Objective: The aim of this study is to determine narrowband UVB (NB-UVB) irradiation's effect on the promotion of skin cancer, particularly its effect on DNA damage, oxidative stress, inflammation, and histological changes in Wistar rat skin. Materials and Methods: Wistar rats were selected for this study and randomly divided into control, dimethylbenzanthracene (DMBA), and DMBA+NB-UVB groups. The rats were given a single dose of DMBA and exposed to NB-UVB 3 times a week for 10 weeks. The radiation dose started with 1 minimal erythema dose, which is equivalent to 3.192 J/cm². In the 11th week, analysis on cyclobutene pyrimidine dimer (CPD), malondialdehyde (MDA), nuclear factor kappa-B (NFκB), inflammatory cytokines, and histopathology examination of the skin tissue was conducted. Results: Higher CPD, MDA, NFκB, tumor necrosis factor a (TNF-a), interleukin (IL)-6, IL-11, IL-10, and IL-12 levels in rats exposed to DMBA+NB-UVB for 10 weeks compared to control and DMBA groups. Macroscopic examination presented erythema, skin thickening, desquamation, ulcer, and crust. Histopathology examination showed hyperkeratosis, acanthosis, atypical keratinocytes,irregular arrangement of the basement membrane, and inflammatory cell infiltration in the DMBA+NB-UVB group. Conclusion: This research has shown that 10 weeks of a combination of DMBA and NB-UVB irradiation induced DNA damage, oxidative stress, inflammation, and histological changes in the Wistar rat skin. [J Adv Vet Anim Res 2024; 11(4.000): 1105-1113]

Objectives: The research is based on the assessment of the beneficial role of vitamin E (vit-E) supplementation on the reproductive and renal functions in Cadmium (Cd)-exposed male mice. Materials and Method: Mice (n = 15 in each group) were kept untreated (Group A) or fed with cadmium chloride (CdCl2) (3.5 mg/kg, Group B) per day or both CdCl2 (3.5 mg/kg) with vit-E supplementation (200 mg/kg, Group C) daily for 60 days. Mice were euthanized, blood samples were collected, and serum was prepared for biochemical and hormonal analysis. Sperm motility, sperm concentration, testis weight, and diameter were taken. Tissues from the kidneys and testicles were collected in 10% neutral buffered formalin for histotexture study. Results: Cd treatment reduced the serum thyroxine (T4) and testosterone levels, but vit-E supplementation increased both T4 and testosterone levels in the Cd-treated mice. Cd treatment decreased sperm motility and concentration, testicular weight, and diameter, and induced degenerative changes in the seminiferous tubules, which significantly improved upon vit-E supplementation. Increased serum urea, uric acid, and creatinine concentrations, along with cellular infiltration in the renal tubular epithelium and glomerular hyperplasia, were found in the Cd-treated mice, which were not found in the vit-E-supplemented mice. Conclusion: The study points to the harmful consequences of Cd on reproductive performance and renal functions that could potentially be mitigated upon vit-E supplementation in the diet. [J Adv Vet Anim Res 2024; 11(4.000): 1057-1065]

Objective: Many studies focused on clinical cases such as ovariohysterectomy of bitches and scarcely mentioned the histological features. The present study describes the cytoarchitecture characteristics of a local dog's mature adult reproductive tract. Materials and Methods: Sixteen samples of uterus and cervix were obtained from local breed bitches to conduct this study. The organs were processed according to routine histopathological protocol and stained with hematoxylin and eosin, Masson's trichrome, and combined Alcian blue (2.5 pH) and PAS (AB-PAS) stains. Results: The mature endometrium formed numerous short epithelial folds and epithelial crypts composed of mucous cells and cuboidal cells. The core of the endometrium is composed of fibrous tissue containing fibroblasts with discernible active uterine glands. The myometrium is constructed by thick, circularly, and longitudinally oriented layers of smooth muscle fibers. The AB-PAS stain of the uterine glands revealed light-neutral glycoprotein. The cervix had a very thick wall and displayed numerous huge mucosal folds, covered by ciliated and non-ciliated pseudo-stratified epithelium. The tissue core of the cervix was very thick, and composed of highly cellular, highly vascular, and non-glandular fibrous connective tissue. Layers forming the muscularis of the cervix are composed of circularly, obliquely, and longitudinally oriented smooth muscle bundles. The cervical epithelial cells revealed a light film of neutral glycoprotein that covered the epithelial surface, and the goblet cells denoted strong acidic mucopolysaccharide. Conclusions: The current study concluded that the most mature nonpregnant local breed dogs during the proestrus and estrus phases had an active endometrial architecture that is suitable for the management of reproduction. [J Adv Vet Anim Res 2024; 11(4.000): 835-845]

Objectives: The current study aimed to investigate the prophylactic potential of EBN compared to EDTA in mitigating Cd's toxic effects on pregnancy rates and embryonic development in rats. Materials and Methods: Ninety-eight female rats (Sprague Dawley) were divided into donor and recipient groups, with donors further divided into seven subgroups, including negative control, Cd-exposed, EBN-treated, and EDTA-treated groups. Embryos from donors were transferred to recipient rats, with EBN and Cd administered for 4 weeks and EDTA given only in the last 5 days for the donor group. Results: Results showed significant differences in pregnancy rates and blastocyst quality. EBN at 120 mg/kg BW led to higher blastocyst production and better quality compared to Cd-exposed groups. The highest pregnancy rates in recipient groups correlated with the highest blastocyst scores from donors. Conclusion: EBN at 120 mg/kg demonstrated significant protection against Cd toxicity and its effect on pregnancy rates, embryo production, quality, and pre- and post-embryo transfer, sur¬passing the effects of both 90 mg/kg EBN and EDTA. This study provides empirical evidence in support of the conventional belief in the positive impact of EBN on female reproduction. [J Adv Vet Anim Res 2024; 11(4.000): 944-953]

Objective: The study aimed to characterize frozen-thawed sperm proteins and their correlation with sperm kinematics, acrosome integrity, and mitochondrial membrane potential (MMP) in Indonesian Toraya buffalo bulls. Materials and Methods: Semen was obtained from six Toraya buffaloes classified as Saleko and Bonga 4–10 years old. The frozen semen was analyzed for sperm motility, sperm kinematics, viability, abnormalities, membrane integrity, intact acrosome, MMP, DNA integrity, and sperm protamine (PRM) deficiency. Sperm protein concentration (Prot. Con) was determined by the bicinchoninic acid, and protein molecular weight (MW) was determined using 1D sodium dodecyl sulfate-polyacrylamide gel electrophoresis with 4%–20% gradient gel and 6.5–240 kDa protein marker. Results: The results of this study showed that the quality of frozen semen from Toraya buffalo bulls is in a good category and suitable for use in artificial insemination programs. The sperm quality differed significantly (p < 0.05) between individuals, immunofluorescence examination of intact acrosome, PRM deficiency, intact MMP, and intact DNA showed no significant difference (p < 0.05). Pearson correlations in this study showed that sperm Prot. Con has a significant correlation (p < 0.05) with acrosome integrity. The sperm protein band (Prot. Band) correlated significantly (p < 0.05) with sperm kinematic parameters and intact MMP. The average sperm Prot. Con of Toraya buffalo was 77.29 ± 39.26 μg/ml and 4–13 Prot. Bands with 6–240 kDa of MW were detected, with Prot. Bands of 16, 50, 70, and 115 kDa having higher intensity. Conclusions: Frozen-thawed sperm protein is correlated with sperm kinematics, acrosome integrity, and MMP. The proteins were found to correlate with sperm quality and fertility in Toraya buffalo bulls. [J Adv Vet Anim Res 2024; 11(4.000): 869-879]

Objective: The study aimed to assess sperm motility characteristics, kinematic parameters, and sperm protein molecular weight (MW) in Indonesian buffalo to predict fertility. Materials and Methods: Frozen semen from Silangit (4 bulls), Murrah (4 bulls), and Toraya (2 bulls)—aged 7–10 years, was analyzed. Sperm motility was assessed using Computer Assisted Semen Analysis, viability and abnormality were evaluated using eosin-nigrosin staining, plasma membrane integrity was evaluated using the hypoosmotic swelling test, acrosomal status was evaluated using lectin peanut agglutinin, protamine deficiency was evaluated using chromomycin A3, and deoxyribonucleic acid (DNA) integrity was evaluated using Halomax. Protein concentra¬tion was determined using the bicinchoninic acid method and characterized with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results: The study revealed breed-specific variations in semen quality. Silangit buffaloes exhibited lower DNA integrity, while Murrah buffaloes showed elevated motility and membrane integrity. Toraya buffaloes displayed higher normal morphology and protamine status, though they had lower viability. Notable differences in protein expression included the presence of SPAG9 and the absence of IZUMO1 in Toraya buffaloes. Protein MW analysis further showed correlations with sperm characteristics. In Murrah buffaloes, proteins within the 130–125 kilodalton (kDa) range were negatively correlated with acrosome integrity, whereas in Toraya buffaloes, proteins within the 55–50 kDa range were negatively correlated with sperm abnormalities. Silangit buffaloes showed a positive correlation between proteins at 32 kDa and sperm abnormalities. Conclusion: Analyzing protein MW through SDS-PAGE provides a promising approach for assess¬ing semen quality in indigenous Indonesian buffalo bulls. Although the semen quality of the buf¬faloes in this study was variable, all bulls met the established Indonesian standards for semen quality and exhibited adequate fertilization potential. These results provide valuable insights into the reproductive biology of Indonesian buffalo bulls and form the basis for predicting fertility capacity through a comprehensive analysis of sperm characteristics and molecular profiles of sperm proteins. [J Adv Vet Anim Res 2024; 11(4.000): 846-855]

Objective: This study evaluated the immunogenicity and protective efficacy of an inactivated oil adjuvant B. abortus vaccine in BALB/c mice. Materials and Methods: Mice in group A (n = 30) received subcutaneous (s.c.) immunization with 0.1 ml of vaccine (1.5 × 107 inactivated B. abortus biovar 3 per mouse) and were boosted 4 weeks later. Group B (n = 30) received normal saline as unvaccinated controls. BALB/c, vaccinated and unvaccinated mice were challenged with B. abortus biovar 3 (3 × 107 cells per mouse) at 6 weeks post-vaccination (WPV). Serum antibody levels were assessed at 0, 1, 2, 3, 4, 5, and 6 WPV using RBPT and i-ELISA. Cellular-mediated immune (CMI) response was evaluated by measuring the skin thickness of vaccinated mice's left and right hind footpads sensitized with B. abortus soluble antigen and PBS, respectively. Bacterial persistence and spleen histopathological lesions were evaluated at 1, 2, and 3 weeks post-challenge. Results: The vaccinated mice developed B. abortus-specific serum IgG response from 2 WPV. The highest serum IgG titer was observed in 5-6 WPV (p < 0.001). The skin thickness was significantly higher in the left footpad than the right footpad (p < 0.001). Huge cellular infiltration with mononuclear and polynuclear cells was noticed in the dermis and sub-dermis areas of the left footpad. The spleen weight and bacterial load in the spleen were significantly reduced in vaccinated mice compared to unvaccinated control mice (p < 0.001). Conclusions: The inactivated oil adjuvant B. abortus vaccine induced both humoral and CMI responses, which conferred protection in BALB/c mice against virulent challenge infections. [J Adv Vet Anim Res 2024; 11(4.000): 914-923]

Objective: The aim of this study was to determine the best form and concentration of yogurt probiotics that can reduce leucocyte, neutrophil, lymphocyte, alkaline phosphatase, and cholesterol levels to normal limits. Materials and Methods: There were 35 Isa Brown laying hens aged 40 weeks with health conditions chosen as the object of research. We used a completely randomized design method with seven treatments. Treatments included P0: basal feed; T1: basal feed and probiotic liquid 2%; T2: basal feed and probiotic liquid 3%; T3: basal feed and probiotic liquid 4%; T4: basal feed and probiotic powder 2%; T5: basal feed and probiotic powder 3%; and T6: basal feed and probiotic powder 4%. On day 35 of the research, we collected blood samples. We analyzed the data using analysis of variance, followed by Duncan's multiple range test. Results: This showed that probiotic liquid and powdered yogurt had a significant effect (p < 0.05) on all parameters. Supplementation with 4% probiotic powder reduced neutrophil (53.96%), lymphocyte (27.84%), and N/L ratios (36.25%); alkaline phosphatase levels (53.6%); and cholesterol levels (ApB 26.65% and LDL 42.4%) compared to the control. Conclusion: This study shows that all probiotic supplementation shows improvement in the studied parameters, but the addition of 4% probiotic powder had the best result for reducing neutrophil, lymphocyte, N/L ratio, alkaline phosphatase, and cholesterol levels compared to the control and probiotic liquid. [J Adv Vet Anim Res 2024; 11(4.000): 936-943]

Objective: The efficiency of corona discharge (CD) for detoxification of aflatoxin B1 (AB1), ochra¬toxin A (OA), and fumonisin B1 (FMB1) from poultry feeds with its influences on feed components was investigated. Materials and Methods: Feed samples were exposed to CD for six durations (10, 20, 30, 40, 50, and 60 min) at three distances (1.5, 2.5, and 3.5 cm). Mycotoxin levels were estimated by compet¬itive enzyme-linked immunosorbent assay, and findings were substantiated by high-performance liquid chromatography. Results: AB1, OA, and FMB1 degradation percentages increased significantly (p < 0.05) with pro¬cessing times increment and distances reduction to reach values of 83.22%, 84.21%, and 84.76% at the first distance; 80.28%, 84.00%, and 84.12% at the second distance; and 68.30%, 71.74%, and 76.18% at the third distance, respectively, after 60 min of treatment. FMB1 reported the highest degradation level. Concerning CD impacts on feed composition, protein, fat, and moisture contents decreased significantly (p < 0.05). Carbohydrates and ash were not affected adversely. Depending on peroxide values estimation, fats were of good quality. Conclusion: The CD effectiveness for AB1, OA, and FMB1 detox from poultry feeds with moderate impact on the quality of feed. [J Adv Vet Anim Res 2024; 11(4.000): 819-834]

Objective: The present work aimed to determine the impact of various cooking methods on sensory attributes, microbial safety, and physicochemical characteristics of ostrich meat to specify the best cooking method that guarantees the microbial safety of the meat as well as maintains nutritional values and is highly attractive to consumers. Materials and Methods: One hundred fresh leg muscles of ostriches were divided into five groups. Different cooking techniques were used for each group, as follows: roasting, boiling, grilling, frying, and microwaving. Each method was examined by evaluating the impact of various cooking methods on sensory attributes, proximate chemical analysis, protein and fat oxidation parameters, microbial load, changes in color, and the shear force of ostrich meat. Results: The oven-roasting and grilling methods are highly recommended and more appealing to consumers since they produce tenderer and juicier meat, cause less cooking loss (CL), and maintain the nutritive value of ostrich meat; however, they have the highest protein and fat oxidation rates. On the other hand, boiling and frying methods revealed good fat oxidation parameters, the highest CL, and preserved nutritional value, but unfortunately, they were not highly preferred by consumers. From a hygienic point of view, grilling and microwave cooking are the methods that ensure the microbiological safety of cooked ostrich meat, as they significantly reduce Enterobacteriaceae and psychrotrophic bacterial counts, among other cooking methods. Conclusion: The oven roasting and grilling methods were the most preferable thermal cooking techniques, as they achieved the highest acceptability to consumers and maintained the nutritive values of ostrich meat. [J Adv Vet Anim Res 2024; 11(1.000): 194-202]