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Molecular characterization of MHC II DRB3 gene of swamp- and riverine-type water buffaloes
2019
Noraine P. Medina | Arren Christian M. De Guia | Virginia M. Venturina | Claro N. Mingala
Objective: Major histocompatibility complex (MHC) is a set of molecular proteins on the surface of antigen presenting cells encoded by a large gene family which are important parts of the immune system. This study was conducted to convey information on the genetic characteristics of the MHC II DRB3 gene in riverine and swamp buffaloes. Materials and Methods: Characterization of MHC II DRB3 gene was carried out using polymerase chain reaction (PCR)-based assay. Thirty-milliliter milk samples were collected from 10 swamp-type and 10 riverine-type buffaloes. RNA from milk samples were extracted using Trizol and then followed by reverse transcription-PCR (RT-PCR). Results: The phylogenetic analysis with 1,000 bootstrap replications clearly showed complex parsimony in MHC II DRB3 gene between 10 riverine- and 10 swamp-type but also confirmed that the samples are similar to Bubalus bubalis. Aligned sequences of the 20 water buffaloes were compared with three other ruminants (Bos taurus, Ovis aries, and Capra hircus) and non-ruminant (Sus scrofa) that serve as an outgroup. MHC sequences from GenBank show that there was an average of 705 identical pairs, with 22 transitional pairs and 30 transversional pairs with a ratio of 0.7. Conclusion: Based on the molecular data, the current study conforms to other works of literature that this gene is highly polymorphic which can be due to its function in the immune responsiveness and disease resistance. Further study on the immunological response of MHC II DRB3 to infection may elucidate its underlying function and role in the protection against specific disease of animals. [J Adv Vet Anim Res 2019; 6(3.000): 308-314]
اظهر المزيد [+] اقل [-]Molecular detection and genetic characterisation of pathogenic Theileria, Anaplasma and Ehrlichia species among apparently healthy sheep in central and western Kenya
2019
Ringo, Aaron E.(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Aboge, Gabriel O.(University of Nairobi Faculty of Veterinary Medicine Department of Public Health, Pharmacology and Toxicology) | Moumouni, Paul F. Adjou(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Lee, Seung Hun(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Jirapattharasate, Charoonluk(Mahidol University Faculty of Veterinary Science Department of Pre-clinic and Applied Animal Science) | Liu, Mingming(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Gao, Yang(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Guo, Huanping(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Zheng, Weiqing(Nanchang Center for Disease Control and Prevention Department of Disinfection and Vector Control) | Efstratiou, Artemis(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Galon, Eloiza M.(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Li, Jixu(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Thekisoe, Oriel(North-West University Unit for Environmental Sciences and Management) | Inoue, Noboru(Obihiro University of Agriculture and Veterinary Medicine) | Suzuki, Hiroshi(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases) | Xuan, Xuenan(Obihiro University of Agriculture and Veterinary Medicine National Research Center for Protozoan Diseases)
Tick-borne diseases (TBDs) caused by Theileria, Babesia, Anaplasma and Ehrlichia species are common in tropical and subtropical regions. In this study, we investigated the presence and genetic diversity of Theileria spp., Anaplasma ovis, B. ovis, E. ruminantium and Anaplasma spp. in sheep from the Machakos and Homa Bay counties of Kenya. In order to improve the diagnosis and control of ovine TBDs, a total of 76 blood samples from apparently healthy sheep were screened using a polymerase chain reaction (PCR). The assays were conducted using primers based on Theileria spp. 18S rRNA, Anaplasma ovis Major surface protein-4 (AoMSP4), B. ovis 18S rRNA, E. ruminantium pCS20 and Anaplasma spp. 16S rRNA. The overall infection rates for Theileria spp., A. ovis, E. ruminantium and Anaplasma spp. were 39/76 (51.3%), 26/76 (34.2%), 6/76 (7.9%) and 31/76 (40.8%), respectively. The overall co-infection was 47/76 (61.8%). All Theileria spp. positive samples were confirmed to be of Theileria ovis on sequencing. A phylogenetic analysis of the 18S rRNA gene sequences of T. ovis revealed that all isolates of this study clustered with T. ovis sequences extracted from the GenBank suggesting this gene is highly conserved. E. ruminantium pCS20 sequences were in the same clade on the phylogenetic tree. However, three AoMSP4 sequences from this study appeared in the same clade, while one sequence formed a separate branch revealing genetic divergence. The 16S rRNA sequencing revealed uncharacterised Anaplasma spp. and A. ovis. The phylogenetic analyses of the uncharacterised Anaplasma spp. revealed that the two sequences from this study appear in an independent clade from other sequences extracted from the GenBank. This study provides important information regarding the occurrence of tick-borne pathogens and their degree of genetic diversity among sheep in Kenya, which is useful for the diagnosis and control of TBDs.
اظهر المزيد [+] اقل [-]Molecular characterisation of infectious bursal disease virus in Namibia, 2017
2019
Molini, Umberto(University of Namibia Faculty of Agriculture and Natural Resources School of Veterinary Medicine) | Aikukutu, Gottlieb(Central Veterinary Laboratory) | Kabajani, Juliet(International Atomic Energy Agency Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) | Khaiseb, Siegfried(Central Veterinary Laboratory) | Cattoli, Giovanni(International Atomic Energy Agency Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture) | Dundon, William G.(International Atomic Energy Agency Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture)
Between July and September 2017, samples collected from six unvaccinated chickens in Namibia were shown to be positive for infectious bursal disease virus (IBDV) by RT-PCR. Partial sequence and phylogenetic analysis of the VP1 and VP2 genes from six viruses revealed that they all belong to the very virulent pathotype (Genogroup 3) and are genetically very similar to IBDVs identified in neighbouring Zambia. This is the first molecular characterisation of IBDV in Namibia and has implications on the control and management of the disease in the country.
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