خيارات البحث
النتائج 61 - 70 من 461
EVALUATION OF THE IMMUNE RESPONSES INDUCED BY EXPERIMENTAL INFECTION OF (BALB/c) MICE WITH SALMONELLA HADAR النص الكامل
2010
Afaf Abdul Rahman Yousif | Maan M. Neima AL-Naqeeb
The present study bases on evaluate the immune responses due to experimental infection of (BALB/c) mice by Salmonella hadar . The experiment was carried out on eighty mice of both genders with age range (6 – 8) weeks old, the mice were divided randomly into three groups (group A:- contain 20 mice were administrated orally with infectious dose (1.5×107C.F.Uml) ,group B:- contain 40 mice were administrated orally with LD50dose (1.5×109 C.F.Uml) and groupC:- contain 20 mice which inoculated orally with 1 ml of PBS (pH=7.2) and consider as control group). The study has noticed that the experimentally infected mice are able to induce humoral immune response which represented by producing antibody against Salmonella hadar and this production was elevated after two weeks of administration and reach the peak after four weeks post infection then decline sharply after passage of six weeks post infection in both groups (A and B) but the titration of the antibodies in group B was higher on that recorded in group A. It is obvious that S. hadar is able to induce cellular immune response during experimental infection with infectious dose and LD50 dose and the results of delayed type hypersensitivity have showed increases in the thickness of the right footpads of the mice of both groups (A and B) and the highest mean of the thickness was after 24 hours post immunization. Finally, we concluded that Salmonella hadar in infected the host was able to induce both humoral and cellular immune responses and these responses are dose dependent.
اظهر المزيد [+] اقل [-]Seroprevalence survey of <i>Chlamydophila abortus</i> infection in breeding goats on commercial farms in the Otavi Veterinary District, northern Namibia النص الكامل
2010
Alaster Samkange | Tendai C. Katsande | Georgina Tjipura-Zaire | Jan E. Crafford
A total of 1 076 sera from breeding goats were randomly collected from 24 different farms and tested with CHEKIT®-ELISA (IDEXX Laboratories B.V., 1 119 NE Schiphol-Rijk, Nederland) for antibodies against Chlamydophila abortus. The farms were divided into two categories of twelve farms each,based on their previous history of observed abortions over the previous 12 months: those with low (< 5%) levels of abortion and those with high (≥ 5%) levels of abortion. The farmers were also interviewed on their level of awareness about chlamydophilosis, its zoonotic importance and vaccination measures against the disease. The study detected overall seroprevalence levels of 25% for the farms and 8% for the individual animals (at 95% confidence). A total of six out of twentyfour farms (25%) had at least one positive breeding animal. Only five out of the twenty-four (20.8%)farmers interviewed were aware of chlamydophilosis and its zoonotic dangers. None of the 24 farmers interviewed practised any vaccination against chlamydophilosis. There was a significantly higher number of seropositive animals from farms with high levels of abortion, compared to those animals from farms with low levels of abortion (p = 0.0001). This study underscores the need for a higher level of farmer awareness and training on chlamydophilosis and its zoonotic dangers.
اظهر المزيد [+] اقل [-]Comparative ultrastructure of fibrin networks of a dog after thrombotic ischaemic stroke النص الكامل
2010
Etheresia Pretorius | Ursula B. Windberger | Hester M. Oberholzer | Roland E. Auer
Comparative ultrastructure of fibrin networks of a dog after thrombotic ischaemic stroke النص الكامل
2010
Etheresia Pretorius | Ursula B. Windberger | Hester M. Oberholzer | Roland E. Auer
A cerebrovascular accident or stroke is a rare condition in dogs, but previous studies suggest that it is now increasingly being recognised. Platelets and fibrin networks are involved in haemostasis,which is disrupted during a thrombotic event. In this study we investigate the ultrastructure of the fibrin networks of a dog that had suffered ischaemic stroke, following suspected thromboembolism from clots that became dislodged during catheter maintenance (flushing with heparinised saline) 2 days after carotid artery catheter insertion. Fibrin networks of blood samples that were collected immediately after the stroke, 15 min after treatment with streptokinase and 24 h after treatment, were studied. The results were compared to those of two control dogs. During a stroke, fibrin morphology changes to form a thick, matted layer.Post-treatment ultrastructure shows that the fibrin morphology returns to that comparable to controls. Our results show that during thrombotic risk, fibrin network morphology changes visibly and reduces the fibrinolytic activity of the coagulation system.
اظهر المزيد [+] اقل [-]Comparative ultrastructure of fibrin networks of a dog after thrombotic ischaemic stroke النص الكامل
2010
Pretorius, Etheresia(University of Pretoria Department of Anatomy Faculty of Health Sciences) | Windberger, Ursula B.(Medical University Vienna Center for Biomedical Research) | Oberholzer, Hester M.(University of Pretoria Department of Anatomy Faculty of Health Sciences) | Auer, Roland E. J.(University of Pretoria University of Pretoria Biomedical Research Centre)
A cerebrovascular accident or stroke is a rare condition in dogs, but previous studies suggest that it is now increasingly being recognised. Platelets and fibrin networks are involved in haemostasis, which is disrupted during a thrombotic event. In this study we investigate the ultrastructure of the fibrin networks of a dog that had suffered ischaemic stroke, following suspected thrombo-embolism from clots that became dislodged during catheter maintenance (flushing with heparinised saline) 2 days after carotid artery catheter insertion. Fibrin networks of blood samples that were collected immediately after the stroke, 15 min after treatment with streptokinase and 24 h after treatment, were studied. The results were compared to those of two control dogs. During a stroke, fibrin morphology changes to form a thick, matted layer. Post-treatment ultrastructure shows that the fibrin morphology returns to that comparable to controls. Our results show that during thrombotic risk, fibrin network morphology changes visibly and reduces the fibrinolytic activity of the coagulation system.
اظهر المزيد [+] اقل [-]Expression of vascular endothelial growth factor and endothelial nitric oxide synthase is increased in the placenta of sheep at high altitude in the Andes النص الكامل
2010
Parraguez, Victor H. | Atlagich, Miljenko A. | Urguieta, Bessie | Galleguillos, Marco | Reyes, Monica De los | Kooyman, David L. | Araneda, Silvia | Raggi, Luis A.
Expression of vascular endothelial growth factor and endothelial nitric oxide synthase is increased in the placenta of sheep at high altitude in the Andes النص الكامل
2010
Parraguez, Victor H. | Atlagich, Miljenko A. | Urguieta, Bessie | Galleguillos, Marco | Reyes, Monica De los | Kooyman, David L. | Araneda, Silvia | Raggi, Luis A.
Fetal weight and the placenta of sheep at high altitude (HA) are affected by hypoxia. Placental changes (an increase in placental size and vascularization) are greater in ewes from populations that have lived for several generations at HA than in those exposed during just 1 gestation. This study investigated placental expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), 2 molecules involved in placental angiogenesis that could be upregulated by hypoxia. Two groups of ewes were maintained at HA (3589 m) during pregnancy: HA-native ewes (group HH) and ewes native to lowlands but moved to HA immediately after the diagnosis of pregnancy (group LH). A control group (LL) was kept at sea level. Near term, placentomes were removed, weighed, and processed for immunohistochemical detection of VEGF and eNOS, as well as for vascular area measurement. Placental weight was significantly higher in the HH group than in the LH and LL groups; between the latter 2 groups there was no significant difference. The placental area occupied by vasculature was significantly greater in both the HA groups than in the LH group; the number of placentomes was greatest in the LL group. The density of VEGF and eNOS in the placentome tissue was significantly greater in both HA groups than in the LL group. Although the density of VEGF was significantly lower in the HH group than in the LH group, no differences were observed in eNOS density between the HH and LH animals. These results demonstrate that chronic hypoxia upregulates the expression of placental VEGF and eNOS, suggesting an important role of these molecules in the placental response to HA hypoxia. In addition, an attenuated response to hypoxia in VEGF synthesis may be part of the long-term process of adaptation to HA.
اظهر المزيد [+] اقل [-]Expression of vascular endothelial growth factor and endothelial nitric oxide synthase is increased in the placenta of sheep at high altitude in the Andes النص الكامل
2010
Parraguez Gamboa, Víctor | Miljenko, Atlagich | Urquieta, Bessie | Galleguillos, Marco | Reyes Solovera, Mónica de los | Kooyman, David L. | Araneda, Silvia | Raggi Saini, Luis
Artículo de Publicación ISI | Fetal weight and the placenta of sheep at high altitude (HA) are affected by hypoxia. Placental changes (an increase in placental size and vascularization) are greater in ewes from populations that have lived for several generations at HA than in those exposed during just 1 gestation. This study investigated placental expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), 2 molecules involved in placental angiogenesis that could be upregulated by hypoxia. Two groups of ewes were maintained at HA (3589 m) during pregnancy: HA-native ewes (group HH) and ewes native to lowlands but moved to HA immediately after the diagnosis of pregnancy (group LH). A control group (LL) was kept at sea level. Near term, placentomes were removed, weighed, and processed for immunohistochemical detection of VEGF and eNOS, as well as for vascular area measurement. Placental weight was significantly higher in the HH group than in the LH and LL groups; between the latter 2 groups there was no significant difference. The placental area occupied by vasculature was significantly greater in both the HA groups than in the LH group; the number of placentomes was greatest in the LL group. The density of VEGF and eNOS in the placentome tissue was significantly greater in both HA groups than in the LL group. Although the density of VEGF was significantly lower in the HH group than in the LH group, no differences were observed in eNOS density between the HH and LH animals. These results demonstrate that chronic hypoxia upregulates the expression of placental VEGF and eNOS, suggesting an important role of these molecules in the placental response to HA hypoxia. In addition, an attenuated response to hypoxia in VEGF synthesis may be part of the long-term process of adaptation to HA. | The authors thank Gabino Llusco and Enrique Perez for assistance with animal management and care. This study was funded by grants ENL 06/2 (from Direction of Research and Development, DID, University of Chile) and FONDECYT 1070405 (from National Agency for Scientific Research, CONICYT).
اظهر المزيد [+] اقل [-]Parasites of domestic and wild animals in South Africa. XLVIII . Ticks (Acari: Ixodidae) infesting domestic cats and wild felids in southern Africa النص الكامل
2010
Ivan G. Horak | Heloise Heyne | Edward F. Donkin
Ticks collected from domestic cats (Felis catus), cheetahs (Acinonyx jubatus),caracals (Caracal caracal), African wild cats (Felis lybica), black-footed cats (Felis nigripes), a serval (Leptailurus serval), lions(Panthera leo), and leopards (Panthera pardus) were identified and counted. Thirteen species of ixodid ticks and one argasid tick were identified from domestic cats and 17 species of ixodid ticks from wild felids. The domestic cats and wild felids harboured 11 ixodid species in common. The adults of Haemaphysalis elliptica, the most abundant tick species infesting cats and wild felids, were most numerous on a domestic cat in late winter and in mid-summer, during 2 consecutive years. The recorded geographic distribution of the recently described Haemaphysalis colesbergensis, a parasite of cats and caracals, was extended by 2 new locality records in the Northern Cape Province,South Africa.
اظهر المزيد [+] اقل [-]Incidence of hoof affections in equine النص الكامل
2010
M. M. Seif | A. M. Sedeek | M. Z. Fathy | G. A. Ragab
This study was conducted on 1178 lamed animals of equine species. Cases were classified according to the species, affected part of the foot, and nature of the affection. Hoof affections represented in about 722 animals with 61.3 % of total lame animals. The percentages of hoof affections were higher in forefeet than hind feet. The present study revealed that sandy cracks, toe- in and long toe were the predominant hoof wall affections. The corn was the highest sole affections followed by white line disease and the lowest percentages were flat foot and bruised sole. The highest affection percentage in frog was thrush followed by picked up nail and the lowest was in under run heel.
اظهر المزيد [+] اقل [-]Radiologic and ultrasonographic studies of kidneys in goat النص الكامل
2010
G. A. Ragab | M. M. Seif | U. A. Hagag
A study was performed on fifty normal goats for radiographic (only 20 animals), and sonographic (the all 50 animals) investigations. Radiographic results revealed that, both kidneys had bean shaped and smooth contour. The right kidney position was constant due to its relation with the liver, while, the left kidney was mobile and its position varied according to the degree of fullness of the rumen due to its long mesentery. The sonographic results revealed the position, shape, dimensions and echogenicity of kidneys, by using 3.5/5.0 MHz curved transducer and 6.0/8.0 MHz linear transducer. The renal capsule was represented by a very fine echogenic lines around the kidney that couldn’t be distinguished from the peri-renal fat. The cortex was more echogenic than the medulla and had uniform finely granular hypoehoic pattern. The corticomedullary junction was slightly more echogenic than the cortex due to the presence of the arcuate arteries. The medulla was observed as hypoechoic area interrupted by bright echogenic lines representing the recesses and interlobular arteries that gave the appearance of typical medullary pyramids. The dimensions of the right kidney were 5.32 ± 0.12, 2.79 ± 0.07, 0.64 ± 0.01, 1.52 ± 0.03, 2.74 ± 0.09, 1.27 ± 0.06, 1.25 ± 0.05, and 1.72 ± 0.09; and the dimensions of the left kidney were 5.34 ± 0.12, 2.80 ± 0.07, 0.65 ± 0.01, 1.55 ± 0.03, 2.76 ± 0.09, 1.26 ± 0.06, 1.28 ± 0.05, and 1.73 ± 0.1, for the length, width, cortical thickness, medullary thickness, depth, diameter of renal sinus, diameter of renal parenchyma, and circumference of renal pyramids respectively.
اظهر المزيد [+] اقل [-]Hormonal residues in chicken carcasses النص الكامل
2010
F. A. Khalafalla | E. M. El-Neklway | Hala A. M. Ibrahim
Ninety chicks were experimentally, orally received different doses of estradiol-17β and diethylstilbestrol with ration. Samples were collected from tissues (wings, breast muscles, thigh muscles, skin and fat) and giblets (liver and gizzard) for detection of hormonal residues after 4 and 21 days from the last dose, which proved the presence of such residues in all samples. Effect of temperatures (boiling, roasting and freezing) on hormonal residues of positive samples was evaluated. It was proved that There is no significant variations in reduction of hormonal residues in each of breast and thigh muscles of chickens at (p < 0.05) after boiling, roasting and freezing at - 20˚ C as well as a significant differences was detected in skin and fat samples at (p < 0.05) after boiling and roasting. Public health importance of hormonal residues was discussed.
اظهر المزيد [+] اقل [-]Influence of water quality on fish productivity النص الكامل
2010
M. A. El Bably | H. H. Emeash | Asmaa N. Mohamed | Nahla R.
The physical, chemical and bacteriological parameters of water in fish ponds were investigated with a view to optimize the conditions for fish productivity by using three private fish farms with different water supplies. Water and fish samples were collected equally from each pond over a period of 17 months. Water temp., Dissolved oxygen (DO), pH, Ammonia, Nitrite, Nitrate, and bacterial count were determined. The average values of bacterial, parasitic, survival and growth rates of fish were also assessed. Results revealed that pond water in farm (III) had the highest averages of temperature nitrite, nitrate 31.8±1.5, 8.18± 0.9, 0.41 ± 0.06 , 3.79 ± 0.6 resp., with the least content of DO 3.6 ± 0.7 followed by farm (II),which had also the highest mean values of NH3 - N, total coliform and fecal coliforms were 3.15±0.65, 59.0 & 18.5 followed by ponds of farm (III), while, the lowest averages of estimated parameters were recorded in farm (I).Correspondingly, fish samples of farm (III) showed the highest level of total bacterial, coliform, fecal coliform count and parasitic infestation; were 42.5±5.4, 29.6±3.6 , 11.3±3.1 and 31.15% respectively, followed by fish of farm(II) 29.75±3.5, 11.5±3.3, 7.4±1.1 and 10.8 % respectively, Meanwhile, fish in farm (I) showed the lowest value of both bacterial contents with no parasitic infestation which explain the highest percentages of daily weight gain & survival rates 35.2±4.4 gm. & 97.0 ±1.6 % respectively. Throughout study period compared with those in farms (III & II) respectively, resulting from poor water quality & parasitic infestation which acts as stressors affecting fish health and productivity. Therefore, fish productivity can be enhanced if the water quality in the ponds were maintained at optimum levels.
اظهر المزيد [+] اقل [-]Some laboratory studies on freshwater snails and the effect of some plants on their biological control in Beni-Suef governorate النص الكامل
2010
M. A. Abdel-Gawad | A. M. Khateeb
Freshwater snails collected from ditch like ponds near the River Nile in Beni-Suef Governorate during three climatically differed periods. Lymnaea cailliaudi, Biomphalaria alexandarina, Bulinus truncatus, Lanistes carinatus, Cleopatra bulimoids,Physa acuta, Viviparus viviparus, Melania tuberclata and Bivalves species were included. The total number of collected snails in December 2008, March 2009 and July 2009 were 1321, 1370 and 1211 respectively, with larger number in March than in July or December due to optimal climatic conditions in Marsh for most of collected snails. The recorded survival longevity in the laboratory was more than 90 days for Lymnaea cailliaudi, two months for Lanistes carinatus, while in Melania tuberclata, was about three weeks but only two to three days for Bivalves. Cercariae shedding observed from Lymnaea, Lanistes and Biomphalaria species beside stylet cecariae from Lymnaea and Lanistes snails, also rediae and sporocysts were found in crushed snails in all periods of collections with little difference between these periods, about 14% of crushed Lymnaea were containing rediae. Aquatic insects and their larvae were found especially water bug Sphaerodema sp. found predating snails or their eggs, also the mature insect still alive in the breeding container all over the period of the experiment more than three months. Culex larvae attacked egg masses of the snails, estacosa predated the snails while Telapia nilotica fishes showed low affinity to predate such snails. The oleo-gum resin Myrrh (Commiphora molmol) in the concentration of 0. 5gm/1 litre water killed 90 % of the exposed snails after 72 hours.
اظهر المزيد [+] اقل [-]