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النتائج 61 - 70 من 602
Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41, or 987P adhesins.
1992
Casey T.A. | Nagy B. | Moon H.W.
Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41, or 987P adhesins.
1992
Casey T.A. | Nagy B. | Moon H.W.
Three-week-old weaned and colostrum-deprived neonatal (< 1 day old) pigs were inoculated to determine the pathogenicity of 2 enterotoxigenic Escherichia coli isolates that do not express K88, K99, F41, or 987P adhesins (strains 2134 and 2171). Strains 2134 and 2171 were isolated from pigs that had diarrhea after weaning attributable to enterotoxigenic E coli infection. We found that both strains of E coli adhered in the ileum and caused diarrhea in pigs of both age groups. In control experiments, adherent bacteria were not seen in the ileum of pigs < 1 day old or 3 weeks old that were noninoculated or inoculated with a nonpathogenic strain of E coli. These control pigs did not develop diarrhea. Antisera raised against strains 2134 and 2171 and absorbed with the autologous strain, grown at 18 C, were used for bacterial-agglutination and colony-immunoblot assays. Both absorbed antisera reacted with strains 2134 and 2171, but not with strains that express K99, F41, or 987P adhesins. A cross-reaction was observed with 2 wild-type K88 strains, but not with a K12 strain that expresses K88 pili. Indirect immunofluorescence with these absorbed antisera revealed adherent bacteria in frozen sections of ileum from pigs infected with either strain. We concluded that these strains are pathogenic and express a common surface antigen that may be a novel adhesin in E coli strains that cause diarrhea in weaned pigs.
اظهر المزيد [+] اقل [-]Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41, or 987P adhesins
1992
Casey, T.A. | Nagy, B. | Moon, H.W.
Three-week-old weaned and colostrum-deprived neonatal (< 1 day old) pigs were inoculated to determine the pathogenicity of 2 enterotoxigenic Escherichia coli isolates that do not express K88, K99, F41, or 987P adhesins (strains 2134 and 2171). Strains 2134 and 2171 were isolated from pigs that had diarrhea after weaning attributable to enterotoxigenic E coli infection. We found that both strains of E coli adhered in the ileum and caused diarrhea in pigs of both age groups. In control experiments, adherent bacteria were not seen in the ileum of pigs < 1 day old or 3 weeks old that were noninoculated or inoculated with a nonpathogenic strain of E coli. These control pigs did not develop diarrhea. Antisera raised against strains 2134 and 2171 and absorbed with the autologous strain, grown at 18 C, were used for bacterial-agglutination and colony-immunoblot assays. Both absorbed antisera reacted with strains 2134 and 2171, but not with strains that express K99, F41, or 987P adhesins. A cross-reaction was observed with 2 wild-type K88 strains, but not with a K12 strain that expresses K88 pili. Indirect immunofluorescence with these absorbed antisera revealed adherent bacteria in frozen sections of ileum from pigs infected with either strain. We concluded that these strains are pathogenic and express a common surface antigen that may be a novel adhesin in E coli strains that cause diarrhea in weaned pigs.
اظهر المزيد [+] اقل [-]Spontaneous lingual papillomas in fischer 344 rats.
1992
Kang B.H. | Lim C.H.
Ultrastructure of virus particles in the liver of piglets infected with porcine enterovirus serotype 3.
1992
Shin T.K | Lee C.S. | Huh M.D.
Reverse effects of electroacupuncture on xylazine analgesia in goats.
1992
Seo K.M. | Yoon J.H. | Cheong J.T. | Chang K.H. | Nam T.C.
Isolation and morphological characterization of natural killer cell in the Sprague-dawley (SD) rats.
1992
Kang K.S. | Lee Y.S.
Comparison of soluble antigens Leptospira interrogans serovars by SDS-PAGE, crossed immunoelectrophoresis and immunoblotting.
1992
Baik Y.O. | Mah J.S.
Effect of Artemisia Iwayomogi water extract on hepatic injury by carbon tetrachloride in rats - (1) - Effect of serum AST, ALT, LDH activities, lipid content and liver peroxide content.
1992
Kim K.S. | Park J.H.
Parthenogenetic development of mouse eggs-(1)-Parthenogenetic activation by ethanol and hyaluronidase treatments.
1992
Lee H.J. | Kang T.Y. | Choi M.C. | Ha D.S.
Laboratory cultivation of blue-green algae for use as a food for Lymnaeids, the intermediate host of Fasciola hepatica.
1992
Lee C.G. | Kim S.K. | Lee C.Y.
Effect of bilateral stimulation on spinal evoked potentials in dogs النص الكامل
1992
Poncelet, Luc | Delauche, A. | Vinals, C. | Balligand, Marc
peer reviewed
اظهر المزيد [+] اقل [-]