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Bacterial contaminants in extended boar semen and selection of effective antimicrobials
2010
Kim, H.Y., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Byun, J.W., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Shin, D.H., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kim, H.S., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Yoon, H.C., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Park, C.K., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Lee, O.S., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Jung, B.Y., Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Anyang, Republic of Korea
Bacterial contamination is an unavoidable finding of the semen collection process in boar and can lead in deleterious effects on semen quality and longevity if left uncontrolled. The purpose of this study is to identify the bacteria in extended boar semen and to select the effective antimicrobials to control of the contaminants. Of 116 extended boar semen samples submitted from eight AI centers in Korea, 39 (33.6%) samples were positive for bacterial contamination. Among 39 contaminated semen, most of them (84.6%) were contaminated with one or two bacterial species and there was no significant difference between two age groups (less-than or equal to 24 and greater than 24 month old). Stenotrophomonas maltophilia (n=18) was the most predominant bacterium followed by Elizabethkingia meningoseptica (n=12), phingomonas paucimobilis (n=12), Myroides spp. (n=5), Ochrobactrum anthropi (n=3), and so on. Enrofloxacin (72.9%), florfenicol (72.9%), bacitracin (49.2%) and tylosin (49.2%) showed higher sensitivity compared with penicillin (13.6%) or aminoglycosides (6.8%-18.6%). Brucella spp., Leptospira spp., Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycobacterium tuberculosis complex were not detected in semen by PCR.
اظهر المزيد [+] اقل [-]Влияние генотипа хряков белорусской крупной белой и белорусской мясной породы по гену MUC4 на сохранность и продуктивность потомства
2009
Kaspirovich, D.A. | Dojlidov, V.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bykova, M.A. | Mikhajlova, T.A., Selection and Hybrid Centre Zadneprovski (Belarus)
Analysis of influence of boar genotype by MUC4 gene on viability of progeny was realized in the conditions in the Republic of Belarus, as well as the evaluation of possibilities of association of polymorphic variants of gene with fattening and meaty qualities of obtained from the boars progeny were conducted. In course of studies there was analyzed the polymorphism of MUC4 gene at producer boars of studied breeds. There was revealed high occurring frequency of desirable allele MUC4**c and MUC4**cc genotype in all studied populations. Boars of Belarusian Meaty breed with genotypes MUC4**cc by MUC4 gene authentically (P less than 0,01) exceeded boars with genotype MUC4**cg according to piglet viability indexes at weaning period on 5,6%. For the further evaluation of the potential role of MUC4gene in marker selection there was realized determination of relations between genotypes of studied marker and other economic traits of swine (fattening and meaty traits of boar progeny). There was stated the authentic increasing of fattening and meaty traits of stores obtained from boars with homozygous genotype MUC4**cc in comparison with progeny fathers of which in their genotype had mutant allel MUC4**g. For the forecasting and modeling of genotypes resistant to colibacillosis there was presented a scheme of selection boars and sows of different genotypes by MUC4 gene for the increasing of progeny viability. According to the scheme the preferred selection was MUC4**cc x MUC4**cc. This selection was recommended for the self-replacement and creation of nuclear stock
اظهر المزيد [+] اقل [-]Переваримость питательных веществ комбикормов с различным уровнем селена в рационах хряков-производителей
2009
Poznyak, V.V., Vitebsk Meat-Packing Plant (Belarus)
In the physiological experience spent on male pigs-manufacturers, we had been studied influence of various doses of selenium (10 mg, 20 and 30 mg) in structure premix on nutrients. It is established, that increase of level of selenium in mixed fodders for male pigs-manufacturers to 30 mg/kg especially promotes increase in degree of use in their organism of fat to 58,7%; (P less than 0,05), the digested nitrogen to 62,1%, calcium – to 78,3% and phosphorus – to 72,5%
اظهر المزيد [+] اقل [-]Влияние хряков мясных пород канадской селекции на откормочные и мясосальные качества помесного молодняка
2009
Fedorenkova, L.A. | Batkovskaya, T.V. | Yanovich, E.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
Usage of boars of meat breeds Yorkshire, Duroc and Landrace of Canadian selection for crossing them with cross and pure breeds of sows promotes increase of fattening and meat-fat productivity of the obtained hybrids. The best parameters of feeding efficiency the hybrid young growth of combinations (BMхL)хL and (BMхL)хD at which the age of achievement of alive weight of 100 kg and a daily average gain has made 177,2 day (P less than 0,001) and 803 g (P less than 0,001), 179,5 day (P less than 0,001) and 801 g (P less than 0,001), differed at expenses of a forage of 3,38-3,40 fodder units accordingly
اظهر المزيد [+] اقل [-]Влияние натрия тиосульфата на морфологию костного мозга птиц при ассоциированной вакцинации против вирусных болезней
2009
Gromov, I.N. | Prudnikov, V.S., Vitebsk State Academy of Veterinary Medicine (Belarus) | Birman, B.Ya., National Academy of Sciences. Scientific and Practical Center of Animal Breding (Belarus). The S.N. Vyshelesskij Inst. of Experimental Veterinary Medicine
Investigation of morphological changes in bone marrow of young hens in the conditions of application of associated virus vaccines and sodium thiosulphate immunostimulant was realized in the Republic of Belarus. The associated virus vaccine was applied against infectious bursal disease, infectious bronchitis, infectious laryngotracheitis, and Newcastle disease. Research results showed that under the influence of inactivated vaccine against mentioned above diseases there was noted the increasing of quantity of granulocytic cells, there was stated an active hyperplasia of pseudoeosinophil cells. Immunization of young hens with the presented vaccine developed in the Institute of Experimental Veterinary Medicine of the National Academy of Sciences of Belarus promoted a short-term increasing of leucoerythroblastic index. Application of an alternative vaccine developed by Scientific and Production Enterprise Avivak did not render substantial influence on the morphology of bone marrow of chickens. Sodium thiosulphate applied in combination with associated vaccines (in 7% concentration in vaccine) caused the hyperplasia of lymphoid and thrombocyte cells, strengthened the proliferation and maturity of granular leukocytes
اظهر المزيد [+] اقل [-]Полиморфизм гена каппа-казеина (CSN3) в популяции крупного рогатого скота белоруской черно-пестрой породы
2009
Yatsyna, O.A. | Smuneva, V.K., Vitebsk State Academy of Veterinary Medicine (Belarus) | Epishko, T.I., Polesje State Univ., Pinsk (Belarus)
Determination of genetic structure of different population lines of Belarusian white-and-black breed in accordance with locus of kappa-casein gene (CSN3) was realized in the conditions of the Republic of Belarus. In course of study there were used DNA samples of Belarusian white-and-black breed cows (380 samples) and bioprobes of servicing bulls (83 samples). With the help of endonuclease Hindill there were revealed two alleles of kappa-casein (A and B) in DNA preparations. As a result of genetic typing of breeding animals by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods there were revealed three genotypes CSN3**AA, CSN3**AB, and CSN3**BB. The frequency of occurrence of desirable genotype CSN3**BB of Belarusian white-and-black breed of cows depending on breed and linear pertain varied from 2,2% up to 7,69%, for servicing bulls – from 0 up to 11,1% . The majority of homozygotic cows with undesirable cow genotype CSN3**AA belonged to the following lines: of Ruties Edward (2,31646 - 92,3%), Niko (86,0%), Vis Ajdal (933122 - 71,9%), Reflection Sovereign (198998 – 63,9%). The desirable CSN3**BB genotype in most of the cases was stated at Annas-Adema (30587) and Hiltes-Adema (37910) lines. Presence of heterozygote genotype predominated in Annas-Adema (46,1%) and Montvik Chiftein (36,3%) lines. Realized analysis of cow population lines of Danish and Holstein breeds showed that the average frequency of occurrence of CSN3**B allel was 17%, but lower concentration (15%) of CSN3**b allel was typical for the Danish breeds. Frequency of occurrence of CSN3**B allel in Holsten lines was 18%. In was revealed that in 86,6% of cases bulls were the genetic carriers of CSN3**A allel and only 11,4% of animals had the desirable allel CSN3**B in their genotype
اظهر المزيد [+] اقل [-]Влияние импортных хряков породы ландрас на откормочную и мясную продуктивность животных белорусской мясной породы
2008
Shejko, I.P. | Fedorenkova, L.A. | Yanovich, E.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
Influence of 33 imported heads Landrace breed boars of foreign selection on feeding performance and meat productivity of swine of the Belarusian Meaty breed was studied in the conditions of the Republic of Belarus for the enhancement of Belarusian breeds genealogical structure. There was stated the positive influence of Landrace Breed on feeding performance and meat productivity of mixed bred stores obtained after the introductive crossing with sows of Belarusian Meaty breed which was expressed in the predominancy of mixed bred hogs over the purebred ones at the stage of fattening performance test: at the age of reaching 100 kg body weight – on 0,7%; at average daily weight gain - on 1,5%; at feed consumption - on 1,2%. Mixed bred stores also had thinner back fat (on 0,9 mm) and bigger loin (on 0,4 square cm). There was stated the one-sided influence of boars of Landrace Breed on feeding performance and meat productivity indices of mixed breeds. It was established, that hybrids of four lines (Zaboj, Zalet, Zont and Bison) with regard to control groups showed the effect of heterosis at age of achievement of body weight of 100 kg, at a daily average weight gain and at forage consumption in the following rates: 1,5% (Р less than 0,05), 2,8% and 1,5% (Р less than 0,05) for Zaboj line; 2,3%, 4,9% and 2,1% (Р less than 0,01) for Zalet lines; 1,4%, 3% and 1,5% (Р less than 0,05) for Zont lines; 3% (Р less than 0,001), 6,7% and 2,7% for a line of Bison. In meat productivity indices there was stated the in-between character of inheritance which caused the decreasing of fat thickness and increasing of loin eye area at descendants of trial groups of lines: Zaboj, Zalet, Zenit, Zont on 0,88 mm (Р less than 0,05) and 0,6 square cm, 1,7 mm (Р less than 0,01) and 2,1 square cm (Р less than 0,001), 1,3 mm (Р less than 0,01) and 0,9 square cm, 1,4 mm
اظهر المزيد [+] اقل [-]Изменение гематологических показателей у хряков при стимуляции биологически активных точек
2008
Elisejkin, D.V. | Sobolev, D.T. | Levkin, E.A., Vitebsk State Academy of Veterinary Medicine (Belarus)
There was studied the changes of the general protein content, albuminous fractions, antibodies and leukogram in blood of boars at influence by the laser on biologically active points. At laser influence on biologically active points, which are situated in lumber and sacral section spine, is taken place stimulating of body resistance. In the experiences the influence by the laser with various frequency has led to authentic increase in the content of protein in blood at 512 Hz with 75,9 to 81,2 and at 4046 with 75,4 to 80,8 g/l. The percent of eosinocytes in blood which carry out antitoxic function increases - from 2,63 to 4,85% and according to 2,50 to 5,15% at P0,01. Influence by laser radiation has caused number increase limfotsits in blood from 44,38 to 50,13% and according from 45,38 to 49,38% at P0,01. In the research the analysis of the content of antibodies in blood of animals was carried out. It was established that after processing by the laser (P0,05) the content in blood of immunoglobulin A increases at frequency of influence of 512 Hz from 2,2 to 3,2 g/l and at frequency of 4046 Hz from 2,4 to 3,3 g/l. | Цель работы заключалась в изучении изменения содержания общего белка, белковых фракции, иммуноглобулинов и лейкограммы в крови хряков при воздействии лазером на биологически активные точки. При воздействии лазером на биологически активные точки, расположенные в поясничном и крестцовом отделе позвоночника, происходит стимуляция резистентности организма. В проведенных опытах воздействие лазером с различной частотой привело к достоверному увеличению содержания белка в крови при 512 Гц с 75,9 до 81,2 и при 4046 с 75,4 до 80,8 г/л. Процент эозинофилов в крови, которые выполняют антитоксическую функцию, увеличивается с 2,63 до 4,85% и соответственно с 2,50 до 5,15% при Р0,01. Воздействие лазерным излучением вызвало увеличение числа лимфоцитов в крови с 44,38 до 50,13% и соответственно с 45,38 до 49,38% при Р0,01. В исследованиях проведен анализ содержания иммуноглобулинов в крови животных. Установлено, что после обработки лазером достоверно (Р0,05) увеличивается содержание в крови иммуноглобулина А при частоте воздействия 512 Гц с 2,2 до 3,2 г/л и при частоте 4046 Гц с 2,4 до 3,3 г/л.
اظهر المزيد [+] اقل [-]Новые линии в белорусской крупной белой породе свиней
2009
Loban, N.A. | Vasilyuk, O.Ya. | Chernov, A.S. | Asomchik, N.V., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
In course of a long-term selection breeding activities in the conditions of the Republic of Belarus on the basis of Belarusian Large White breed of swine there were created two high-productive lines - Delfina 37755 and Svitanka 3884. Individual selection of animals was realized in accordance with the main indexes of productive ability, development and conformation type. There were selected parent lines, there were used methods of intrastrain breeding and hybrid between lines, mild inbreeding on parental line. Bores and breeding sows were evaluated in accordance with their posterity productivity by means of a control feeding method. Productive indexes for Delfina 37755 line were 11,7 piglets, for Svitanka 3884 line – 11,9 piglets; age of live weight gain of 100 kg – 189 and 182 days, respectively; average daily weight gain – 735 gram and 749 gram; forage expenses – 3,5 and 3,51 fodder units; backfat thickness – 27,2 and 26,2 mm; ham weight – 10,9 and 11,2 k, respectively
اظهر المزيد [+] اقل [-]Влияние генотипа хряков по генам EPOR, MUC4 и ICF-2 на продуктивность потомства
2009
Dojlidov, V.A. | Kaspirovich, D.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bykova, M.I. | Mikhajlova, T.I., Selection and Hybrid Centre Zadneprovski (Belarus)
During the investigation of nuclear DNA of boas of Belarusian meaty breed there was studied the polymorphism of EPOR, MUC4 and IGF-2 genes with the further determination of frequency of alleles and genotypes. Among boars tested for the polymorphism EPOR gene there was stated high percentage of heterozygous samples (EPOR**st): 43,3% for Belarusian meaty breed boars and 66,7% for Belarusian Kripnaya Belaya breed. In course of analysis of the genetic structure by MUC4 gene there was revealed the predominance of animals with the desirable d allele combination (MUC**cc): 60% for Belarusian Kripnaya Belaya breed and 82,2% for Belarusian meaty breed; by IGF-2 gene with homozygotic manifestation of recessive alleles: 46,4% and 63,4%, respectively. In the conditions of the Republic of Belarus there were determined the preferred boar genotypes with EPOR, MUC4 and ICF-2 genes and combinations which made it possible to increase the fattening and meaty qualities of posterity. The research results showed that in the selection breeding schemes it was necessary to consider not only maternal genotype, but also male genotypes giving preference to EPOR**st, MUC4**ss and ICF-2**qq genotypes
اظهر المزيد [+] اقل [-]