This study performed in twenty four male Sprague Dawley rats for (90) days which divided randomly into four equal groups. Group (1): received normal saline daily. Group (2): received orally codeine-paracetamol(40/2500 mg / kg b.w) daily. Group (3): received orally codeine-paracetamol (80/5000 mg / kg b.w)daily. Group (4): received orally codeine- paracetamol (160 /10000 mg / kg b.w) daily. After end of day (90) of study all animals were sacrificed to do the histopathological and biochemical examinations. The statistical analysis results revealed the body weight effects of codeine-paracetamol toxicity a significant (P≤0.05) decreases of treated group after (90) days of treatment. The histopathological investigation of liver, kidney and brain of treated groups showed centrolobular necrosis, dilation of sinusoids, vaculation of hepatocytes and septal fibrosis of liver while kidney showed vaculation of mesengial cells of glomeruli, necrosis of proximal convoluted tubules and dilation of renal cortical tubules, also brain of treated group showed vaculation of neurons, these changes are appeared mild in group (2), moderated in group (3) and sever in group (4). The statistical analysis results of biochemical investigations of liver and kidney function tests showed a significant (P≤0.05) increases of levels of serum AST, ALT, ALP, bilirubin and creatinine respectively in all treated groups which these enzymes increased mildly in group (2), moderately in group (3) and severely in group (4).

In recent years, pyrazole and pyrimidine derivatives attracted organic chemists due to their widespread potential biological and chemotherapeutic activities. In this study, pyrimidine derivative namely 6-Chloro-2,4-diamino pyrimidine was screened for antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus cerius, Streptococcus spp, Klebsella- spp and salmonella spp and fungicidal activity against Aspergillus multi, Aspergillus niger and Candida albicans. A compound exhibited low antibacterial and antifungal activity with the reference standard Streptomycin, Vancomycin and Nystatin respectively. The toxicity of the compound was also assayed via the determination of their LD50 value by using Dixon , s up and down method (1980). Studied compound was found to have an LD50 of 518.6 mg / kg of body weight.

The present study assessed the effects of O-anisidine hydrochloride and the ameliorating effect of Quercetin dihydrate in laboratory rats. Sixteen male and thirty two female rats (Rattus norvegicus) were used and divided into eight equal groups of two male and four female rats each. The results revealed that the treatment with Oanisidine hydrochloride for 30 days (T1, T2 and T3 groups) caused significant decrease in the males body weights, sperm count, individual and massive sperm motility, testes weights, and epididymis weights as compared with control and (T4, T5, T6 and T7) groups at (P≤0.05).When Quercetin dihydrate was offered as an ameliorating agent, it showed a significant ameliorating effect by increasing the body weights, sperm count, individual and massive sperm motility, testes weights, and epididymis weights . When Quercetin dihydrate was offered alone in the ration of the sixth treated group (T6), it caused clear significant ameliorating effect on all sperm parameters comparing with all treated groups and the sperm count was even significantly higher than that of control group while the other aspects were similar to those of control group at (P≤0.05). Beside, O-anisidine caused significant decrease in the number of pregnant females, number of delivered litters, weight of litters and sex ratio, and it prevented the pregnancy from being occurred in the group where both male and females are treated with it (T1). When Quercetin was mixed with Oanisidine in the ration of (T7), it significantly ameliorated the pregnancy chances,number of litters and the sex ratio as compared with the other groups but it didn’t reach to significant level with control group at (P≤0.05).