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Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae النص الكامل
2022
Sánchez Antonio | Contreras Antonio | Sánchez-Corral María L. | Martínez-Nista Carmen | Collado Soledad | Sáez José L. | Minguez Olga | Fe Christian de la
Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae النص الكامل
2022
Sánchez Antonio | Contreras Antonio | Sánchez-Corral María L. | Martínez-Nista Carmen | Collado Soledad | Sáez José L. | Minguez Olga | Fe Christian de la
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test.
اظهر المزيد [+] اقل [-]Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae النص الكامل
2022
Sánchez López, Antonio | Contreras de Vera, Antonio | Sánchez-Corral, María L. | Martínez-Nista, Carmen | Collado, Soledad | Sáez, José L. | Minguez, Olga | de la Fe Rodríguez, Christian | Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
© 2022 A. Sánchez et al. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Veterinary Research. To access the final edited and published work see https://doi.org/10.2478/jvetres-2022-0010 | Introduction: Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test. Material and Methods: This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests. Results: All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93). Conclusion: The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs.
اظهر المزيد [+] اقل [-]Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae النص الكامل
2022
Sánchez, Antonio | Contreras Martín, Antonio | Sánchez-Corral, María L. | Martínez-Nista, Carmen | Collado, Soledad | Sáez, José L. | Minguez, Olga | Fe, Christian de la
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test. This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests. All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93). The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs. The agreements show that any of these ELISA tests could be equally well used for diagnosis in programmes against CA.
اظهر المزيد [+] اقل [-]Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China النص الكامل
2022
Xifeng Wang | Kai Zhang | Guowu Zhang | Zhiyuan Li | Yunxia Shang | Chengcheng Ning | Chunhui Ji | Jun Qiao | Qingling Meng | Xuepeng Cai
Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China النص الكامل
2022
Xifeng Wang | Kai Zhang | Guowu Zhang | Zhiyuan Li | Yunxia Shang | Chengcheng Ning | Chunhui Ji | Jun Qiao | Qingling Meng | Xuepeng Cai
Fasciola hepatica is a trematode infecting ruminants worldwide and occasionally affecting other animal species, including humans. It causes significant economic losses. Geographic distribution and patterns of infection must be considered before control and management measures are developed for this parasite. DNA molecular markers are useful for the identification of flukes and elucidation of their genetic evolution. Therefore, the population structure of F. hepatica was studied using this method in sheep in Xinjiang, China.
اظهر المزيد [+] اقل [-]Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China النص الكامل
2022
Xifeng, Wang | Kai, Zhang | Guowu, Zhang | Zhiyuan, Li | Yunxia, Shang | Chengcheng, Ning | Chunhui, Ji | Jun, Qiao | Qingling, Meng | Xuepeng, Cai
Fasciola hepatica is a trematode infecting ruminants worldwide and occasionally affecting other animal species, including humans. It causes significant economic losses. Geographic distribution and patterns of infection must be considered before control and management measures are developed for this parasite. DNA molecular markers are useful for the identification of flukes and elucidation of their genetic evolution. Therefore, the population structure of F. hepatica was studied using this method in sheep in Xinjiang, China. The molecular characteristics, genetic relationships within the population and dispersal patterns of F. hepatica isolates were analysed based on the cox1 and nad1 genes. The population structure of F. hepatica from three regions of Xinjiang was explored and a neutrality test was conducted. The cox1 and nad1 genes have 21 and 42 variable sites, respectively, which can be classified into 34 and 33 haplotypes. Median-joining network and phylogenetic tree analyses showed that there was no significant variation in F. hepatica isolates between the three geographical regions. Analysis of variance revealed that the genetic variation of F. hepatica was mainly present within the populations. The neutrality test indicated that the populations were relatively stable but the Hami population may have undergone short-term expansion. This study revealed for the first time the molecular characteristics, genetic diversity and dispersal patterns of F. hepatica isolates from sheep in Xinjiang, thus providing new insights into the genetic variation and haplotype diversity of F. hepatica from indigenous sheep.
اظهر المزيد [+] اقل [-]Genetic and pathogenic characterisation of a virulent Akabane virus isolated from goats in Yunnan, China النص الكامل
2022
Gao Hua-Feng | Wang Jin-Ping | Yang Zhen-Xing | Xie Jia-Rui | He Yu-Wen | Hong Qiong-Hua | Xin Ai-Guo
Genetic and pathogenic characterisation of a virulent Akabane virus isolated from goats in Yunnan, China النص الكامل
2022
Gao Hua-Feng | Wang Jin-Ping | Yang Zhen-Xing | Xie Jia-Rui | He Yu-Wen | Hong Qiong-Hua | Xin Ai-Guo
Akabane virus (AKAV) has been detected in a variety of host species in China, but there are only limited records of its occurrence in goats. However, more attention needs to be paid to understanding the diversity of viruses in this species. The aim of the study was to explore the genotype characteristics and variation trend of AKAV and their relationship with virulence in Yunnan, China.
اظهر المزيد [+] اقل [-]Genetic and pathogenic characterisation of a virulent Akabane virus isolated from goats in Yunnan, China النص الكامل
2022
Gao, Hua-Feng | Wang, Jin-Ping | Yang, Zhen-Xing | Xie, Jia-Rui | He, Yu-Wen | Hong, Qiong-Hua | Xin, Ai-Guo
Akabane virus (AKAV) has been detected in a variety of host species in China, but there are only limited records of its occurrence in goats. However, more attention needs to be paid to understanding the diversity of viruses in this species. The aim of the study was to explore the genotype characteristics and variation trend of AKAV and their relationship with virulence in Yunnan, China. Blood samples were collected from goats during routine surveillance of goat diseases in Yunnan province in 2019. The AKAV CX-01 strain was isolated using BHK-21 cells. To understand pathogenicity, the virus was intraperitoneally (IP) and intracerebrally (IC) inoculated into suckling mice and tissue samples were subsequently analysed histopathologically and immunohistochemically. Akabane virus CX-01 strain induced encephalitis and impairment of the central nervous system with fatal consequences. Phylogenetic analysis based on the ORF sequences of the small segments indicated that the AKAV isolate used was most closely related to the GD18134/2018 Chinese midge and bovine NM BS/1strains, while phylogenetic analysis based on the medium segments showed a close relationship between CX-01 and the Chinese GLXCH01 strain. The CX-01 isolate was related to AKAV genogroup Ia and probably originated from a recombination of different strains.
اظهر المزيد [+] اقل [-]Field trial of antibody response to inactivated bacterial vaccine in young Holstein calves: influence of animal health status النص الكامل
2022
Mori Kazusa | Otomaru Konosuke | Kato Toshihide | Yokota Osamu | Ohtsuka Hiromichi
Bovine respiratory disease (BRD) is one of the primary causes of death in young calves. Vaccination against infection by the common bacteria causing BRD is possible; however, the physical condition of the young calves that enables antibody production when stimulated by early immunisation remains to be elucidated.
اظهر المزيد [+] اقل [-]Influence of natural and climatic conditions on the distribution and forms of contagious agalactia in sheep in Bessarabia, Ukraine النص الكامل
2022
Volodymyrovych Bohach Mykola | Igorovych Bolotin Vitaliy | Mykolaivych Bohach Denys | Piven Olha Tarasivna | Pyvovarova Iryna Victorivna
Contagious agalactia of ruminants is an endemic disease caused by Mycoplasma agalactiae in flicting significant losses on farms in deaths and forced slaughter of sick animals, abortions, births of sick young animals, and reduced milk and wool production. The aim of the study was to determine the influence of hydrometeorological conditions on the distribution and forms of contagious agalactia in sheep in Bessarabia, Ukraine.
اظهر المزيد [+] اقل [-]Antimicrobial resistance and virulence genes in staphylococci isolated from aviary capercaillies and free-living birds in south-eastern Poland النص الكامل
2022
Sulikowska Magdalena | Marek Agnieszka | Jarosz Łukasz | Stępień-Pyśniak Dagmara | Urban-Chmiel Renata
The current study characterises Staphylococcus bacteria recovered from dead free-living birds and captive capercaillies kept in south-eastern Poland. The results provide novel information about the antimicrobial resistance phenotype/genotype and the virulence profile of these bacteria.
اظهر المزيد [+] اقل [-]Prevalence of virulence genes among Escherichia coli strains isolated from food and carcass swabs of different animal origins in Croatia النص الكامل
2022
Stojević Dora | Humski Andrea | Mikulić Marina | Dobranić Vesna | Reil Irena | Duvnjak Sanja | Benić Miroslav | Beck Relja | Cvetnić Željko
Escherichia coli is present in the normal intestinal flora but some strains can cause intestinal and extraintestinal diseases, and research on its presence in food of animal origin is in the interests of public health. This study was designed to characterise E. coli strains according to their origin, their carriage of virulence genes specific for certain pathogroups, and phylogenetic group affiliation.
اظهر المزيد [+] اقل [-]Detection of a new emerging strain of rabbit haemorrhagic disease virus 2 (GI.2) in China النص الكامل
2022
Chen Wanting | Tu Teng | Luo Yan | Yang Zexiao | Yao Xueping | Wu Xulong | Wang Yin
In May 2020, an outbreak of rabbit haemorrhagic disease 2 (RHD2) caused by the rabbit haemorrhagic disease virus 2 (RHDV2, GI.2) occurred in Sichuan, China. The acute onset and short disease course resulted in rabbit mortality as high as 42.86%. Currently, basic research on the aetiology and genetic characteristics of GI.2 is lacking in China.
اظهر المزيد [+] اقل [-]Seroprevalence and epidemiological risk factors for Kasba virus among sheep and goats in South Korea: a nationwide retrospective study النص الكامل
2022
Hwang Jeong-Min | Ga Yun Ji | Yeh Jung-Yong
Kasba virus belongs to the Palyam serogroup of the Orbivirus genus and Reoviridae family. Kasba virus is the causative agent of many reproductive disorders in infected animals, which result in considerable economic losses, mainly in the cattle industry. The epidemiology of Kasba virus infection is poorly defined and remains unclear in South Korea.
اظهر المزيد [+] اقل [-]Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages النص الكامل
2022
Urban-Chmiel Renata | Nowaczek Anna | Chmiel Paulina | Wawrzykowski Jacek | Pyzik Ewelina | Marek Agnieszka | Stępien-Pyśniak Dagmara | Alomari Mohammed M.M. | Dec Marta
The widespread occurrence of drug-resistant bacteria has increased interest in alternatives to antibiotics for combatting bacterial infections, among which bacteriophages play an important role. The ability of phage proteins to induce an anti-phage immune response can significantly limit the effectiveness of treatment, which was the basis for the study described in this article. The aim of the study was to assess the effects of bacteriophages on the induction of an anti-phage humoral response in calves.
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