Recent studies have identified wing cells, a substructure of the wing, as carrying taxonomic signals across various mosquito genera. However, the presence of a taxonomic signal in the wing cells of the genus Lutzia (Diptera: Culicidae) has not yet been confirmed. Objective: This study aimed to evaluate the efficacy of the outline-based geometric morphometric (GM) approach in distinguishing Lutzia species found in Thailand, namely Lutzia chiangmaiensis, Lutzia fuscana, Lutzia halifaxii, and Lutzia vorax. Materials and Methods: The outline-based geometric morphometrics was employed to analyze four wing elements: the wing contour, the second submarginal cell, the first posterior cell, and the third posterior cell. Results: In the size analysis, Lt. vorax consistently exhibited significantly larger wing elements compared to the other species (p < 0.05). The factor maps based on discriminant analysis for the wing elements among the species indicated that most groups overlapped in morphospace. However, for the third posterior cell, the Lt. vorax group presented a more distinct shape. While shape analysis detected significant differences between almost all species pairs (p < 0.05), there was an exception between Lt. halifaxii and Lt. chiangmaiensis in the first posterior cell (p > 0.05). Additionally, shape analysis further indicated that the third posterior cell achieved the highest percentage of correct classifications, with an adjusted total assignment accuracy of 71%. Conclusion: This finding reveals a significant taxonomic signal in the third posterior cell, suggesting that the outline-based GM approach can effectively complement the landmark-based GM approach in distinguishing Lutzia species. J. Adv. Vet. Anim. Res., 12(2): 516–528, June 2025 http://doi.org/10.5455/javar.2025.l916

Objective: This study aimed to evaluate the effects of plant extracts from desert species on egg-hatching inhibition (EHI) and larval mortality of gastrointestinal nematodes (GINs) in cattle under in vitro conditions. Materials and Methods: Hydro-alcoholic extracts of tasajillo [Cylindropuntia leptocaulis (DC) F.M. Kunth], coyonoxtle [Cylindropuntia imbricata (Haw) F.M. Kunth], mariola (Parthenium incanum Kunth), and mesquite [Neltuma juliflora (Sw.) Raf.] were tested. GIN eggs and infective larvae were exposed to the plant extracts in decreasing doses, and the median lethal dose50 (LD50) and maximum lethal dose99 (LD99) were calculated using the SAS PROBIT procedure. Results: The C. imbricata extract demonstrated the highest EHI at a small LD50 (2.31 mg/ml) and achieved 100% larval mortality at a 5.8 mg/ml concentration. The P. incanum extract showed the highest larval mortality at the LD99 (6.50 mg/ml), although N. juliflora had the lowest LD50. However, the N. juliflora pod extract was the least effective overall. These findings indicate that C. imbricata was the most effective extract for inhibiting egg hatching, while P. incanum was the most effective for promoting larval mortality under in vitro conditions. Conclusion: The small doses used against eggs and larvae of nematode parasites suggest that wild desert plants could provide a viable and ecological alternative for the in vitro control of GINs in cattle. J. Adv. Vet. Anim. Res., 12(2): 529–538, June 2025 http://doi.org/10.5455/javar.2025.l917

Objective: This research aimed to isolate and identify potential white-rot fungi from various locations in West Sumatra, Indonesia, that could be used for biodelignification of animal feed ingredients. Materials and Methods: Wood samples with fungal infections were directly observed, and the visual method was employed to collect samples. The purified white-rot fungi isolates underwent the Bavendamm test to evaluate their biodelignification capabilities. Results: Eight of the 15 purified white-rot fungi isolates demonstrated positive results in the Bavendamm test, indicating their potential for biodelignification. Further analysis revealed the presence of three indigenous white-rot fungi species in West Sumatra: Exidia sp., Trametes sp., and Phanerochaete sp. These findings highlight the richness of white-rot fungi biodiversity in the region and underscore their suitability for lignin degradation in animal feed ingredients. Additionally, the successful isolation and identification of these fungi represent a crucial step toward sustainable biotechnological applications in livestock farming. Conclusion: The identified white-rot fungi have shown promising capabilities for lignin degradation in animal feed ingredients. However, further research is essential to ascertain the optimal enzyme ratio for lignin degradation and to enhance the identification techniques for a broader range of white-rot fungi species. This study provides a foundational step toward improving animal feed quality in the region, especially for ruminants. J. Adv. Vet. Anim. Res., 12(2): 558–564, June 2025 http://doi.org/10.5455/javar.2025.l920

Objectives: Kopi luwak (civet coffee) is produced through a fermentation process in the digestive system of civets. This study aims to investigate the diversity of microorganisms in Indonesian civet excreta fed with robusta coffee cherries. Materials and Methods: Six excreta samples were collected from male and female Indonesian civets of three species: binturong (Arctictis binturong), Asian palm civet (Paradoxurus hermaphroditus), and masked palm civet (Paguma larvata). Microbial diversity was analyzed using next-generation sequencing. Results: Based on alpha and beta diversity analysis, the microbial community in civet excreta differs. Microbes found in the excreta of male P. hermaphroditus (PH-M) were the most diverse. Microbes were identified in the bacterial and yeast domains. The relative abundance of bacteria was higher than yeast. The occurrence of non-pathogenic bacteria (50.76%–90.51%) was higher than pathogens (9.49%–41.24%). The dominant bacteria in the excreta of all civets were Escherichia coli (15.98%–54.68%). Although not dominant microorganisms, lactic acid bacteria (LAB) and yeast are present in civet excreta. The LAB present in the range from 0.16% to 32.14%, with the most abundant LAB being Streptococcus pasteurianus, Weissella confusa, and W. cibaria. Meanwhile, the identified yeasts were Hanseniaspora opuntiae and H. uvarum. Pathogenic bacteria, both spore-forming and non-spore-forming, were also present in civet excreta. The virulence factors and antimicrobial resistance gene cluster were detected. Conclusion: The microbial diversity of Indonesian civet excreta is influenced by species and sex. PH-M contains the most diverse microbes. The presence of foodborne pathogens in civet excreta may be carried over into the beans, and hence, further processing of the beans should assure the safety of the beans. J. Adv. Vet. Anim. Res., 12(2): 565–581, June 2025 http://doi.org/10.5455/javar.2025.l921

Objective: The purpose of the research was to assess possible diagnostic parameters related to subclinical mastitis in goats. Materials and Methods: Individual samples of milk from goats were separated into three groups based on the chloride concentration: group I < 70 mmol/l; II group 70–85 mmol/l; III group > 85 mmol/l. The composition of milk was studied, and smears were also made. Results: When the chloride content in goat milk exceeds 85 mmol/l, the somatic cell count increases by 3.2–5.7 times in relation to milk with a chloride content below 70 mmol/l, depending on the research method used. In the second group, the indicators exceed those of the first group by 2.1–3.8 times. Compared to the second and third groups, the indicator of electrical conductivity in goats of the first group was lower by 13.1% and 31.3%, respectively. Milk from healthy goats, characterized by a chloride content of less than 70 mmol/l, shows a chlorosugar number averaging 5 (ranging from 4.1 to 5.9). In cases where chloride levels in milk exceed 85 mmol/l, the average indicator of the chlorosugar number is 7.2 (from 6.5 to 7.9). If the content of chlorides is > 85 mmol/l, a positive result of the settling test with goat’s milk is noted. Conclusion: A set of indicators such as chloride content > 85 mmol/l, the number of somatic cells >2 million/ml, chlorosugar number ≥ 7, along with confirmed results in the settling and mastidine tests—can be used as a diagnostic criterion for subclinical mastitis in goats. J. Adv. Vet. Anim. Res., 12(2): 582–588, June 2025 http://doi.org/10.5455/javar.2025.l922

Objective: The aim of this study was to examine the solvent conditions suitable for preparing samples of total mixed ration (TMR) silage and ruminal fluid, with or without chitosan inclusion, to achieve more sensitive, specific, and repeatable analyses. Material and Methods: The metabolome contained in the sample was determined using a ultrahigh-performance liquid chromatography-high-resolution mass spectrometry quadrupole using samples in the form of TMR silage (silage with 0.5% inclusion of chitosan, silage without chitosan addition) and rumen fluid (rumen fluid containing 0.5% chitosan, ruminal fluid without chitosan) with and without the addition of chitosan, as well as three different solvent ratios: 50% water MS grade: 50% methanol (MeOH) MS grade (1), 20% water MS grade: 80% MeOH MS grade (2), and 0% water MS grade: 100% MeOH MS grade (3). Results: The TMR silage samples contained 311 metabolites, amino acid compounds predominating, followed by fatty acids and amines. Fatty acids, organic phosphate, and amines dominated the 39 metabolites found in rumen fluid samples. In the TMR silages, 100% MeOH seemed to be able to distinguish samples more effectively than other solvents; moreover, cinnamic acid, D-(+)-proline, and L(+)-ornithine were the three highest variable importance for projection values among prospective metabolite markers for silage samples. Whereas the use of a 50% MeOH to 50% water solvent ratio achieved the best discrimination results in rumen fluid samples, the three highest variable importance for projection values among prospective metabolite markers for ruminal fluid samples were triethyl phosphate, dibenzylamine, and phosphoric acid. Conclusion: 100% MeOH is able to distinguish TMR silage, while 50% MeOH to 50% water is best for ruminal fluid samples. J. Adv. Vet. Anim. Res., 12(2): 589–600, June 2025 http://doi.org/10.5455/javar.2025.l923

Objective: To describe the clinical presentation, diagnostic imaging findings, and management of a rare case of bilateral temporomandibular joint (TMJ) ankylosis combined with dysplasia in a cat. Materials and Methods: A 4-month-old Bombay male cat, normally immunized and dewormed. Radiographic studies: Details of imaging techniques used to diagnose TMJ ankylosis and dysplasia: computed tomography (CT) scans were used to visualize the joint structures in greater detail using CT. Results: Out of the 21 blood biochemical abnormalities found in the laboratory test, creatinine, albumin, albumin/globulin, and total cholesterol all showed a significant decrease. The levels of phosphorus and α-amylase were marginally elevated. A three-dimensional CT scan revealed a malformed fusion of the right TMJ zygomatic arch and bilateral mandibular coronal process. Both TMJ dysplasia and ankylosis were discovered by the biochemical and physical 3-dimensional CT scans. Conclusion: The findings underscore the importance of a thorough clinical examination and imaging studies to assess the extent of the ankylosis and any associated dysplastic changes. This case emphasizes the need for increased awareness of TMJ disorders in felines and encourages further research into effective treatment protocols. J. Adv. Vet. Anim. Res., 12(2): 321–326, June 2025 http://doi.org/10.5455/javar.2025.l899 

Objective: This study aimed to determine the seroprevalence of anisakiasis and its associated risk factors in the Sangihe Islands Regency, Indonesia, where people have a unique habit of consuming raw seafood “Kinilo.” Materials and Methods: This cross-sectional study involved 112 respondents who met the inclusion criteria using the Indirect ELISA method with the Anisakidae immunoglobulin G (IgG) kit. Results: The results showed that 59/112 respondents (52.67%) were seropositive for Anisakidae IgG, with significant risk factors such as the consumption of Katsuwonus pelamis and measures related to eating habits. Multivariate analysis revealed that consumption of raw K. pelamis was associated with a 45.748 times higher risk of anisakiasis. Conclusion: These findings highlight the need to raise awareness and implement interventions to prevent infection, including fish processing and storage education. This study emphasizes the need for a holistic health approach to reduce the risk factors for anisakiasis in at-risk communities. J. Adv. Vet. Anim. Res., 12(1): 8–18, March 2025 http://doi.org/10.5455/javar.2025.l867

Objective: This study aimed to identify the impact of variations in solvent polarity on the solubility of secondary metabolite compounds, which were correlated as antioxidant and antibacterial agents for Escherichia coli (ATCC 11725), Salmonella sp. (ATCC 22504), Staphylococcus aureus (ATCC 11526), and Bacillus subtilis (ATCC 11626). Materials and Methods: A total of 500 gm of Temulawak flour (Curcuma xanthorrhiza) was macerated gradually using the step gradient polarity (SGP) technique, where the solvents (methanol, ethyl acetate, and n-hexane) used had different levels of polarity. The extracting process of secondary metabolite components of Temulawak flour began with the use of non-polar solvents, semipolar solvents, and polar solvents, respectively. Each soaking process was completed for 5 × 24 h. Results: From the Temulawak extract with different levels of solvent polarity, 76.048 gm were obtained for the methanol fraction, 106.242 gm for the ethyl acetate fraction, and 154.575 gm for the n-hexane fraction. The results of the antioxidant activity test showed that the ethyl acetate fraction had the highest inhibitory value for antioxidant activity of 50% (IC50) 22.59 mg/l. The results of this research’s β-carotene content were 0.0865 gm. Ethyl acetate fraction was known to have good activity from the antibacterial activity test. The inhibition zone of E. coli and B. subtilis bacteria was 11.5 ± 0.71 with a minimum concentration of 3.13 mg/ml. The inhibition zone of S. aureus was 10.5 ± 0.17 with a minimum inhibitory concentration (MIC) of 6.25 mg/ml, while the inhibition zone for Salmonella sp. was 8.125 ± 0.35 with a MIC of 37.50 mg/ml. Conclusion: All fractions have moderate antibacterial activity, yet the ability of the ethyl acetate fraction of Temulawak extract was higher than the methanol and n-hexane fractions. J. Adv. Vet. Anim. Res., 12(1): 192–204, March 2025 http://doi.org/10.5455/javar.2025.l886

Objective: The objective of this study was to evaluate the integrity of the plasma membrane for human and other domestic animal spermatozoa by the hypo-osmotic swelling test (HOST). Materials and Methods: This study was conducted on spermatozoa of three species, including pigs, rabbits, and goats. Three experiments were conducted on three animal species (pigs, rabbits, and goats) to investigate the factors affecting sperm membranes: osmotic concentration (0, 50, 100, and 150 mOsmol) and treatment time (0, 15, 30, 45, 60, and 90 min) at 37°C. Results: The results showed that treatment time and osmotic concentration influenced the integrity of the sperm membrane in the hypo-osmotic solution. In general, the experiments showed a high rate of sperm reacting to HOST at 50 and 100 mOsmol for 30–45 min. According to this study, the hypo-osmotic solution resulted in a high rate of sperm with swollen tails and a high rate of viable sperm, indicating a correlation between these factors. Therefore, it is necessary to combine both conditions to assess sperm quality. Specifically, the positive rates for the HOST reaction in pig, rabbit, and goat sperm are 46.74%, 58.28%, and 61.95%, respectively. Conclusion: To sum up, the hypo-osmotic solution with a concentration of 100 mOsmol and an incubation time of 45 min is considered the optimal and most feasible condition that can be used for all three species: pigs, rabbits, and goats. J. Adv. Vet. Anim. Res., 12(2): 477–486, June 2025 http://doi.org/10.5455/javar.2025.l913