Condensation of 6-methoxy benzothiazol-2-amine with 2-hydroxy- naphthaldehyde gave newSchiff-base derivative in good yield. The metal complex of Co(II) have been synthesized withSchiff base of benzothiazol derivative. Spectroscopic study such as, two dimensional NMR ofnew compound have been obtained by using nuclear magnatic resonance 600 MHz.Computational study of all compounds was calculated using Gaussian 09 program package. Thesynthesized compounds were screened for their antibacterial activity against Staphylococcusaureus, Escherichia coli, Bacillus subtilis, Klebsiella pneumonia. Additionally, the compoundswere tested for antifungal activity against Candida albicans, Candida tropica, Aspergillus multiand Aspergillus niger. Cobalt complex compound exhibited more activity as antifungal thanbenzothiazole derivative.

The current study was conducted from October 2013 until the beginning ofSeptember 2014 to estimate the Omega -3fatty acid for three species of marine fish(Acanthopagrusarabicus , Chelonsubviridis and Cynoglossusarels). The study wasconducted during the four seasons winter of 2013, spring of 2014 , summer of 2014and fall of 2014. The oil was extracted by organic solvent extraction, the fatty acidwas estimated using Gas Chromatography connected with mass spectrometry(calculated on the basis of the total amount of the fatty acids), omega–3 percentage ofboth types DHA, EPA has varied between the fish as it reached 56.5% , 55.9% ,67,13% , 49.22% during winter, spring, summer and autumn respectively. Also it wasfound ,by the results, that the percentage of omega -3 type EPA was high in the winteras the percentage was 30.99%, followed by the summer as it was 30.42% and thelowest rate was in the autumn as it was 20.33%, while the percentage of the secondtype of omega -3 DHA was close during the four seasons, as the summer achieved thehighest percentage which was 36.71% and the lowest percentage was in the winter asit reached 25.06%.

Eight Samples of tumor masses were collected from cases of (3)squamous cellcarcinoma in vulva (SCC) and(5) ruminalfibropapillama in cows fromBasraprovincesouth of Iraq during the period February 2013 through January 2014 . The cases of SCCcases were characterized by smooth, dry, white poorly demarcated dermal bundles ofhomogenous tissue with necrotic or\and hemorrhagic foci separated by fibrous tissue intheearlyand late stage named as acanthosis . The size offibropapilloma cases werevariable in size and characterized byabundant proliferating fibrous tissue massgrowth inthe rumen covered by stratified squamous epithelium of varying thickness exhibitingmarked hyperkeratosis of the stratumcorneum of rumen.

The objective of this study was to develop a murine retinal/choroidal/scleral explant culture system to facilitate the intravitreous delivery of vectors. Posterior segment explants from adult mice of 2 different age groups (4 wk and 15 wk) were cultured in serum-free medium for variable time periods. Tissue viability was assessed by gross morphology, cell survival quantification, activated caspase-3 expression, and immunohistochemistry. To model ocular gene therapy, explants were exposed to varying transducing units of a lentiviral vector expressing the gene for green fluorescent protein for 48 h. Explant retinal cells remained viable for approximately 1 wk, although the ganglion cell layer developed apoptosis between 4 and 7 d. Following vector infusion into the posterior segment cups, viral transduction was noted in multiple retinal layers in both age groups. An age of donor mouse influence was noted and older mice did not transduce as well as younger mice. This explant offers an easily managed posterior segment ocular culture with minimum disturbance of the tissue, and may be useful for investigating methods of enhancing retinal gene therapy under controlled conditions.

Brucella melitensis is the Brucella species most frequently associated with brucellosis in humans. It is also the causative agent of the disease in goats and other ruminants. Although significant aspects of the pathogenesis of infection by this intracellular pathogen have been clarified, several events during invasion of host cells remain to be elucidated. In this study, infections of human macrophages from the THP-1 monocyte cell line were conducted with B. melitensis Bm133 wild-type strain and a strain of Salmonella serovar Enteritidis as a control. A multiplicity of infection of 100 was used in trials focused on defining the relative expression of syntaxin 4 (STX4), a soluble N-ethylmaleimide-sensitive factor attachment protein receptor, in the early events of phagocytosis (at 15, 30, 45, and 60 min). Immunoblot assays were also done to visualize expression of the protein in cells infected with either bacterial strain. The expression of STX4 was not significantly different in cells infected with B. melitensis strain Bm133 compared to that observed in cells infected with S. Enteritidis. When the expression of STX4 mRNA was inhibited with short or small interfering, or silencing, RNA in the THP-1 cells, the survival of B. melitensis was significantly reduced at time 0, when gentamicin treatment of cultures was begun (after 1 h of phagocytosis), and also at 2 h and 12 h after infection.