In this study five serotypes of E. coli were isolated from chickens and identified as O1, O2, O6, O78 and O126 out of 33 isolates derived from a total of 60 samples. SDS-PAGE revealed that four proteins were characteristic and shared in all these serotypes at the molecular weight of 21, 30, 55 and 74 kDa of which 55 and 74 kDa proteins were fully reacted with the antisera against E. coli in the western blot. Other proteins are present but varied from one serotype to another.

A total number of 28 buffaloes aged between 5-7years, body weight ranged between 400-500 kg, and belonged to private farms at Assiut Governorate constituted the materials of this study. Twenty of them showed the classical signs of hypophosphataemia while the other eight buffaloes were proved to be healthy by both clinical and laboratory investigations, used as control group. Biochemical analysis of blood sera showed a highly significant hypophosphataemia, hypocalcaemia and hypomagnesaemia in diseased buffaloes either pre or post treatment when compared with the healthy control ones. Meanwhile, the examination of blood showed marked decreases in erythrocytic count, haemoglobin concentration and packed cell volume in diseased animals. All parameters improved in affected buffaloes after 10 days of treatment. The chemical analysis of agronomical samples of soil and drinking water were done. The statistical analyses between the studied parameters were carried out in buffaloes before and after treatment.

This study was conducted to investigate the efficacy of the Newcastle disease (ND) live vaccines from different commercial sources used in different programs for vaccination of chicks having maternal antibodies against Mycoplasma infection. The immune response was estimated using HI and challenge tests. The effect on the chicken performance was estimated by the detection of the body weight gain. The role of vaccines in stimulating respiratory bacterial stress was pointed out by the lesion scores. Birds vaccinated with live vaccines from source (2) showed higher HI titers than those vaccinated with vaccine from source (1) and birds received the 2nd vaccination from heterologous source showed lower titers than those received from homologous source. Results of the challenge test indicated that birds vaccinated with live ND vaccines from one source and those vaccinated with Hitchner B1 at 33-days of age instead of La Sota showed 100% protection rate as compared with 95 % followed by vaccination with heterologous La Sota. Birds received Hitchner B1 of vaccine source (1) showed only 85% protection rate. All vaccinated chicken groups showed feed conversion rates lower than the non-vaccinated control one. Groups received Hitchner B1 as a 3rd dose of the vaccine showed higher rates than those vaccinated with La Sota. Results of the lesion score for chronic respiratory disease (CRD) in vaccinated groups with different regimes revealed that birds received ND vaccinal strains from source (1) having higher scores than those received ND vaccines from the 2nd source. Administration of Hitchner B1 vaccine at 33-days of age showed lower scores. Hitchner B1 can be recommended in vaccination of chickens derived from Mycoplasma infected hens.

Two experiments were carried out to evaluate the efficacy of the administration of active dry yeast and/or lactobacillus preparation (AVI-BAC), either before or after the infection with antibiotic resistant field strain of Escherichia coli O127 (E. coli O127) in controlling the severity of infection in quail chicks. The quail chicks of the different experimental groups were infected orally for two successive days with 3x107 CFU of E. coli O-127 as an individual dose. The used field strain proved to be highly pathogenic for quails. Probiotics were supplemented in the drinking water for the different treatment groups at a dose level of 0.5 gm/L. The results revealed that the inclusion of lactobacilli or active dry yeast before E. coli infection has been highly effective in reducing mortality rate, organ invasion and the number of E. coli positive quail chicks. In addition, it decreased the severity of macroscopic and microscopic lesions in different organs in the probiotic treated groups as compared to the infected controls. Lactobacilli preparations were more efficient in controlling the severity of the infection. On the other hand, the administration of yeast and /or lactobacilli after inducing E. coli infection reduced the mortality rate and the severity of lesion score in different organs but probiotics failed to protect quail chicks against the infection. It has been proved that the two probiotics have synergistic effect in controlling collibacillosis in quails.

A. N. Al Khalaf helminthes , Egret , Egretta alba , Saudi ArabiaExamination of the alimentary tracts of 9 Great Egret (Egretta alba) found freshly died in their inland places around Al-Qassim area during migration season in September and October revealed infection by 6 species of helminths. The extracted parasites were include two cestode worms, three nematodes and one acanthocephala. They were identified as Uniciuna species, Choano-taenia, Subulura species, Contrac-aecum species, Syphacia species and Polymorphus species. None of any examined carcass appears free from infection. The rate of distribution of the parasite in the examined birds was varied between 66.6%, 11.1%, 44.4%, 44.4%, 66.6% and 2.2 % for the previous parasites respectively. The rate of Parasite burden for each species was moderate to low, as it was 3.4, 2.0, 2.65, 3.25, 5.16 and 4.5 from the previous parasites respectively. None of these parasites was published previously in Saudi Arabia as infect this type of Egret. Their morphological characters and their distribution were described in detail

Killing activity of fresh Oreochromis niloticus serum was used as an indicator of the natural immunity. Serum killing activity was tested in temperature stressed fish exposed to 32 ±1°C to different Pseudomonas fluorescens strains. As a clinical estimate of natural immunity and disease resistance, experimental challenge with P. fluorescens strains was conducted. The results cleared that serum killing activity at 32±1°C was almost stopped where fish got infected after bacterial challenge. On the other hand, control fish group kept at 23± 1°C developed serum killing activity and resistance to the acquisition of infection after challenge. A correlation was found between resistance activities of P. fluorescens strains to serum killing and their corresponding courses of infection after bacterial challenge. However, the results of experimental challenge conducted at 32±1°C suggested that the immunocompromising state resulted from the stress of inability to withstand high water temperature is exclusively the critical aspect for the induction of infection.

The Anti-inflammatory, antipyretic and analgesic effect of Achillea millefolium and Salix were investigated in rats and mice. The extracts of Achillea millefolium exerted significant antiinflammatory, antipyretic and analgesic effects. Also potentiated the sleeping time of thiopental sodium in mice. The doses used were 10.375 and 20.75 (watery extract) and 9.5 and 19 mg/100 gm b.wt. (ethanolic extract). Salix produced the same effects at doses of 0.0825 and 0.165 (watery extract) and 0.0850 and 0.170 mg/100 gm b.wt. (ethanolic extract). The extracts of both plants have an anti-inflammatory, antipyretic and analgesic effect

The efficacy of oil extract of the seeds of Loranthus europeus on wound healing was investigated. After the preparation of the oil extract, the oil ointment of L. europeus was prepared. A preliminary biochemical analysis were carried out to find out the chemical contents of L. europeus oil extract. The study involved 18 male rabbits, they were divided equally and randomly into 3 groups depending on post wounding biopsy: p(3'd,7Ih and l4‘hday post wounding). Two full thickness cut wounds were done on the both sides of shoulder regions( the left wound as control and the left as treated wound) of each group of animals. The treated and control wounds were treated continuously with (0.5mg) oil ointment and Vaseline base respectively, for 14”‘ day twice a day. All wounds were evaluate macroscopically which included "measurement of contraction rate, daily wound contraction, hyperemia, exudation and scab formation." and microscopically for "neutrophil, macrophage infiltration, re-epithelization, fibroblast proliferation with collagen production and new blood capillary formation". A Both macroscopic and microscopic results showed the efficacy of L europeus seeds in promoting the healing process significantly as compared with control wounds (P

This study was achieved on 75 cross breed cows ( Jarubi X fresian) , The PH of vagina and its secretion was measured for all cows during the different oesturs phases ( proestrus estrus and l:uring different stages of pregnancy ) PH was measured by using PH paper. Results revealed that aginal PH of cows tends to be alkaline during proestrus; estrus and during luteal phase but in case of pregnancy the vaginal PH becomes acidic on the contrary to that of ewes. Results also indicated that there is no significant effect of neither cows age nor pregnancy frequency on PH Values of cows vagina and its secretion; results also showed that vaginal PH and its secretion had no signification effect on correlation between them. Results cleared that it is possible to diagnose pregnancy by means of PH values during this stages of pregnancy in an accuracy of 100% in pregnant cows confirmed by rectal

The study included the evaluation of tire haematological effects resulting from intramuscular injection of ( 1 mg/kg ) of Li! ocaine (group A) , Diclofenac (group B) and Lidocaine — Diclofenac mixture (group in experimental mice , as a result there was a significant decrease in haemoglobin dpncentration and reached it's lowest value in group C ( 7.04 gm/100ml ) . There was also a significant increase in both total WBCS cpunt and eosinophils number reached it's highest number in group C ( 9.88 X 103/mm’ , ll.2 % ) respectively , while the highest number basophils appeared in group B and reached ( 8 % ) , the same group showed a significant decrease in neutrophils number which reached to ( 17.6 % ) . the a granulocytes ( lymphocytes and monocytes ) showed no changes in number in all groups .