These studies were carried out to investigate the changes of blood pictures, histological findings and testosterone concentrations after orchidectomy in ageing rat. The total W.B.C. counts were 9.27 +- 12 x 10** (3)/mm** (3) in the control group, 9.54 +- 34 x 10** (3)/mm** (3) in the sham-operation group and 12.08 +- 28 x 10** (3)/mm** (3) in the orchidectomy group when it was checked 50days after the operation. The number of lymphocytes after orchidectomy began to increase gradually as time passes, thereafter markedly increased 10.12 +- 03 x 10** (3)/mm** (3) at 50 days after orchidectomy. The concentration of testosterone in serum began to decrease gradually after orchidectomy showed lowest level 1.68 +- 0.61 pg/ml at 50 days after orchidectomy while it was 5.82 +- 0.39 pg/ml in control group. The histological findings of thymus after orchidectomy marked increase of thymic lymphocyte, disapearance of fat cells and appearance of mitotic figures

In present study the transtracheal aspiration technique using a commercial polypropylene 5 fr. urinary catheter with 13-gauge 2.5 inch hypodermic needle was applied to bovine medicine in order to determine the availability of this procedure for diagnosis of lower respiratory tract disease. The subjects were forty adult cattle which consisted of thirty-one cows with respiratory signs such as cough and nasal discharge and nine cows without these signs. This procedure appeared suitable for collecting specimens of the lower respiratory tract secretions without uncontamination by the flora of the mouth, respiratory distress and any complications. The most of microorganisms isolated was Pasteurella multocida (48.7 %) and a mucopurulent inflammatory response was the most appeared accounting for 40 % of cytological basis. Most cows with severe clinical signs showed a mucopurulent inflammation (60 %) and Pasteurella multocida were isolated dominantly in severe cases (63.2 %). Cows with mild clinical signs were distributed in each of the five cytological classification and Pasteurella multocida was the most isolated accounting for 40 % of mild cases. In the cytologic examination, a few neutrophils and epithelial cells were seen in the fluid of mucoid inflammation, and many neutrophils with epithelial cells and thick mucoid exudate were seen in the fluid of mucopurulent inflammation. Various number of neutrophils, lymphocytes, macrophages and epithlial cells were examined in the fluid of mixed cell inflammation. The columnar ciliated epithelial cells were examined mainly in normal aspirates. From these results the transtracheal aspiration technique as a diagnostic aid for lower respiratory tract disease of cow is considered as a simple, safe and useful method and can be performed in the field situation

In order to manifest the presence of Na-K pump and its property on the unfertilized egg membranes of mouse, membrane potential was recorded under the physiological condition (at 37deg C and 4mM Ca** 2+). After an induction of superovulation, the fresh eggs with zona pellucida were collected from mouse oviduct. Transient hyperpolarization as pump action was recorded after the switch into the high potassium perfusate (15mM K+) from K-free perfusate, and the difference between membrane potential observed just before the perfusion of high potassium solution and the maximal membrane potential during the perfusion of high potassium solution was regard as pump activities. Resting membrane potential was depolarized under the treatment of 10** (-5) ouabain. Pump activities of the unfertilized mouse eggs were -3.38 +- 0.61mV (Mean +- SD, n=6), recorded as transient hyperpolarization due to the electrogenic property. Pump activities were blocked by both treatment of 10** (-5)M ouabain and perfusion of Na-free solution, while increased by high Na + (300mM) perfusion (-7.45 +- 0.75mV, n=2). Hyperpolarization due to pump activity was not altered by Mn 2+. Above results confirm the presence of ouabain-sensitive Na-K pump, which affected the membrane potential directly, on the unfertilized egg membranes of mouse

Hematologic values were determined in blood samples obtained from 203 normal, healthy Jindo dogs of both sexes that ranged in age from 3 months to 10 years. Age-related differences were detected for the total erythrocyte count, hemoglobin content, PCV and the number of eosinophils. The persistent eosinophilia appears to be due to the dirofilariasis and flea-allergy dermatitis

In order to measure in vitro cell-mediated immunity in the guinea pigs sensitized with the killed bacilli of Mycobacterium bovis (AN5), M avium (serotype 2), M tuberculosis and M intracellulare (serotype 8), leukocyte adherence inhibition (LAI) test was established using the antigens of purified protein derivatives (PPD) tuberculin. By using LAI test, specificity of cell-mediated immune responses of the guinea pigs inoculated with various Mycobacterium spp was investigated, and comparison between values of LAI and skin test was also made to evaluate the specificity of the newly designed test. The optimal concentration of PPD antigens for LAI test was 1 to 2mg per ml of medium. When the leukocytes of guinea pigs sensitized with both M bovis (AN5) and M avium (serotype 2) for 2 to 8 weeks were incubated with homologous or heterologous PPD antigens, mean values of LAI test were 61.2 +- 11.2 and 65.6 +- 5.1 % in homologous PPD antigens respectively, while 30.0 +- 3.7 and 32.8 +- 5.7 % in heterologous PPD antigens, showing the prominently high value of LAI in the homologous system (p0.01). When the leukocytes of guinea pigs sensitized with both M tuberculosis and M intracellulare (serotype 8) for 2 to 8 weeks were incubated with homologous and heterologous PPD antigens, mean values of LAI test were 67.9 +- 2.9 and 66.9 +- 5.0 % in homologous PPD antigens, while 27.4 +- 7.4 and 24.4 +- 7.1 % in heterologous PPD antigens, showing the prominently high value of LAI in the homologous system (p0.01). Comparing with the specificity of LAI and skin tests on the basis of the value obtained from the homologous system, deviation of reaction was revealed to be 49.5 to 100.2 in LAI test, and -15,9 to 52.0 in skin test

This work was carried out to get some informations about blood types and their researches, involved blood stock and genetic identification. Horses examined were total 55 heads of sire, mare and their progeny in Korean Horse affairs Association. Albumin and phenotypes of 26 mare were examined. The appearance of phenotype AA, BB, AB, was 1, 18, 7 respectively. The gene frequency of albumin A was 0.17 and albumin B was 0.76. The appearance of phenotype AA, BB, Ab in 29 progeny was 1, 16, 12 respectively. The gene frequency of albumin A was 0.24 and albumin B was 0.76. The gene frequency of gene A was higher than their parents. Identification of the relationship between parents and their progeny was also examined. 4 of type AB between AA and BB, 4 of type BB between BB and BB, 13 of type AB between BB and AB were borned. In third case, all of progeny was type AB. This results suggest positive relationship between them

In order to establish the extent of Streptococcal arthritis piglets, isolation of Streptococci from arthritic lesions of 34 piglets were undertaken from November 1987 to October 1988 in Korea. Also determined were isolation frequency of Streptococci in nasal cavity of 250 healthy sows and antibiotic susceptibilities of the isolates. Streptococci were isolated from 52.9 % of 34 arthritic piglets and 20 strains isolated belonged to 4 S suis type I, 8 S suis type II, 2 Lancefield group C and 6 group E. From 28.8 % of 250 healthy sows, 72 strains of Streptococci were isolated and these consisted of 9 S suis type I, 51 S suis type II and 12 group C. Streptococcal arthritis seemed to occur prominently in piglets aged 2 to 4 weeks and in male than female. No significant difference were recognized in tarsal and carpal joints as affecting site. All of 92 isolates were sensitive to ampicillin and penicillin, and all strains of S suis type I and group E Streptococcus were also sensitive to chloramphenicol and cephalothin. To cephalothin all strains of group C Streptococcus were sensitive. The 1.7 to 100 % of 92 isolates were resistant with different prevalence to colistin, erythromycin, kanamycin, tetracycline, gentamycin, chloramphenicol and cephalothin. The 92.5 % of these resistant Streptococci were multiply drug-resistant strains. The drug resistant patterns most frequently encountered were Tc Cl Em Km Gm (16.3 %) in quintuple pattern, Tc Cl Em Km (16.3 %) in quadruple pattern, Tc Cl Em (10.9 %) in triple pattern and Cl Em (14.1 %) in double pattern

The capsular serogroupes and drug susceptibility of 111 isolates of Pasteurella multocida from pigs with atrophic rhinitis and pneumonic lesions were investigated. Of the 111 P multocida isolates, 42 were from lung lesions, 47 from nasal turbinate lesions and the remaining 22 from the nasal swabs. P multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavine auto-agglutination. Most isolates (64.9 %) were type A, 23.4 % were type D and the remaining 11.7 % were untypable. Resistance to triple sulfa (97.3 %) was most frequent, followed by resistance to tiamulin (71.2 %), tylosin (56.8 %), streptomycin (36.9 %), and neomycin (36.0 %). The majority of the organisms were susceptible in order of prevalence to baytril (100 %), ampicillin (98.2 %), linsmycin (97.3 %), cephalothin (94.6 %), gentamycin (93.7 %), amikacin (92.3 %), tetracycline (91.9 %), trimethoprim/sulfamethoxazole (91.0 %), and kanamycin (90.1 %). No differneces in drug resistance in relation to capsular serogroupes of P multocida and the origin of lesions were noted. A high prevalence of multiple drug resistance was observed and the most common resistant patterns were Sss, Tm, Ty (12.6 %) and Sm, Sss, Tm, Ty (8.1 %) patterns

This experiment was carried out to investigate the hematogical and blood chemical values in dairy cattle during the dry and lactating period. Blood was collected from six healthy dairy cattle in Kyongnam province. Leucocytes count, hemoglobin concentrations and the percentage of packed cell volume were lowest at the early lactation period. Leucocytes count was not affected during dry to lactating period. The differential count of eosinophilic leucocytes was low at the late lactation period, while the basophilic leucocytes was high at the dry period. Inorganic phosphorus value was below under normal leve. Creatinine value was from 1 to 2mg/100ml of serum. Positive reaction to CRP was shown in normal dairy cattle. The values of AST and ALT were higher during the dry than lactating period. r-GTP and total cholesterol tend to be decreased during the lactating period. CPK value was not affected for dry and lactating period

A new sudden death in rabbits appeared in China and Korea in 1984 and 1985, respectively, and was recognized to be an acute infectious disease caused by a virus. The disease was reported as a "new viral disease", and thereafter, a tentative name of "viral hemorrhagic disease", "hemorrhagic pneumonia" or "viral hemorrhagic pneumonia" has been described in the case reports. But authors had called the viral disease "rabbit viral hepatitis" due to picornavirus infection, because the principal lesion of the disease was an acute hepatitis. The purpose of this report is to describe the electron microscopic findings on the livers in experimentally infected rabbits. All the livers of the affected rabbits were shown to have degenerative changes of a type that is characteristic of acute hepatitis. In the liver cells, there were dilation of rER and mitochondria, vacuole formation of various sizes, and appearances of many virus-like particles in the vicinity of rER, granular bodies and crystalline arrays of viral particles in the cytoplasm with necrotic changes of the nucleus. Clusters of virus-like particles and viral crystals appeared in the cytoplasm of sinusoid endothelial cells and Kupffer's cells with morphological changes of organelles. Also viral crystals were demonstrated in the cytoplasm of macrophages among the liver cells. On the whole, the liver cells had many virus-like particles and a few crystalline arrays of viral particles. Therefore, this implies that the liver cells are the main site of the viral replication in inducing the viremia. It was concluded that the liver was the primary target organ of this viral disease, and the pathological and the ultrastructural evidence suggest that the virus may belong to genus enterovirus