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النتائج 261 - 270 من 283
Isolation of enteropathogenic Escherichia coli, thermophilic Campylobacter and Salmonellae from scouring piglets
1988
Lee, J.H. | Cho, H.T. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Agriculture) | Kim, Y.H. | Kang, H.J. | Cha, I.H. (Kyongnam Animal Health Laboratory, Chinju (Korea R.))
This study was conducted to isolate etiological agents from the 103 scouring piglets in Kyongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical survey. The incidence of scouring piglet was most prevalent in 81.6% at the age of 2 to 4 weeks after birth, while the rate was less than 10% at the age of 5 to 6 weeks and under 1 week after birth. When comparing the isolation frequency of each etiological agent, enteropathogenic E. coli was most prevalent in 46.6%, thermophilic Campylobacter in 26.2% and Salmonellae was in 8.7% in that order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85(20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni belonged to type I (50.0%) and II (25.0%), and most strains of C. coli belonged to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C and 1 strain each was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, whereas about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C. jejuni and C. coli were sensitive to kanamycin and gentamicin
اظهر المزيد [+] اقل [-]Enterotoxin production and plasmid detection of citrate utilizing Escherichia coli isolated from cattle
1988
Chae, T.C. | Choi, W.P. (Kyongbuk National Univ., Taegu (Korea R.). Coll. of Veterinary Medicine)
This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli (Cit+ E. Coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 Cit + E. Coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains (11) were untypable. Thirty-nine(81.3%)out of 48 Cit + E. Coli produced colicin and 13(27.0%) produced hemolysin. Of 12 cit + E. coli bearing K99 antigen, 6(50.0%) produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 Cit+ E. Coli. Its molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid commonly existed in all strains
اظهر المزيد [+] اقل [-]Prevalence and morphological identification of Eimeria bukidnonensis and E. wyomingensis in cattle, Korea
1988
Wee, S.H. | Kang, Y.B. | Kim, S.H. (Rural Development Administration, Anyang (Korea R.). Veterinay Research Institute)
To determine the prevalence and the morphological identification of two Eimeria species in fecal examinations, a total of 1092 fecal specimens collected during the period of March to December in 1987 was tested by means of flotation technique. The prevalence of two species was revealed as 1.83% and 0.92% in E. bukidnonensis and E. wyomingensis, respectively. The oocysts of E. bukidnonensis measured in range of 41.3 micro m to 49.4 micro m (mean 45.5+-1.7) x 28.8 micro m to 35.3 micro m (mean 32.2 +-1.2), and E. wyomingensis 36.9 micro m to 44.5 micro m (mean 39.8+-1.7) x 26.5 micro m to 29.9 micro m (mean 28.2+-0.9). The oocysts of E. bukidnonensis appeared darker and more robust than those of E. wyomingensis. The striations in the oocyst wall of E. bukidnonensis were more peculiar than in E. wyomingensis. Among the morphological characteristics, the widths could be reliably taken as a differential point of view for two Eimeria species
اظهر المزيد [+] اقل [-]Effects of verapamil on norepinephrine-, phenylephrine- and clonidine-induced pressor response in rabbits and rats
1988
Shin, D.H. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Veterinary Medicine) | Choi, S.H. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Pharmacy)
To examine the selectivity of verapamil, used in the cardiovascular diseases, on alpha-1 and alpha-2 adrenoceptor-induced pressor responses, effects of verapamil on alpha-adrenoceptor agonist-induced pressor responses were investigated in urethane-anesthetized rabbits, spinal rabbits, rats and pithed rats. To evaluate the effects of verapamil on each pressor response induced by norepinephrine, phenylephrine and clonidine, these agonists were previously injected into an ear vein, and the same procedures were performed 1-2 min after treatment with intravenous verapamil. Intravenous verapamil produced dose-dependent depressor response in rabbits and rats. Pressor responses to intravenous norepinephrine (10 micro g/kg) and phenylphrine (30 micro g/kg) were inhibited by pretreatment with intravenous verapamil in rabbits and no difference was noted between the degree of both inhibitions of the pressor response by verapamil. Pressor responses to intravenous norepinephrine (3 micro g/kg), phenylephrine (20 micro/kg) and clonidine (300 micro g/kg) were inhibited by pretreatment with intravenous verapamil in spinal rabbits. No difference was noted between the inhibition of norepinephrine-induced pressor response and that of phenylephrine-induced pressor response by verapamil. The inhibition of clonidine-induced pressor response by verapamil was more prominent than that of norepinephrine- or phenylephrine-induced pressor response. Pressor responses to intravenous norepinephrine (3 micro g/kg) and phenylephrine (10 micro g/kg) were inhibited by pretreatment with intravenous verapamil in rats and no difference was noted between the degree of both inhibitions of the pressor response by verapamil
اظهر المزيد [+] اقل [-]Studies on vaginal cytology after induction of estrous in the bitch
1988
Kang, B.K. | Choi, H.S. | Son, C.H. | Oh, K.S. | Lee, C.B. (Chonnam National Univ., Kwangju (Korea R.). Coll. of Agriculture) | Na, J.S. (Chonanm National Univ., Kwangju (Korea R.). Coll. of Agriculture)
The present study was carried out to investigate the estrous behavior and vaginal smear after induction of estus with exogenous hormones in the premature, metestrous and anestrous bitches. In a total of 21 bitches(Mixed breed: 16, Jindo breed: 5), from 10 months to 5 years of age and weighing 8 to 15 kg the change of vaginal smear and the estrous behavior before and after induction of estrus was studied. In the treatment A(They were given the PGF2alpha, estrone, estradiol-17beta, PMSG and HCG) proestrus commenced in 10.16+-1.44(Mean+-SEM) days after treatment. The mean duration of proestrus and estrus was 7.50+-1.44 and 13.50+-3.44 days, respectively. In the treatment B(They were given the PMSG and HCG) proestrus commenced in 5.53+-0.59 days after treatment. The mean duration of proestrus and estrus was 6.60+-0.71 and 14.60+-1.14 days, respectively. All of the 6 bitches in the treatment A showed vulval swelling and vaginal discharge. 14 of the 15 bitches in the treatment B showed vulval swelling and vaginal discharge. However, all of the treatment A and B showed male acceptance. The main change of vaginal smear in proestrus and estrus after induction of estrus was an increase in the proportion of anuclear and superficial cells associated with a decrease in small intermediate and parabasal cells. By the estrous behavior and vaginal smear the estrus was induced in all the premature, metestrous and anestrous bitches
اظهر المزيد [+] اقل [-]Experimental infection with Campylobacter jejuni and Campylobacter coli in chicks
1988
Chung, B.G. | Kang, H.J. (Kyongsang National Univ., Chinju (Korea R.). Dept. of Veterinary Medicine)
The present study was carried out to investigate C. jejuni and C. coli in chicken. Also were examined the pathogenicity of the isolates in chicks by experimental inoculation. Thermophilic Campylobacter were isolated from 34 (45.9%) of the 74 specimens, and classified as 21.6 % C. jejuni, and 24.3 % C. coli. In the biotyping of 16 strains of C. jejuni isolates, 37.5 % of the strains were grouped as biotype I, 62.5 % as biotype II. In the case of 18 strains of C. coli isolates, 49.9 % of isolates were grouped as biotype I, 55.6 % as biotype II. In oral inoculation with 10** (4)cfu of Capmylobacter isolates into infant chicks (1 to 3 days-old), 17 days-old and 34 days-old chicks, 32.5 % of the chicks developed diarrhea on day 1, 52.5 % on day 3, 70.0 % on day 5, and 27.5% on day 7, and the peak incidence of diarrhea was reached on day 5. The organisms were found to be discharged in feces one day afterwards. C. jejuni and C. coli strains were detected from the feces in 87.5 % of the chicks on day 5.
اظهر المزيد [+] اقل [-]Studies on the internal decontamination of radioiodine
1988
Chung, I.Y. | Kim, T.H. | Chung, H.W. | Chin, S.Y. | Yun, T.K. (Korea Advanced Energy Research Institute, Taejon (Korea R.))
Appreciable radiation exposures certainly occur in the workers who handle radioiodine in biochemical research, nuclear medicine diagnostics with the development of nuclear industries. But in the case of nuclear accidents, the early medical treatment of radiation injury should be necessary but little was reported in Korea till now. Accordingly, to achieve basic data for protective roles and medical treatment of radiation injury, the present studies were carried out to evaluate the decontamination of radioiodine by the administration of the antithyroid drugs. The administration of sodium iodide and potassium iodide results in rapid excretion of radioiodine and reduction of the whole body retention than the saline-only group. Regarding thyroid protective effects, sodium iodide, potassium iodide results in rapid excretion of radioiodine and reduction of the whole body retention than the saline-only group. Regarding thyroid protective effects, sodium iodide, potassium iodide and saline were effected significant in order. In the control(saline) group, if administered with enough fluids, the whole body retention of radioiodine is reduced temporary shifts. But as far as radioprotective effects are concerned, saline was not more protective than the other groups. In conclusion, in case of nuclear accidents, if being administered sodium iodide and saline as quickly as possible, the radioprotective effects against the radiation hazard might be markedly increased in the internal contamination of radioiodine
اظهر المزيد [+] اقل [-]Studies on hematologic values and types of blood protein in [Korean] Jindo dogs, 1. Hematologic values of Jindo dogs
1988
Kim, W.K. | Han, B.K. (Chonnan National Univ., Kwangju (Korea R.). Coll. of Veterinary Medicine) | Kim, J.S. (Chonnam Animal Health Laboratory, Kwangju (Korea R.). Kangjin Branch)
The present study was conducted in order to get the normal hematologic values of Korean Jindo dogs. Blood samples were taken from 328 (male 92, female 236) healthy Jindo dogs in Jindo area. The mean values of red blood cell (RBC) count, hemoglobin (Hb) content and packed cell volume (PCV) in the age group of less than one year were 6.77(male 7.44, female 6.76)X10** (6)/ul, 13,14(male 13.53, female 12.99)g/100ml and 43.28(male 44.47, female 42.79)ml/100ml, respectively, whereas the RBC count, Hb content and PCV in the age group of one year and more were 7.42(male 7.44, female 7.42)X10** (6)/ul, 14.98(male 15.56, female 14.76)g/100ml and 47.18(male 48.43, female 46.71)ml/100ml, respectively. The values of RBC count, Hb content and PVC appeared to be higher in the male than in the female and it increased with aging in the age group of less than one year. The mean values of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin content (MCHC) in the age group of less than one year were 63.93(male 65.40, female 63.30)fl, 19.41(male 19.90, female 19.22) pg and 30.36(male 30.43, female 30.36)g/100ml respectively, whereas the MCV, MCH and MCHC in the age group of one year and more were 63.58(male 65.09, female 62.95)fl, 20.19(male 20.91, female 19.89) pg and 31.75(male 32.13, female31.60)g/100ml respectively. The values of MCV, MCH and MCHC appeared to be higher in the male than in the female. No differences were found in the MCV and MCH between age groups. The MCHC appeared to be higher in older age group. Mean platelet count in the age group of less than one year was 377,391(male 398,778, female 368,721)/ul, whereas mean platelet count in the age group of one year and more was 354,657(male 373,660, female 347,512)/ul. The platelet count appeared to be higher in the male than in the female and especially lower in the age group of three years and more (305,513/ul) than in the other age groups
اظهر المزيد [+] اقل [-]Morphological observations on the subependymal layer of swine brain
1988
Kwak, S.D. (Kyeongsang National Univ., Chinju (Korea R.). Coll. of Agriculture)
Normal swine brains at 1 to 70 days after birth were used to investigate the presence and morphology of the subependymal layer (SL) in the ventricle walls. The brain samples were taken from 27 pigs of 4 swine breeds. SLs were observed on the walls of the lateral ventricle (LV) but none were observed on the walls of the 3th and 4th ventricles. SLs of the LV walls were composed of mainly 3-to 10-cell layers in thickness. The thinnest region of SLs was composed of only 1-to 2-cell thick on the dorsal and ventral walls, and the thickest region was composed of 250-to 300-cell thick on extension region of the SLs into the angle between the corpus callosum and caudate nucleus. SLs were extensively thick in young age but gradually decreased in size and cell number with age after 20-day age. SL layers were composed of mainly oligodendrocytes, astrocytes and immature cells of them. Morphological differences of SL in different breeds of pigs were not observed.
اظهر المزيد [+] اقل [-]Studies on enzyme immunoassay for determining progesterone of bovine plasma and its clinical application: 1. Optimizing double antibody for progesterone in enzyme immunoassay
1988
Kang, C.B. | Shin, J.U. | Choe, S.Y. (Kyongsang National Univ., Chinju (Korea R.). Coll. of Agruculture)
This experiment was carried out to determine the progesterone concentration of bovine plasma by liquid phase double antibody enzyme immunoassay. The optimum conditions of assay-system, double (first and second) antibody and carrier (normal rabbit serum) were investigated. The optimum dilution rate of first antibody, second antibody and normal rabbit serum was 10X10** (3) to 15X10** (3), 20 and 1X10** (3) times, respectively
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