خيارات البحث
النتائج 391 - 400 من 553
Studies on nutrionally-related blood metabolites: total protein and glucose levels in goats of Kinta and Hilir Perak district النص الكامل
2011
Komala, T. S. | Mahadi, Y. | Khairunnisak, M. | Ramlan, M.
The objective of this study was to examine the nutritionally-related
blood metabolites; total protein and glucose levels in goats of Kinta and Hilir Perak districts. A total of eight farms were screened, involving a total of 220 animals. Serum samples were collected in plain tubes for total protein determination, while
plasma samples were collected in sodium fluoride tubes for glucose determination. Total protein and glucose determination were carried out using Idexx Vettest 8008 Blood Chemistry Analyzer. Results show
that goats in seven out of eight farms had malnutrition problems and poor body scores. Farmers were given advices based on the laboratory results obtained from this study.
اظهر المزيد [+] اقل [-]Isolation of Streptococcus equi during strangles surveillance in Peninsular Malaysia النص الكامل
2011
Yosnah Y. | Maswati M. A. | Saifu Nazri. R. | Khoo, l. l. | Roseliza R. | Ramlan M.
Strangles is an extremely contagious bacterial infection specific to
equine species( horses, mules and zebras). A nationwide screening of S. equi was conducted among horses following an isolation of Streptococcus equi subsp. equi (S. equi) from a horse. All horses were monitored for the presence of respiratory signs, nasal discharge and submandibular swelling. This paper reports the isolation of S. equi from horses during a nationwide survey from August 2010 to December 2010. From August 2010 to December 2010 our laboratory received 2,825 nasal swabs, 9 guttural pouch flushes, 1 submandibular swab and 1 submandibular abscess. The samples were subjected to conventional bacterial isolation and identification.
Streptococcus equi-positive samples were also confirmed by detecting the M-gene (SeM) of the bacteria by using PCR. Two nasal swabs from two horses and one submandibular abscess from a horse were positive for S. equi by culture and subsequently by PCR. Surveillance for S. equi should be continued for the control of the strangles. PCR can be carried out in parallel to bacterial culture to increase the
detection rate of carriers and shedders.
اظهر المزيد [+] اقل [-]Monitoring of melamine in milk and feed using ELISA and LCMS/MS screening methods النص الكامل
2011
Suhaimi D. | Lily Suhaida M. S. | Ismail M. | Wan Syahidah H.
A monitoring program for melamine in milk and feed was conducted in response to global melamine alertness in the year 2008. Two screening methods were adopted i.e., a liquid chromatography triple quadrupole tandem mass spectrometry (LC-MS/MS) and enzyme-linked
immunosorbent assay (ELISA). The liquid chromatography method developed by several international research centers was adapted. This method consisted of an initial extraction with 10%trichloroacetic acid (TCA) for milk samples or 60% methanol/water for feed samples, followed by a series of centrifugation, dilution and filtration steps. Melamine was analysed in the chromatographic program using a zwitterionic HILIC LC column. Electrospray ionisation in positive ion mode was used. The quantity of melamine
present was determined with a calibration curve consisting of sample extracts from milk or feed fortified from 25 to 50 ppb that were taken through the extraction procedure. The ranges of recovery from
fortified raw milk samples (n=20) and feed samples (n=21) was 70–80% and 68%, respectively. The limit of detection was estimated at 10 ppb for both matrixes. Milk samples were found negative for melamine,
however 4.5% of feed samples were found to contain the compound at concentrations between 1 to 5 ppm.
اظهر المزيد [+] اقل [-]Modulation of heat stress in buffaloes by supplementing electrolytes, ascorbate and zinc.
2011
Kumar, B.Y.Sunil | Singh, Gyanendra | Kumar, Ajeet | Kataria, Meena | Meur, S.K.
The ameliorative effect of salts, ascorbic acid olyphosphate and zinc oxide supplementation on heat stress was studied in adult buffaloes of either sex which were randomly divided into two groups of four animals each. Group I served as control and Group II was supplemented with sodium bicarbonate, potassium carbonate, ascorbic acid polyphosphate and zinc oxide. All the animals were exposed to two conditions of temperature and humidity: hot-dry and hot-humid in psychrometric chamber for 4 hours daily for 10 days. Blood was collected on days 1, 5 and 10 of treatment. The activities of enzymes catalase, superoxide dismutase (SOD) and concentrations of reduced glutathione (GSH), hormone cortisol and thiobarbituric acid (TBA) binding substances (lipid peroxidation) were estimated in serum. Significant decreasewas observed in activities of serum catalase and SOD. Serum GSH concentration decreased while serum concentrations of TBA binding substances and cortisol significantly increased in both groups which were subjected to heat stress. Dietary supplementation of ascorbate, zinc and electrolytes resulted in further decrease in the enzymes' activities where as the serum GSH increased at par to normal values. Serum concentrations ofTBA binding substances and cortisol were comparatively lower in supplemented group in both types of stress. Thus, supplementation of ascorbate and zinc in addition to electrolytes relieved the animals of oxidative stress.
اظهر المزيد [+] اقل [-]Prediction of body weight based on body measurements of pig.
2011
Kumar, Ravindra | Kuma, Ashok | Patel, M. | Kumar, Anil | Yadav, A. K.
Atotal of twenty four LargeWhite Yorkshireweaners nearly of same body weight were selected for the present study.The prediction equations were constructed to estimate the body weight of pigs indirectly from different body measurements of 24 pigs. The body weight was highly correlated (PO.OI)with body length, heart girth and height at withers. Accuracy for the measurement of body weight with prediction equation using one or two independent variables shifting from heart girth to height at withers with increase in body weight. Coefficient of multiple determination (R2)increases gradually as the number of independent variables increased in the prediction equation and the best prediction equation was observed when all the 3 body measurements were included in the equation. orrelationcoefficientof body weightwith body measurementswere also foundto be highly correlated.
اظهر المزيد [+] اقل [-]Management and treatment of idiopathic tetanus in a horse.
2011
Bhatt, P. | Shukla, Alok | Jadeja, V.S. | Gupta, D.K. | Dabas, Y.P.S.
A case of idiopathic tetanus in a horse was treated with large doses of long acting penicillins along with tetanus anti toxins, tetanus toxoid, muscle relaxants and metronidazole. Supportive medication included parenteral dextrose saline, antioxidants, nervine tonics and restoratives. The successful treatment ofthe case over a period of fifteen days was recorded.
اظهر المزيد [+] اقل [-]Immuno-pathological effect of Argemone maxicana in broilers (Gallus domesticus).
2011
Parihar, Shradba | Garg, U.K. | Shrivastava, Nidhi
An experiment was conducted on 100 day in old broiler chicks to study the immunosuppressive potential of Argemone mexicana seeds toxicity. Toxicity was induced by administrating crushed Argemone seeds @ 5, 10,20 and 30 grams per Kg of feed in treatment groups Tl, T2, T3 and T4 respectively whereas the Control group was maintained on the normal feed. The birds in the treatment groups showed the symptoms like dullness, depression and ruffled feathers. The body weight gain (BWG) was poor in comparison to control. The lymphoid organ to body weight ratio, HI titers and percentage of active macrophage were found significantly reduced. The histopathological lesions revealed marked lymphoid depletion indicaive of immunosuppressive potential ofArgemone seeds.
اظهر المزيد [+] اقل [-]Detection of InvA gene in isolated Salmonella isolates from spleen of pigs by Polymerase Chain Reaction (PCR).
2011
Chopade, N.A | Kurkure, N. V. | Narkhede, H. P. | Barbuddhe, S. B. | Bhandarkar, A.G. | Kalorey, D.R.
Nine Salmonella isolates (11.25%) from 80 spleen samples of pigs collected from slaughter house detected by conventional culturing when subjected to Salmonella-specific gene (invA) yielded product a 284-bp DNA fragment.
اظهر المزيد [+] اقل [-]Supplementation of Morinda citrifolia extract on growth, production and immune response of Japanese quail.
2011
Sunder, Jai | Singh, D.R. | Kundu, A. | Jeyakumarand, S. | Verma, S.K.
The response of Japanese quail to supplementation of Morinda citrifolia truit juice with reference to growth, production and immune response was studied. The Japanese quail were fedMorinda citrifolia ITuitjuice @5% from 0-7 weeks during growing period and from 8-14 weeks during laying periods. The body weight gains, feed conversion ratio, performance index and immunity status were recorded in both control and treated groups. The result revealed better growth performance in theMorinda fed groups compared to the control group. Overall performance index of Morinda group was also better than control group. The humonil immune response ofthe Morinda group was significantly better than control group. The Morinda fed birds produced 30.90 eggs / hen housed during 8-14 weeks of age. While only 20.9 eggs/hen housed produced by control groups. The overall analysis of the growth and production performance of both the groups revealed that supplementation of M. citrifolia crude truit juice @ 5% daily enhanced the body weight gain and egg production performance in the Japanese quail.
اظهر المزيد [+] اقل [-]Effect of different levels of organic chromium on egg yolk cholesterol and chromium contents.
2011
Palod, Jyoti | Kumar, A. | Singh, V.S. | Shukia, P.K.
Afeeding trial was conducted in layers for a period of8 weeks (23-30 weeks old) to evaluate the effect of chromium picolinate supplementation on egg yolk cholesterol and egg chromium content in layers.Atotal of (n=120) layers were randomly distributed in completely randomized design into 4 treatmentgroupseachwith2 replicates of 5chicks.The layers of treatment group T1(control)were provided water without chromiumwhile those of T2,T3andT4were provided water containing 200, 400 and 600 ppb chromium / litre respectively.At the end of feeding trial, six eggs from each replicate, on last three days were used for egg yolk cholesterol and egg chromium studies. The results indicated that the total cholesterol andLDL cholesterol content of eggs were significantly (P0.05) reduced and HDL-cholesterol content significantly increased in 400 and 600 ppb chromium supplemented group of layers. Whereas chromium content of eggs increased with increasing levels of chromium supplementation. It was concluded that organic chromium supplementation up to 600 ppb level through water may be advisable to reduce egg yolk total cholesterol and LDL- cholesterol and improved HDL-cholesterol and egg chromium content.
اظهر المزيد [+] اقل [-]