خيارات البحث
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Case report of Johnes disease in Korean black goat (Capra hircus aegagrus)
2013
Rhyoo, M.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jung, J.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, K.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Her, J.W., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Bae, Y.C., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, H.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Baek, K.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, B.R., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jean, Y.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, J.M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, M.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Yoon, S.S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Two Korean black goat (approx. 2 and 3 years old) showing diarrhea and chronic weight loss were submitted to Animal and Plant Quarantine Agency. At necropsy, there were thickening of small intestine and enlargement of mesenteric lymph nodes. Microscopically, they had granulomatous enteritis in the small and large intestine and granulomatous lymphadenitis. By polymerase chain reaction (PCR) and acid fast stain, strong positive reaction and acid-fast rod bacteria were detected. According to the result of histopathology and PCR, we confirmed
اظهر المزيد [+] اقل [-]Development of ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis with truncated 34 kDa proteins
2006
Malamo, M.(Hokkaido Univ., Sapporo (Japan)) | Sakoda, Y. | Ozaki, H. | Kida, H.
To develop ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis), the carboxyl termini of the 34 kDa proteins of M. paratuberculosis and Mycobacterium avium subsp. avium (M. avium) were expressed in Escherichia coli expression system. Antibodies specific to M. paratuberculosis were detected with the truncated 34 kDa protein of M. paratuberculosis in ELISA after pre-absorption of serum samples with the truncated 34 kDa protein of M. avium. All the serum samples from cattle confirmed to be infected with M. paratuberculosis were positive and those from healthy cattle were negative in the present ELISA system. These results indicate that the established ELISA detects antibodies specific to M. paratuberculosis with high specificity and sensitivity and is an useful tool for the screening of Johne's disease.
اظهر المزيد [+] اقل [-]A survey of paratuberculosis by immunological methods in dairy and Korean native cattle
1994
Kim, J.M. | Ahn, J.S. | Woo, S.R. | Jo, D.H. | Jo, Y.S. | Park, J.M. | Yoon, Y.D. (Rural Development Administration, Anyang (Korea Republic). Veterinary Research Institute) | Chang, G.H. (Kangwon Veterinary Service Laboratory, Chunchon (Korea Republic). Chungbu Branch)
Studies on molecular biological and immunological diagnosis of johne's disease
1997
Kim, T.J. | Kim, Y.S. | Kim, J.C. | Yoon, W.J. | Lee, W.C. (Konkuk University, Seoul (Korea Republic). College of Animal Husbandry) | Shin, S.J. | Chang, Y.F. (Cornell University (USA). College of Veterinary Medicine)
Spontaneous paratuberculosis in a sika deer:a case report
1993
Bae, J.H. (Cheju National University, Cheju (Korea Republic). Department of Veterinary Medicine) | Jean, Y.H. (Rural Development Administration, Cheju (Korea Republic). Veterinary Research Institute)
