خيارات البحث
النتائج 1 - 3 من 3
Electrofusion of zona-free mouse embryonic cells in electrolytes and their development in vitro
1995
Elsheikh, A.S. (Hokkaido Univ., Sapporo (Japan)) | Takahashi, Y. | Tanaka, H. | Hishinuma, M. | Kanagawa, H.
The influence of increasing the physical electrofusion parameters, direct current (DC) pulse strength, pulse duration, pulse number, alternating current (AC) voltage and alignment time, in electrolytes on the rates of fusion,degeneration and development of zona-free mouse 2-cell embryos were examined. Furthermore, the effects of physiological saline and mannitol as fusion media and various mouse strains were also evaluated. Dulbecco's phosphate-buffered saline (PBS) supplemented with 10% fetal calf serum was used as the main fusion solution. A significant increase in the rate of fusion (P0.05) was obtained by increasing pulse strength from 30 to 300 V/mm. The embryos fused at the pules strengths of 30 to 70 V/mm had significantly higher development rates to blastocysts compared with those fused at 100 to 300 V/mm (P0.05). There were no significant differences in the rates of fusion, degeneration and d development to blastocysts when the pulse duration was increased from 30 to 90 mu-sec. Although fusion rates were increased (P0.05) by increasing the pulse number up to 4, a significant decrease (P0.05) in development to blastocysts was observed when the pulse number was 5. Application of AC voltage prior to the DC pulse tended to increase the fusion rate (89.2-93.8%), compared with fusion with the DC pulse only (75.0%). Prolongation of alignment time from 5 to 15 sec had no effect on the fusion rate. Under the optimum conditions (2 pulses of DC of 7- V/mm, 70 mu-sec pulse duration and AC of 5 V/mm for 5 sec), no significant difference was obtained in the fusion and development rates in different mouse strains, nor were fusion and development rates significantly different among PBS, physiological saline and mannitol solutions (P0.05)
اظهر المزيد [+] اقل [-]Preliminary efficacy trial of cymelarsan in mice artificially infected with Trypanosoma brucei brucei isolated from a dog in Zambia
1995
Syakalima, M. (Hokkaido Univ., Sapporo (Japan)) | Yasuda, J. | Hashimoto, A.
An efficacy trial of Cymelarsan (R) on a Zambian strain of Trypanosoma brucei brucei was done. Twenty-five male mice were infected intraperitoneally with 10(6) of T. b. brucei isolated from a dog. Five groups of 5 mice were treated with 0 (control), 0.25, 0.5, 1.0 and 2.0 mg/kg cymelarsan, respectively. The target was to achieve aparasitaemia for 30 days post-treatment, euthanising those that remained parasitaemic or relapsed before then. The 0.25 and 0.5 mg/kg groups remained parasitaemic although the parasitaemic levels were reduced. The 1.0 mg/kg group had a proportion of aparasitaemic mice. However, all mice in the 2.0 mg/kg group remained aparasitaemic until day 20 when 2 mice relapsed. These results suggested that more than 2.0 mg/kg was required to eliminate this strain
اظهر المزيد [+] اقل [-]Immunohistochemical identification of Cowdria ruminantium in formalin-fixed tissue sections from mice, sheep, cattle and goats
1995
Jardine, J.E. (Pretoria Univ., Onderstepoort (South Africa). Dept. of Pathology) | Vogel, S.W. | Van Kleef, M. | Van der Lugt, J.J