Activity of amylolytic, proteolytic, and lipolytic enzymes in the content and mucosa of intestinal tract, alkaline phosphatase in mucosa of intestinal tract, as well as the determination of calcium and phosphorous concentration in gastro-intestinal tract of hens were analyzed in the conditions of the Republic of Belarus. Results of analysis of the obtained experiment results of laying hens of parental stock by influence of various vitamin-mineral additives were presented. The highest activity of amylolytic, proteolytic, and lipolytic enzymes in 170-330 days old laying hens of Belarus-9 cross was marked in small intestine. There was stated the decreasing of enzymatic activity in proportion to removal from a stomach. At the same time, activity of alkaline phosphatase decreasds. Activity of alkaline phosphatase, proteolytic and starch-reducing enzymes in a mucous membrane of a duodenal gut was a bit higher than in mucosae of jejunal. Lipolytic enzymes showed high degree activity in mucosae of jejunal. Enzymic activity in large bowel was low. Addition of iodine-containing preparation Kajod into hen layer diets increased the activity of alkaline phosphatase, of amylolytic, proteolytic, and lipolytic enzymes in intestines. Enzymic activity of mucous intestines at hens of breed Plimutrok in process of removal from stomach decreased. In course of the period from 240 to 330-days old age the activity of studied enzymes in hen intestines decreased. Replacement of mineral additive Cockleshell by Pikumin did not render negative influences on changes in digestive enzyme activity. In 170-days old laying hens of Belarus-9 crossbreed the calcium content increased from the stomach to the small intestine, and then decreased in thick department. In the 200-days old hens the calcium concentration in a stomach increased in both groups and gradually decreased in the department of small intestine, but in rectum its quantity increased in both groups. In the period of intensive egg-laying (280 days) the general calcium content in thin department of intestines was less than in the stomach, later it increased in cecum and decreased in rectum. By the end of the experiment (330 days) the concentration of calcium in control group decreased from the stomach to the beginning of thick department of intestines, but in rectum its quantity increased. In experimental group the calcium content increased from a stomach to cecums and then decreased in rectum. In distribution of inorganic phosphorus in gastrointestinal tract departments at hens of various age groups the tendency to increase in its quantity in small intestine and the beginning of thick department of intestines, and then to reduction in rectum was observed. And at hens receiving premix Ajdeko, the phosphorus content, since 200-day age, was higher, than at hens receiving the basic ration

Results of studies of the influence of two biological products produced on the basis of sapropel (putrid mud) of the Ganarata lake (Mostovski district, the Republic of Belarus) on quality of legume-grass silage were presented. It was established, that analyzed preparations could be used as the preserving agents for preparation of herbal forages. The preserving action of the preparations was caused by the intensification of lactic bacteria development in silaging mass for the account of humic acids, as well as by the fungicidal action of phenolic and carboxylic fractions of the preparation. Silage preparation in combinations with putrid mud preparations made it possible to lower the losses of a crude protein on 11,3-15,6%, sugar - on 33,3%, carotin - on 20% and to obtain forage with concentration of exchange energy 9,20-9,38 mJ per 1 kg of dry matter. Silage application in combination with biological products rendered positive impact on milking efficiency of cows, the average daily milk yields in the experimental cow groups were on 1,3-1,4 kg or 6,8-7,3% higher. Application of higher quality feeds made it possible to lower the expenses of forages on 0,04 fodder units. Research results proved that the analysed preparations from sapropel could be used as an efficient conservants for silage preparations. They made it possible to raise forage quality at the minimum expenses

Determination of the efficiency of young fat stock feeding with wet rolled maize grain which was preserved by means of different preparations was realized in the conditions of the Republic of Belarus. The following preserving agents were applied: HV-2 (by- product of amino-formaldehyde resin); AIV 3 Plus (mixture of 62% formic acid and 24% ammonium formate; KEMIRA, Finland); carbamide (30 kg/t) with 40 g/t of complex mineral supplement. Feeding of young fat stock with rolled maize grains preserved by NV-2 promoted the increasing of nutrient digestibility on 1,5-5,0%, nitrogen use - on 2% in comparison with control. Application of rolled maize grain preserved with AIV-3Plus and carbamide with the complex mineral supplement made it possible to increase the digestibility of nutrients on 1,0-4,8% and nitrogen use - on 1,6-1,7%. Introduction of rolled maize grain preserved with NV-2 into rations of bull calves increased their productivity on 4,7% (P less than 0,05) with the simultaneous lowering of forage expenses on 1,9%. Application rolled maize grains preserved with AIV-3Plus made it possible to increase daily average weight gain on 3,8% and lower the forages expenses on 2,6%. Use preserved with NV-2 and AIV-3Plus rolled maize grains in rations of young fat stock lowered the production cost price on 11,9% and 10,8% that proved the efficiency of the studied fodder

Nowadays in connection with an interdiction for antibiotics use in animal feeding it is very actual to work out and apply new forms of probiotic preparations which should be as ecologically safe and harmless both to people and animal products. Probiotics make it possible to improve the digestibility of nutritive substances of forages, normalize metabolism, enrich with biologically active substances that promotes the increasing of their productivity. In the Republic of Belarus the probiotics are produced in the obviously insufficient quantity that creates problems of young stock livability. Influence of application of Akozil and Sublicin probiotics in diets of suckling calves on biochemical parameters of blood serum was analysed. It was stated that use of the analysed probiotics made it possible to decrease the level of urea and total cholesterol, to increase of the total protein content in blood serum of the experimental calves. There was noted the increasing of glucose level in blood serum of calves fed with Akozil. There was marked the tendency of more optimal level of calcium and phosphorus in calves having Sublicin in their diets. The obtained results testified the normalization of metabolic processes taking place in calves which were fed by the mentioned above probiotics

Improvement of the standards of energy and protein diets of young stock cattle of 1-6 month old and determination of its influence on animal productivity was realized in the conditions of the Republic of Belarus. The studied made it possible to determine that at the age of 1 month the calf diet should contain 1,02 kg of dry matter and 21,2 MJ of metabolic energy; at the age of 2 months - 1,26 kg and 23,5 MJ; at the age of 3 months - 2,2 kg and 25,6 MJ; at the age of 4 months - 2,9 kg and 31 MJ; at the age of 5 months - 3,28 kg and 34,2 MJ; at the age of 6 months - 3,76 kg and 37,6 MJ, respectively. Metabolic energy concentration per 1 kg of dry matter should not be lower than 20,7 MJ at the first month, 19 MJ - at the second month, after the third month it should lower up to 10 MJ. In course of 6 months the presented diet scheme made it possible get 832 g of average weight gain in the conditions of forage expenses of 3,52 forage units per 1 kg

In the Republic of Belarus the grain part of mixed fodders is basically formed by the following cereal cultures: wheat (Triticum aestivum), barley (Hordeum vulgare), oat (Avena saliva) and triticale. Rye is applied in the limited quantity in pig feeding despite all its advantages: large areas of cultivation, stable yielding capacity, low requirements to growing conditions, possibility to obtain ecologically clean production, low prime cost of grain. Rye in swine diets forms viscous solutions which break digestion process, worsen their fixation and accordingly reduce animal weight gain. Also, it was determined that rye grain had high quantity of pentosans (non-starch polysaccharides) which suppress the overall digestibility and accessibility of proteins. One of the methods of decreasing the negative influence of anti-nutritional properties of rye on pig organism and improving its digestibility is the application of fodder enzyme additives. In course of the realized investigation there was analyzed the influence of foreign fodder additives on rye-containing (Sk-21 – 10% and SK-26 – 20%) diets in swine rations. The following enzyme additives were analyzed: Rovabio TM Excel (France); Finase TM (Germany); Avizyme and Porzyme 8100 TM (United Kingdom). Application of enzyme additives Avizyme, Rovabio and Finase in mixed fodders containing rye (SK-21 - 10% of rye) for pigs at the age of 50-107 days made it possible to increase live weight on 16,8-21,7% and lower expenses for forages (fodder units) per 1kg of weight gain on - 12,3-18,2%. Intensity of live weight gain from 50-day up to 190-days age was the following: in a control group -80,8 kg, in the first experimental group fed with Rovabio - 88,6 kg, in the second experimental group fed with Avizyme - 89,7 kg, in the third experimental group fed with Finase - 84,6 kg. Research results showed that enzymes Avizyme and Rovabio rendered the highest influence on mixed fodders containing rye (SK-21 - 10% of rye and SK-26 - 20% of rye)

Research was directed towards selection of national, accessible and cheap ingredients of whole milk replacers in the conditions of the Republic of Belarus. The efficiency of compounding preparation and its use in calves feeding at an early age was analysed. There was developed the technological scheme of application of hydrodynamic installation which operates according cavitating principle and makes it possible to receive the homogeneous and finely divided mass directly from grain forage that is very important for preparation of milk replacer. New equipment gives the chance to lower power consumption on 17%, to receive economic benefit. During testing of developed of milk replacer-1 (used for calves feeding since one month old) and milk replacer-2 (used for calves feeding since two months old) and their comparison with the whole milk replacer Kalvolak (the Netherlands) it was revealed, that indices of a daily average weight gain in calves of the first group fed with foreign milk replacer, and in calves of the second groups, which were fed with the national milk replacer, did not show authentic differences (806 and 816 g). However, the prime cost of 1kg of live weight gain in the second case was on 43% cheaper than in the first one. The cost price of 1kg of live weight gain of calves on expenses for fodder units in trial group was on 33% cheaper in comparison with the control group

Analysis of supply of first-calf heifer diets with the mineral substances and their blood level at the first period of lactation as well as the development of the optimal feed formula of the mineral premix which could make it possible to neutralize the possible deficit of nutritive elements was realized in the conditions of the Republic of Belarus. It was stated that in animal diets against the background of protein and carbon misbalance there was noted the deficit of a number of trace elements such as manganese, copper, cobalt and zinc. In spite of sufficient content of calcium and phosphorus in cow diets, their concentration in blood plasma in first-calf heifers did not correspond to standard criteria, that was probably caused by vitamin D deficiency in cow rations. There was marked a low level of zinc, cobalt and manganese in whole blood of the majority of cows. With the purpose of correction of the first-calf heifer rations and for the prevention of possible hypomicroelementoses there was developed the composition of premix taking into account the necessary of cow feeding standards

The purpose of research is to study influence of fodder additive Water Trit liquid on dynamics of live weight, natural resistance and determination of an optimal dose for pigs on growing. Thanks to рН decrease of intestines contents and presence of organic acids the additive is applied for disease prevention. Presence of organic acids stimulates the development of useful Lactobacillus micro flora in intestines gleam. From the received data positive influence of the fodder additive on a gain of body weight is visible. In the beginning of experiment the difference in body weight of a control and a trial groups was insignificant and made from 9,2 kg to 9,4 kg in the experimental group, and 9,3 kg - in the control group. In the end of experiment the difference in body weight was essential: 28,6 kg, 30,5 kg, 29,5 kg in the trial groups and 28,05 kg in the control. After application of the fodder additive Water Trit liquid in the trial groups there were noted the raised daily average weight gains: on 2,6% in the second, on 13,4% (P less than 0,05) - in the third and on 8,5% - in the fourth group in relation to control group. For studying of the influence of the fodder additive on animal organism there was carried out the blood testing: phagocytosis, bactericidal activity of blood serum, lysozyme activity of blood serum. Indexes on phagocytal index at animal of the control and trial groups were in the beginning of experiment approximately at one level. At 45-day age the indexes in the second and fourth trial groups were above the control ones on 5,2% and 8,9%, respectively. By the end of the experiment the phagocytal index in all four groups changed insignificantly. At studying of lysozyme activity of blood serum at pigs the given research have shown, that at 30-day age the indexes in the third and fourth trial groups were above the control, and indexes in the first group were above the standard of the control. At 70-day age the highest lysozyme activity of blood serum was in the second trial group and has made +16,0% to the control. It is necessary to notice, that on the 70th day this indicator was above the control in all three trial groups

Comparative evaluation of the influence of cell-free probiotic preparation Laktimet and cellular probiotic Bifilak on productivity, livability, nonspecific response of suckling piglets in the early extrauterine life was realized in the conditions of the Republic of Belarus.Application of both probiotics increased the productivity of suckling pigs, stimulated the processes of metabolism and natural protection of organism in the early postnatal period in the conditions of specialized pig breeding complexes. Research results showed, that application of brand new cell-free probiotic Laktimet exercised higher influence on suckling pigs. Application of Laktimet made it possibler to increase piglets body weight gain on 7,8%; piglet livability - on 6,7%; and application cellular probiotic preparation Bifilak - on 5,4% and 3,3%, respectively. The same tendency was noted during the analysis of indexes of cellular factors of organism protection. Bactericidal activity of blood serum of experimental animals changed both in connection with age, and under influence of probiotic preparations. During all studied periods the bactericidal activity of blood serum was authentically (P less than 0,05) above in experimental groups in comparison with a control group. At 20-days old piglets there was stated the strengthening of bactericidal activity of blood serum. Throughout all period of experiment the superiority of trial animals over control on indicators of lysozyme activity of blood serum was observed