The haemostatic capability, adhesion ability and biocompatibility of chitosan sponges was compared with conventional method as control group. The chitosan sponges were briefly immersed in an aqueous 20% ammonia solution before being applied to a rabbit dorsal nasal vein wound. The total amount of bleeding from the injured veins until hemostasis was similar for both chitosan and control group. The complete hemostasis success rates for both the chitosan and control group were also similar. Under hemostasis, the chitosan sponges strongly adhered to the surface of the rabbit muscles, whereas the control group. Under wet conditions, however, there was no significant difference in the adhesive ability between the two groups. During implantation, the chitosan sponges were much more flexible and resistant to breakage that good. The biocompatibilities in addition, biodegradation rates of the Chitosan sponges were very different after subcutaneous implantation in rabbit.

Destruction of spleen and kidney components in C. idella started on the 17th day in specimens subjected to 20 µg•L-1 Cd . The spleen was actually destructed after 73 days of exposure to 60 µg•L-1 Cd , where the two pulps can not be distinguished clearly, as the splenic blood vessels contained less blood cells . The kidney showed a complete destruction and the hematopoietic tissue lacked a distinguishable border whereas the necrosis covered large area after 60 days at 60 µg•L-1Cd . The degree of destruction was lineary proportional to concentration of toxicant and period of exposure although the period was somewhat more effective . It was concluded that the histological alterations in spleen and kidney could be used as an indicator for water pollution with cadmium and for fish health as well .

This present study was designed to investigate the effects of two levels of cadmium chloride (30 and 40 part per billion (ppb)) in drinking water on body weight, relative weight of testes and epididymus (head, body, tail), diameter and thickness of seminiferous tubules. Eighteen mature male rabbits were randomly divided into three equal groups; the control group (GI) were given ordinary tap water and animals in the first treated group (GII) were received 30 part per billion (ppb) of cadmium chloride in drinking water; while animals in the second treated group(GIII) were given 40 ppb of cadmium chloride in drinking water. Weight of animals were taken at the pre-treated period and every three weeks during the treated period. At the end of experiment the animals were killed, testis and epididymus were taken in order to measuring their weight. Samples testis were taken for histological study and for measuring the diameter of the seminiferous tubules and the thickness of the cells lining their. The results showed a significant increase (p

The present work making histological investigation of certain part of medulla oblongata on seven one humped camel ( Camelus dromedarius ) in different ages and sexes, the Trigeminal V, Abducent VI, Facial VII, and Vestibulocochlear VIII nerve nuclei in camel was noticed. These nuclei are in close resamble in other mammals in location and the cells ( neuron ) type found in these nuclei. This study help to present an information on these nuclei cytoarchitecture and thier location, and may answers of many question about this animal (Camel ) which walking across huge deserts.

The reaction of 3,5-dimethyl-1H-pyrazole with mercury (II)acetate in absolute methanol gave (3,5-dimethyl-1H-pyrazol-4-yl) mercury(II) chloride (DMPMC), which characterized by microanalysis, 1H, and 13C-NMR and IR. It was found that 0.22M of (DMPMC) dissolved in ethanol/water added to Muller-Hinton Agar medium and Sabourauds-Dextrose agar medium (SDA) respectively, inhibited the growth of some gram negative bacteria [Escherichia-coli, Klebsiella-aerogenes and Pseudomonas-aeruginosa], also gram positive bacteria [Staphylococcus-aureus, Streptococcus-pyogenes] and Candida-albicans as well as some fungi [Aspergillus-flavus, Aspergillus-fumigatus and Aspergillus-niger]. Higher concentrations of (DMPMC) solution in to the media inhibited growth of bacteria yeast and fungi under studies more strongly. The minimal inhibitory concentration (MIC)and the cytotoxicity of (DMPMC) were studied against human being blood , it was found that it has no haemolysis at different concentrations in vitro

It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesionduring infection. This paper reports the partial purification and characterization of the HA-L haemagglutininfrom H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that hasbeen shown to be the serovar specific haemagglutinin in this organism. Using the pI and molecularmass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 °C. It was also shown that activity is enhanced by the addition of Ca2+ and Mn2+ ions.

The present study investigated the distribution of nerves in the ovary of the emu. The neuronal markers, protein gene product 9.5, neurofilament protein and neuron specific enolase demonstrated the constituents of the extrinsic and intrinsic ovarian neural systems. The extrinsic neural system was composed of ganglia in the ovarian stalk, as well as nerve bundles, which were distributed throughout the ovary. Isolated neuronal cell bodies, in the medulla and cortex, formed the intrinsic neural system. An interesting finding of the study was the presence of nerve bundles, circumscribed by lymphocytes, in the ovarian stalk. The findings of the study indicate that the distribution of nerve fibres and neuronal cell bodies in the emu ovary is similar, but not identical to that of the domestic fowl and ostrich.

Experimental Trichinella zimbabwensis infections were established in three baboons (Papios p.)and four vervet monkeys (Cercopithecuase thiops) and the clinical-pathological manifestations assessed. The infected animals showed clinical signs ranging from fever, diarrhoea, periorbitaol edema and muscular pain in varying degrees. One baboon became blind due to the infection. Levels of creatinine phosphokinase and lactated ehydrogenase increased to reach a peak on Day 42 post-infection(pi)for both baboons and monkeys. Blood parameters such as packed cell volume, levels of red blood cells and white blood cells did not change significantly from the normal ranges except for the levels of eosinophils which peaked above the normal ranges at Day 28 and 56 pi in baboons and at Day 56 pi in monkeys.

The prevalence and geographic distribution of the fowl tampan, Argas walkerae Kaiser & Hoogstraal, 1969 was determined in the eastern region of the Eastern Cape Province, South Africa by inspecting two fowl houses in the vicinity of each of 72 randomly selected communal cattle dip-tanks. Tampans were collected from 102 (70.8 %) of the 144 fowl houses in the neighbourhood of 57 (79.2 %) of the 72 selected dip-tanks, and the localities of the collections were mapped. Argas walkerae was present in fowl houses from the warm coastal regions of the Indian Ocean in the south to the cold and mountainous Drakensberg in the north-east of the Province. Taking into account the probable sensitivity of the sampling method, it is estimated that A. walkerae is likely to be present in fowl houses belonging to between 74 and 84 % of communities making use of cattle dip-tanks in the eastern region of the Eastern Cape Province, and that when it is present, between 64 and 75 % of fowl houses will be infested. The geographic distribution of A. walkerae seemed to be more strongly associated with the presence of fowls and fowl houses containing raw or processed wood in their structure than with climate.

Spirocerca lupi (Spirurida: Spirocercidae) is a cosmopolitan parasite, principally of domestic dogs and dung beetles are its main intermediate hosts. In South Africa there has recently been growing concern over the upsurge of reported cases of clinical spirocercosis in dogs, while little is known or understood about the dynamics of the host-parasite associations between dung beetles and this nematode. We determined and compared the prevalence of infection in dung beetles between rural, urban and periurban areas of Tshwane (Pretoria) Metropole. Dung beetles were sampled during April and October 2006, at various localities in each of these areas. Localities were selected on the basis of being focal areas of high infection with S. lupi in dogs. Pig, dog and cow dung-baited pitfall traps were used for sampling the beetles. Trap contents were collected 48 h after the traps had been set and only dung beetles were collected from the traps. In total, 453 specimens belonging to 18 species were collected from 63 pitfall traps in all three areas. The numbers of species that were collected varied among the three areas. Dung beetles, irrespective of species (18) and numbers (447), predominantly preferred pig dung. The prevalence of dung beetles infected with the larvae of S. lupi varied considerably in the three areas. In the urban area 13.5 % of the dung beetles dissected were infected, while the prevalence of S. lupi in dung beetles in the rural area was 2.3 %. All the dung beetles that were infected with this nematode showed a preference for omnivore (pig and dog) dung.