Research of the influence of biologically active preparations of natural origin on zoological indices and quality of animal products was studied by the example of their application in broiler chicken diets in the conditions of the Republic of Belarus. On the basis of realized research it was established, that application of biologically active substances immunoamplifier Alveozan, probiotic Dialakt, probiotic Bifidoflorin liquid, prebiotic Biofon and prebiotic Biofon AIL (produced in the Republic of Belarus) did not render negative effect on quality of animal products (all studied meat samples had high quality). The received results testify to necessity of complex application of the above-stated preparations. Application of biologically active preparations made it possible to increase livability of growing birds on 100%, average live weight increase from 1,9 up to 5,1%, daily average weight gain - from 1,9 up to 5,1%, improvement of feed conversion ratio - from 18,3 up to 21%. All studied biologically active preparations made it possible to restore the physiological environment in poultry intestines, normal flora, to suppress the growth of pathogenic microorganisms. The above mentioned preparations could be applied both with preventive, and with the medical purposes

Traditional raw materials in Belarus for mixed fodders production are wheat, barley, oats, rye, peas, lupine, sunflower and rape cakes, bran. However, productive action of these forages is limited because of presence of antinutritious factors and the raised cellulose content. In the Republic of Belarus there was organized production of national enzyme fodder additive Fekord 2004. Peculiar feature of the presented enzyme complex was addition of into its structure of enzyme glucoamylase, promoting hydrolytic starch splitting, and accordingly, the increasing of absorbency of difficult carbohydrates of forages. It was especially important for stores at early stages. Thereupon it was expedient to study the efficiency of application specified multienzyme composition in diets of growing and fattened stores. It was established, that enrichment of mixed fodder with the analysed fodder fermental additive at the rate of 0,13 and 0,15 kg per ton of mixed fodder made it possible to increase the growth rate of pigs and lower charges for forages per unit of body weight gain. Higher economic return on the fodder enzymic additive an additional gain of body weight appeared at its introduction in quantity of 0,15 kg per ton of mixed fodder

In animal feeding technology much attention is given to application of biologically active substances for metabolism stimulation. One of such biologically active substances is carnitine, an anabolic preparation of nonhormonal nature, cofactor in system of oxidation of fat acids. It participates in processes of acetylation at oxidation of fat acids together with acyl-CoA and other enzymes, promotes carrying over of cytoplasmatic Coa-derivatives of fat acids inside mitochondrions. It increases absorption speed from a digestive path of nutrients of forage, raises use of fat acids for the power purposes. Under its influence synthesis of triglycerides decreases and synthesis of phospho-lipids increases. Carnitine also protects cells against toxic accumulation of components of acyl-CoA of endogenic or exogenous origins, such as acylic groups of carnitine ethers which then can pass into liver for dissimilation or in kidneys for allocation in urine. Carnitine synthesis satisfies body requirements, but in the conditions of a hightened stress, high productivity, as well as in the conditions of lowered carnitine content in forage or in the conditions of the increased content of fats it should be applied as additive to diet. As a result of the realized study it was established, that the optimal quantity of carnitine application in diets for stores husbandry and fattening is dose of 50 g/t of mixed fodders. Application of carnitine in an optimum dose renders the positive impact on animal organism, promotes the increasing average daily weight gain on 5,1% (Р less than 0,001). Application of carnitine in diets of swine stores increases a slaughter yield on 2,1%, meat content per carcass - on 2,3%, a loin eye area - on 8,8%. Carnitine application in a dose 50 g/t of mixed fodders makes it possible to receive the additional profit at a rate of 4,57 USD per 1 head

Application of protein, vitamin and mineral supplement in diets of 1-3 months old calves in rate of 5% per weight instead of a part of sunflower cake, containing rape, peas, lupin and vitamid on a basis of halites, phosphite, phosphate, sapropel and a mineral-vitamin preparation in structure of grain forage against winter rations on the basis of rich in herbs hay (5%), milk (68%), mixed fodder (20%), whole grain (7%), and also summer rations with grass and legume mixture (7%), hay (1%), milk (65%), mixed fodder (20%), integral grain (7%) makes it possible to receive daily average weight gain of calves at level 833-867 grams at expenses of forages for 1 centner of weight gain on 3,3-3,4 сentner of forage units. Introduction into rations of the protein, vitamin and mineral supplement on the basis of local protein and mineral raw materials in number of 10% per weight in structure of mixed fodder for calves at the age of 3-6 months old against the background of winter diets with haylage (28%), mixed fodder (64%), molasses (8%), as well as summer diets with grass and legume mixture (30%), mixed fodders (66%), molasses (4%) makes it possible to receive daily average weight gains of calves on 944-967grams at expenses of forages of 4,0-4,1 сentner of forage units. Feeding of 1-6 months old calves with mixed fodders with inclusion of 5-10% of protein, vitamin and mineral supplement per weight with grain high-protein crops against the background of winter and summer diets with a ratio of the deconjugate protein to the non deconjugate one - 65:35 (in control group - 71:29) instead of sunflower cake makes it possible to receive the daily average weight gain at the level of 900-927 grams at expenses of forages 3,7-3,8 сentner per forage unit. Cost of presented mixed fodders decreases on 14%, and the cost price per 1 centner gain - on 7-8%

Activity of amylolytic, proteolytic, and lipolytic enzymes in the content and mucosa of intestinal tract, alkaline phosphatase in mucosa of intestinal tract, as well as the determination of calcium and phosphorous concentration in gastro-intestinal tract of hens were analyzed in the conditions of the Republic of Belarus. Results of analysis of the obtained experiment results of laying hens of parental stock by influence of various vitamin-mineral additives were presented. The highest activity of amylolytic, proteolytic, and lipolytic enzymes in 170-330 days old laying hens of Belarus-9 cross was marked in small intestine. There was stated the decreasing of enzymatic activity in proportion to removal from a stomach. At the same time, activity of alkaline phosphatase decreasds. Activity of alkaline phosphatase, proteolytic and starch-reducing enzymes in a mucous membrane of a duodenal gut was a bit higher than in mucosae of jejunal. Lipolytic enzymes showed high degree activity in mucosae of jejunal. Enzymic activity in large bowel was low. Addition of iodine-containing preparation Kajod into hen layer diets increased the activity of alkaline phosphatase, of amylolytic, proteolytic, and lipolytic enzymes in intestines. Enzymic activity of mucous intestines at hens of breed Plimutrok in process of removal from stomach decreased. In course of the period from 240 to 330-days old age the activity of studied enzymes in hen intestines decreased. Replacement of mineral additive Cockleshell by Pikumin did not render negative influences on changes in digestive enzyme activity. In 170-days old laying hens of Belarus-9 crossbreed the calcium content increased from the stomach to the small intestine, and then decreased in thick department. In the 200-days old hens the calcium concentration in a stomach increased in both groups and gradually decreased in the department of small intestine, but in rectum its quantity increased in both groups. In the period of intensive egg-laying (280 days) the general calcium content in thin department of intestines was less than in the stomach, later it increased in cecum and decreased in rectum. By the end of the experiment (330 days) the concentration of calcium in control group decreased from the stomach to the beginning of thick department of intestines, but in rectum its quantity increased. In experimental group the calcium content increased from a stomach to cecums and then decreased in rectum. In distribution of inorganic phosphorus in gastrointestinal tract departments at hens of various age groups the tendency to increase in its quantity in small intestine and the beginning of thick department of intestines, and then to reduction in rectum was observed. And at hens receiving premix Ajdeko, the phosphorus content, since 200-day age, was higher, than at hens receiving the basic ration

Determination of the efficiency of young fat stock feeding with wet rolled maize grain which was preserved by means of different preparations was realized in the conditions of the Republic of Belarus. The following preserving agents were applied: HV-2 (by- product of amino-formaldehyde resin); AIV 3 Plus (mixture of 62% formic acid and 24% ammonium formate; KEMIRA, Finland); carbamide (30 kg/t) with 40 g/t of complex mineral supplement. Feeding of young fat stock with rolled maize grains preserved by NV-2 promoted the increasing of nutrient digestibility on 1,5-5,0%, nitrogen use - on 2% in comparison with control. Application of rolled maize grain preserved with AIV-3Plus and carbamide with the complex mineral supplement made it possible to increase the digestibility of nutrients on 1,0-4,8% and nitrogen use - on 1,6-1,7%. Introduction of rolled maize grain preserved with NV-2 into rations of bull calves increased their productivity on 4,7% (P less than 0,05) with the simultaneous lowering of forage expenses on 1,9%. Application rolled maize grains preserved with AIV-3Plus made it possible to increase daily average weight gain on 3,8% and lower the forages expenses on 2,6%. Use preserved with NV-2 and AIV-3Plus rolled maize grains in rations of young fat stock lowered the production cost price on 11,9% and 10,8% that proved the efficiency of the studied fodder

Analysis of the efficiency of application of fermented milk supplementary feed DKM (Belarus) produced on the basis of bacteria strain Lactobacilus acidophilus 55/06 in calve diets was realized in the conditions of the Republic of Belarus. Influence of feeding with acidified with the trial milk additive on forage palatability, biochemical blood structure and calve productivity was studied; economic efficiency of DKM use for processing of whole milk and its use in rations of calves was defined. Experimental batches of DKM fermented milk supplementary feed were produced at Grodno Medical Preparations Plant (Belarus). It was issued in the sterile corked polyethylene 5, 10 and 20 liter bottles or canisters. The fermented milk supplementary feed DKM could be stored at the temperature from 0 deg С up to 15 deg С. Shelf-life – 3 months from the moment of production. The additive represents the homogeneous mass of viscous consistence of cream color of various shades with a sour-milk smell. The carried out economic calculations showed, that as a result of growing capacity increase it animals of a trial group and reception of an additional total live weight gain there was stated the 6,5% decrease of expenses for fodder unit production in the experimental group. However, due to a high cost of acidified milk the cost of fodder unit in trial group was on 6,8% higher. As a result, the cost price of 1 kg weight gain in trial group (despite high additional weight gains) was stated at the level of control group. Application of fermented milk supplementary feed DKM in calve diets made it possible to lower calf disease incidence and to increase calf productivity on 11,8% without increasing the cost price of production

Studying of interaction of phytolectins with carbohydrate epitopes of cattle erythrocytes and alpha 1-4 D-glukan was realize in the conditions of the Republic of Belarus was realized by the example of seeds of soy (Glycine max) of Viliya variety, common bean (Phaseolus vulgaris) of Olga variety, spring barley (Hordeum vulgare) of VM-MGF variety, and wheat (Triticum aestivum) of Akogomugi varity. Lectins studies of pathological conditions (infringement of a cellular metabolism, transformation and cell destruction, etc.) were closely connected with research of structure and functions of cellular membranes that was important for carrying out of various biotechnological works. As a result of the realized research there was given the comparative characteristic of hemagglutinin activity of phytolectins of some bean and grain crops in relation to erythrocytes of cattle and precipitating with and 1-4 D-glukan. Intensive precipitation of alpha 1-4 D-glukan was observed in the conditions of its interaction with lectins of soya and wheat and less expressed in the conditions of interaction with lectins of bean and barley. It was established, that glucose monomers and its derivatives proved to be the basic structural element of glycocalyx of erythrocytes which detected the phytolectins of soya, beans, wheat and barley

Improvement of the standards of energy and protein diets of young stock cattle of 1-6 month old and determination of its influence on animal productivity was realized in the conditions of the Republic of Belarus. The studied made it possible to determine that at the age of 1 month the calf diet should contain 1,02 kg of dry matter and 21,2 MJ of metabolic energy; at the age of 2 months - 1,26 kg and 23,5 MJ; at the age of 3 months - 2,2 kg and 25,6 MJ; at the age of 4 months - 2,9 kg and 31 MJ; at the age of 5 months - 3,28 kg and 34,2 MJ; at the age of 6 months - 3,76 kg and 37,6 MJ, respectively. Metabolic energy concentration per 1 kg of dry matter should not be lower than 20,7 MJ at the first month, 19 MJ - at the second month, after the third month it should lower up to 10 MJ. In course of 6 months the presented diet scheme made it possible get 832 g of average weight gain in the conditions of forage expenses of 3,52 forage units per 1 kg

In the Republic of Belarus the grain part of mixed fodders is basically formed by the following cereal cultures: wheat (Triticum aestivum), barley (Hordeum vulgare), oat (Avena saliva) and triticale. Rye is applied in the limited quantity in pig feeding despite all its advantages: large areas of cultivation, stable yielding capacity, low requirements to growing conditions, possibility to obtain ecologically clean production, low prime cost of grain. Rye in swine diets forms viscous solutions which break digestion process, worsen their fixation and accordingly reduce animal weight gain. Also, it was determined that rye grain had high quantity of pentosans (non-starch polysaccharides) which suppress the overall digestibility and accessibility of proteins. One of the methods of decreasing the negative influence of anti-nutritional properties of rye on pig organism and improving its digestibility is the application of fodder enzyme additives. In course of the realized investigation there was analyzed the influence of foreign fodder additives on rye-containing (Sk-21 – 10% and SK-26 – 20%) diets in swine rations. The following enzyme additives were analyzed: Rovabio TM Excel (France); Finase TM (Germany); Avizyme and Porzyme 8100 TM (United Kingdom). Application of enzyme additives Avizyme, Rovabio and Finase in mixed fodders containing rye (SK-21 - 10% of rye) for pigs at the age of 50-107 days made it possible to increase live weight on 16,8-21,7% and lower expenses for forages (fodder units) per 1kg of weight gain on - 12,3-18,2%. Intensity of live weight gain from 50-day up to 190-days age was the following: in a control group -80,8 kg, in the first experimental group fed with Rovabio - 88,6 kg, in the second experimental group fed with Avizyme - 89,7 kg, in the third experimental group fed with Finase - 84,6 kg. Research results showed that enzymes Avizyme and Rovabio rendered the highest influence on mixed fodders containing rye (SK-21 - 10% of rye and SK-26 - 20% of rye)