Experiments were designed to clarify the effect of different extenders on sperm motility of rainbow trout Oncorhynchus mykiss after shorttermcold storage at 4°C for 6 days. Sperm collection was performed through gentle abdominal massage. Sperm was suspended in differentextenders at 1:3 dilution ratio. The motility and survival of sperm cells were assessed in all the treatments daily. Our results indicated that spermremained as motile in glucose and dimethyl sulphoxide (DMSO) based extender at day 6. This study would be beneficial for cryopreservationand reproduction management.

In this study, trout (Oncorhynchus mykiss) from the facilities located in Doganşehir Sürgü, Malatya was used. Samples were received betweenNovember 2017 and January 2018. During the study, a total of 60 fish, 10 female, 10 male, 10 female, 10 male and 10 female, were studiedin the pre-milking period. After the fish were anesthetized with 0.25% phenoxyethanol, blood was taken from the tail end with the help of theinjector. Blood samples were taken from the alveolar vessels and measured with the PROKAN 6800 VET device. Hematological analyzes wereperformed using the Cell DN 1700 coulter counter to determine blood parameters. The weight of male fishes before mating was 359.5 ± 32.7g, increased to 453 ± 74.09 g during the mating period and decreased to 354 ± 99.35 g at the end of mating period. In females, before matingperiod weights were 421 ± 28.46 g, decreased to 375 ± 54.62 g during the mating period and increased to 392 ± 75.69 g after the mating period.The blood parameters obtained for male fish are as follows; white blood cells (WBC): 50.8 ± 14.82 103/μL before mating, 55.79 ± 2.79 103/μL during mating, 54.61 ± 3.052 103/μL after mating; red blood cells (RBC): 1.95 ± 0.59 106/μL before mating, 2.25 ± 0.20 106/μL duringmating, 2.24 ± 0.35 106/μL after mating; hemoglobin (HGB): 10.11 ± 3.07 g/dl before mating, 11.02 ± 1.02 g/dl during mating, 9.79 ± 0.84g/dl after mating; hematocrit (HCT): 26.38 ± 8.04 % before mating, 28.73 ± 2.70 % during mating, 29.62 ± 3.77 % after mating; leucocyte(GRAN): 1.664 ± 0.81 103/μ before mating, 1.22 ± 0.29 103/μ during mating, 1.33 ± 0.36 103/μ after mating. The blood parameters obtainedfor female fish are as follows; white blood cells (WBC): 54.57 ± 4.86 103/μL before mating, 47.2 ± 8 103/μL during mating, 42.65 ± 7.11 103/μL after mating; red blood cells (RBC): 1.94 ± 0.18 106/μL before mating, 1.75 ± 0.33 106/μL during mating, 1.39 ± 0.33 106/μL after mating;hemoglobin (HGB): 10.17 ± 0.78 g/dl before mating, 8.89 ± 1.59 g/dl during mating, 6.74 ± 1.57 g/dl after mating; hematocrit (HCT): 26.18 ±2.07 % before mating, 22.44 ± 4.54 % during mating, 18.04 ± 4.03 % after mating; leucocyte (GRAN): 1.54 ± 0.47 103/μ before mating, 1.18± 0.26 103/μ during mating, 1.09 ± 0.22 103/μ after mating.

Aeromonas hydrophila is a Gram negative, positive oxidase, anaerobic, and opportunistic bacteria that, under certain conditions, become a pathogen (in humans and fish). This bacterium causes toxin and host infection in which different antibiotic resistance in isolated strains has been reported in different regions of the world. The aim of this study was to determine the prevalence of this bacterium and its susceptibility to common antibiotics in Tabriz city.50 samples from 5 Reared Oncorhynchus mykiss farms in Tabriz city (For each farm,10 numbers) were randomly assigned to suspected fish to the disease. By using biochemical tests, 14 samples (28%) from 2 Fish farms (40%) were identified as A.hydrophila. Antibiogram for these specimens showed that the bacterium had the highest resistance tovancomycin (100 %) and clindamycin (92.8%) antibiotics, and has the most sensitivity to the antibiotics ofsultrim, tetracycline and oxytetracycline with 71.4%.Considering the different antibiotic resistance pattern in this study and other similar studies,the necessity of examining the pattern of resistance in each region seems necessary.