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Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture
2016
Mazurkevych, Anatoliy | Malyuk, Mykola | Bezdieniezhnykh, Natalia | Starodub, Lyubov | Kharkevych, Yuriy | Brusko, Evgen | Gryzińska, Magdalena | Jakubczak, Andrzej
Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture.Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO₂ incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study.Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells.Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.
اظهر المزيد [+] اقل [-]Эффективность использования генов-маркеров EPOR, MUC4 и IGF-2 при повышении продуктивных качеств свиней пород белорусской селекции
2009
Dojlidov, V.A. | Kaspirovich, D.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bykova, M.I. | Mikhajlova, T.I., Selection and Hybrid Centre Zadneprovskij, Vitebsk region (Belarus)
Use desirable alleles allows to raise the reproductive quality of the sows, in particular, amount alive piglets at birth (EPORTT), safety piglets (MUC4CC) meat quality pig (IGF-2QQ) that in turn allows to get the additional income at realization of the saplings
اظهر المزيد [+] اقل [-]