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Propagation of avian influenza virus in embryonated ostrich eggs النص الكامل
2022
Agnes T. Laleye | Modupeore Adeyemi | Celia Abolnik
Propagation of avian influenza virus in embryonated ostrich eggs النص الكامل
2022
Agnes T. Laleye | Modupeore Adeyemi | Celia Abolnik
Influenza A viruses (IAVs) are typically isolated and cultured by successive passages using 9- to 11-day-old embryonated chicken eggs (ECEs) and in 14-day old ECEs for virus mutational studies. Real-time reverse transcription-polymerase chain reaction tests (RT-PCRs) are commonly used for IAV diagnosis, but virus isolation remains invaluable in terms of its high sensitivity, providing viable isolates for further studies and the ability to distinguish between viable and nonviable virus. Efforts at isolating ostrich-origin IAVs from RT-PCR positive specimens using ECEs have often been unsuccessful, raising the possibility of a species bottleneck, whereby ostrich-adapted IAVs may not readily infect and replicate in ECEs, yet the capacity of an ostrich embryo to support the replication of influenza viruses has not been previously demonstrated. This study describes an optimised method for H5 and H7 subtype IAV isolation and propagation in 28-day old embryonated ostrich eggs (EOEs), the biological equivalent of 14-day old ECEs. The viability of EOEs transported from breeding sites could be maximised by pre-incubating the eggs for 12 to 14 days prior to long-distance transportation. This method applied to studies for ostrich-adapted virus isolation and in ovo studies will enable better understanding of the virus-host interaction in ostriches and the emergence of potentially zoonotic diseases.
اظهر المزيد [+] اقل [-]Propagation of avian influenza virus in embryonated ostrich eggs النص الكامل
2022
Laleye,Agnes T. | Adeyemi,Modupeore | Abolnik,Celia
Influenza A viruses (IAVs) are typically isolated and cultured by successive passages using 9- to 11-day-old embryonated chicken eggs (ECEs) and in 14-day old ECEs for virus mutational studies. Real-time reverse transcription-polymerase chain reaction tests (RT-PCRs) are commonly used for IAV diagnosis, but virus isolation remains invaluable in terms of its high sensitivity, providing viable isolates for further studies and the ability to distinguish between viable and nonviable virus. Efforts at isolating ostrich-origin IAVs from RT-PCR positive specimens using ECEs have often been unsuccessful, raising the possibility of a species bottleneck, whereby ostrich-adapted IAVs may not readily infect and replicate in ECEs, yet the capacity of an ostrich embryo to support the replication of influenza viruses has not been previously demonstrated. This study describes an optimised method for H5 and H7 subtype IAV isolation and propagation in 28-day old embryonated ostrich eggs (EOEs), the biological equivalent of 14-day old ECEs. The viability of EOEs transported from breeding sites could be maximised by pre-incubating the eggs for 12 to 14 days prior to long-distance transportation. This method applied to studies for ostrich-adapted virus isolation and in ovo studies will enable better understanding of the virus-host interaction in ostriches and the emergence of potentially zoonotic diseases.
اظهر المزيد [+] اقل [-]Comparative effect of dose escalation of nanocapsulated ivermectin against mange in rabbits النص الكامل
2022
Azza Abdelmoteleb | Dalia Elmasry | Fatma Amro | Reham Mahmoud
The present study aimed to investigate the effects of nano-capsulated ivermectin on the liver and kidney function and oxidative status in mite infested-rabbits, compared to ivermectin. Additionally, the ivermectin residue profile in adipose tissue, liver, muscle, and kidney was evaluated. For this purpose, nano-capsulated ivermectin was prepared and characterized using high-resolution transmission electron microscopy (HRTEM) and cytotoxicity assay on Vero cells. To assess the effect of dose escalation of nano-capsulated ivermectin, one-hundred naturally mite-infested male rabbits were divided into four groups (G1-G4; n=25). Rabbits kept in G1 were left untreated (positive control), while rabbits kept in G2 and G3 received subcutaneously 200 and 400 μg/kg body weight ivermectin, respectively, at zero-day and repeated after two weeks of the first injection. Rabbits in G4 were treated with 200 μg/kg nano-capsulated ivermectin at zero day as a single dose. Additionally, twenty-five healthy male rabbits (G0) were used as a negative control. The efficacy was assessed based on clinical manifestations, liver and kidney function, and oxidative stress parameters. Ivermectin residues were measured in fat, liver, muscle, and kidney using high-performance liquid chromatography (HPLC). Results showed that the size of the nano-capsulated ivermectin was 35.4 nm with a narrow size distribution of 0.578 polydispersity indexes. A significant improvement in liver and kidney functions (P
اظهر المزيد [+] اقل [-]Bovine Brucellosis serological survey in small dairy herds in Lushnja district, Albania النص الكامل
2022
Arla Juma | Garald Muça | Anita Koni | Luigj Turmalaj | Xhelil Koleci
Background: Bovine brucellosis is an important zoonotic disease in Albania. Both, Brucella abortus and B. melitensis have been isolated from ruminants. National control and eradication programs for brucellosis are applied in sheep and goat farms as well as on larger dairy cattle farms. A study was performed in beef cattle farms, to assess herds and within herd prevalence. The prevalence of bovine brucellosis in small dairy herds was unknown and its importance has been largely neglected. The current study aimed to assess the herd and within herd prevalence of bovine brucellosis in small bovine herds and to provide scientific evidence for establishing an evidence-based approach to control the disease in this subset of the population not previously included in the national eradication program. To achieve this objective, a statistical survey was designed and implemented in small dairy herds in the Lushnja district where samples from statistically selected herds were serologically tested in parallel with Rose Bengal Test (RBT), Fluorescence Polarization Assay (FPA) and Enzyme-Linked Immunosorbent Assay (ELISA). Methodology: In total, 120 dairy herds were randomly selected from a list of 1,955 registered herds: from these selected herds 368 blood samples were collected from all animals older than 12 months, and their sera were tested using RBT, FPA, and c-ELISA. Results: The test results revealed no positive or suspect cases. Based on these results, we are confident (P ≤ 0.05) that currently in the Lushja district, Brucella spp is not circulating in this subpopulation of cattle. This deduction is supported by analyses of the main risk factors, other epidemiological data and the perceptions of official and private veterinarians. Conclusion: This is the first structured survey of bovine brucellosis in small dairy herds in Albania. In conclusion, our study results and our findings show that the epidemiological status of bovine brucellosis in the Lushnja district is encouraging. A test and slaughter control program appears to be appropriate in smaller herds. The approach used in this pilot study could be extended to establish the prevalence of brucellosis in other districts, the result of which would establish the basis for rational control measures in the smaller herds of cattle.
اظهر المزيد [+] اقل [-]Investigation of an outbreak of brucellosis in a dairy mixed farm and evaluation of a proper test and slaughter strategy to release the herd out of the quarantine النص الكامل
2022
Mohamed El-Diasty | Khaled tohfa | Fatma El-Hofy | Ashraf Tawab | Enas Soliman
An outbreak of brucellosis in a dairy mixed farm with a total of 508 animals at Fayoum governorate, North Upper Egypt was investigated. The present study showed that extensive animal farming was a potential risk factor for interspecies transmission of brucellosis. The seroprevalences of brucellosis using Rose Bengal Test (RBT) and Buffered Acidified Plate Antigen Test (BAPAT) were (9.5%) in cattle, (35%) in sheep, and (50%) in camels. The milk ring test (MRT) identified fewer positive cases than BAPAT and RBPT due to less sensitivity to detect low concentrations of antibodies in milk or due to fat clustering factors. Therefore, MRT cannot be used alone to get rid of the infection inside the farm. A total of 31 Brucella isolates were recovered from cows and sheep on the farm. Bacteriological examination and molecular confirmation of isolated Brucella species using AMOS-PCR confirmed that all isolates were typed as Brucella melitensis biovar 3. AMOS-PCR was a perfect method for rapid, sensitive, and accurate Brucella detection at the species level. The strategy of test and slaughter has been applied to eradicate brucellosis from the farm. The early release of the herd out of the quarantine should be avoided especially under unhygienic conditions and lack of controlled movement of animals. However, the animal population must be subjected to successive serological examinations for the exploration of animals that may be still incubating the disease. Despite 50% of male camels being seropositive, no clinical signs have been reported. Mixed breeding systems have to be avoided and the application of biosecurity practices as well fair compensation policy for owners should be implemented.
اظهر المزيد [+] اقل [-]Immune response and recent advances in diagnosis and control of brucellosis النص الكامل
2022
Alyaa Elrashedy | Mohamed Gaafar | Walid Mousa | Mohamed Nayel | Akram Salama | Ahmed Zaghawa | Ahmed Elsify | Ali Dawood
Brucellosis is a zoonotic disease that has serious animal welfare and economic consequences worldwide. In mammals, this stealthy intracellular pathogen causes abortion and infertility, and in humans, it produces a terrible febrile illness that can progress into a long-term condition with serious implications. The pathogenicity of brucellae is based on their ability to survive and replicate in host cells, which allows them to escape from the immune system. The gold standard test for diagnosis, which demands competence, is still isolation and identification. Advancements in diagnostic procedures and screening of recently infected animals are required to achieve effective control. Despite their drawbacks, the most widely used vaccine strains to protect against Brucella infection and relevant abortions in cattle are B. abortus strains S19 and RB51 and in small ruminants is B. melitensis Rev1. However, there are no safe vaccine candidates for humans. Therefore, it is critical needs to improve vaccine production using advanced techniques such as subunit vaccines that are both effective and safe. Studying the overview of the Brucella immune response mechanism and advances in the diagnosis procedures allow more understanding of effective control strategies. The current review provides an overview on the immune response and updates on the diagnosis and control of brucellosis based on published literature on different search engines
اظهر المزيد [+] اقل [-]The objectivity of Vertebral Heart Scale: Comparison of Detailed Radiographic Findings with Vertebral Heart Scale in Symptomatic Dogs with Cardiac Failure النص الكامل
2022
Sinem Saka | Onur İskefli | Mehmet Or | Utku Bakırel
The current study aimed to evaluate the objectivity of the vertebral heart scale in the right, left, and generalized cardiomegaly. The correlation between vertebral heart scale and cardiomegaly was determined by conventional methods using a detailed evaluation form of vertebral heart scale and main stem bronchus spine distance measurements and radiographic findings. For this reason, a detailed radiographic evaluation form, including cardiac shape alterations, tracheal displacement, the ratio of the width of the heart to the thorax, and effusive findings due to cardiac failure, was compiled to provide gradation and detection of both-sided alterations in latero-lateral and dorso-ventral view. X-rays of 189 symptomatic dogs with cardiac disorders were included in the study and evaluated by experienced staff. X-ray findings were classified as left-right and both-sided enlargement and graded as mild, moderate, or severe enlargement. Using statistical analysis, the vertebral heart scale method was found to be correlated with findings of left and generalized enlargement, and a significant difference was found between the moderate and severe class (respectively P
اظهر المزيد [+] اقل [-]Salmonella sp. and Staphylococcus aureus contamination on food and hands of food handlers at Food Management Sites (TPM) Ulee Lheue Seaport النص الكامل
2022
Nurliana, Nurliana | Raudhah, Raudhah | Ferasyi, Teuku Reza | Sugito, Sugito | Darmawi, Darmawi | Sari, Wahyu Eka
This study aims to determine the presence of Salmonella sp. and Staphylococcus aureus bacteria in rendang meat, fried chicken, fried fish, and omelets, as well as in the hands of food handlers in the Food Management Place (TPM). Ulee Lheue Sea Port by using laboratory tests. The study used five samples of food and five samples of food handlers' hands taken from the Food Management Place (TPM) of Ulee Lheue Seaport. Bacterial analysis on food samples in the laboratory using the Total Plate Count (TPC) method. Meanwhile, the Replicate Organism Direct Agar Contact (RODAC) method was used to examine food handlers' hands. Laboratory tests resulted from five food samples and five food handlers' hand samples that Salmonella sp. was found in omelets, and Staphylococcus aureus was found in beef rendang, fried chicken, fried fish, omelets, and hand samples of food handlers. It is necessary to conduct hygiene and environmental sanitation counseling at TPM around Port of Ulee Lheue.
اظهر المزيد [+] اقل [-]Evaluating of The Addition Coconut Pulp (Cocos Nucifera L) Fermentation in Feed and The Effect on Percentage of Carcass Broiler Chicken النص الكامل
2022
Ayuti, Siti Rani | Fikri, M. | Rastina, Rastina | Herrialfian, Herrialfian | Helmi, T. Zahrial | Isa, M. | Zamzami, Rumi Sahara
Broilers are chickens with high meat production. The productivity of broiler is seen from the percentage of carcass and abdominal fat. This study aims to determine the effect of giving fermented coconut pulp (Cocos nucifera L) on the percentage of carcass and abdominal fat of broilers. This study used a completely randomized design experimental method (CRD) consisting of 6 treatments and 6 replications so that the total observations were 36 units of observation. Each treatment had P1: 100% commercial feed without fermented coconut dregs, P2: 90% commercial feed + 10% fermented coconut dregs, P3: 80% commercial feed + 20% fermented coconut dregs, P4: 70% commercial feed + 30 % fermented coconut dregs, P5: 60% commercial feed + 40% fermented coconut dregs, P6 : 50% commercial feed + 50% fermented coconut dregs. The data were analyzed using the analysis of variance which was preceded by the battle test, followed by the Tukey test using the SPSS program. The analysis of variance showed significantly different results (P0.05) on the percentage of carcass and abdominal fat of the broiler. Tukey's follow-up test on the carcass showed a significant difference (P0.05) and there was no significant difference in fat (P0.05). It can be concluded that the supplementary feed of fermented coconut pulp can be used 40% as additional feed to increase the carcass percentage but not reduce the broiler abdominal fat percentage.
اظهر المزيد [+] اقل [-]Knowledge, Attitudes and Actions of Farmers on Qanun Number 3 of 2016 About Controlling Productive Female Cattle and Buffalo in Aceh Besar District النص الكامل
2022
Nugraha, Satria | Ferasyi, Teuku Reza | Nurliana, Nurliana | Sugito, Sugito | Helmi, Teuku Zahrial
The research was at determining the knowledge, attitudes, and actions of farmers on the Qanun number 3 of 2016 regarding the control of productive cows and buffaloes to avoid slaughtering productive cows and buffaloes so that livestock populations are maintained. This research was conducted using a survey method for three months starting from May 2021 to July 2021. Breeders were selected using the purposive sampling method and interviews were conducted by filling out a validated questionnaire. The data analysis method used was descriptive qualitative analysis using a structured questionnaire to 110 farmer respondents with the criteria of having 2 years of experience in raising cattle, and raising female cows. The livestock population has increased since Qanun Number 3 was issued in 2016. The results of this study indicate that the level of knowledge of farmers is in a good category (72.5%), attitude is a very good category (83.2%) and action is good category (72.5%).
اظهر المزيد [+] اقل [-]Detection of Beef Type Dark, Firm, and Dry (DFD) and Pale, Soft, and Exudative (PSE) for Sale at Peunayong Market Banda Aceh النص الكامل
2022
Novita, Andi Novita | Siregar, Silvia Rahman | Ferasyi, Teuku Reza
This study aims to determine beef types Dark, Firm, and Dry (DFD) and Pale, Soft, and Exudative (PSE) that are sold at the Peunayong Market, Banda Aceh. A total of nine samples of beef from different sellers will be used as extracts and the pH is measured three times every 1 hour for 12 hours to determine the trend of decreasing pH. Organoleptic observations were carried out to determine the visual differences shown by the meat samples. Parameters observed were meat color, meat aroma, meat texture and surface state of the meat. The data obtained from pH measurements and organoleptic tests were analyzed descriptively. Based on the results of pH measurements seen from the decreasing trend of pH in nine samples of beef, seven of them were PSE type beef with a pH value of 5.2 and the other two samples were of good quality meat with a pH value ranging from 5.6 to 5.4. From the results of organoleptic tests for color parameters, four samples were pale, one sample was very pale and the rest were red. All the meat samples tasted normal. Meat texture and surface condition for soft-textured meat has a wet surface, while hard-textured meat has a dry surface.
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