Because caffeine is metabolized by the hepatic P-450 cytochrome oxidase system, clearance of caffeine is an excellent quantitative test of hepatic function in human beings. It is currently used in much the same way that creatinine clearance is used to assess renal function. Caffeine clearance was measured in lactating dairy cows initially to determine the suitability of caffeine clearance as an indicator of hepatic function in cattle. Pharmacokinetic variables of caffeine were studied in 6 adult lactating dairy cows after IV administration of a single dose of caffeine sodium benzoate (2 mg of caffeine/kg of body weight). Caffeine concentration was analyzed by use of an automated enzyme immunoassay. The lower limit of detection of the assay for caffeine in serum was 0.079 micrograms/ml. Serum caffeine concentration-time curves best fit an open two-compartment pharmacokinetic model. Harmonic mean elimination half-life was 3.8 (range, 2.6 to 6.9) hours, and total clearance was 0.118 (range, 0.090 to 0.197) L/kg/h. Milk caffeine concentration was similar to serum concentration 1.5 to 24 hours after caffeine administration. Adverse effects were not observed in cows given caffeine.

Results of studies of the influence of two biological products produced on the basis of sapropel (putrid mud) of the Ganarata lake (Mostovski district, the Republic of Belarus) on quality of legume-grass silage were presented. It was established, that analyzed preparations could be used as the preserving agents for preparation of herbal forages. The preserving action of the preparations was caused by the intensification of lactic bacteria development in silaging mass for the account of humic acids, as well as by the fungicidal action of phenolic and carboxylic fractions of the preparation. Silage preparation in combinations with putrid mud preparations made it possible to lower the losses of a crude protein on 11,3-15,6%, sugar - on 33,3%, carotin - on 20% and to obtain forage with concentration of exchange energy 9,20-9,38 mJ per 1 kg of dry matter. Silage application in combination with biological products rendered positive impact on milking efficiency of cows, the average daily milk yields in the experimental cow groups were on 1,3-1,4 kg or 6,8-7,3% higher. Application of higher quality feeds made it possible to lower the expenses of forages on 0,04 fodder units. Research results proved that the analysed preparations from sapropel could be used as an efficient conservants for silage preparations. They made it possible to raise forage quality at the minimum expenses

The comparative analysis of application of cereal ensilage produced with addition of microbial and enzymatic preserving agents Axphast Gold and Biotrof on fodder digestibility, hematological indexes and cicatrical digestion of cattle rearers was realized in the conditions of the Republic of Belarus. Cattle feeding with the analysed silage rendered positive effect on digestibility of the forage dry matter, which was on 2% higher the control index, organic matter - on 2,4%, nitrogen-free extractive substances - on 2,84%, fat - on 0,58%, protein - on 2,3%, cellulose - on 2,1%. Use in feeding of ensilaged forages from the cereal perennial grasses preserved by the microbial- enzymatic preparation produced by company Biota rendered the positive influence on use of nitrogen, calcium and phosphorus. There was also noted the increasing of the content of volatile fatty acids in paunch of young cattle silage fed with Axphast Gold that testified of more effective utilization of forage

In the conditions of the Republic of Belarus there was studied the efficiency of application of forage chemical preservatives Kormoplyus-1, Kormoplyus-2, and Kormoplyus-3 for grass fodder conservation. The analyzed preservatives contained acetous acid, urotropine and sodium acetate (only in Kormoplus-2). In course of the study there were established experimental batches with the studied sreservative; there was determined a chemical composition of prepared forages; in physiological and scientifical and economic experiment there was analyzed the feeding efficiency of cereal ensilage. Research results showed that application of preservatives Kormoplyus-1 and Kormoplyus-2 2 for preparation of cereal ensilage made it possible to receive a high quality ensilage and to increase the dry matter safety on 4,2-8,7% and protein on 12-9,4% on comparison with storage without preservatives. Also, it made it possible to increase the digestibility of dry matter in cattle diets respectively on 5.5% and3.7%; organic substance - on 5.5% and 4.0%; fibre - on 16,5% and 15,0%, and also to increase nitrogen deposition on 17,4 and 20,8%. Feeding with forages preserved with Kormoplyus-1 and 2 did not render negative influence on a health state and biochemical indices of animal blood.