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النتائج 71 - 80 من 535
Thoracic limb morphology of the red panda (Ailurus fulgens) evidenced by osteology and radiography النص الكامل
2015
Makungu, Modesta(University of Pretoria Department of Anatomy and Physiology ,Sokoine University of Agriculture Department of Veterinary Surgery and Theriogenology) | Groenewald, Hermanus B.(University of Pretoria Department of Anatomy and Physiology) | du Plessis, Wencke M.(Ross University School of Veterinary Medicine) | Barrows, Michelle(Bristol Zoo Gardens) | Koeppel, Katja N.(Johannesburg Zoo)
Thoracic limb morphology of the red panda (Ailurus fulgens) evidenced by osteology and radiography النص الكامل
2015
Makungu, Modesta(University of Pretoria Department of Anatomy and Physiology ,Sokoine University of Agriculture Department of Veterinary Surgery and Theriogenology) | Groenewald, Hermanus B.(University of Pretoria Department of Anatomy and Physiology) | du Plessis, Wencke M.(Ross University School of Veterinary Medicine) | Barrows, Michelle(Bristol Zoo Gardens) | Koeppel, Katja N.(Johannesburg Zoo)
The red panda (Ailurus fulgens) is distributed primarily in the Himalayas and southern China. It is classified as a vulnerable species by the International Union for Conservation of Nature. The aim of this study was to describe the normal osteology and radiographic anatomy of the thoracic limb of the red panda. Radiography of the right thoracic limb was performed in seven captive adult red pandas. Radiographic findings were correlated with bone specimens from three adult animals. The scapula was wide craniocaudally and presented with a large area for the origin of the teres major muscle. The square-shaped major tubercle did not extend proximal to the head of the humerus. The medial epicondyle was prominent. A supracondylar foramen was present. The radial tuberosity and sesamoid bone for the abductor digiti I longus were prominent. The accessory carpal bone was directed palmarolaterally. Metacarpal bones were widely spread. The thoracic limb morphology of the red panda evidenced by osteology and radiography indicated flexibility of the thoracic limb joints and well-developed flexor and supinator muscles, which are important in arboreal quadrupedal locomotion. Knowledge gained during this study may prove useful in identifying skeletal material or remains and diagnosing musculoskeletal diseases and injuries of the thoracic limb.
اظهر المزيد [+] اقل [-]The slaughter of increased numbers of pregnant cows in Tanga abattoir, Tanzania: A cause for concern? النص الكامل
2015
Swai, Emmanuel S.(Ministry of Livestock Development and Fisheries) | Hayghaimo, Abdu A.(Ministry of Livestock Development and Fisheries) | Hassan, Ayubu A.(Tanga Municipal Livestock Office) | Mhina, Bartholomeo S.(Tanga Municipal Livestock Office)
The slaughter of increased numbers of pregnant cows in Tanga abattoir, Tanzania: A cause for concern? النص الكامل
2015
Swai, Emmanuel S.(Ministry of Livestock Development and Fisheries) | Hayghaimo, Abdu A.(Ministry of Livestock Development and Fisheries) | Hassan, Ayubu A.(Tanga Municipal Livestock Office) | Mhina, Bartholomeo S.(Tanga Municipal Livestock Office)
Information on the level of foetal wastage in slaughtered cattle in Tanzania is limited. A three-month observational study (April - June 2014) of animals slaughtered at the Tanga abattoir in Tanga region, Tanzania was carried out to determine the number of pregnant cows slaughtered. The total number of cattle slaughtered during the study period was 3643, representing a monthly kill average of 1214 and a daily kill average of 40. Over 98% of the cattle presented to the abattoir for slaughter were local breed (Tanzania shorthorn zebu) and most were above 3 years of age. Improved breeds of cattle represented only 1.3% of all slaughters. Of the cattle slaughtered, 2256 (61.9%) were female and 1387 (38.1%) were male. A total of 655 slaughtered cows were pregnant, representing a foetal wastage of 29.1%. Of the 655 recovered foetuses, 333 (50.8%) were male and 322 (49.2%) were female. Of the recovered foetuses, 25.8% were recovered in the first, 42.7% in the second and 31.6% in the third trimester. This study indicates cases of significant foetal losses, negatively impacting future replacement stock as a result of the slaughter of pregnant animals. The indiscriminate slaughter of pregnant cows suggests that existing animal welfare legislation is not sufficiently enforced and routine veterinary ante-mortem inspection of trade animals is failing to prevent the high level of foetal wastage.
اظهر المزيد [+] اقل [-]The slaughter of increased numbers of pregnant cows in Tanga abattoir, Tanzania: A cause for concern? النص الكامل
2015
Emmanuel S. Swai | Abdu A. Hayghaimo | Ayubu A. Hassan | Bartholomeo S. Mhina
Information on the level of foetal wastage in slaughtered cattle in Tanzania is limited. A three-month observational study (April – June 2014) of animals slaughtered at the Tanga abattoir in Tanga region, Tanzania was carried out to determine the number of pregnant cows slaughtered. The total number of cattle slaughtered during the study period was 3643, representing a monthly kill average of 1214 and a daily kill average of 40. Over 98% of the cattle presented to the abattoir for slaughter were local breed (Tanzania shorthorn zebu) and most were above 3 years of age. Improved breeds of cattle represented only 1.3% of all slaughters. Of the cattle slaughtered, 2256 (61.9%) were female and 1387 (38.1%) were male. A total of 655 slaughtered cows were pregnant, representing a foetal wastage of 29.1%. Of the 655 recovered foetuses, 333 (50.8%) were male and 322 (49.2%) were female. Of the recovered foetuses, 25.8% were recovered in the first, 42.7% in the second and 31.6% in the third trimester. This study indicates cases of significant foetal losses, negatively impacting future replacement stock as a result of the slaughter of pregnant animals. The indiscriminate slaughter of pregnant cows suggests that existing animal welfare legislation is not sufficiently enforced and routine veterinary ante-mortem inspection of trade animals is failing to prevent the high level of foetal wastage.
اظهر المزيد [+] اقل [-]Determination of oxytetracycline residues in cattle meat marketed in the Kilosa district, Tanzania النص الكامل
2015
Kimera, Zuhura I.(Ministry of Livestock and Fisheries Development Department of Fisheries Development) | Mdegela, Robinson H.(Sokoine University of Agriculture Department of Veterinary Medicine and Public Health) | Mhaiki, Consolatha J.N.(Sokoine University of Agriculture Department of Soil Science) | Karimuribo, Esron D.(Sokoine University of Agriculture Department of Veterinary Medicine and Public Health) | Mabiki, Faith(Sokoine University of Agriculture Department of Physical Science) | Nonga, Hezron E.(Sokoine University of Agriculture Department of Veterinary Medicine and Public Health) | Mwesongo, James(Sokoine University of Agriculture Department of Physical Science)
Determination of oxytetracycline residues in cattle meat marketed in the Kilosa district, Tanzania النص الكامل
2015
Kimera, Zuhura I.(Ministry of Livestock and Fisheries Development Department of Fisheries Development) | Mdegela, Robinson H.(Sokoine University of Agriculture Department of Veterinary Medicine and Public Health) | Mhaiki, Consolatha J.N.(Sokoine University of Agriculture Department of Soil Science) | Karimuribo, Esron D.(Sokoine University of Agriculture Department of Veterinary Medicine and Public Health) | Mabiki, Faith(Sokoine University of Agriculture Department of Physical Science) | Nonga, Hezron E.(Sokoine University of Agriculture Department of Veterinary Medicine and Public Health) | Mwesongo, James(Sokoine University of Agriculture Department of Physical Science)
Oxytetracycline is used to treat various diseases in cattle. However, its use may be associated with unacceptable residue levels in food. Oxytetracycline residues in tissues from indigenous cattle were determined in a cross-sectional study conducted in the Kilosa district, Tanzania, between November 2012 and April 2013. A total of 60 tissue samples, including muscle, liver and kidney, were collected from slaughterhouses and butchers and analysed for oxytetracycline using high-performance liquid chromatography. Oxytetracycline residues were found in 71.1% of the samples, of which 68.3% were above acceptable regulatory levels. The mean concentration of oxytetracycline across tissues was 3401.1 μg/kg ± 879.3 μg/kg; concentrations in muscle, liver and kidney were 2604.1 μg/kg ± 703.7 μg/kg, 3434.4 μg/kg ± 606.4 μg/kg and 3533.1 μg/kg ± 803.6 μg/kg, respectively. High levels of oxytetracycline residue in meat from indigenous cattle may pose a health threat to consumers in Kilosa. The findings possibly reflect a general lack of implementation of recommended withdrawal periods, ignorance about drug use and lack of extension services. Strict regulation of the use of antimicrobial drugs in the livestock industry and associated testing of animal-derived food sources prior to marketing are required.
اظهر المزيد [+] اقل [-]Determination of oxytetracycline residues in cattle meat marketed in the Kilosa district, Tanzania النص الكامل
2015
Zuhura I. Kimera | Robinson H. Mdegela | Consolatha J.N. Mhaiki | Esron D. Karimuribo | Faith Mabiki | Hezron E. Nonga | James Mwesongo
Oxytetracycline is used to treat various diseases in cattle. However, its use may be associated with unacceptable residue levels in food. Oxytetracycline residues in tissues from indigenous cattle were determined in a cross-sectional study conducted in the Kilosa district, Tanzania, between November 2012 and April 2013. A total of 60 tissue samples, including muscle, liver and kidney, were collected from slaughterhouses and butchers and analysed for oxytetracycline using high-performance liquid chromatography. Oxytetracycline residues were found in 71.1% of the samples, of which 68.3% were above acceptable regulatory levels. The mean concentration of oxytetracycline across tissues was 3401.1 μg/kg ± 879.3 μg/kg; concentrations in muscle, liver and kidney were 2604.1 μg/kg ± 703.7 μg/kg, 3434.4 μg/kg ± 606.4 μg/kg and 3533.1 μg/kg ± 803.6 μg/kg, respectively. High levels of oxytetracycline residue in meat from indigenous cattle may pose a health threat to consumers in Kilosa. The findings possibly reflect a general lack of implementation of recommended withdrawal periods, ignorance about drug use and lack of extension services. Strict regulation of the use of antimicrobial drugs in the livestock industry and associated testing of animal-derived food sources prior to marketing are required.
اظهر المزيد [+] اقل [-]Peste des petits ruminants outbreaks in White Nile State, Sudan النص الكامل
2015
Ishag, Osama M.(Rabak Veterinary Research Laboratory) | Saeed, Intisar K.(Veterinary Research Institute Department of Virology) | Ali, Yahia H.(Veterinary Research Institute Department of Virology)
Peste des petits ruminants outbreaks in White Nile State, Sudan النص الكامل
2015
Ishag, Osama M.(Rabak Veterinary Research Laboratory) | Saeed, Intisar K.(Veterinary Research Institute Department of Virology) | Ali, Yahia H.(Veterinary Research Institute Department of Virology)
Eight outbreaks of peste des petits ruminants in sheep and goats were reported in White Nile State, Sudan, between 2008 and 2009. A mortality rate of 4.2% was reported across the different outbreaks. Clinically the disease was characterised by high fever, ocular and nasal discharge, pneumonia, ulceration of the mucous membranes, diarrhoea and death. The postmortem findings included necrotic lesions in the mouth and gastrointestinal tract, and swollen, oedematous lymph nodes associated with the lungs and intestine. Of the 209 serum samples tested by competitive enzyme-linked immunosorbent assay, 113 (54%) were found positive. Peste des petits ruminants virus was confirmed in tissues, nasal swabs and blood samples by immunocapture enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction and isolation of the virus in culture of lamb testicle cells.
اظهر المزيد [+] اقل [-]Peste des petits ruminants outbreaks in White Nile State, Sudan النص الكامل
2015
Osama M. Ishag | Intisar K. Saeed | Yahia H. Ali
Eight outbreaks of peste des petits ruminants in sheep and goats were reported in White Nile State, Sudan, between 2008 and 2009. A mortality rate of 4.2% was reported across the different outbreaks. Clinically the disease was characterised by high fever, ocular and nasal discharge, pneumonia, ulceration of the mucous membranes, diarrhoea and death. The postmortem findings included necrotic lesions in the mouth and gastrointestinal tract, and swollen, oedematous lymph nodes associated with the lungs and intestine. Of the 209 serum samples tested by competitive enzyme-linked immunosorbent assay, 113 (54%) were found positive. Peste des petits ruminants virus was confirmed in tissues, nasal swabs and blood samples by immunocapture enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction and isolation of the virus in culture of lamb testicle cells.
اظهر المزيد [+] اقل [-]Equine encephalosis in Thoroughbred foals on a South African stud farm النص الكامل
2015
Grewar, John D.(Western Cape Department of Agriculture ,University of Pretoria Department of Production Animal Studies) | Thompson, Peter N.(University of Pretoria Department of Production Animal Studies) | Lourens, Carina W.(University of Pretoria Equine Research Centre) | Guthrie, Alan J.(University of Pretoria Equine Research Centre)
Equine encephalosis in Thoroughbred foals on a South African stud farm النص الكامل
2015
Grewar, John D.(Western Cape Department of Agriculture ,University of Pretoria Department of Production Animal Studies) | Thompson, Peter N.(University of Pretoria Department of Production Animal Studies) | Lourens, Carina W.(University of Pretoria Equine Research Centre) | Guthrie, Alan J.(University of Pretoria Equine Research Centre)
Thoroughbred foal body temperature data were collected from shortly after birth until shortly after weaning during the 2007/2008 season on a stud farm in the Western Cape Province of South Africa. Equine encephalosis (EE) caused by EE virus (EEV) serotype 4 (EEV-4) occurred in the foal group during the first autumn after their birth (March and April 2008). A descriptive study was undertaken to provide data on the EEV maternal antibody status, the association between pyrexia and EEV infection, and the incidence of infection amongst the foals prior to and during the episode. This included the frequent capturing of foal body temperature data and regular collection of serum and whole blood during pyretic episodes. Infection by EEV was determined using both virological and serological methods. A high EE incidence of at least 94% occurred amongst the foal cohort, despite the fact that 37% of foals had previously shown maternal antibody to EEV-4. Pyrexia in foals was not directly associated with EE infection and 41% of infected foals showed no detectable pyretic episode. Information obtained from this EE episode showed the high incidence of EEV infection in foals during the first autumn after their birth. Monitoring foal body temperature can alert farmers to outbreaks of infectious disease, such as EE. These results are relevant to the epidemiology of EE and facilitate greater understanding of it as a differential diagnosis of African horse sickness (AHS), given that EE and AHS have similar epidemiologic profiles.
اظهر المزيد [+] اقل [-]Equine encephalosis in Thoroughbred foals on a South African stud farm النص الكامل
2015
John D. Grewar | Peter N. Thompson | Carina W. Lourens | Alan J. Guthrie
Thoroughbred foal body temperature data were collected from shortly after birth until shortly after weaning during the 2007/2008 season on a stud farm in the Western Cape Province of South Africa. Equine encephalosis (EE) caused by EE virus (EEV) serotype 4 (EEV-4) occurred in the foal group during the first autumn after their birth (March and April 2008). A descriptive study was undertaken to provide data on the EEV maternal antibody status, the association between pyrexia and EEV infection, and the incidence of infection amongst the foals prior to and during the episode. This included the frequent capturing of foal body temperature data and regular collection of serum and whole blood during pyretic episodes. Infection by EEV was determined using both virological and serological methods. A high EE incidence of at least 94% occurred amongst the foal cohort, despite the fact that 37% of foals had previously shown maternal antibody to EEV-4. Pyrexia in foals was not directly associated with EE infection and 41% of infected foals showed no detectable pyretic episode. Information obtained from this EE episode showed the high incidence of EEV infection in foals during the first autumn after their birth. Monitoring foal body temperature can alert farmers to outbreaks of infectious disease, such as EE. These results are relevant to the epidemiology of EE and facilitate greater understanding of it as a differential diagnosis of African horse sickness (AHS), given that EE and AHS have similar epidemiologic profiles.
اظهر المزيد [+] اقل [-]Renal, gastrointestinal, and hemostatic effects of oral administration of meloxicam to Hispaniolan Amazon parrots (Amazona ventralis) النص الكامل
2015
Dijkstra, Bas | Sanchez-Migallon Guzman, David | Gustavsen, Kate | Owens, Sean D. | Hass, Carlyle | Kass, Philip H. | Paul-Murphy, Joanne R.
OBJECTIVE To investigate renal, gastrointestinal, and hemostatic effects associated with oral administration of multiple doses of meloxicam to healthy Hispaniolan Amazon parrots (Amazona ventralis). ANIMALS 12 Hispaniolan Amazon parrots. PROCEDURES Birds were assigned to receive meloxicam oral suspension (1.6 mg/kg, PO, q 12 h) and 2.5 mL of tap water inserted into the crop by use of a gavage tube (n = 8) or the equivalent volume of tap water only (control group; 4) for 15 days. Urine and feces were collected 2 hours after treatment administration each day. Feces were evaluated for occult blood. Results of a CBC and serum biochemical analysis and measured N-acetyl-β-d-glucosaminidase (NAG) activity and whole blood clotting time were evaluated before, during, and after completion of treatments. Results of urinalysis and measured urine NAG activity were also evaluated. RESULTS Birds treated with meloxicam had a significant increase in number of WBCs and decrease in PCV from before to after treatment. The PCV also decreased significantly, compared with results for the control group; however, WBC count and PCV for all birds remained within reference ranges throughout the study. One parrot treated with meloxicam had a single high value for urine NAG activity. CONCLUSIONS AND CLINICAL RELEVANCE Meloxicam administered orally at the dosage used in this study caused no apparent negative changes in several renal, gastrointestinal, or hemostatic variables in healthy Hispaniolan Amazon parrots. Additional studies to evaluate adverse effects of NSAIDs in birds will be needed.
اظهر المزيد [+] اقل [-]Effects of azathioprine, 6-mercaptopurine, and 6-thioguanine on canine primary hepatocytes النص الكامل
2015
LaDuke, Kathleen E. | Ehling, Sarah | Cullen, J. M. (John M.) | Bäumer, Wolfgang
OBJECTIVE To investigate the cytotoxic effects of azathioprine, 6-mercaptopurine, and 6-thioguanine on canine hepatocytes. SAMPLE Commercially available cryopreserved canine primary hepatocytes. PROCEDURES The study consisted of 2 trials. In trial 1, hepatocytes were incubated with azathioprine, 6-mercaptopurine, or 6-thioguanine at 1 of 6 concentrations (0.468, 0.937, 1.875, 3.750, 7.500, or 15.000 μmol/L) for 24, 48, or 72 hours. At each time, cell viability and lactate dehydrogenase (LDH) activity were determined for each thiopurine-concentration combination, and alanine aminotransferase (ALT) activity was determined for cells incubated with each thiopurine at a concentration of 15 μmol/L. In trial 2, hepatocytes were incubated with azathioprine, 6-mercaptopurine, or 6-thioguanine at 1 of 3 concentrations (18.75, 37.50, or 75.00 μmol/L) for 24 hours, after which the free glutathione concentration was determined for each thiopurine-concentration combination and compared with that for hepatocytes incubated without a thiopurine (control). RESULTS Incubation of hepatocytes with each of the 3 thiopurines adversely affected cell viability in a time- and concentration-dependent manner; however, this decrease in cell viability was not accompanied by a concurrent increase in LDH or ALT activity. Likewise, free glutathione concentration for hepatocytes incubated for 24 hours with supratherapeutic thiopurine concentrations (> 18.75 μmol/L) did not differ significantly from that of control cells.CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that thiopurines adversely affected the viability of canine hepatocytes in a time- and concentration-dependent manner but had a nonsignificant effect on the LDH and ALT activities and free glutathione depletion of those hepatocytes.
اظهر المزيد [+] اقل [-]Pharmacokinetics and pharmacodynamics of the factor Xa inhibitor apixaban after oral and intravenous administration to cats النص الكامل
2015
Myers, Jennifer A. | Wittenburg, Luke A. | Olver, Christine S. | Martinez, Caitlyn M. | Bright, Janice M.
OBJECTIVE To determine pharmacokinetic and pharmacodynamic properties of the novel factor Xa inhibitor apixaban in clinically normal cats. ANIMALS 5 purpose-bred domestic shorthair cats. PROCEDURES A single dose of apixaban (0.2 mg/kg, PO) was administered to each cat (time 0), and blood samples were obtained at 0, 15, 30, 45, 60, 120, 240, 360, 480, and 1,440 minutes. After a 1-week washout period, another dose of apixaban (0.2 mg/kg, IV) was administered to each cat, and blood samples were obtained at 0, 5, 10, 15, 30, 45, 60, 120, 240, 360, 480, and 1,440 minutes. Apixaban concentrations in plasma were measured via liquid chromatography–tandem mass spectrometry. Pharmacodynamic effects of apixaban were determined with a commercial assay for factor × activity, which measures endogenous factor Xa activity chromogenically. RESULTS Factor Xa was inhibited as a function of time after a single dose of apixaban administered orally or IV, and a direct inverse correlation with the plasma apixaban concentration was detected. Pharmacokinetic analysis revealed moderate clearance, short half-life, and high bioavailability for apixaban. A 2-compartment model was fit to the IV pharmacokinetic data; compartmental modeling could not be used to adequately describe the oral data because of substantial interindividual variability. CONCLUSIONS AND CLINICAL RELEVANCE Results inticated that apixaban was an effective inhibitor of factor Xa in cats. Further studies will be needed to determine pharmacokinetics and pharmacodynamics after multidose administration, effects of cardiac disease on pharmacokinetics and pharmacodynamics, dosing recommendations, and efficacy of apixaban for use in the treatment and prevention of thromboembolic disease in cats.
اظهر المزيد [+] اقل [-]In vitro mechanical evaluation of equine laryngeal tie-forward constructs prepared with different suture materials and placement patterns النص الكامل
2015
Santos, Marcos P. | Gutierrez-Nibeyro, Santiago D. | Horn, Gavin P. | Hicke, Joshua D. | Stewart, Matthew C. | Schaeffer, David J.
OBJECTIVE To compare the mechanical properties of laryngeal tie-forward (LTF) constructs prepared with different suture materials and suture placement patterns during single load to failure testing. SAMPLE Larynges harvested from 50 horse cadavers and 5 intact horse cadavers. PROCEDURES In vitro LTF constructs were created by a standard technique with polyester sutures, a standard technique with polyethylene sutures, a modified technique with metallic implants and polyester sutures, a modified technique with metallic implants and polyethylene sutures, or a modified tie-off technique with polyester sutures (10 of each type of construct). Mechanical properties including maximal load (N) at failure and failure mode were compared among constructs. Also, maximal loads at failure of the in vitro LTF constructs were compared with the loads exerted on the sutures tightened to achieve rostral laryngeal advancement in intact cadavers. RESULTS Constructs prepared by a standard technique with polyethylene sutures had a significantly higher pull out strength than those prepared by a modified technique with metallic implants and either polyester or polyethylene sutures. For constructs prepared by a standard technique with polyethylene sutures or similarly placed polyester sutures, maximal load at failure did not differ but the failure mode did differ significantly. The load to failure for all in vitro constructs was higher than the maximal load measured during a range of motion test in intact horse cadavers. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that LTF procedures can be performed in live horses with any of the suture materials and techniques tested.
اظهر المزيد [+] اقل [-]Comparison of the effects of glycerol, dimethyl sulfoxide, and hydroxyethyl starch solutions for cryopreservation of avian red blood cells النص الكامل
2015
Graham, Jennifer E. | Meola, Dawn M. | Kini, Nisha R. | Hoffman, Andrew M.
OBJECTIVE To compare effectiveness of glycerol, dimethyl sulfoxide (DMSO), and hydroxyethyl starch (HES) solutions for cryopreservation of avian RBCs. SAMPLE RBCs from 12 healthy Ameraucana hens (Gallus gallus domesticus). PROCEDURES RBCs were stored in 20% (wt/vol) glycerol, 10% (wt/vol) DMSO freezing medium, or various concentrations of HES solution (7.5%, 11.5%, and 20% [wt/vol]) and frozen for 2 months in liquid nitrogen. Cells were then thawed and evaluated by use of cell recovery and saline stability tests, cell staining (7-aminoactinomycin D and annexin V) and flow cytometry, and scanning electron microscopy. RESULTS Percentage of RBCs recovered was highest for 20% glycerol solution (mean ± SE, 99.71 ± 0.04%) and did not differ significantly from the value for 7.5% HES solution (99.57 ± 0.04%). Mean saline stability of RBCs was highest for 10% DMSO (96.11 ± 0.25%) and did not differ significantly from the value for 20% HES solution (95.74 ± 0.25%). Percentages of cells with 7-aminoactinomycin D staining but without annexin V staining (indicating necrosis or late apoptosis) were lowest for 10% DMSO freezing medium (3%) and 20% glycerol solution (1%) and highest for all HES concentrations (60% to 80%). Scanning electron microscopy revealed severe membrane changes in RBCs cryopreserved in 20% HES solution, compared with membrane appearance in freshly harvested RBCs and RBCs cryopreserved in 10% DMSO freezing medium. CONCLUSIONS AND CLINICAL RELEVANCE Cryopreservation of avian RBCs with HES solution, regardless of HES concentration, resulted in greater degrees of apoptosis and cell death than did cryopreservation with other media. Transfusion with RBCs cryopreserved in HES solution may result in posttransfusion hemolysis in birds.
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