Pasteurella multocida D:4 producing Pasteurella multocida toxin (PMT) is a causal pathogen in atrophic rhinitis in pigs. To investigate the protective immunity and vaccination effect of recombinant PMT, the gene for PMT was isolated from the infective P. multocida D:4. The 2.3 kb XhoI/PstI fragment (PMT2.3) of PMT gene was expressed in E. coli BL21 (DE3) using the induced expression vector system. The recombinant protein of PMT2.3 having molecular weight of 84 kDa was purified by Ni-affinity column chromatography.

Environmental mastitis has increased particularly in well-managed or low somatic cell count herds that have successfully controlled contagious pathogens. Major pathogens of environmental mastitis are Escherichia coli (E. coli) and Streptococcus uberis. The present study was conducted to investigate the isolation and antibiotic susceptibility of 406 (21.8%) gram-negative bacteria isolated mastitis milk from 1,865 quaters of 241 Korean dairy farms from 2001 to 2004. Prevalence of major gram-negative bacteria isolated from mastitis milk were E. coli (22.7%) and Enterobacter spp. (16.3%) in coliforms and Pseudomoas spp. (10.3%) and Serratia spp. (7.9%) in non-coliforms.

Cutaneous asthenia or dematosparaxis is an uncommon, congenital and inherited connective tissue disease of dog and cat, resembling Ehlers-Danlos syndrome (EDS) in man. EDS is characterized by loose, hyperextensible and, fragile skin, it is attributed to mutations in connective tissue gene. These mutations cause defects in type Ⅰ or Ⅲ collagen synthesis and as a result lack of strength or elasticity to skin, joint, ligament and vessels. EDS-affected animals often experience subcutaneous hematomas that have long bleeding times.

A fourteen years old male oriental white stork from Korea Institute of Oriental White Stork Rehabilitation Research was presented to Veterinary Medical Center of Chungbuk National University with anorexia and lameness for 5 days. Bilateral intertarsal joint swellings were observed in physical examination. The radiographic findings indicated degenerative changes of joint cartilage and surrounding bones. In cytologic examination of synovial fluids, mononuclear leukocytic inflammation was identified. In blood films, a few fungal hyphae were observed and 2 fungal colonies were identified in blood culture on Sabroud dextrose agar.

A 3-year-old, intact female, Shih-tzu dog was presented with a 15-day history of vomiting, depression, and anorexia. On physical examination, systolic ejection murmurs with precordial thrill at the left heart base were detected. A diagnosis of congenital pulmonic stenosis (PS) was made mainly from the thoracic radiography, electrocardiography, and echocardiography. On complete blood counts and serum biochemistry profiles, the dog had already chronic renal failure (CRF). Thoracic radiography confirmed that main pulmonary artery was tremendously buldged and electrocardiography was suggestive of severe right ventricular hypertrophy.

Bone graft had been investigated previously to restore bone defects in orthopedics. The purpose of this study was to investigate the efficacy on new bone formation in bone autografts by treatment of implants. Cortical bone autografts were transplanted to midshaft of diaphyseal fibulae of 9 rabbits which were divided in 3 groups according to the treatment method of implants. Cortical bone implants for graft were treated with 3 different methods; freezing, freeze-drying, defat-freezing. Autografts were achieved by cross-transplantation method to bilateral fibulae of the presented rabbits after implant treatment procedures.

We investigated the safety, immunogenicity and protectivity of mix-crude outer membrane protein (cOMP) vaccine against salmonellosis in animals. The mix-cOMP vaccine was extracted from Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) and Salmonella enterica serovar Braenderup (SB) isolated from pigs. The mix-cOMP vaccine gave significantly higher antibody response than ST-bacterin and ST-cOMP vaccine in guinea pigs. The survival rates of mix-cOMP vaccinated groups showed significantly higher (100%) than those (0-20%) of unvaccinated control group, challenged with 3 species of Salmonella (ST, SE and SB) in mice.

Lactic acid bacteria (LAB) has been regarded as a useful microorganism and tried to manipulate plasmid DNA for increasing the usefulness. Although several methods have been developed is isolate plasmid DNA from Escherichia coli (E. coli), these methods were not sufficient to apply to LAB with exception of O'Sullivan's lysis method. So, we evaluated plasmid DNA extraction from LAB using general E. coli preparation methods and tried to improve the extraction yield and DNA purity by modifying O'Sullivan's alkaline lysis method.

A 7-year-old Jeju native boar was requested to the Veterinary Pathology Laboratory of Cheju National University with a clinical signs of anorexia, melena, lethargy and sudden death. At necropsy, four coalescing firm masses were occupied in the abdominal cavity between ventral surface of stomach and pancreas. Individual mass was a yellowish white in color and up to 6 cm in diameter. These masses were not encapsulated and bulged from the cut surface. Liver and spleen were enlarged and pale nodules were presented in these tissues.

This experiment has been investigated in order to examine larval migration inhibition activity of ivermectin, doramectin and ethanol against Anisakis simplex (A. simplex) in vitro. A. simplex larvae were obtained from the mackerel acquired from the fish market of Cheongju. They were divided into many groups and placed in culture dishes (40 larvae each) containing RPMI-1640, in the absence or presence of different concentrations of ivermectin, doramectin and ethanol. Ivermectin had a complete inhibition of larval migration at 72 h in all groups (10-300 ㎍/ml).