Free, autogenous, full-thickness skin grafts were applied to 10 dogs; 5 dogs were given an iron chelator, deferoxamine-10% hydroxyethyl pentafraction starch (DEF-HES; 50 mg/kg of body weight, IV), and 5 dogs were given an equal volume of 10% hydroxyethyl pentafraction starch (HES) in 0.9% saline solution (5 ml/kg, IV). All dogs (DEF-HES/HBO- and HES/HBO-treated) were exposed to 60 minutes of hyperbaric oxygen (HBO) at 2 atmospheres absolute pressure twice daily for 10 days, beginning the day of surgery. The percentage of viable graft on day 10 was lower in HES/HBO-treated-dogs (mean +/- SD, 13.3 +/- 21.3%; median, 3.0%) than in DEF-HES/HBO-treated dogs (64.7 +/- 39.2%; 88.3%; P = 0.095, Mann-Whitney two-tailed test). There was a positive correlation between percentage of viable graft (on day 10) and percentage of haired skin on the graft site (on day 28) for all dogs (r = 0.91) and for HES/HBO-treated dogs (r = 0.97). The DEF-HES/HBO-treated dogs had less consistent correlation (r = 0.67). Perivascular aggregates of foamy cells were observed in the superficial and reticular portions of the dermis and in the subcutaneous tissue on both surfaces of the panniculus muscle in the graft sites of DEF-HES/HBO-treated dogs. These cells were also observed in the dermis, but not subcutaneous tissue of the control skin sections, and in some viscera of DEF-HES/HBO-treated dogs. Deferoxamine appears to attenuate the detrimental effect of HBO and HES on survival of free skin grafts. However, clinical use of HBO is not recommended as adjunct treatment for free skin grafts in dogs in the first 10 days after grafting. Administration of DEF-HES is not recommended because it has failed to improve the survival of free skin grafts, and the consequence of the cellular response seen in this study is undetermined.

An experiment was conducted to study the effect of organic trace mineral sources supplementation on egg production of laying hens. It consisted of 8 treatments: one treatment with all trace minerals on inorganic form, one treatment with all minerals on organic form and 6 treatments where only one mineral was from organic origem. The trace minerals were: zinc, manganese, iodine, selenium, copper and iron. Corn and soybean meal basal diet was used. 672 Babcock B300 laying hens with 48 weeks were used. The production parameters analyzed were: Feed intake, egg production, egg mass and feed conversion. Data were summarized for 3 periods of 28 days each and analyzed throught SAS. The results for egg production were not different for the tree periods, except for egg mass witch was lowed in the organic iron treatment in the last period (p<0,05). The experiment results suggest that the supplementation with organic minerals did not improve the egg production for laying hens between 48 - 60 weeks of age, when it was compared with inorganic mineral supplementation. | Um experimento foi realizado para avaliar os efeitos da suplementação dietética de fontes orgânicas de microminerais na produção de ovos de galinhas poedeiras. Foram utilizados 8 tratamentos: um com todos os minerais na forma inorgânica, um com todos na forma orgânica e mais 6 tratamentos com apenas um dos minerais na forma orgânica. Os minerais testados foram: zinco, manganês, iodo, selênio, cobre e ferro. As dietas experimentais foram à base de milho e farelo de soja. Foram utilizadas 672 aves da linhagem ISA Babcock B 300, o período de experimentação foi de 48 a 60 semanas de idade, e os parâmetros de desempenho produtivo avaliados foram: Consumo de ração, produção de ovos, massa de ovos e Conversão Alimentar. Os dados foram analisados em ciclos de postura de 28 dias. A adição dos diferentes minerais isolados ou em combinação, na forma orgânica ou inorgânica, aparentemente não resultou em melhora nas medidas de desempenho, pois à exceção do terceiro período de avaliação, quando obteve-se um menor índice de massa de ovos para o tratamento em que foi empregado o ferro orgânico (p<0,05), não se detectou diferença significativa. Partindo-se da premissa de que as fontes orgânicas de minerais apresentam maior biodisponibilidade, o efeito de seu uso permanece controverso, pois os resultados do experimento sugerem que a suplementação com minerais orgânicos não altera o desempenho da produção de ovos de galinhas entre 48 e 60 semanas de idade, quando comparado com a suplementação inorgânica dos mesmos.

Evaluation of efficiency of chelate forms of trace elements in rations of pregnant sows as a factor of improvement of their production indices and quality of obtained litter. It was determined that introduction into pregnant sows rations of chelate forms of zinc and iron starting from the 84th day of pregnancy and till farrowing led to authentic increasing of milk yield, weight of a litter at birth and at weaning periods, to increasing of live weight of obtained litter, and also proved to be a form of hypothrophics prevention in piglets.