خيارات البحث
النتائج 91 - 100 من 662
Virulence factors and antibiotic resistance of avian pathogenic Escherichia coli in eastern China
2019
Xu, Xiaojing | Sun, Qing | Zhao, Lixiang
Avian pathogenicEscherichia coli (APEC) causes serious colibacillosis and significant economic losses. Data on profiles of virulence factors and antibiotic resistances among APEC strains are crucial to the control of infection. In this study, strains were isolated from eastern China, and the prevalence of virulence factors and distribution of antibiotic resistance were determined. APEC strains were isolated and characterised by PCR for O serogroups, virulence factor genes, antibiotic resistance, and phylogenetic groups. O78 was the most prevalent serogroup and type A was the most frequent phylogenetic group. ThefimH,feoB, andiron genes were the most prevalent among the isolates. All isolates were multiresistant, and all strains were resistant to ampicillin and tetracycline, which are widely used in the poultry industry in China. This study provided important data on the presence of virulence genes and antibiotic resistance profiles of APEC from poultry farms in eastern China.
اظهر المزيد [+] اقل [-]Elaboration of triplex PCR for detection of selected viral infections in waterfowl
2019
Kozdruń Wojciech | Czekaj Hanna | Styś-Fijoł Natalia | Piekarska Karolina | Samanta Niczyporuk Jowita
Viral infections are the greatest threat to waterfowl and cause significant economic losses. Diagnosis and differentiation of three goose viruses is difficult in the field and often requires laboratory confirmation. Therefore, the aim of the study was to develop a triplex PCR and optimise its parameters for simultaneous detection of DNA of goose parvovirus (GPV), goose polyomavirus (GHPV), and goose circovirus (GoCV).
اظهر المزيد [+] اقل [-]Pathomorphological studies on wild boars infected with Metastrongylus spp., Ascarops strongylina, and Macracanthorhynchus hirudinaceus
2019
Panayotova-Pencheva Mariana | Todorova Katerina | Dakova Vassilena
Introduction: Pathomorphological changes in the lungs, stomach, and small intestines of wild boars infected with Metastrongylus spp., Ascarops strongylina, and Macracanthorhynchus hirudinaceus were investigated. Material and Methods: Dissection of 11 wild boars was performed, and parasitised organs were histologically investigated by common techniques. Results: Macroscopic lesions in the lungs infected with Metastrongyus spp. were seen within the apical parts of the large lobes, irregular in form, pale greyish in colour, and compact in consistency. The main pathohistological findings were: the presence of parasite forms, and lymphocytes and neutrophils in the lumen of bronchi and bronchioles, desquamation of the bronchial and bronchiolar epithelium, emphysema, thickening of alveolar septa, hyperaemia, alveolitis, infiltration of the interstitial tissue with giant cell, monocytes and eosinophils, and peribronchial and disseminated lymphoid hyperplasia. The principal observations accompanying infection with A. strongylina were inflammation and focal mucosal damage in the stomach, the latter clearly demarcated from the surrounding tissues. Severe injuries in the place of attachment of M. hirudinaceus to the wall of the small intestine were seen. Intestinal villi, underlying mucosa, and submucosa were destroyed, and an intense inflammatory reaction was present. Conclusion: The histopathological lesions showed wide diversity, varying from mild to severe; but none of them were lethal.
اظهر المزيد [+] اقل [-]Hyponatraemia and syndrome of inappropriate antidiuretic hormone secretion in non-azotaemic dogs with babesiosis associated with decreased arterial blood pressure
2019
Gójska-Zygner Olga | Bartosik Justyna | Górski Paweł | Zygner Wojciech
A previous study on canine babesiosis showed low serum tonicity in affected dogs, which may result from syndrome of inappropriate antidiuretic hormone secretion (SIADH). This endocrine disorder was recognised in human malaria which is considered a disease with similar pathogenesis to canine babesiosis. The aim of this study was to investigate the occurrence of SIADH in babesiosis-afflicted dogs.
اظهر المزيد [+] اقل [-]Serological diagnosis of lentivirus infection in goats raised in Algeria
2019
Idres Takfarinas | Lamara Ali | Temim Soraya | Boudjellaba Sofiane | Gagnon Jean | Chebloune Yahia
Introduction: Infection of goats with caprine arthritis encephalitis virus (CAEV) has been detected in variable proportions in many countries all over the world. Here, we investigated the seroprevalence of CAEV in goats raised in Algeria.
اظهر المزيد [+] اقل [-]Applicability of thyroxine measurements and ultrasound imaging in evaluations of thyroid function in turtles
2019
Pajdak-Czaus Joanna | Terech-Majewska Elżbieta | Będzłowicz Dagmara | Mączyński Martyn | Krystkiewicz Wioletta | Łabuć Sebastian | Platt-Samoraj Aleksandra | Szweda Wojciech
Introduction: The thyroid and parathyroid glands play a major role in maintaining physiological homeostasis in all vertebrates. Reptiles have plasma concentrations of thyroid hormones far lower than mammals. Low levels of these hormones in reptiles impede thyroid hormone detection with assays designed for the higher levels of mammals. The aim of this study was to explore teaming this with ultrasound imaging of the thyroid to appraise glandular function. Material and Methods: Thyroid function of four pond sliders was evaluated based on the results of T4 analyses and ultrasound. Results: The concentrations of T4 varied considerably between the examined animals from <9 nmol/L to >167.3 nmol/L. Ultrasound examination revealed uniform echogenicity and a smooth outline of the thyroid gland in all animals. Conclusion: Monitoring of thyroid function based on T4 and electrolyte concentrations is helpful in assessing the health and living conditions of reptiles, which is important in veterinary practice but problematic. Ultrasound examinations are useful in diagnosing changes in gland structure, such as tumours and goitres, and a combination of both methods supports comprehensive assessments of the anatomy and function of the thyroid gland.
اظهر المزيد [+] اقل [-]Experimental infection with T. canis and T. leonina in farm mink (Neovison vison)
2019
Klockiewicz Maciej | Jakubowski Tadeusz | Sobczak-Filipiak Małgorzata | Bartosik Justyna | Długosz Ewa
Introduction: Farm mink (Neovison vison) can be naturally exposed to T. canis and T. leonina pathogens on the farm. If mink were hosts, it would imply some veterinary public health as well as animal welfare issues. For this reason, the aim of the study was to determine whether mink might be definitive or paratenic hosts of these parasites. Material and Methods: Four groups of mink were infected with both parasite species using larvated eggs or feed containing mouse tissue previously infected with the parasites. Following inoculation, the infections were monitored in vivo by faecal examination for 14 weeks p.i., and then western blotting and ELISA were performed. Results: Coprology did not reveal any canine roundworm eggs, neither were nematodes found in mink intestines during post mortem examination. The specific IgG antibodies recognising excretory/secretory (ES) antigens of both parasite species were identified in mink sera. Single T. leonina tissue larvae were found in digested organs. Conclusions: Our results confirm that farm mink may contribute both T. canis and T. leonina infections. It was proved that farm mink were not their definitive hosts, and therefore mink faeces need not be considered a source of canine roundworm eggs in any soil it fertilises. Nonetheless, as farm mink may be a paratenic host for both parasite species, this may have some impact on the health and welfare of infected animals.
اظهر المزيد [+] اقل [-]Marine tetrodotoxin as a risk for human health
2019
Madejska Anna | Michalski Mirosław | Osek Jacek
Tetrodotoxin (TTX) is a toxin mainly occurring naturally in contaminated puffer fish, which are a culinary delicacy in Japan. It is also detected in various marine organisms like globefish, starfish, sunfish, stars, frogs, crabs, snails, Australian blue-ringed octopuses, and bivalve molluscs. TTX is produced by marine bacteria that are consumed mainly by fish of the Tetraodontidae family and other aquatic animals. TTX poisoning through consuming marine snails has recently begun to occur over a wider geographical extent through Taiwan, China, and Europe. This neurotoxin causes food intoxication and poses an acute risk to public health. The aim of this review is to present the most recent information about TTX and its analogues with particular regard to toxicity, methods of analysis, and risk to humans of exposure.
اظهر المزيد [+] اقل [-]Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis
2019
Tylkowska Agnieszka | Pilarczyk Bogumiła | Pilarczyk Renata | Zyśko Michał | Tomza-Marciniak Agnieszka
Introduction: Foxes are a reservoir of parasites that are dangerous to humans. The aim of the study was to determine the parameters associated with the occurrence of tapeworms in red foxes in north-western Poland.
اظهر المزيد [+] اقل [-]Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model
2019
Jodełko Agnieszka | Szymańska-Czerwińska Monika | Kycko Anna | Niemczuk Krzysztof
Q fever (coxiellosis) is an infectious disease of animals and humans, caused by.C. burnetii and widely distributed throughout the world. It is known that people and animals acquire the disease predominantly.via inhalation of infectious aerosols. The possibility of transmission of the pathogen by the alimentary route is still a matter of debate and remains controversial. Therefore the aim of this study was to fill the gaps in knowledge of oral transmission of.C. burnetii by conducting biological tests on the guinea pig model.
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