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Characterisation and antibiotic resistance of Yersinia enterocolitica from various meat categories, South Africa
2022
Seakamela,Emmanuel M. | Diseko,Letlhogonolo | Malatji,Dikeledi | Makhado,Lavhelesani | Motau,Mmatau | Jambwa,Kudakwashe | Magwedere,Kudakwashe | Ntushelo,Nombasa | Matle,Itumeleng
Yersinia enterocolitica infections impose a significant public health and socioeconomic burden on human population in many countries. The current study investigated the prevalence, antimicrobial resistance profile and molecular diversity of Y. enterocolitica in meat and meat products across various retail outlets in selected provinces of South Africa (SA). In a cross-sectional study, a total of 581 retail meat and meat products were collected from four cities across three provinces of SA. Samples were from beef and pork products, which included 292 raw intact, 167 raw processed, and 122 ready-to-eat (RTE) meats. Samples were analysed using classical microbiological methods for isolation, identification and biotyping of Y. enterocolitica. Conventional polymerase chain reaction (PCR) was performed for confirmation, serotyping, screening of virulence (n = 11) and antimicrobial resistance (n = 18) genes. Phenotypic antimicrobial resistance profiles were determined against 12 antibiotics discs, using disc diffusion method. The overall prevalence of 12% (70/581) was reported across all cities with contamination proportion reported in samples collected from raw intact 15% (43/292), followed by raw processed 11% (18/167) and RTE meats 7% (9/122). All positive isolates were of biotype 1A with 7% (5/70) belonging to bioserotype 1A/O:8. Most of the isolates harboured ymoA, ystB, fepD, ail, fepA, invA and myfA virulence genes. High antimicrobial resistance frequency was observed for ampicillin (94%), cephalothin (83%) and amoxicillin (41%), respectively. Of the 18 tested antimicrobial resistance genes, blaTEM was the most predominant (40%) followed by cmlA (21%). This study reveals the presence of antimicrobial resistant Y. enterocolitica possessing virulent genes of public health importance in products of animal origin, therefore, health monitoring and surveillance of this pathogen is required.
اظهر المزيد [+] اقل [-]Characterisation of Yersinia enterocolitica strains isolated from wildlife in the northwestern Italian Alps
2022
Carella Emanuele | Romano Angelo | Domenis Lorenzo | Robetto Serena | Spedicato Raffaella | Guidetti Cristina | Pitti Monica | Orusa Riccardo
Yersiniosis is a zoonosis causing gastroenteritis, diarrhoea, and occasionally reactive arthritis and septicaemia. Cases are often linked to meat consumption and the most common aetiological agent is the Gram-negative bacilliform Yersinia enterocolitica bacterium. The occurrence of Yersinia spp. among wild animals has mostly been studied in wild boar, but it has seldom been in other species.
اظهر المزيد [+] اقل [-]Molecular Characterization of Y. enterocolitica Isolated from Dairy Environment with Special Reference to the Antimicrobial Activity of Milk Proteins Hydrolysates
2022
Ahmed Ahmed | Hassan Diab | Bassma Hendy | Gaber Batiha | Mohamed Dandrawy | Mona Ahmed El-Zamkan
Yersinia species, especially Yersinia enterocolitica, are considered as the most prevalent milk-borne pathogens. Y. enterocolitica is the causative agent of yersiniosis, a zoonotic disease of growing epidemiological importance with significant consequences for public health. A total of 300 samples out of milk and milk products water and environmental samples were collected from dairy cattle rural house and local dairy shops and vendors to be investigated for presence of Yersinia spp. Isolates were molecularly identified and screened for virulence markers, biofilm production and antimicrobial resistance profile. Finally, the antimicrobial activity of milk proteins hydrolysates against Y. enterocolitica was detected. Yersinia spp. was recovered from 50% of the examined samples. The most prevalent species was Y. enterocolitica (isolated from 21.7 and 30% of milk and its products, and environmental samples, respectively). Detection of virulence genes revealed that 24% of Y. enterocolitica isolates harbored both ail and yst genes. Y. enterocolitica isolates showed high antimicrobial resistance to various antimicrobials. Also, different biofilm phenotypes were produced by these isolates. The most produced biofilm phenotype was moderate (68.9%). The parent proteins (CCP, CWP) and their pepsin hydrolysates (P-CCP and P-CWP) were potentially effective in inhibiting Y. enterocolitica growth and peptides P-CWP exhibited the strongest effect against Y. enterocolitica.
اظهر المزيد [+] اقل [-]Characterisation of Yersinia enterocolitica strains isolated from wildlife in the northwestern Italian Alps
2022
Carella, Emanuele | Romano, Angelo | Domenis, Lorenzo | Robetto, Serena | Spedicato, Raffaella | Guidetti, Cristina | Pitti, Monica | Orusa, Riccardo
Yersiniosis is a zoonosis causing gastroenteritis, diarrhoea, and occasionally reactive arthritis and septicaemia. Cases are often linked to meat consumption and the most common aetiological agent is the Gram-negative bacilliform Yersinia enterocolitica bacterium. The occurrence of Yersinia spp. among wild animals has mostly been studied in wild boar, but it has seldom been in other species. A total of 1,868 faecal samples from animals found dead or hunted were collected between 2015 and 2018 in the Valle d’Aosta region of the northwestern Italian Alps. Alpine ibex faecal samples were collected during a health monitoring program in 2018. Bacteria were isolated via PCR and confirmed as Y. enterocolitica biochemically. Strain antimicrobial susceptibility was tested by Kirby–Bauer disc diffusion, and the presence of virulence factors and antimicrobial resistance genes was investigated using whole-genome sequencing. Yersinia enterocolitica strains of biotype 1A were detected in six faecal samples from red deer (0.93%), roe deer (0.49%) and red foxes (0.7%). Strains found in beech martens (3.57%) and Alpine ibex (2.77%) belonged to biotypes 1B and 5, respectively and harboured the pYPTS01 plasmid that had only been detected in Y. pseudotuberculosis PB1/+. All the isolates were resistant to ampicillin and erythromycin. The biovar 1A strains exhibited different virulence factors and behaved like non-pathogenic commensals. The strain from an Alpine ibex also harboured the self-transmissible pYE854 plasmid that can mobilise itself and the pYPTS01 plasmid to other strains. The beech marten could be considered a sentinel animal for Y. enterocolitica. Phenotypic resistance may account for the ability of all the strains to resist β-lactams.
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