Johne's disease is one of the main causes of economic losses in ruminants and a major health hazard in the developed and developing developed countries.In this study, PCR detection of insertion sequences IS900 of MAP in the buffy coat of cows (n = 81), of this 29 (35.8%) cow showed positive results. By Restriction fragment length polymorphism (PCR-RFLP),two single nucleotide polymorphisms (SNPs) of SLC11A1 gene were tested for finding their association with susceptibility to bovine Johens disease in Iraqi cattle. A total of 50 cows were tested, theirresult revealedthat at rs109453173 locus twoelectromorph‘CC’ (374 bp) and ‘CG’ (374, 293 and 81 bp).The rs109915208 locus also showed two electromorph, ‘TT (344bp) and ‘CT’ (344, 215 and 129 bp) . The differences in the electromorph between IS900 positive and negative cows were found to be statistically significant (p = 0.0031). No significant difference in these electromorph at SNP locus rs109915208 between IS900 positive and negative cows. Out of two SNPs from SLC11A1 gene, rs109453173 had a significant association with the susceptibility to Johne's disease. The CC’ electromorph observed at rs109453173 locus showed a significant association with the susceptibility to bovine paratuberculosis in cows. The OR of ‘CC’  in‘IS900  positive versusIS900 negative cattle was7.8750, suggesting that cows having ‘CC’ electromorph were susceptible to Johne's disease compared to ‘CG’ electromorph .  

The objective of the current study was to evaluate the healing of experimentally severed Achilles tendons of the dogs repaired by Kessler suture pattern alone or with autologous peritoneal scaffold. A total of twelve healthy, neurologically and orthopedically local breed dogs were used in this study, which were randomly allocated into two equal groups (6 of each). The 1st group as a control, while the 2nd as a treated group. In both groups the Achilles tendon of one hind limb was completely severed transversely, in 1st group the tendon was repaired by Kessler suture pattern using silk No.3, while in the 2nd group a combination of Kessler suture and autogenous peritoneal scaffold was applied to reinforce the tendon repair. The site of surgery was evaluated clinically, while gross and histological study was performed at 15, 30 and 60 days post-operative days. Results was revealed that autologous peritoneum graft has a remarkable effects on Achilles tendon healing represented by improving fibroblastic proliferation, reduce adhesion, increase tendon strength and improve tendon function when compared with the control group. We concluded that peritoneal graft application has valuable and beneficial effects on treatment of ruptured Achilles tendon in dogs when utilizing it in conjunction with the traditional procedures.

Rabies cases are widespread worldwide and mostly prevalence in develeloping countries. Several Province in Indonesia are still categorized as endemic rabies, including the Province of North Sumatera. This research was aimed to evaluate the role of external factors that could influence in reduction the rabies suspected cases in the population of the City of Medan. The survey was conducted using a structured questionnaire. A Case-Control Design was used in this study. The two groups were rabies suspected case and rabies unsuspected case (control), consisted both male and female respondents. All the population involved in this study were living in the City of Medan. Primary and secondary data were collected usingpurposive samplingapproach. Data were analysed by univariate analysis, bivariate analysis, and multivariate analysis. The result was obtained the ratio of 1:2 between rabies suspected case (64 cases) and rabies unsuspected case (128 cases) in the City of Medan. The bivariate analysis showed the significant influence of the role of livestock department officer, role of health worker, role of community leader and vaccination tools on the rabies suspected case (P0.05). Multivariate analysis showed that the dominant variable which affect the rabis suspected case was rabies vaccine tools.

Interprofessional Education (IPE)is considered an educational strategy that has potential benefit forimproving teamwork and collaboration in practice, leading to improved patient outcomes. As the development and implementation of curricula that integrate IPE requires significant resources, its adoption should be based on evidence of effectiveness. The purpose of this literature review is to identify what constitutes effective interprofessional education amongst health related disciplines. A literature search was conducted using Science Direct, BMC, Elsevier and Joint Commission Journal for the years 2009-2018. A total of 15 studies met the established inclusion criteria. The use of IPE among health related disciplines is one effective way to solve several problems, and its particularly improves patient safety. What constitutes effective interprofessional education amongst health related disciplines are possibly viewed from some aspects, such as efficiency, effectiveness, learning strategy, power, patient and learner safety and readiness to practice, interprofessional curriculum, roles and relationships, and learning outcomes.

The presence of lactic acid bacteria (LAB) and histamine in foodstuffs indicate the level of deterioration in the quality of food and cause poisoning.Sie Baluis the Acehnese dried meat preserved by the addition of salt, acid and dried, but the long processing and drying it under the sun can cause microbial contamination in meat products. Irradiation can eliminate bacteria in foodstuffs. This study aimed to determine the amount of LAB and histamine levels ofSie Baluafter irradiation doses of 5, 7 and 9 kGy and stored 2 to 4 months.Sie Baluwas made of fresh beef 5 kg, dried in the sun to dry, vacuumed and irradiated with gamma rays. The samples for LAB determination cultured in MRS agar and incubated at 37C for 24 hours. The number of colonies was counted using Total Plate Count. The histamine level ofSie Baluconducted by ELISA. Irradiation did not significantly (P0.05) affect the amount of LAB, but the shelf life significantly (P0.05) affected the amount of LAB inSie Balu. Extending the shelf life up to 4 months can increase the amount of LAB. Irradiation dose and shelf life had no effect on histamine levels ofSie Balu(P0.05). This study concluded that irradiatedSie Balucannot be stored for more than two months.

This study aimed to study the effect of antibiotic on phospholipase production ofStaphylococcus aureusisolated from preputium of Aceh cattle. The parameters measured in this study were PzI ofStaphylococcus aureuswithout any treatment, and PzI ofS. aureusafter being given antibiotics. The experiment was carried out by modifying the Samaranayake method on egg yolk agar media, incubated at 37 C for 48 hours, and the precipitation lines formed was measured. The antibiotics used were tetracycline, oxytetracycline and fosfomycin with concentrations of 10%, 20% and 30% (mg / mL, respectively). The results showed that phospholipase production ofS. aureusisolated from preputium of Aceh cattle was suppressed, antibiotics were able to inhibit phospholipase production. However, the enzyme was still produced in positive category, with PzI = 0.287, the highest result was found in 30% tetracycline activity, which is equal to PzI = 0.341 and the lowest was in 10% fosfomycin which is equal to PzI = 0.332

The research was conducted in Sumber Harapan Village, Tinggi Raja District, Asahan Regency, North Sumatra Province, in 2016, using field survey methods, questionnaires and interviews. Secondary data was obtained from the local Livestock Service Office, primary data sourced from respondents by purposive random sampling, as many as 15 of goat farmer in scale of 3-5 heads / farmers and 10 cocoa farmers. Each farmer has a land area of around 1000 m2 and has 100 cocoa trees, producing around 5.7 to 6.5 kg/tree/ week. Data were analyzed descriptively, quantitatively and economic feasibility analysis of B/C ratio. The purpose of this study was to analyze the economic feasibility of goat and cocoa farming businesses. The results showed that, almost all of the land belonged by farmers. The farmer business continue to run well, the worker of the goats business and cocoa is quite different. For the goats business as much as 160.56 days/year and for cocoa businesses 262.28 days/year. The profit of goat as much 5.392.060 IDR/year B/C 1.8. The capital of expenditure was 1.714.200 IDR/year. So that the profits obtained 3.677.860 IDR/year. The advantage of cocoa farmers is 67.507.200 IDR/ year B/C 2.7, The capital of expenditure of 7.2142.00 IDR/year. So that the cocoa profits obtained of 60.293.000 IDR/year. It is concluded that the goat and cacao business can be profitable for farmers. Then the business as financially economically feasible to develop further.

In poultry, feeding diets including high concentrations of non-starch polysaccharides limits growth rate and feed conversion ratio, and causes problems in health and welfare because of the production of wet droppings. This problem is becoming more important as pressure builds to reduce costs by using alternative sources of dietary protein, such as lupin grain, rather than fish- or soybean-meal. We therefore tested whether enzymes that break down non-starch polysaccharides can overcome the problems with a lupin-based diet fed to Japanese quail. Chicks (18 days old) were allocated among 8 treatments, each replicated 3 times, with 12 chicks per replicate (ie, 36 birds per treatment). Chicks were fed diets formulated to contain 24% crude protein and 12 MJ/kg apparent metabolisable energy (AME). The diets included 10% or 20% lupin meal and, for each level of lupin, they were formulated in one of four ways: 1) no enzyme; 2) pectinase (1.4 U/g polygalacturonase and 0.2 U/g pectinesterase); 3) xylanase (1,4 endo-xylanase; 0.38 U/g); 4) combination of the above pectinase and xylanase treatments. The results indicated that, from age 28 days of age, both lupin content and enzyme treatment significantly (P 0.05) affected chick performance. Compared with the no-enzyme control, enzyme treatments improved growth at 35 days by 45-50% (P 0.05) and feed conversion ratio by 20-30% (P 0.05) for both levels of lupin content. In addition, dry matter digestibility and apparent metabolizable energy were significantly improved by the combined enzyme treatment for both levels of lupin inclusion. We conclude that pectinase and xylanase can overcome the negative effects of the non-starch polysaccharides in lupin meal, improving the growth of quail chicks fed lupin-based diets, and that the enzymes work best when combined.

The success breeding business of PE goat is closely related to reproductive performance and mortality rates between maternal and calve so that a case study is needed on the reproductive performance of PE Doe. The study was conducted in Taruna Sejahtera Group in Simalungun District, North Sumatera. Twenty nine of PE Does was used. Method was case study. Data was taken from recording list and interview then anlyzed descriptively. The results showed that reproduction characteristics of PE Doe: puberty age 10 month, first mating age 14 month, litter sizes 2 heads, weaning age 2 month, days open 90 days (3 month), post partum mating 4-7 month, calving interval 10 month, milk production averaged 1,2 liter/head/day and parity 4-5 times/head.

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum -lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective