خيارات البحث
النتائج 551 - 560 من 580
Predictive risk model of livestock rabies occurrence in Peru النص الكامل
2021
Ricardo Augusto Dias | Francisco Miroslav Ulloa-Stanojlovic
Livestock rabies is endemic in Peru. Hence, its persistence and annual dissemination represent an important economic impact, especially for impoverished farming communities. The disease is mostly transmitted by the hematophagous bat Desmodus rotundus. The present study aimed to adapt an existing predictive model of the occurrence of livestock rabies to Peru, in which the risk of rabies transmission from bats to livestock was estimated using decision-tree models of receptivity and vulnerability. Official rabies surveillance data between 2010 and 2015 were used along with possible risk factors, such as livestock biomass, environmental changes, and geomorphological characteristics. Several scenarios were established to evaluate the prediction of the occurrence of livestock rabies cases by determining more than one cut-off point of the receptivity variables. During the study period, the precision of the model was estimated through the sensitivity (39.46%) and specificity (98.64%) by using confusion matrices. Targeting control efforts, especially in districts with a high estimated risk, could represent the prevention of a significant proportion of livestock rabies cases, which would optimize the human and economic resources of the Peruvian surveillance service. However, the quality of data produced by the surveillance should be improved not only to obtain higher model precision but also to allow the adequate planning of control actions.
اظهر المزيد [+] اقل [-]Erratum: Investigation of Feline calicivirus infection in cats with upper respiratory tract disease in Diyarbakir, Turkey النص الكامل
2021
Nazan Baksi | Aynur Simsek
Feline calicivirus is among the most common pathogenic microorganisms in upper respiratory tract disease (URTD) and oral lesions of cats. It leads to stomatitis, oral ulceration, ocular and nasal discharge, conjunctivitis, fever, lameness, anorexia, hypersalivation, pneumonia, respiratory distress, coughing, and depression in infected cats. This study aimed to determine the role of Feline calicivirus (FCV) in cats with the upper respiratory tract disease in the Diyarbakir region, Turkey, to provide treatment for infected cats and contribute to the disease prophylaxis. The study material consisted of 10 cats (control group) considered to be healthy according to the clinical examination and 20 cats with URTD that were not vaccinated against Feline calicivirus infection of different breeds, ages, and genders brought to Dicle University Veterinary Faculty Prof. Dr. Servet SEKIN Polyclinic with URTD. After routine clinical examinations of the animals, oral and conjunctival swabs and blood samples were taken. Hematological and biochemical analyzes of blood samples were performed. Swab samples were analyzed by the polymerase chain reaction (PCR) method for the diagnosis of the agent. Oral lesions, hypersalivation, ocular and nasal discharge, coughing, and breathing difficulties were seen in clinical examinations of cats with URTD. Feline calicivirus was detected in only one cat's conjunctival swab sample in PCR analyses. As a result, we found that Feline calicivirus infection was present in cats with URTD in the Diyarbakir region, and 5% positivity was found in cats with clinical symptoms according to PCR analysis.
اظهر المزيد [+] اقل [-]Allele and genotype frequency for milk beta-casein in dairy cattle in the northern region of Tocantins State, Brazil النص الكامل
2021
Rodolfo Olinto Rotoli Garcia Oliveira | Minos Esperandio de Carvalho | Matheus Henrique Dias Rodrigues | Mirele Daiana Poleti | José Bento Sterman Ferraz | Ana Beatriz Bezerra Souza | Silvia Minharro | Helcileia Dias Santos | Jorge Luís Ferreira
At present, there is a concern about the quality of milk and diseases related to its consumption, as it can generate discomfort and allergic reactions in some individuals due to its protein components. Thus, the present study was developed to identify the allele and genotype frequencies of genes for β casein, A1 and A2, in dairy herds in the region of Araguaína-TO, Brazil. Genetic material from 421 animals (crossbred dairy cattle in lactation) was used. All animals were numbered for identification, and DNA samples were extracted from hair bulbs. Samples for two markers from the polymorphic regions were characterized and confirmed by real time PCR using the ABI Prism® 7500 Sequence Detection System (Applied Biosystems). Allele and genotype frequencies were determined using the TaqMan™ detection system, where the primer and probe release different fluorescence signals for each allele of the polymorphism. The sampled herd showed frequencies of 28.27% for the A1 allele and 71.73% for the A2 allele. Genotype frequencies were 52.96% (223/421) for A2A2; 37.53% (158/421) for the A1A2 genotype; and 9.50% (40/421) for the A1A1 genotype. The frequency of the A1 allele for β-casein in dairy herds from the northern region of Tocantins was low and is per the results of previous studies. Although the A2A2 genotype of β-casein had a high relative frequency, the A1A2 genotype is still rather frequent, warranting greater selection pressure.
اظهر المزيد [+] اقل [-]The reality of the donkey’s exploitation for the hide trade in Brazil: disease outbreaks and animal welfare compromised in rescued donkeys النص الكامل
2021
Lucas Santana da Fonseca | Aline Rocha Silva | Frederico Augusto Mazzocca Lopes Rodrigues | Sidnei Miyoshi Sakamoto | Vania de Fátima Plaza Nunes | Adroaldo José Zanella | Chiara Albano de Araujo Oliveira | Pierre Barnabé Escodro
About 800 donkeys that were confined in a restrictive area used in a manner comparable to a warehouse for receiving donkeys for slaughter were abandoned. After receiving reports of mistreatment, civilians acted to save the animals. A task force was organized that planned veterinary and zootechnical actions and activities for daily health management, feeding, and clinical care to attend to the abandoned donkeys. Positive cases were diagnosed for glanders, equine infectious anemia, equine herpesvirus, and equine babesiosis. The objective of this communication is to bring to the attention of the scientific community the interventions in the area of animal health and welfare, to address the episode of northeast donkeys that were victims of international trade. It is fundamental to change the approach related to the management of donkeys in Brazil, and appeal to the necessity to identify ethical and sustainable ways to incorporate donkeys in Brazil in the 21st century.
اظهر المزيد [+] اقل [-]Characteristics of Acute Otitis Media Patients at The Citra Medan Perjuangan Clinic in 2020 النص الكامل
2021
Annisa Lismarani | Dewi Ratnasari | Azwar Ridwan
Otitis media is inflammation of part or all of the middle ear mucosa, Eustachian tube, mastoid antrum, and mastoid cells. Otitis media is less common in adults than in children and is more common in men. The clinical symptoms of AOM depend on the stage of the disease and the age of the patient. The purpose of this study was to determine the characteristics of patients with acute otitis media in the field of battle image clinic in 2020 based on gender, age, and clinical symptoms. This research was conducted by collecting data from medical records at the citra medan perjuangan clinic in 2020. Then the data was processed using a computer, and the results were analyzed descriptively by univariate analysis. The number of samples that have been collected is 72 people. The highest distribution was male sex at 61.1%, age 1-5 years at 61.1%, and fever at 58.3%. Further research is needed to determine the risk factors for AOM that can be used as prevention.
اظهر المزيد [+] اقل [-]Combination of Isoniazid and Rifampin for the Prevention of Failure of Standard Therapy for Latent Tuberculosis Infection in Children: Evidence Base Case Report النص الكامل
2021
Bakhtiar Bakhtiar | Hirsa Angriani
Tuberculosis (TB) control program in children is carried out with three strategies: finding and treating adult TB cases (as a source of transmission), treating latent TB infection, and administering the BCG vaccine. There is a high risk of transmission in children in close contact and living at home with adult patients. After Mycobacterium is inhaled through the respiratory tract, then the body's resistance to Mycobacterium tuberculosis occurs. Furthermore, there are two possibilities: only TB infection occurs, or the child has TB disease. We report one case: a boy, age four years, diagnosed with latent TB infection. This diagnosis is based on the findings of the 13 mm tuberculin test (positive). There is a history of contact with adult TB patients. On physical examination, no abnormalities were found. Likewise, the chest X-ray examination also found no abnormalities. Due to the diagnosis of latent TB infection, prophylaxis with Isoniazid was planned for six months. In the first month, the child still wants to take medication according to the rules. However, entering the second month, children sometimes do not want to take medication. This situation raises concerns about the possibility of therapy failure. His mother was very worried about this condition. Therefore, doctors offer to provide a combination pattern of therapy in isoniazid with rifampicin, with a span of 3 months. Standard therapy with INH alone was discontinued. This therapeutic decision was based on a study that combined INH with rifampin for three months gave better results than single prophylactic therapy with INH.
اظهر المزيد [+] اقل [-]Heifer management characterization in dairy herds from the west of Buenos Aires, Argentina النص الكامل
2021
Federico Demateis Llera | Claudina Vissio | María Paula Turiello | María Alejandra Herrero | Alejandro José Larriestra
Heifer management characterization in dairy herds from the west of Buenos Aires, Argentina النص الكامل
2021
Federico Demateis Llera | Claudina Vissio | María Paula Turiello | María Alejandra Herrero | Alejandro José Larriestra
The replacement program in a dairy farm represents the second or third largest cost in a dairy operation. This study aimed to characterize and typify the practices related to the dairy heifer replacement program and describe the growth, development, and health parameters during this period in commercial dairy herds in Trenque Lauquen. A cross-sectional observational study was carried out, including 54 randomly selected dairy farms that were visited once to collect data about facilities and management through a semi-structured survey. Cluster and principal coordinates analysis were applied to classify the farms based on all variables collected, grouped in four main areas: pre-fresh cows, colostrum management, pre-weaning calf ’s management, and personnel. Additionally, growth, development, and health status were also recorded and described for calves and breeding heifers. Two similar-sized farm clusters were identified with differences in management and facilities in different areas of calves rearing. In one cluster there was a greater proportion of farms having a pre-fresh group, implementing appropriate health (colostrum, vaccination) and feeding management. Also, differences in personnel and technical support were relevant. The estimated body gain was 452 and 774 g/d for calves younger or older than 60 d of age, respectively. The age and weight farm averages in breeding heifers were 21.0 mo (range: 16.7-27.5) and 416.3 kg (range: 336.7-519.3), respectively. Diarrhea and respiratory affections were the major problems in pre-weaning calves and heifer rearing, respectively. The median mortality was 7.3, 7.6, and 2.9% at the calving, pre-weaning, and heifer rearing period, respectively. The results showed an improvement opportunity for producers, the design of precise and high impact programs that could lead to an improved replacement program.
اظهر المزيد [+] اقل [-]Heifer management characterization in dairy herds from the west of Buenos Aires, Argentina | Caracterização da gestão de novilhas em rebanhos leiteiros do oeste de Buenos Aires, Argentina النص الكامل
2021
Demateis Lllera, Federico | Vissio, Claudina | Turiello, María Paula | Herrero, Alejandra | Larriestra, Alejandro Jose
The replacement program in a dairy farm represents the second or third largest cost in a dairy operation. This study aimed to characterize and typify the practices related to the dairy heifer replacement program and describe the growth, development, and health parameters during this period in commercial dairy herds in Trenque Lauquen. A cross-sectional observational study was carried out, including 54 randomly selected dairy farms that were visited once to collect data about facilities and management through a semi-structured survey. Cluster and principal coordinates analysis were applied to classify the farms based on all variables collected, grouped in four main areas: pre-fresh cows, colostrum management, pre-weaning calf ’s management, and personnel. Additionally, growth, development, and health status were also recorded and described for calves and breeding heifers. Two similar-sized farm clusters were identified with differences in management and facilities in different areas of calves rearing. In one cluster there was a greater proportion of farms having a pre-fresh group, implementing appropriate health (colostrum, vaccination) and feeding management. Also, differences in personnel and technical support were relevant. The estimated body gain was 452 and 774 g/d for calves younger or older than 60 d of age, respectively. The age and weight farm averages in breeding heifers were 21.0 mo (range: 16.7-27.5) and 416.3 kg (range: 336.7-519.3), respectively. Diarrhea and respiratory affections were the major problems in pre-weaning calves and heifer rearing, respectively. The median mortality was 7.3, 7.6, and 2.9% at the calving, pre-weaning, and heifer rearing period, respectively. The results showed an improvement opportunity for producers, the design of precise and high impact programs that could lead to an improved replacement program. | Nos rebanhos leiteiros, o programa de reposição representa o segundo ou o terceiro maior custo da atividade de produção de leite. O objetivo do presente trabalho foi caracterizar e tipificar as práticas relacionadas ao programa de reposição de novilhas leiteiras e descrever o crescimento, desenvolvimento e parâmetros de saúde durante este período em rebanhos leiteiros comerciais localizados em Trenque Lauquen, oeste de Buenos Aires, Argentina. Foi realizado um estudo observacional transversal incluindo 54 fazendas de produção de leite selecionadas ao acaso e visitadas uma única vez para a colheita de dados relacionados a instalações e manejo empregando-se um questionário semi-estruturado. Foi efetuada a análise dos agrupamentos e das coordenadas principais para classificar as fazendas com base em todas as variáveis colhidas e agrupadas em quatro principais áreas: vacas pré-parto, manejo de colostro, manejo pré-desmame de bezerros e recursos humanos. Os dados referentes ao crescimento, desenvolvimento e estado de saúde dos animais também foram registrados e descritos para bezerros e novilhas. Dois grupos de fazendas de tamanho semelhante foram identificados com diferenças no manejo e instalações em distintas áreas de recria de bezerros. Em um grupo houve uma maior proporção de fazendas que tinham um grupo de vacas pré-parto, com implementação de adequada manejo de saúde (colostro, vacinações) e manejo alimentar, também, foram relevantes as diferenças em termos de pessoal e suporte técnico. O ganho de peso estimado foi de 452 e 774 gramas por dia, respectivamente, para os bezerros jovens e os com idade superior a 60 dias. As médias das fazendas da idade e do peso das novilhas de reprodução foram, respectivamente, 21,0 meses (variação de 16,7 a 27,5) e 416,3 kg (variação de 336,7 a 519,3). A diarreia e as afecções respiratórias foram os maiores problemas de saúde, respectivamente, nos bezerros em pré-desmama e novilhas em recria. A média de mortalidade foi de 7,3; 7,6 e 2,9%, respectivamente, ao parto, na pré-desmama e durante a recria de novilhas. Os resultados obtidos demonstraram a existência de oportunidades de melhoria para os produtores, com o delineamento de programas precisos e de alto impacto que poderão propiciar um programa de reposição aprimorado. | Fil: Demateis Lllera, Federico. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Norte. Estacion Experimental Agropecuaria General Villegas. Agencia de Extension Rural Trenque Lauquen.; Argentina | Fil: Vissio, Claudina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina | Fil: Turiello, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Producción Animal; Argentina | Fil: Herrero, Alejandra. Universidad de Buenos Aires; Argentina | Fil: Larriestra, Alejandro Jose. Universidad Nacional de Villa María. Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina
اظهر المزيد [+] اقل [-]In vitro impact of condensed tannins on the caecal metabolites of chickens النص الكامل
2021
Mohammed Munis Dakheel | Mohammed Abdalmalek Ali Al-Bedhawi | Afnan Ahmed Al-Mnaser | Martin John Woodward | Marina Mora-Ortiz | Caroline Rymer
In vitro impact of condensed tannins on the caecal metabolites of chickens النص الكامل
2021
Mohammed Munis Dakheel | Mohammed Abdalmalek Ali Al-Bedhawi | Afnan Ahmed Al-Mnaser | Martin John Woodward | Marina Mora-Ortiz | Caroline Rymer
Tannins are a diverse group of plant phenolic compounds. Condensed tannins (CTs) represent a major subgroup of tannins and were extracted from tilia (Tilia L.) flowers and black locust (Robinia pseudoacacia) leaves. These extracts were examined for their effects on the metabolic profile of chicken caeca. By using in vitro, a nuclear magnetic resonance (1H-NMR), which was combined with multivariate statistics, the current study was applied for the first time to investigate how three different CT compositions, procyanidins (PC) and/or prodelphinidins (PD) units influenced the metabolic end-products in caecal contents of chickens. In the presence of tannins, glutamate, leucine, lysine, pyroglutamate, phenylalanine, proline, and sarcosine were significantly decreased. CT extracts significantly influenced the fermentation, increasing the concentrations of some fatty acids such as acetate, butyrate, and propionate whereas. In contrast, lactate decreased between the treatments. This study identified the key structural features of CTs that contain either high molar proportions of PD or PC, which might be useful to improve the efficiency of feed utilization in chickens.
اظهر المزيد [+] اقل [-]In vitro impact of condensed tannins on the caecal metabolites of chickens النص الكامل
2021
Dakheel, Mohammed Munis | Al-Bedhawi, Mohammed Abdalmalek Ali | Al-Mnaser, Afnan Ahmed | Woodward, Martin John | Mora-Ortiz, Marina | Rymer, Caroline
Tannins are a diverse group of plant phenolic compounds. Condensed tannins (CTs) represent a major subgroup of tannins and were extracted from tilia (Tilia L.) flowers and black locust (Robinia pseudoacacia) leaves. These extracts were examined for their effects on the metabolic profile of chicken caeca. By using in vitro, a nuclear magnetic resonance (1H-NMR), which was combined with multivariate statistics, the current study was applied for the first time to investigate how three different CT compositions, procyanidins (PC) and/or prodelphinidins (PD) units influenced the metabolic end-products in caecal contents of chickens. In the presence of tannins, glutamate, leucine, lysine, pyroglutamate, phenylalanine, proline, and sarcosine were significantly decreased. CT extracts significantly influenced the fermentation, increasing the concentrations of some fatty acids such as acetate, butyrate, and propionate whereas. In contrast, lactate decreased between the treatments. This study identified the key structural features of CTs that contain either high molar proportions of PD or PC, which might be useful to improve the efficiency of feed utilization in chickens.
اظهر المزيد [+] اقل [-]De acordo com o governador, a decisão vale para todos que estejam em condições físicas adequadas, mesmo os que ainda não tomaram a vacina contra a Covid-19 النص الكامل
2021
Yudit Rodríguez Coipel | Aníbal Domínguez Odio | Odaimis Mena Álvarez | Raiselys Toirac Proenza | Isbel González Marrero | Daniel Leonardo Cala Delgado
De acordo com o governador, a decisão vale para todos que estejam em condições físicas adequadas, mesmo os que ainda não tomaram a vacina contra a Covid-19 النص الكامل
2021
Yudit Rodríguez Coipel | Aníbal Domínguez Odio | Odaimis Mena Álvarez | Raiselys Toirac Proenza | Isbel González Marrero | Daniel Leonardo Cala Delgado
The industrial manufacturing of natural products for veterinary use represents a major weakness in the veterinary sector despite increased interest and the traditional ancestral knowledge that supports them. A cross-sectional, descriptive observational study was conducted to characterize the veterinary herbal products marketed worldwide during 2018 and 2019. A comprehensive thematic search limited to the 2018-2019 period was performed in the ScienceDirect, Scopus, PubMed, Web of Science, ResearchGate, and Academic Search Complete databases. The investigation identified 487 products registered in the global market manufactured by 54 companies, led by India, The Netherlands, and the United Kingdom. The market segments of animal production and phytomedicines were dominant with 73.7% and 53.0% of products, respectively. Cattle (22.2%), sheep-goats (16.2%), and canines (16.2%) were the most favored species. The most represented therapeutic indications were those intended to treat gastrointestinal disorders (30.47%), antimicrobials (16.66%), and antiparasitic agents (10.47%). The families Fabaceae, Lamiaceae, Asteraceae, Apiaceae, Malvaceae, and Rutaceae stood out because of their frequent use, encompassing 35.0% of the 137 declared species. Andrographis paniculata (Burm.f.) Nees and Withania somnifera (Lin) Dunal were the most important species. Oral formulations for internal use (72%) and liquids (51%) in 100 mL, 500 mL, and 1 L presentations showed the highest prevalence on the market. The global market for veterinary herbal products during the 2018-2019 period was relevant in the productive and medical animal sector. No differences were found between medicinal plant species used to formulate herbal products for human and animal use.
اظهر المزيد [+] اقل [-]Global market for veterinary herbal products during the 2018-2019 period النص الكامل
2021
Cala Delgado, Daniel Leonardo | Domínguez Odio, Aníbal | Mena Alvares, Odaimis | Toirac Proenza, Raiselys | González Marrero, Isbel
La fabricación industrial de productos naturales para uso animal es una de las grandes debilidades del sector veterinario, a pesar de existir un creciente interés por ellos y ancestrales saberes tradicionales que lo respaldan. Sobre esta problemática, se realizó un estudio observacional descriptivo transversal para caracterizar los productos herbales veterinarios comercializados a nivel mundial en el periodo 2018-2019. Se efectuó una búsqueda temática exhaustiva en bases de datos (ScienceDirect, Scopus, PubMed y Web of Science, ResearchGate y Academic Search Complete), limitada al periodo 2018-2019. El estudio encontró 487 productos registrados en el mercado mundial, fabricados por 54 compañías, lideradas por la India, Holanda y Reino Unido. Los segmentos: animal productivo y fitomedicamento dominan el mercado con el 73.7% y 53.0% de los productos respectivamente. Los bovinos (22.2%), ovino-caprinos (16.2%); y caninos (16.2%) fueron las especies más favorecidas. Las indicaciones terapéuticas más representadas son los destinadas a corregir trastornos gastrointestinales (30.47%), antimicrobianos (16.66%) y antiparasitarios (10.47%). Por sus altos niveles de uso sobresalen las familias Fabaceae, Lamiaceae, Asteraceae, Apiaceae, Malvaceae y Rutaceae quienes abarcan el 35.0% de las 137 especies declaradas y de ellas las especies Andrographis paniculata (Burm.f.) Nees y Withania somnífera (L.) Dunal, son las de mayor importancia. Se demostró que las formulaciones de uso interno por vía oral (72%) y las líquidas (51%) en envases por 100 y 500 mL y 1L son las que prevalecen en el mercado. | The industrial manufacturing of natural products for veterinary use represents a major weakness in the veterinary sector despite increased interest and the traditional ancestral knowledge that supports them. A cross-sectional, descriptive observational study was conducted to characterize the veterinary herbal products marketed worldwide during 2018 and 2019. A comprehensive thematic search limited to the 2018-2019 period was performed in the ScienceDirect, Scopus, PubMed, Web of Science, ResearchGate, and Academic Search Complete databases. The investigation identified 487 products registered in the global market manufactured by 54 companies, led by India, The Netherlands, and the United Kingdom. The market segments of animal production and phytomedicines were dominant with 73.7% and 53.0% of products, respectively. Cattle (22.2%), sheep-goats (16.2%), and canines (16.2%) were the most favored species. The most represented therapeutic indications were those intended to treat gastrointestinal disorders (30.47%), antimicrobials (16.66%), and antiparasitic agents (10.47%). The families Fabaceae, Lamiaceae, Asteraceae, Apiaceae, Malvaceae, and Rutaceae stood out because of their frequent use, encompassing 35.0% of the 137 declared species. Andrographis paniculata (Burm.f.) Nees and Withania somnifera (Lin) Dunal were the most important species. Oral formulations for internal use (72%) and liquids (51%) in 100 mL, 500 mL, and 1 L presentations showed the highest prevalence on the market. The global market for veterinary herbal products during the 2018-2019 period was relevant in the productive and medical animal sector. No differences were found between medicinal plant species used to formulate herbal products for human and animal use. | https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000085088&lang=es | https://orcid.org/0000-0003-4639-5952 | https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000000695 | daniel.cala@campusucc.edu.co | https://scholar.google.com.tw/citations?hl=es&pli=1&user=o7lD-k8AAAAJ
اظهر المزيد [+] اقل [-]Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen النص الكامل
2021
Carolina Natalia Alonso | Catalina Castañeira | Ana Flores Bragulat | Luis Losinno
Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed to evaluate the effect on sperm kinetic parameters and membrane integrity in cooled donkey semen diluted with defined milk proteins extender with 1% or 2% of egg yolk and the removal of seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into four aliquots that were diluted in extender with 1% (EY1) or 2% (EY2) egg yolk. One sample from each group was centrifuged, seminal plasma was removed (CEY1, CEY2 groups, respectively), and the samples were then refrigerated at 5 °C for 24 h. Fresh and cooled semen samples were assessed for sperm motility, morphology, and plasma membrane integrity. Total motility, progressive motility, sperm kinetic parameters, or live sperm cells were not statistically different when semen was cooled with an extender supplemented with 1% or 2% of egg yolk. Seminal plasma removal does not affect total motility or sperm kinetic parameters. However, progressive motility decreased (P<0.05) when semen was extended with 2% of egg yolk and seminal plasma was removed. Membrane integrity was affected (P<0.05) in centrifuged samples. In conclusion, the obtained results suggest that there is no difference in sperm kinetics and membrane integrity when 1% or 2% of egg yolk was added to the Equiplus(R) extender. Also, the removal of seminal plasma by centrifugation did not have any beneficial effect on cooled donkey semen. Further studies are needed to relate these results with in vivo fertility tests with cooled donkey semen.
اظهر المزيد [+] اقل [-]