Four colostrum-deprived, 1 day old pigs were orally infected with the porcine enterovirus isolated in Korea. Two of the pigs were treated with dexamethasone (5mg/kg/day) for 3 days. The others as control were infected with porcine enterovirus only. The treated, infected pigs developed ataxia at 3 postinoculation days, neural disorders at 8 postinoculation days, 1 week earlier than those of the controls. Histopathological examination of both the treated and the control pigs revealed non-purulent polioencephalomyelitis consisting of neuronal degeneration, perivascular cuffing, meningeal infiltration, focal and diffuse gliosis. The severity of the histopathological lesions in the treated pigs was more prominent than those of the controls

Microcarrier culture technics were widely used for the massive production of the vertebrate animal cells. In this study, attempt was made to establish the microcarrier cell culture system using Cytodex. Various factors affecting the growth of cells on microcarrier culture were also discussed. In conclusion, the yield of cells in microcarrier culture was several times greater than those in roller bottle and flask culture methods, based on the volume of culture media

The anthelmintic efficacy of Albendazole and Ivermectin against gastrointestinal nematodes, trematodes and cestode was tested in naturally infected Korean native goats. Albendazole was medicated at a dose rate of 10mg/kg of body weight orally and Ivermectin was injected at a dose rate of 0.2mg/kg of body weight subcutaneously. The efficacy was measured by faecal examinations on the day 7th and the day 14th after treatment

In order to know the comparative histological structure of the tongue of some domestic and laboratory animals, the root of the tongue has been investigated by light microscopy. The filiform papillae were mainly distributed in the root of the tongue of most animals and many foliate papillae were also found in the dog and rabbit. The very well-developed mucosa was found in the cattle and in the dog, fowl, rat and mouse was fair. The lamina propria was well developed in the cattle, dog and pig. The muscle layer was well developed in the cattle. The adipose tissue which distributed between the muscle fibers was mainly found in the dog

The present study was carried out to observe the histopathological changes in the lung of A/J, C57BL/6N, DBA/2 and NIH-GP mice intratracheally instillated with benzo(a)pyrene or urethan. In the group of intratracheal instillation with benzo(a)pyrene and charoal powder, squamous cell carcinomas and adenocarcinomas were induced. Tumors were induced in high incidence in the lung of A/J and C57BL/6N mice. Especially, squamous cell carcinomas were induced in high incidence and were well-differentiated in the lung of A/J mice

The purpose of this study was to develop a new method which enables the goat ESR to be used as an effective clinical test. Blood samples were taken from 61 Korean native goats aged above one year old and the effect of tube inclinations, tube bores, tube lengths, environmental temoeratures during tests and packed erythrocyte volumes (PCV) on the ESR were observed. The ESR/hr using capillary hematocrit tube (Micro-Ht-tube) was gradually increased as the tube angle inclined from 90 (vertical) to 15 degrees and the best angle in view of both security and fast sedimentation rate was found to be an angle of 45 degrees

The prevalence of diarrhea caused by enterotoxigenic Escherichia coli was surveyed on 445 calves in 6 farms which were located in the central part of Korea. The incidence of enterotoxigenic Escherichia coli in calves with diarrhea was investigated by detecting the K99 and F41 antigens from the isolated strains of Escherichia coli. The incidence of colibacillosis in calves was 23.3%. Of 238 strains of Escherichia coli isolated from calves with diarrhea, 73 strains (30.6%) were proved possessing the K99 antigen by mannose-resistant hemagglutination (MRHA) using horse red blood cells and 79(33.1%) possessing F41 antigens by MRHA using guinea-pig red blood cells

The present study has been carried out to elucidate the antiestrogenic effects of tamoxifen on RNA and protein synthesis in uteri of immature rats. Immature female Sprague-Dawley rats were allocated into 4 groups and injected with 5 micro g of estradiol-17 beta, 50 micro g of tamoxifen, a combination of both, or vehicle only subcutaneously three times with an interval of 24 hrs respectively. The specific activities of 3H-uridine incorporation into uterine RNA and those of 3H-leucine incorporation into uterine protein were measured before and 1, 3, 6, 12, 24, 48 and 72 hrs after the above treatments

The purpose of this study was the examination for presence of K99 antigen (K99), enterotoxigenicity, 0-groups, colicin and antibiotic susceptibility among E. coli isolated from calves and cows. A total of 49 (18.7%) among 262 strains, isolated from 30 (26.5) out of 113 calves and cows, possesed K99, and thirty three of 49 K99+ strains produced ST. Of the strains of diarrheal calf origin which less than 15 days old, a high correlation was observed between enterotoxigenic ability and K99: 92.3% of the K99+ strains produced heat stable enterotoxin (ST)

The present study was undertaken to investigate the development of early embryos, the implantation rate in immature rats superovulated by transplantation of a pituitary gland. Thirty-day-old male rats were performed orchidectomy 15 days before removal of each pituitary orchidectomy 15 days before removal of each pituitary gland. A pituitary gland was transplanted under the right kidney capsule of 28-day-old female rat between 0700 and 1000h on the starting day of experiment which was designated as Day -2. Control rats were administered 4 IU PMSG at 1000 on day -2. Some of rats used to experiment were sacrificed to examine the changes of ovarian and uterine weights on days -2, -1, 0, 1, 2, 3 and 5. Other rats were sacrificed to recover the ova and observe the ovaries on Days 1, 2, 3 and 5. The implantation sites were counted on Days 8