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An investigation into the possibility of bluetongue virus transmission by transfer of infected ovine embryos النص الكامل
2011
Estelle H. Venter | Truuske Gerdes | Isabel Wright | Johan Terblanche
An investigation into the possibility of bluetongue virus transmission by transfer of infected ovine embryos النص الكامل
2011
Estelle H. Venter | Truuske Gerdes | Isabel Wright | Johan Terblanche
Bluetongue (BT), a disease that affects mainly sheep, causes economic losses owing to not only its deleterious effects on animals but also its associated impact on the restriction of movement of livestock and livestock germplasm. The causative agent, bluetongue virus (BTV), can occur in the semen of rams and bulls at the time of peak viraemia and be transferred to a developing foetus. The risk of the transmission of BTV by bovine embryos is negligible if the embryos are washed according to the International Embryo Transfer Society (IETS) protocol. Two experiments were undertaken to determine whether this holds for ovine embryos that had been exposed to BTV. Firstly, the oestrus cycles of 12 ewes were synchronised and the 59 embryos that were obtained were exposed in vitro to BTV-2 and BTV-4 at a dilution of 1 x 102.88 and 1 x 103.5 respectively. In the second experiment, embryos were recovered from sheep at the peak of viraemia. A total of 96 embryos were collected from BTV-infected sheep 21 days after infection. In both experiments half the embryos were washed and treated with trypsin according to the IETS protocol while the remaining embryos were neither washed nor treated. All were tested for the presence of BTV using cell culture techniques. The virus was detected after three passages in BHK-21 cells only in one wash bath in the first experiment and two unwashed embryos exposed to BTV-4 at a titre of 1 x 103.5. No embryos or uterine flush fluids obtained from viraemic donors used in the second experiment were positive for BTV after the standard washing procedure had been followed. The washing procedure of the IETS protocol can thus clear sheep embryos infected with BTV either in vitro or in vivo.
اظهر المزيد [+] اقل [-]An investigation into the possibility of bluetongue virus transmission by transfer of infected ovine embryos النص الكامل
2011
Venter, Estelle H(University of Pretoria Department of Veterinary Tropical Diseases) | Gerdes, Truuske(University of Pretoria Department of Theriogenology) | Wright, Isabel(University of Pretoria Virology Section) | Terblanche, Johan(University of Pretoria Department of Theriogenology)
Bluetongue (BT), a disease that affects mainly sheep, causes economic losses owing to not only its deleterious effects on animals but also its associated impact on the restriction of movement of livestock and livestock germplasm. The causative agent, bluetongue virus (BTV), can occur in the semen of rams and bulls at the time of peak viraemia and be transferred to a developing foetus. The risk of the transmission of BTV by bovine embryos is negligible if the embryos are washed according to the International Embryo Transfer Society (IETS) protocol. Two experiments were undertaken to determine whether this holds for ovine embryos that had been exposed to BTV. Firstly, the oestrus cycles of 12 ewes were synchronised and the 59 embryos that were obtained were exposed invitro to BTV-2 and BTV-4 at a dilution of 1 x 10(2.88) and 1 x 10(3.5) respectively. In the second experiment, embryos were recovered from sheep at the peak of viraemia. A total of 96 embryos were collected from BTV-infected sheep 21 days after infection. In both experiments half the embryos were washed and treated with trypsin according to the IETS protocol while the remaining embryos were neither washed nor treated. All were tested for the presence of BTV using cell culture techniques. The virus was detected after three passages in BHK-21 cells only in one wash bath in the first experiment and two unwashed embryos exposed to BTV-4 at a titre of 1 x 10(3.5). No embryos or uterine flush fluids obtained from viraemic donors used in the second experiment were positive for BTV after the standard washing procedure had been followed. The washing procedure of the IETS protocol can thus clear sheep embryos infected with BTV either in vitro or in vivo
اظهر المزيد [+] اقل [-]On the origin and diversity of Newcastle disease virus in Tanzania النص الكامل
2011
Mmeta G. Yongolo | Henrik Christensen | Kurt Handberg | Uswege Minga | John E. Olsen
On the origin and diversity of Newcastle disease virus in Tanzania النص الكامل
2011
Mmeta G. Yongolo | Henrik Christensen | Kurt Handberg | Uswege Minga | John E. Olsen
Free-range rural chickens (FRCs) dominate the poultry industry in developing countries and chickens are exposed to multi-host infections, including Newcastle disease virus (NDV). The knowledge about the characteristics of NDV from FRCs is limited. This study investigated the persistence, spread and risks of NDV from FRCs. NDV isolates (n = 21) from unvaccinated FRCs in Tanzania were characterised by conventional intracerebral pathogenicity index (ICPI) and sequence analysis of a partial region of the deduced fusion protein encompassing the cleavage site. Results showed that five isolates were screened as lentogenic, nine as mesogenic and six as velogenic. Phylogenetic analysis of the 21 isolates compared to reference sequences revealed three, four, nine and five isolates in genotypes 1, 2, 3c and 4a, respectively. Genotype 3c also included published sequences of Tanzanian isolates obtained from exotic birds and chicken isolates from Uganda. The analysis showed that NDV were persistently present among chicken populations and possibly spread through live chicken markets or migration of wild birds. Differences in amino acid sequences detected around the cleavage site separated the isolates in six types. However, cleavage site pattern could not fully differentiate mesogenic isolates from velogenic isolates.
اظهر المزيد [+] اقل [-]On the origin and diversity of Newcastle disease virus in Tanzania النص الكامل
2011
Yongolo, Mmeta G.(Ministry of Livestock Development Department of Virology) | Christensen, Henrik(University of Copenhagen Department of Veterinary Disease Biology) | Handberg, Kurt(University Hospital of Aarhus Department of Clinical Microbiology) | Minga, Uswege(Open University of Tanzania Faculty of Science, Technology and Environmental Studies) | Olsen, John E.(University of Copenhagen Department of Veterinary Disease Biology)
Free-range rural chickens (FRCs) dominate the poultry industry in developing countries and chickens are exposed to multi-host infections, including Newcastle disease virus (NDV). The knowledge about the characteristics of NDV from FRCs is limited. This study investigated the persistence, spread and risks of NDV from FRCs. NDV isolates (n = 21) from unvaccinated FRCs in Tanzania were characterised by conventional intracerebral pathogenicity index (ICPI) and sequence analysis of a partial region of the deduced fusion protein encompassing the cleavage site. Results showed that five isolates were screened as lentogenic, nine as mesogenic and six as velogenic. Phylogenetic analysis of the 21 isolates compared to reference sequences revealed three, four, nine and five isolates in genotypes 1, 2, 3c and 4a, respectively. Genotype 3c also included published sequences of Tanzanian isolates obtained from exotic birds and chicken isolates from Uganda. The analysis showed that NDV were persistently present among chicken populations and possibly spread through live chicken markets or migration of wild birds. Differences in amino acid sequences detected around the cleavage site separated the isolates in six types. However, cleavage site pattern could not fully differentiate mesogenic isolates from velogenic isolates.
اظهر المزيد [+] اقل [-]Effect of acute exposure of lead acetates on the morphology of liver and kidney of mice (Mus musculus) and the role of vitamin C as a repairing agent النص الكامل
2011
Ishraq J. H | Wissam S.H. Al-Uboody | M.A. Al-Diwan
The objective of this study is to elicit the destructive effect of lead on the tissues of liver and kidney of mice and if vitamin C is capable of repairing the damage caused by lead. In this study, 40 male mice were used and divided into three groups as: Control group which consists of 16 mice; 8 of them were injected intraperitoneally with (0.9 % N.S.) for 15 days daily then they were sacrificed and the remainder 8 mice were injected intraperitoneally with (0.9 % N.S.) for additional 15 days daily then they were sacrificed, First treated group (T1 group) which consists of 8 mice only; they were injected intraperitoneally with (80 mg/kg) lead acetates then with (400 mg/kg) vitamin C after one hour of lead acetates injection for 15 days daily then they were sacrificed, Second treated group(T2 group) which consists of 16 mice; they were injected intraperitoneally with (80 mg/kg) lead acetates for 15 days daily then 8 of them were sacrificed and called as (T2a) and the remainder 8 mice were injected with (400 mg/kg) vitamin C for additional 15 days daily and called as (T2b). Histologically, the kidneys of the lead acetates treated group indicated undefined epithelial cell lining and also the presence of giant-like cells. When vitamin C offered, it decreased the damage that caused by lead where the kidneys indicated the presence of cuboidal epithelial cells with disrupted epithelial cell lining, and increased intracellular space in the lumen. The livers of the lead acetates treated group indicated the presence of abnormal hepatocytes with distorted shape and undefined epithelial cell lining enlarged nucleus with vacuolations. The incidence of changes and severity were less in the vitamin C treated group. The effect of vitamin C was similar if it is offered after one hour of lead injection or for additional 15 days after lead injection. Hence acute exposure to lead causes morphological changes in the liver and kidney of mice. Hence acute exposure of lead may be toxic and is associated with various pathological conditions such as hepatic and renal dysfunction and cancers.
اظهر المزيد [+] اقل [-]EFFECT OF VITAMIN C ON APOPTOTIC GERM CELLS OF CRYPTORCHID TESTIS IN RABBITS النص الكامل
2011
F.S. AL-Asadi
The study examined the effect of vitamin C on apoptotic testicular germ cells in experimentally induced cryporchidism in the male rabbits .Oral administration of vitamin C (10 mg. kg body weight)for 8 weeks to rabbits showed significant elevation in testis parameters in treated cryptorchid groups (TC), also significantly elevated the number of germ cells compared to un treated cryptorchid groups (CC).However, vitamin C caused significant depression in apoptotic germ cells and apoptotic tubules in treated cryptorchid groups (TC) compared to un treated cryptorchid groups (CC). vitamin C alleviated the deleterious effect of oxidative stress in cryporchidism
اظهر المزيد [+] اقل [-]PATHOLOGICAL EFFECT OF TOXOCARA CANIS EGG DOSES IN EXPERIMENTAL MICE. النص الكامل
2011
Hind J. Mahmood | Wisam M. Al-Saeed
In this study we examined the effect of various sensitizing doses of infective Toxocara canis eggs and the trapping of larvae in different tissues of the murine host. The level of trapping increased with sensitization egg doses. Different doses of Toxocara canis eggs were given to ten groups each with five mice and then necropsied after different periods. Experimental toxocariasis in mice sacrificed at (1,2 and 3) weeks post infection showed no lesions in mice given 50, 75 and 100 Toxocara canis eggs respectively. Other groups given higher doses of T.canis eggs between (125-500 eggs) showed lesions in liver, lungs, eyes and muscles of sacrificed mice after 4 weeks of infection.
اظهر المزيد [+] اقل [-]EFFCT OF L-ARGININE ON SPERMATOGENESIS OF THE DIABETIC RAT النص الكامل
2011
Eyhab.R.M.AL-Samawy | Ahmed Sami Jarad | Ahmed Shaker Hisab ALBadran
L-Arginine has hypoglycemic and antioxidant effect in Alloxan diabetic animals and reduce effect of diabetes complication on spermatogenesis. antioxidant have essential effect on spermatogenesis, L-Arginine has antioxidant and hypoglycemic effect. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Sixteen mature male rats aged 10 weeks ,were randomly divided into four equal groups as follows 1st diabetic group (DG) that received 150 mg/kg (IP) Alloxan single dose ;2nd group diabetic group treated with (L-Arginine-Hcl)(DAG) received 150mg/kg (IP) Alloxan as single dose plus L-Arginine-Hcl 200mg/kg(IP)-per day, 3ed group treated with(L-Arginine-Hcl)(AG) received 200mg/kg-(IP) perday ,and 4th were control group(CG) non treated .In 60 day the blood samples collected from heart to make serological parameters (glucose level) and testes removed to observe their Histopathological. Serum Glucose concentration showed a significant increase (p
اظهر المزيد [+] اقل [-]ANTIOXIDANT, ANTIDIABETIC AND LIPID LOWERING EFFECTS OF CINNAMON AND VITAMIN C IN HYPERGLYCEMIC RABBITS النص الكامل
2011
Ahlam A. Al-Rikaby | and Kassim F. Abdul Kareem | Ala Al-Deen H. Jawad
The study was done to evaluate the antioxidant effects of cinnamon and vitamin C in controlling hyperglycemia and their effect on lipid profile in male rabbits in comparison with the effects of insulin therapy and control animals. Twenty four diabetic rabbits by the injection of alloxan 100 mg/kg body weight in the marginal vein of the ear. These diabetic rabbits were divided randomly into 4 groups Number of animals in each group = 6: Group 1: Was given 2 I.U/ animal of insulin subcutaneously daily. Group 2: Was given ground cinnamon orally 300 mg/kg body weight dissolved in 5 ml normal saline daily. Group 3: Was given vitamin C orally 200 mg/kg body weight dissolved in 5 ml normal saline daily. Group 4: Received orally 5 ml normal saline (0.9% Nacl) daily and considered as control group. All animal groups were treated for five weeks. Blood samples were taken from these groups weekly for biochemical analysis to estimate: Blood glucose, Lipid profile (include total cholesterol (TC), triglyglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein(VLDL) and serum malondialdehyde (MDA). The results showed high glucose and lipid concentration associated with an increased oxidant stress alloxan induces on diabetic animals. The statistically analysis showed that a cinnamon and vitamin C significant (P< 0.05) reduction in glucose and lipid profile (TC, TG, HDL, LDL and VLDL) in concordance with a significant elevation in HDL (P
اظهر المزيد [+] اقل [-]A STUDY ON BLOOD PARAMETERS OF BARBUS XANTHOPTERUS , BARBUS SHARPEYI AND THEIR HYBRID النص الكامل
2011
Abed Al. Majeed H. Talal | Jassim H. Saleh. Amer A. .Jabir
The blood parameters of Red Blood Cells for Barbus xanthopterus, Barbus sharpeyi and B. xanthopterus X B sharpeyi (hybrid )were mentionted. The dimentions of Red Blood Cells ( length and width) for the fish B.xanthopterus, B.sharpeyi and their hybrid were as follows: (6 and 4 microns; 5 and 3 microns; 6 and 4 microns). Hb content values for the above mentioned fish, were as follows: 5.18, 5.323 and 4 g /100 ml. PCV values for the above mentioned fish were as follows: 68.25% , 100% and 42.85714%. RBC numbers for the above fish were also mentioned as follows: 3450000, 3550000 and 3300000cell/1mm3.
اظهر المزيد [+] اقل [-]ISOLATION AND BIOTYPING OF STAPHYLOCOCCUS AUREUS FROM WHITE CHEESE IN BASRAH LOCAL MARKETS النص الكامل
2011
Nawras N. Jaber
Fifty samples of white cheese were collected from 3 different local market of Basra city AL-basra, (15), AL-ashar(20) and AL-jumhurya(15 ) samples respectively, After being examined by culturing on MSA media , the results reveal thate 53.33% , 50% , and 13.33% of Staph aureus were isolate from white chees respectively . Depending on the biotyping , the percentage of biotype A Staphylococcus aureus was 90% and biotype C was 10% . Antibiotic sensitivety test showed that 55% ,55% , 45% , 35% of isolates were more sensitive to Erythromycin Chloramphenicol Tetracycline and Ciprofloxacin respectively
اظهر المزيد [+] اقل [-]IN VITRO AND IN VIVO STUDY OF GREEN AND BLACK TEA ANTIMICROBIAL ACTIVITY ON METHICILLIN RESISTANT STAPHYLOCOCUS AUREUS النص الكامل
2011
Raied Taha Yassen | Lammya Kadhum Bakkir | Reham Muhammed
The in vitro study was compared the antibacterial activity of green tea and black tea extracts against methicillin resistant Staphylococcus aureus (MRSA). Green tea extract was found to have higher antimicrobial activity on MRSA with an inhibition zone of 20 mm at a concentration of 10 gm/100 ml. For black tea extract, the inhibition zone was 15 mm at same concentration .The minimum inhibitory concentrations for the green tea and black tea were 4mg/ml and 8mg/ml respectively. The in vivo study of the antimicrobial effect of both green and black tea was investigated, by subcutaneous inoculation of four rabbits with MRSA bacteria. After that immediately treated with a series dilution of tested material (green and black tea), the development of swelling and the degree of necrosis were scored. The green tea showed marked reduction in the size of swelling less than 2mm at the concentration of 10mg/ ml; also there is a marked reduction in the severity of necrosis. For black tea a marked reduction occur in the size of swelling (6-10mm) at the concentration of 10mg/100ml; also there is a marked reduction in the severity of necrosis at the same concentration. This study concludes that green and black tea extracts were showed to have an antibacterial activity against MRSA and a therapeutic effect against skin infection caused by MRSA in rabbits
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