A survey was undertaken to screen and evaluate the presence of organochlorine pesticide residue in poultry products. Both commercial and desi chicken eggs and meat samples were collected from Namakkal area of Tamil Nadu and analyzed by thin layer chromatography and confirmed by gas chromatography- mass spectrometry. Total Endosulfan in commercial chicken egg and in broiler thigh and breast muscle exceeded the MRL of Codex alimentarius. The occurrence was 50 percent with regards to broiler meat and 33.33 percent with regards to commercial egg. Desi chicken adipose tissue had a high incidence of endosulfan residue (58.33 per cent) with the levels of 28.57 ± 2.85 ppb. But the detected mean values were below the permissible MRL. DDT (Dichlorodiphenyltrichloroethane) was detected in broiler thigh and breast meat. The values obtained were below the MRL of Codex alimentarius and EU standards. BHC (Benzene hexa chloride) was detected in broiler liver, thigh and breast meat while the per cent incidence was higher in liver (33.33). The detected mean values were below the maximum permissible level of Codex alimentarius and EU standards. Both desi and broiler egg and meat analyzed were negative for aldrin, dieldrin and endrin residues which indicate that there may be these pesticides are not used in the geographical area from where samples were obtained. The findings of this study indicates that the presence of organochlorine pesticide residues, though at low level of incidence, still continues to be a threat for poultry products, hence it is essential to have a constant screening and monitoring of these compounds in feedstuff and feed at feed mills and mixing plants so as to prevent contamination in the eggs and meat.

We estimated the incidence of clinical mastitis (CM) and antimicrobial agent (AMA) usage for its treatment in an organized dairy farm over a period of six months. The incidence of mastitis at different stages of lactation was also quantified. The incidence of mastitis was higher in Sahiwal cows (9.52%) compared to crossbred cows (3.61%). Among different AMA usage, enrofloxacin (22%), ampicilin with cloxacillin (18.57%), gentamicin (18.29) and ceftriaxone (12.57%) drugs were most commonly used against CM cases. Penicillin group and their combinations (29.43%) was found to be the most commonly used AMA for CM followed by fluoroquinolone (22%), aminoglycosides groups (21.43%) and cephalosporins group (16.86%) of drugs. Tetracyclines (9.14%) and chloramphenicol (1.14%) were least choice as evidenced by less frequent usage. From the findings of the study it may be concluded that the incidence of mastitis was high in Sahiwal cows milked with machine milking system and mastitis animals needed an average of five days treatment for clinical cure. Further, â-lactam group of antimicrobial drugs were predominantly used in mastitis treatment, despite entry of new AMA in the market.

Antimicrobials used in poultry production have the potential to bioaccumulate in poultry feathers but available data are scarce. Although feathers were processed and introduced as a protein source in animal feed, withdrawal periods were not established. Hence, an experimental trial was conducted to evaluate the residuol profile of enrofloxacin and its primary metabolite ciprofloxacin in featherof broiler chicken. Thirty six one-day old broiler chicks were randomly divided into control (6 nos) and treatment group (30 nos). Treatment group was administered with enrofloxacin at recommended therapeutic dose 10 mg Kg , through drinking water for five consecutive days from 43rd  to 47th -1 day of age, whereas control group received non-medicated water. Six birds from treatment group were sacrificed ethically and feather samples were collected at different time points during the withdrawal period at 48 hours interval on day 1, 3, 5, 7 and 9 post treatment. Control birds were sacrificed on day 9 post treatment. Enrofloxacin and ciprofloxacin residues in feather were analysed by a validated High Performance Thin Layer Chromatography Fluorescent Densitometry method. The present study revealed that enrofloxacin persisted in feather throughout the study period, where as its metabolite ciprofloxacincould be detected until 7th day post treatment and on 9th day post treatment it was below detection limit. The interesting finding is the high level of enrofloxacin achieved in feathers during the withdrawal period. Drug withdrawal times, based on the concentrations of antimicrobial residues in edible chicken meat, were not adequate to reduce antimicrobial residues in chicken feathers. As the feathers are used as a protein source to supplement feed for different food animal species (bovines, pigs, Salmons, trouts) they must be considered potential reservoirs of chemical residues that can reach man through the food chain; hence, the present study warrantsestablishment of withdrawal period for feather similar to that of edible tissues. 

In - vivo evaluation of  the  efficacy of test anti - coccidial efficacy of salinomycin and amprolium carried out in two different trials in 228 commercial chicken revealed 100% efficacy against mixed eimeria sp on comparative evaluation with standed in feed anti - coccidials.  There was marked reduction in the oocyst output with increased body weight gain and improved feed conversation efficiency in the experimental study.

The present study was carried out in eight highly duck populated districts comprised of four clusters of Tamil Nadu. From each of the clusters, 40 duck farmers were selected randomly to study their socio-economic status. Using a pre-tested, structured schedule, data were collected through face to face interview method. The results of the study revealed that 50.60 per cent of the farmers belonged to old age group (Above 45 years). Most of the farmers were married (92.50 %) and majority of them were found to be illiterate (48.80 %). Most of the farmers found to be rearing ducks as their primary occupation (84.37 %). Highest proportion of farmers (43.75 %) had duck farming experience of 37 – 55 years. The risk-taking abilities of the duck farmers were at medium level (51.50 %). Nearly one-half (48.75 %) of the respondents had contact with input dealers followed by 33.75 per cent had contact with progressive farmers for obtaining information’s related to duck farming. 

Three cases of acrochordon were diagnosed by gross, cytological and histopathological examinations. Each case was an unique presentation, in which patient exhibited a soft, pendulous mass on the ventral thorax, right thigh and a small, firm growth on the tail. The affected animals were a six-year-old Rottweiler bitch, a four-year-old Labrador bitch and a six-year-old Labrador dog. All the animals appeared clinically normal. On gross examination, the growths were pendulous, soft and ulcerated. The cut surface appeared creamy white in colour. Cytological analysis of impression smears revealed individual to multiple spindle cells. Histopathological examination revealed a redundant dermal fibrous tissue covered by a hyperplastic epidermis. The stromal component appeared more prominent in these cases. Based on the gross and histopathological features, the cases were diagnosed as acrochordon.

A case was diagnosed as canine multicentric lymphoma based on clinical presentation, FNAC and ultrasonography and was treated with CHOP-19 protocol and remission was observed on 9th week but the protocol was continued up to 19th week and no signs of relapse was noticed. The animal was monitored every month for month after the treatment (19 weeks) animal showed no signs of reoccurrence up to 7 months. Multicentric lymphoma is a disease that the general practitioner can manage; it does not require referral to a specialized practice.

The current study utilized 20 postpartum anestrus buffaloes maintained at an organized dairy farm in Vuyyuru, Krishna district to compare the metabolic (glucose), mineral (calcium) and anti-oxidant (superoxide dismutase) levels in ovsynch-48 (n=10, G48) and ovsynch-56 (n=10, G56) postpartum anestrus graded Murrah buffaloes. G48 group of buffaloes received 20 µg of GnRH on Day 0, followed by 500 µg PGF2 on Day 7, a final dose of 20 µg of GnRH was given 48 hours after PGF2α on Day 9. Whereas, G56 group of buffaloes received 20 µg of GnRH on Day 0, followed by 500 µg of PGF2α on Day 7, a final dose of 20 µg GnRH was given 56 hours after PGF2α on Day 9. In both groups, FTAI was performed at 16-21 hours interval after observing estrus. Results revealed that on different days of treatment, serum glucose and calcium levels did not differ significantly (P>0.05) in both treatment groups; however, Superoxide dismutase levels varied significantly (P<0.05) on different days of treatment in both groups. The results of the study parameters could play pivotal role in reproductive function and may provide insights into the underlying mechanism of anestrus.

The present study was aimed to assess the efficacy of oocyte retrieval through transvaginal ovum pick-up (OPU) in Ongole (Bos indicus) cows. Cows (n=18) were divided in to two equal groups; cows in group 1 cows were subjected to two OPU sessions (OPU1 and OPU2) at 96 h interval irrespective of the stage of estrus cycle. Cows of group 2 were subjected to FSH pre-stimulation before OPU 1 followed by OPU 2, 96h later. Thus, a total of 36 OPU sessions were performed on 18 animals.  The number of follicles available for aspiration (17.89 ± 1.78 vs 27.06 ± 1.75), number of medium (4 – < 8 mm; 4.11 ± 0.69 vs 16.00 ± 1.76) and large follicles (≥ 8mm; 1.06 ± 0.23 vs 6.33 ± 0.79), follicles aspirated (11.95 ± 1.42 vs 17.45 ± 2.07), COCs recovered (5.72 ± 0.78 vs 10.06 ± 1.78), and viable COCs collected (4.23 ± 0.67vs 8.34 ± 1.79) were significantly higher in group 2 than in group 1.The mean number of follicles aspirated, the mean oocyte recovery and the viable oocytes collected were significantly higher at OPU 1 in both the groups than at OPU 2. It was concluded that pre-treatment with FSH increased the OPU efficiency in terms of oocyte yield and viable oocytes collected. OPU 2 performed at shorter interval after OPU 1 is not advantageous due to limited number of follicles available for aspiration at OPU 2 and consequently, reduced oocyte recovery.

The present study was aimed to investigate the presence of Escherichia coli (E.coli) in raw chicken meat samples collected from retail shops, as well as the biofilm-forming ability of field isolates, and to characterize different adhesion genes. Out of 20 chicken meat samples, 17 (85%) were positive for E. coli. Fifteen E. coli strains were characterized by PCR using the 16S rDNA primers and all the isolates were positive which confirmed that all the isolates were E.coli. Out of the 15 confirmed E.coli field isolates which were subjected to biofilm-forming assay, 46% of them were found to be strong biofilm producers. While all the isolates were screened for the presence of adhesion genes viz. luxS, csgA, fimH, fimA, and papC, the adhesion gene luxS was detected in all the strains (100%). The other adhesion genes csgA, fimH and fimA were detected in 93%, 93%, and 73% of the isolates, respectively. The E. coli field isolates were screened blaTEM gene was detected in only four strains, which was categorized under strong biofilm producers. This study demonstrated the presence of biofilm forming E. coli in the raw chicken meat samples as contaminants, causing spoilage and potentially posing risk to consumer’s health and safety.