produced by the catalyst of iron

In the conditions of the Republic of Belarus there was analyzed the influence of mycotoxins on state of energy metabolism and development of energy deficient states of bred and milking sows maintained in the production breeding conditions. Clinical status of sows was characterized by concurrent mycotoxicoses. As a result of action of mycotoxins in sows there was developed an energy deficiency and state of acidosis. The mentioned above changes played an important part in the pathogenesis of mycotoxicosis. There were presented the following results: analysis of groups of pregnant sows and suckling-pigs; the content of mycotoxins in mixed fodders; symptoms revealed at sows and pigs; indexes of carbohydrate and lipide exchanges in blood of sows; the content of trace elements in blood of sows; gas composition of sow blood. | Исследования проведены на супоросных и подсосных свиноматках, содержащихся в условиях промышленной технологии. Клинический статус свиноматок характеризовался сочетанным микотоксикозом. В результате действия микотоксинов у свиноматок развились энергодефицит и состояние ацидоза. Данные изменения являются важной составной частью патогенеза при микотоксикозах. В табличной форме представлены группы супоросных и подсосных свиноматок, содержание микотоксинов в комбикормах, симптомы, выявленные у свиноматок и поросят, показатели углеводного и липидного обменов в крови свиноматок, содержание макроэлементов в крови свиноматок, газовый состав крови свиноматок.

In the conditions of the Republic of Belarus there was realized the determination of efficiency of application of saline and chelate forms of copper and cobalt for treatment of anemic syndrome of cattle. It was found out that lack of copper and cobalt in cattle diets in the conditions of normal supply of iron could be a cause of cattle anemic syndrome. It took place because of the iron adsorption disorder in intestines and its inclusions in haem, as well as lowering of ehritropoztin synthesis. In the conditions of administration of copper and cobalt-containing preparations for animals there was noted the increasing of iron and haemoglobin content in blood. Administration of ethylene diamine tetraacetates of cobalt and copper made it possible to reduce considerably all evidences of clinical and laboratory symptoms of anemia and hypomicroelementosis in comparison with application of copper sulphate and cobalt.

In the conditions of the Republic of Belarus clinic and production conditions there was analyzed the prophylactic and therapeutic efficacy of a new national complex iron dextrane preparation Ferovital against deficiency (nutritional) anemia of new-born cattle and swine. In course of the study there were analyzed results of hematological and biochemical tests of blood of piglets which were administrated with Ferovital preparation. There were also marked the general economic index: live weight gain, disease incidence, piglet death rate, etc. Research results showed that the Ferovital preparation had an expressed prophylactic and therapeutic efficacy both in clinic and production conditions.

The influence of iron deficiency state on metabolic processes and intensity of lipids peroxidation processes at dawn-calving cows was analysed in the conditions of the Republic of Belarus. In course of study there was determined the intensity of lipids peroxidation processes in blood plasm depending on the level of hemoglobin and red blood corpuscle quantity. It was studied that in animals with low hemoglobin the oxidizing stress proceeded more actively than in animals with the normal hemoglobin level. Data analysis of lipids peroxidation processes showed that pregnancy condition was accompanied by strengthening of mentioned above processes. That fact was proved by the indicators of lipids peroxide oxidations presented both in absolute and in relative units. The content of diene conjugates, containing in 1 mg of lipids, was in 1,3-1,8 times higher at pregnant cows in comparison with non-pregnant ones, trieneketones content - in 1,2-1,6 times. Similar differences were observed in the process of analysis of diene conjugates and trieneketones values rated per 1 ml of plasma. More considerable differences were stated for trieneketones; the analysed indicator was in 1,6-2,1 times higher at pregnant cows

Development of new selenium preparations in a complex with vitamins for the purpose of decrease of trace element toxicity and increase of its bioavailability is the topical problem of veterinary science. A new antioxidant water-soluble complex Aesel, which includes selenium as the activated product of interaction of methionine and sodium selenite, vitamins A and E, is researched. Lethal doses of the preparation for mice and rats were identified through intragastric and subcutaneous introduction. Due to the results of the research it was determined that Aesel is rated among the forth class of danger, so it is dangerous to a very little degree. Lethal doses of the preparation on the pure selenium, calculated on Pershin method, for rats at parenteral introduction is equal 5,48, at enteral introduction - 39, for mice at parenteral introduction - 7,72 mg/kg. Lethal doses of the preparation calculated on all making components equal to 39000 mg/kg.

There was studied the width of distribution and peculiarity of clinical manifestation of mineral exchange diseases at pregnant sows, and also perfection of preventive actions at pathology of mineral metabolism in the conditions of prophylactic medical examination. Results of research have shown, that diseases of mineral metabolism widespread (84,7%) among pregnant sows in the conditions of industrial production, are most extended scarce conditions on Zn (17,7%) and Fe (22,5%). Clinically this insufficiency was expressed by oppression, appetite distortion, occurrence on a skin of specifically red stains correct roundish the form: with the clarified sites in the centre, are registered as abortions at final stages of pregnancy. It is established, that the way of preventive maintenance of hypomicroelementosis of pregnant sows with use of chelated forms of microelements is more preferable than sows, as reduces percent of clinical display of Zn and Fe deficiency, stabilize level of hematological and biochemical indicators of blood more effectively, and also raise industrial indicators of pregnant sows. | Целью работы было изучение широты распространения и особенностей клинического проявления заболеваний минерального обмена у супоросных свиноматок, а также совершенствование профилактических мероприятий при патологии минерального обмена веществ в условиях диспансеризации. Результаты исследований показали, что болезни минерального обмена веществ широко распространены (84,7%) среди супоросных свино¬маток в условиях промышленного производства, наиболее распространены дефицитные состояния по Zn (17,7%) и Fe (22,5%). Клинически эта сочетанная недостаточность выражалась угнетением, залеживанием, понижением и извращением аппетита, появлением на коже специфически красных пятен правильной округлой форма: с просветленными участками в центре, зарегистрированы так же аборты на конечных стадиях супоросности. Установлено, что способ профилактики гипомикроэлементозов супоросных свиноматок с использованием хелатных форм микроэлементов предпочтительнее, поскольку сокращает процент клинического проявления дефицита цинка и железа, эффективнее стабилизируют уровень гематологических и биохимических показателей крови, а также повышают производственные показатели супоросных свиноматок.

Belarus is among regions, where the selenium content in soil, hence, in cereal and other cultures considerably below requirements that leads to insufficiency of this microelements in a diet as the person, and animals. Synthesized in the All-Russian Scientific Research Institute of Physiology, Biochemistry and Feeding of Agricultural Animals new organic compound of selenium - selenopyran on variety of criteria has no analogues in the world among considerable quantity selenium of containing preparations and favourably differs from all known forms of microelements. Selenopyran, brought in fats and forages, shows antioxidatic the properties which are not conceding traditionally applied in veterinary science and medicine to antioxidants. Arriving in animal body with forage or in the form of injections, selenopyran is capable to play a role of a metabolic regulator. It activates enzymes of antioxidatic body protection, reduces formation new and will neutralize of earlier formed activeproducts of peroxide lipids oxidations, improves functioning of cellular membranes, normalizes metabolism, makes active cellular, humoral and phagocytal immunity links, raises nonspecific resistance and efficiency of animals. Toxicity of selenopyran more low, than at all known organic connections of selenium, and more than in 100 times is less, than at sodium selenit. The data allow to expand possibilities of application of organic selenium and to use it as a preparation raising resistance and body viability at the expense of the patronage of cellular structures from influence of oxidizers and free radicals, to normalize metabolism and to stimulate thus the big additional body weight at animals. The influence of medicine organic selenium on piglet bodies what are behind a growth and development is shown. The results showing the dynamical changes of haematologicals, physiologicals and biochemicals indices under the influence of selenopyran was given.