Cardiovascular consequences of butorphanol tartrate (0.2 mg/kg of body weight, IV) administration during isoflurane (1.7% end-tidal concentration) anesthesia were determined in mechanically ventilated healthy dogs. Butorphanol administration caused significant (P less than or equal to 0.05) reductions in mean, systolic, and diastolic arterial blood pressures; cardiac output; and rate-pressure product.

Twenty-one mixed-breed pony foals, reared and maintained under parasite-free conditions, were used to test the efficacy of ivermectin in oral drench and paste formulations (200 microgram/kg) against 11-day-old migrating larvae of Parascaris equorum. Three replicates of 4 foals and 3 replicates of 3 foals were formed on the basis of age. Foals in replicates of 4 were randomly allocated to be indicators, or to receive vehicle (control) or ivermectin paste or ivermectin liquid. Foals in replicates of 3 were randomly allocated to receive vehicle or ivermectin paste or ivermectin liquid. The recovery of larvae from the lungs, liver, and small intestines of the indicator foals showed that 99.9% of the larvae were in the lungs 11 days after inoculation (day 0 of treatment). The recoveries of larvae from lungs and small intestines of controls at 25 days after inoculation indicated that all larvae had migrated to the small intestine by this time. The mean length of larvae recovered from the lungs (11 days after inoculation) was 0.87 mm; the mean length of those recovered from the small intestine (25 days after inoculation) was 3.65 mm. Using larvae recovered from small intestinal contents for calculations, ivermectin in both formulations was 100% effective against 11-day P equorum (P less than 0.01, compared with control group geometric mean of 1498.4).

Sixteen 7-week-old Holstein male calves were inoculated with sporulated oocysts of Eimeria zuernii. Four calves (controls) were euthanatized and necropsied at 14 and 20 days after inoculation (DAI). Two calves were treated with 20 mg of dexamethasone (IM) on 13, 14, and 15 DAI and euthanatized and necropsied 17 DAI and 2 calves were given similar treatments and necropsied 20 DAI. The 8 other calves were euthanatized and necropsied 20 DAI. Two were started on the anticoccidial drug decoquinate in feed 13 DAI; 2 others were given decoquinated on the same schedule plus dexamethasone on 13, 14, and 15 DAI. Two calves were given the antibiotic narasin in feed beginning 13 DAI and 2 calves were given parasin on the same schedule plus dexamethasone on 13, 14, and 15 DAI. All calves, except 2 controls necropsied 14 DAI and 4 calves given decoquinate, discharged moderate-to-large numbers of oocysts in feces and had moderate-to-severe changes in fecal consistency. Histologic examintions revealed large numbers of endogenous stages in tissues of calves treated or not treated with dexamethasone. Few endogenous stages were observed in tissues from calves that were given decoquinate or decoquinate plus dexamethasone. Calves given narasin or narasin plus dexamethasone had moderate-to-large numbers of endogenous stages in the tissues.

Anthelmintic efficacy of levamisole against induced infections with 7- and 21-day-old Haemonchus contortus, Ostertagia circumcincta, Trichostrongylus axei, and T colubriformis was evaluated as an oral drench in goats. Group 1 (n = 8) was not treated, group 2 (n = 8) was given 3.96 mg of levamisole/kg of body weight, group 3 (n = 8) was given 7.92 mg of levamisole/kg, and group 3 (n = 7) was given 11.88 mg of levamisole/kg. Efficacy against all worms was low in goats given 3.96 mg of levamisole/kg, but was high against adult H contortus (99%) and adult T colubriformis (99.7%) in goats given 7.92 mg of levamisole/kg. Although efficacy against adults of all species was high in goats given 11.88 mg of levamisole/kg, some immature worms of all species remained in the abomasa of goats.

Seven hybridomas that secreted monoclonal antibodies (MAB) against the peplomer protein and one that secreted MAB against the nucleocapsid protein of Berne virus (proposed family Toroviridae) were isolated. All MAB directed against the peplomer protein neutralized virus infectivity and, with the exception of MAB 6A7, inhibited each other's binding in competition assays. Neutralization of Berne virus infectivity was potentiated when some MAB were used in pairs. The antibodies have been used to localize toroviral proteins in infected cells; use of antipeplomer MAB 6B10 yielded a diffuse intracytoplasmic immunofluorescence, whereas the antinucleocapsid MAB 1F1 detected antigen in the intra- and perinuclear compartments. By use of radioimmune precipitation, protein A of Staphylococcus aureus was found to bind directly to the nucleocapsid polypeptide, without the requirement for specific antibody. Using fluorescein isothiocyanate-conjugated protein A, the intranuclear accumulation of the nucleoprotein of Berne virus was confirmed by results of immunofluorescence.

This study was carried out to investigate the origin, insertion, direction of muscle fibers and structure of the ear muscles of the Korean native goat. The description was based on the dissection of fifteen Korean native goats with embalming fluid. The ear muscles of the Korean native goat were composed of the Musculus zygomaticoauricularis, M. scutuloauricularis superficialis, M. scutuloauricularis profundus, M. frontoscutularis, M. interscutularis, M. parietoauricularis, M. cervicoscutularis, M. cervicoauricularis superficialis, M. cervicoauricularis medius, M. cervicoauricularis profundus, M. auricularis profundus posterior and M. parotidoauricularis. The M. frontoscutularis clearly seperated into temporal and frontal parts in 6 cases. The M. scutuloauricularis profundus clearly separated into major and minor parts. The M. zygomaticoauricularis blended with the M. parotidoauricularis near its insertion, but not with the M. scutuloauricularis.

The present study was conducted in order to get the normal blood chemical values of Korean Jindo dogs. Blood samples were taken from 160 (male 34, female 126) healthy Jindo dogs in Jindo area. The mean values of serum total protein (TP), albumin (Alb) and globulin (Glb) content, cholesterol (Chole), magnesium (Mg), calcium (Ca), inorganic phosphate (P), potassium (K), sodium (Na) and chloride (Cl) concentration in the group of less than one year old were 6.64 (male 6.62, female 6.64), 3.63 (male 3.57, female 3.65) and 3.00 (male 3.05, female 2.99) g/100ml, 170.97 (male 166.46, female 172.68) mg/100ml, 1.45 (male 1.43, female 1.46), 5.76 (male 5.62, female 5.81), 4.80 (male 4.95, female 4.75), 4.84 (male 4.72, female 4.89), 148.93 (male 148.79, female 148.98) and 110.22 (male 110.42, female 110.14) mEq/L, respectively, whereas the TP, Alb and Glb content, Chole Mg, Ca, P, K, Na and Cl concentration in the group of one year old and more were 6.88 (male 6.84, female 6.89), 3.65 (male 3.63, female 3.66) and 3.23 (male 3.21, female 3.23) g/100ml, 167.48 (male 173.80, female 166.48) mg/100ml, 1.40 (male 1.36, female 1.40), 5.69 (male 5.53, female 5.71), 4.62 (male 4.73, female 4.60), 4.88 (male 4.90, female 4.87), 149.86 (male 149.60, female 149.90)and 110.03 (male 110.70, female 109.92) mEq/L, respectively. The ratios of mean serum albumin to globulin (A/G), calcium to inorganic phosphate (Ca/P) and sodium to potassium (Na/K) in the group of less than one year old were 1.21 (male 1.17, female 1.22), 1.20 (male 1.14, female 1.22) and 30.77 (male 31.52, female 30.47), respectively, whereas the A/G, Ca/P and Na/K in the group of one year old and more were 1.13 (male 1.13, female 1.13), 1.23 (male 1.17, female 1.24) and 30.71 (male 30.53, female 30.78), respectively. The mean values of Alb content, Mg, Ca and K concentration, A/G and Ca/P ratio appeared to be higher in the female than in the male, whereas the reverse was the case with P concentration. No differences were found between male and female in the TP and Glb content, Chole, Na and Cl concentration and Na/K ratio.

Effect of raising types and environmental conditions on the infection of Toxoplasma in the swine, the cat and the man were studied in Cheju Island from Sept. 1987 to Aug. 1988. Blood samples were taken from 214 conventionally raised swine in 6 villages and 506 swine raised in swine specialized farms, 122 cats raised under free moving or restrained conditions in 8 locations, 113 butchers, and 210 villagers. Toxoplasma antibody values of the blood sera were determined using the enzymelinked immunosorbent assay (ELISA). The eating type of viscera was also investigated by using questionnaires. When ELISA method was used, the percentage of Toxoplasma infect swine among the conventionally raised and of those raised in swine specialized farms were 60.7 % and 21.3 %, respectively. The respective antibody values (+- SD) were 0.589 (+- 0.310) and 0.385 (+- 0.237) and differed very significantly (p<0.01). A significant difference was also found in antibody values among 6 villages (p<0.05). The mean infection percentage of Toxoplasma in the cat was 38.2 %, the infection percentage for cats raised under free-moving and restrained condition were 37.0 % and 38.2 % respectively. The respective antibody values (+- SD) for Toxoplasma were 0.600 (+- 0.614) and 0.637 (0.645), and did not differ significantly. The infection percentage of Toxoplasma in villagers and butchers were 26.2 and 38.3 % respectively. The respective antibody values (SD) for toxoplasma were 0.429 (+- 0.195) and 0.341 (+- 0.236), and differed very significantly (p<0.01). There were also highly significant differences Pyo-sun and other village (p<0.01). Analysis of the questionnaires showed that 26.0 % of 392 villages ate liver and some villagers ate other viscera.

Demi-embryos were successfully produced by bisection of ICR mouse embryos at preimplantation stages. They were microsurgically bisected using a microsurgical blade attached to a micromanipulator after pretreatment with 0.5 % pronase in PBS for two minutes or not. Embryos with softened zona pellucida were more easily bisected and less damaged than intact embryos. The highest success rate in bisection has been achieved by selecting blastocysts (94.1 % in success rate with intact blastocysts and 100 % in success rate with zona softened blastocysts). Demi-embryos without zona pellucida were cultured in D-PBS or M-16 medium at 37deg C, 5 % CO2 in air for 72 hours for 2-cell stage embryos, 48 hours for 4-to 8-cell stage embryos, 24 hours for morula stage embryos and 6-12 hours for blastocyst stage embryos. For the in vitro culture of 2-cell stage embryos, 100 micro M 2Na-EDTA was added to the media. M-16 medium was better for the in vitro development of mouse embryos than PBS, and PBS is not considered to be suitable for long-term culture of embryos, especially at early stage of cleavage. In M-16 medium, developing rate of demi-embryos of which pair underwent development to form eublastocysts was 15.8 % at 2-cell stage, 16.8 % at 4-cell stage, 38 % at 8-cell stage, 89.6 % at morula stage and 94.4 % at blastocyst stage, respectively. The more rapid and efficient production of demi-embryos and higher viability after bisection can be expected by softening zona pellucida with pronase and by selecting morulae or blastocysts rather than embryos at early stage of cleavage.