Tick-borne diseases (TBDs) or otherwise called equine piroplasmosis (EP) are the foremost economic limitations to equids production. Thus, reducing the breeding capability and athletic performance of equids globally. Identification of these haemoparasites is crucial in understanding their distribution in the population and it is imperative to discern between species and subspecies that are responsible for the occurrence of the disease conditions. Conventional procedures such as microscopic and serological evaluations do not usually meet these prerequisites. Diagnostic contrivances, such as the complement fixation test (CFT), the indirect fluorescent antibody test (IFAT) and the enzyme linked immunosorbent assay (ELISA) have been efficaciously used for many years. Furthermore, DNA-based investigations for identification, differentiation and classification of different haemoparasites have also been established. Molecular diagnostic procedures, such as DNA hybridization, polymerase chain reaction (PCR), transcriptomics, proteomics, metagenomics and metabolomics, permit the uncovering of parasites in blood, tissues or ticks with optimal sensitivity, specificity and consistency. In addition, these procedures can be exploited to detect definite species and subspecies. The prerequisite of these investigations must include proper premeditation and validation, these investigations provide an effective device for molecular studies, with greater benefits of flexibility to standardization. The application of these procedures for studying TBDs or EP globally will be irreplaceable for a long period from now. Therefore, the aim of this review is to draw up the specifics of the procedures in more convenient form for practitioners and researchers. KEY WORDS: Diagnosis, equids, molecular, transcriptomics, proteomics, metagenomics, metabolomics, haemoparasites, tick-borne diseases [J Adv Vet Anim Res 2016; 3(2.000): 84-91]

Objectives: This study was designed to reveal out the present transportation scenario of Black Bengal goats to and from some selected markets in Bangladesh. Materials and methods: A questionnaire survey to know the behavioral parameters and management provisions provided by the farmers was designed to collect necessary information from Sirajganj and Gabtoli markets. The survey was conducted by direct interview with the stakeholders who were directly involved with the transportation of Black Bengal goats from Sirajganj to Dhaka city. Results: The present study showed that 58% adult respondents were involved in goat transportation program, among them 95% were male. Most of the farmers in the markets lacked institutional education (24%) or just completed primary education (41%). Only 27% of the respondents had earlier experience in transporting animals. Transport durations were <6 h (51%), 6 h (39%), and >6 h (10%). The goats were usually transported on foot or by using vehicles. All the respondents were known about planning of animal transportation. About 2, 5, 7 and 5% of animals were suffering from head swinging, stretching, stamping of feet and paralyzed condition during transportation, respectively. During transportation, the animals exhibited idleness (25%), panting (17%), foaming (14%), vocalization (13%) and moving forward (12%). Most of the farmers provided feed (41%), rest (33%) and shelter (24%) during the transportation of the goats. Only 5% farmers hired trained drivers for transportation purpose. About 27% animals were untied and 7% animals ramped during transportation. Besides, there were high percentage of pressure to animals (23%), hitting or kicking animals (19%) and abuse to animals (15%) during transportation. Conclusion: From this study it is concluded that Black Bengal goats became physically unfit during long time transportation although majority of the farmers took proper management practices. Therefore, changes in behavioral responses might be alleviated through creating awareness to animal raiser as well transporter. [J Adv Vet Anim Res 2016; 3(1.000): 38-44]