خيارات البحث
النتائج 1541 - 1550 من 22,132
Ultrastructure of virus particles in the liver of piglets infected with porcine enterovirus serotype 3.
1992
Shin T.K | Lee C.S. | Huh M.D.
Spontaneous lingual papillomas in fischer 344 rats.
1992
Kang B.H. | Lim C.H.
Isolation of infectious pancreatic necrosis virus from rainbow trout in Korea.
1991
Lyoo Y.S. | Chang C.H. | Jeon Y.H. | Lee J.O. | Rhee J.C.
Isolation of Getah virus from racehorse in Korea.
1991
Lyoo Y.S. | Chang C.H. | Rhee J.C. | Kim Y.J. | Lee S.Y.
Characteristics and application of monoclonal antibody to progesterone, 2; Development of progesterone enzyme-linked immunosorbent assay (ELISA).
1991
Kang C.B. | Kim J.S.
Fine structure of Theileria sergenti merozoite in Korean native cattle.
1990
Baek B.K. | Kim B.S. | Lee H.I.
Light microscopic observations on the in vitro effects of praziquantel on Heterophyopsis continua.
1990
Woo H.C. | Suh M.D. | Hong S.J.
Avidin-biotin complex for immunohistochemical diagnosis of Aujeszky's disease and hog cholera.
1990
Kim S.B. | Sur J.H. | Moon U.G.
Immunohistochemical identification of Newcastle disease virus with indirect immunoperoxidase technique.
1990
Nho W.G. | Sur J.H. | Kim S.B.
Study on improvement of viability of mouse embryos after bisection.
1989
Lee H.J. | Kim T.S. | Choe S.Y. | Park H.S. | Park C.S.
Demi-embryos were successfully produced by bisection of ICR mouse embryos at preimplantation stages. They were microsurgically bisected using a microsurgical blade attached to a micromanipulator after pretreatment with 0.5 % pronase in PBS for two minutes or not. Embryos with softened zona pellucida were more easily bisected and less damaged than intact embryos. The highest success rate in bisection has been achieved by selecting blastocysts (94.1 % in success rate with intact blastocysts and 100 % in success rate with zona softened blastocysts). Demi-embryos without zona pellucida were cultured in D-PBS or M-16 medium at 37deg C, 5 % CO2 in air for 72 hours for 2-cell stage embryos, 48 hours for 4-to 8-cell stage embryos, 24 hours for morula stage embryos and 6-12 hours for blastocyst stage embryos. For the in vitro culture of 2-cell stage embryos, 100 micro M 2Na-EDTA was added to the media. M-16 medium was better for the in vitro development of mouse embryos than PBS, and PBS is not considered to be suitable for long-term culture of embryos, especially at early stage of cleavage. In M-16 medium, developing rate of demi-embryos of which pair underwent development to form eublastocysts was 15.8 % at 2-cell stage, 16.8 % at 4-cell stage, 38 % at 8-cell stage, 89.6 % at morula stage and 94.4 % at blastocyst stage, respectively. The more rapid and efficient production of demi-embryos and higher viability after bisection can be expected by softening zona pellucida with pronase and by selecting morulae or blastocysts rather than embryos at early stage of cleavage.
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