Objective: The main aim of this case report is to present a case of feline hemotropic mycoplasmosis that occurred concurrently with bacterial cystitis following the bite. Material and methods: A 3-year-old intact male domestic shorthair cat weighing 3.7 kg was referred to the Universiti Malaysia Kelantan Veterinary Clinic with clinical signs of hematuria and dysuria. History revealed that it was managed outdoor, fed with kibbles and wet food, but with no vaccination and deworming. Upon physical examination, the cat had a dull appearance, pale mucous membrane, normal respiratory rate, hypothermia, and bradycardia. Upon the examination of the urogenital system, there were urine burns at the anal region, necrotized penile tip, and presence of bite wound observed at the perineal region. Turgid and enlarged urinary bladder was identified upon palpation. Results: Diagnostic investigation revealed the hemotropic mycoplasmosis via microscopy, while urine culture was positive for Escherichia coli infection. The cat was successfully treated symptomatically. Conclusion: However, the prognosis of this cat was guarded given that the anemia was unresolved at the point of discharge. [J Adv Vet Anim Res 2018; 5(4.000): 490-495]

Objective: The objective of this study was to assess the effects of anesthetic drugs on heart rate (HR) and heart rate variability (HRV) in dogs. Materials and Methods: Twelve healthy client-owned dogs of various breeds, including five females and seven males were used for elective surgery in this study. The dogs were pre-medicated with four protocols; (1) alfaxalone [at 3 mg/kg body weight (bwt)], (2) zolazepam + tiletamine (Zoletil) (at 5 mg/kg bwt), (3) diazepam (at 0.3 mg/kg bwt) + ketamine (at 5 mg/kg bwt), and (4) diazepam (at 0.3 mg/kg bwt) + propofol (at 5 mg/kg bwt). The HR and HRV of 12 dogs were recorded 20 min before and after the administration of the anesthetic drugs. Doppler was used to obtain systolic, diastolic, and mean blood pressures. Results: After anesthetic drug administration, the dogs pre-medicated and inducted with alfaxalone had the lowest HR values as compared with those of other protocols. The HRV low frequency and high frequency power ratio decreased in the dogs pre-medicated and intubated with alfaxalone. Conclusion: This study demonstrates that alfaxalone preserves the cardiovascular function; and hence, is considered as safe to use for the surgical applicability in dogs. [J Adv Vet Anim Res 2018; 5(4.000): 485-489]

Objective: The present study was carried out for the isolation, identification and antibiogram study of Salmonella spp. from apparently healthy Japanese quails (Coturnix japonica) at three different quail farms in Mymensingh, Bangladesh. Materials and methods: A total of 75 cloacal swab samples were randomly collected from apparently healthy Japanese quails from three different farms at Mymensingh, Bangladesh. The samples were subjected to a series of cultural and biochemical examination for the isolation of Salmonella followed by molecular detection by polymerase chain reaction (PCR). Motility of the Salmonella was performed by motility test and amplification of speF gene. The antibiogram profile of the isolates was also evaluated against commonly used antimicrobials by disc diffusion method. Results: The overall prevalence of Salmonella spp. in quails was found to be 13.33%(n=10/75). Out of the 10 isolates, seven were found to be motile. Farmwise, the prevalence of Salmonella spp. were 10%(n=3/30), 24%(n=6/25) and 5%(n=1/20) at the quail farms of Bangladesh Agricultural University (BAU), Shikarikanda and Akua, respectively. The antibiogram study revealed that all the isolates were resistant to both Erythromycin and Tetracycline. On the other hand, 100% isolates were sensitive to both Ciprofloxacin and Imipenem. Ninety percent isolates of Salmonella were resistant to Colistin sulphate. Neomycin was found to be sensitive to 80% Salmonella isolates. All the Salmonella isolates were found to be multidrug resistant (MDR). Conclusion: The presence of MDR Salmonella spp. in quails signifies public health importance of the organisms, which may be associated with food-borne illness. [J Adv Vet Anim Res 2018; 5(1.000): 60-66]

Objective: This study was carried out to evaluate the effect of garlic (Allium sativum) with its immunomodulatory activity, on the susceptibility of broilers to infectious bursal disease, being an immunosuppressive disease. Materials and methods: Day-old broilers (102) were separated into 6 groups A-F of 17 each. Groups A, B and C had 0.125% of garlic-meal in feed. At 8 and 18 days of age groups A, C, D and F were administered IBD vaccine and groups B, C, D and E were infected with 1LD50 IBD virus (10-3.4 in 0.08ml PBS) via conjuctival instillation at 4 week-old. Clinical signs, mortality and gross pathological lesions were scored. Histopathological lesions in bursae of Fabricius were recorded. Virus antibody titre in serum was assayed at 1 day-old, 4 and 6 weeks-old using quantitative agar gel immunodiffusion test. Data generated was analysed using descriptive statistics, ANOVA and Duncans multiple comparison tests (P [J Adv Vet Anim Res 2018; 5(3.000): 275-281]

Objective: The scope of anesthesiology is extended immensely. The aim of this study was to know the farmers understanding and scope of veterinary anesthesiology. Materials and method: A total of 155 data were collected from different Upazilla Livestock Office and Veterinary Hospitals during the period from July to December 2017 through interview with a pretested questionnaire. Farmers age, sex, educational status and occupation were considered during interview. Results: We found that 27.74% farmer had the experienced of their animals surgery whereas only 20% knew that anesthesia is given before surgery which is significantly (p [J Adv Vet Anim Res 2018; 5(3.000): 369-373]

Objective: The study was intended to design the new specific primer targeting on mitochondrial D-Loop gene (D-Loop 443 primer) combined with a real-time polymerase chain reaction (PCR) for the analysis of wild boar meat (WBM) in food products of meatball. Materials and methods: The primer was designed and subjected to primer-basic local alignment search tool using National Center for Biotechnology Information software. Validation of real-time PCR using designed primer was performed by evaluation of several performance characteristics which included specificity, sensitivity, repeatability, linearity, and efficiency. Results: The results showed that the D-loop primer could be attached at 60.7°C and no amplification was detected against other species confirming the specificity of the primers. The limits of detection were found to be 4.68 ng and 2.34 ng using DNA extracted from WBM and that extracted from wild boar in meatball product. The D-Loop 443 primer was successfully used for the analysis of commercial meatball samples. Conclusion: The developed method can be proposed as a standard method for the identification of WBM in meatball to support halal products authentication. [J Adv Vet Anim Res 2018; 5(3.000): 361-368]

Objective: Sarcocystis spp. are common parasites and in terms of economics and pathogenicity in domestic animals is important. The purpose of this work was to define the rate of contamination of slaughtered carcasses of cattle to Sarcocystis using digestive and histopathological methods in southeast Iran. Material and Methods: In this descriptive laboratory study for 1 year, 500 carcasses were examined and isolated bradyzoites of Sarcocystis with the digest method. Also, tissue samples from the esophagus and diaphragm were considered for pathologic studies and stained with hematoxylin and eosin of sections of histopathological. Results: The results showed that the highest contaminations were in imported male animals aged 23 years old in the spring. There was a significant difference (p < 0.05) in the prevalence rate with the sex and race of cattle but no significant difference (p > 0.05) in the prevalence rate with age and season. Conclusion: Infection with Sarcocystis is common in oxen in this region. The imported cattle are more infected. It seems that racing and the environmental condition affect the prevalence of Sarcocystosis. [J Adv Vet Anim Res 2018; 5(4.000): 381-387]

Objective: This current study was subjected to investigate the influence of two-stage fermentation by Chrysonilia crassa and Bacillus subtilis on nutritional values and antioxidative properties of agro-industrial by-products. Materials and methods: Two-stage fermentation with Ch. crassa (inoculated in advance; single-step fermentation) and B. subtilis (inoculated later; two-step fermentation) was conducted on agro-industrial by-products, i.e., banana peel meals, cassava pulp, and rice bran. The pH measurement, microbial enumeration, proximate, and antioxidant analyses were conducted following 4- and 2-days aerobic incubation with Ch. crassa and B. subtilis, respectively. Results: The pH of banana peels and cassava pulp increased with Ch. crassa-fermentation, but then decreased following B. subtilis-fermentation. Chrysonilia crassa-fermentation did not change, but B. subtilis-fermentation decreased pH of rice bran. The number of lactic acid bacteria was higher in two-stage than in single-stage fermented by-products. Crude protein and fat were higher in fermented than in unfermented banana peels. Crude protein was higher in single- and two-stage fermented, while fat higher in single-stage fermented than in unfermented cassava pulp. Crude fat and ash contents increased with fermentation in rice bran. Single-stage fermentation increased some of amino acids contents in banana peels and cassava pulp. The concentration of polyphenols, tannins, and antioxidant potential of banana peels reduced with fermentation. Total polyphenols and tannins were higher, whereas antioxidant activity was lower in fermented than in unfermented cassava pulp. Total polyphenols, tannins, and antioxidant activity were lower in two-stage than in single-stage fermented and unfermented rice bran. Conclusion: Single-stage fermentation with Ch. crassa improved nutritional characteristics of agro-industrial by-products. [J Adv Vet Anim Res 2018; 5(4.000): 472-480]

Objective: The present study was aimed to isolate and identify the egg-borne bacteria from different parts of duck eggs such as egg shell (outer and inner), yolk and albumen, and to assess the anti-biogram profile of the isolated bacteria. Materials and methods: A total of 40 samples were collected randomly from different grocery shops of Bangladesh Agricultural University (BAU) Campus and Kaowatkhali, Mymensingh. Following necessary preparation, the samples were streaked onto various selective media like Salmonella-Shigella (SS) agar (for Salmonella spp.), Eosin Methylene Blue (EMB) (for E. coli), and Mannitol Salt (MS) agar (for Staphylococcus spp.) respectively for isolation of bacteria. The bacteria were confirmed based on cultural and biochemical characteristics. Antibiotic sensitivity test of the bacterial isolates was performed using seven antibiotics (Ampicillin, Chloramphenicol, Ciprofloxacin, Gentamicin, Vancomycin, Kanamycin and Cephalexin) by following disc diffusion method. Results: E. coli, Staphylococcus spp. and Salmonella spp. were isolated and identified from the duck egg samples. Prevalence of E coli in outer egg shell was 80%, whereas in inner egg shell and inner egg content, this prevalence was 20% and 10%, respectively. Similarly, the prevalence of Staphylococcus spp. was 75%, 17.5% and 7.5% in outer egg shell, inner egg shell and inner egg content, respectively. The prevalence of Salmonella spp. was 82.5% in outer egg shell, 22.5% in inner egg shell and 12.5% in inner content of egg. All these three bacterial isolates were sensitive to Ciprofloxacin and Gentamicin and resistant to Ampicillin and Cephalexin. Conclusion: The duck eggs harbor multi-drug resistant (MDR) bacteria which may impose public health hazards if these MDR bacteria are transferred to human through food chain. [J Adv Vet Anim Res 2018; 5(2.000): 110-116]

Objective: The present study was initiated to ascertain the level of shedding of salmonellae by dogs in Makurdi area and to highlight the risk of infection for dog-owners. Materials and Methods: Rectal swabs from 200 dogs from different locations in the studyarea were examined in the study. The samples were cultured for salmonellae using Rappaport-Vassiliadis enrichment broth (Oxoid) and brilliant green agar (Oxoid). Suspected Salmonella isolates were serologically identified. Results: Overall, Salmonellae organisms were isolated from 11 (5.5%) of the 200 dogs sampled. Prevalence rates of 5.6% and 4.5% were recorded for apparently healthy and clinically sick dogs, respectively. Salmonella was respectively isolated from 4.1% to 9.1% of male and female dogs. Dogs aged 4 years and above recorded the highest prevalence rate. The study revealed a low prevalence rate in Nigerian local breed (mongrels) and high prevalence rates in exotic breeds of dogs. Conclusion: The isolation of salmonellae in apparently healthy and clinically sick dogs in this study indicates a carrier status which may constitute a serious problem in disease control in the study area. The lower prevalence rate of Salmonella infection in mongrels could be an indication of resistance to Salmonella in local breeds of dogs and should generate interest in research in the pathogenicity and pathogenesis of salmonellae in mongrels. [J Adv Vet Anim Res 2018; 5(4.000): 405-409]