To create an industrial hemp variety of the Central European ecological and geographical type with a high cannabigerol content and universal application. Methods. Breeding (self-pollination, creation of artificial populations, selection), field, biochemical (thin-layer and gas-liquid chromatography of cannabinoid compounds), instrumental and technological assessment of fibre quality, and statistical methods. Results. Variety ‘Vik 2020’ was obtained as a result of creation of artificial populations. The plants are characterized by higher content of cannabigerol (1.034 ± 0.0323%), and almost zero of other secondary metabolites, such as cannabidivarin, cannabidiol, cannabichromene and psychotropic tetrahydrocannabinol (0.003 ± 0.0011; 0.018 ± 0.0080; 0.012 ± 0.0027, and 0.005 ± 0.0012%, respectively). The t rait of cannabigerol content is quite stable within the population and is not correlated with the trait of tetrahydrocannabinol content (r = -0.23). TLC showed that cannabigerol accumulated mainly in the form of cannabigerolic acid and to a lesser extent as a neutral compound, which is consistent with the theory that this substance is a precursor for the synthesis of other cannabinoids. According to the results of the competitive variety test, when growing to obtain fibre and seeds, the variety features short height, specifically significantly lower total (206.4 cm) and technical stem length (135.6 cm) compared to the standard variety, significantly higher inflorescence length (70.8 cm), which determine the formation of the significant yield of biomass suitable for pharmaceutical use and high seed yield (0.98 t/ha). The yield of total fibre was the same as in the standard variety (29.0%), but its quality and technological value for primary processing were higher. The variety had a homogeneous sex structure, resistance to bioltic and abiotic environmental factors. Plants reached biological maturity in 116 days (BBCH 89). This cultivar is recommended for obtaining seeds, quality fiber and potentially cannabigerol (on condition of changes in legislation). Conclusions. The efficiency of using self-pollinating lines in breeding with their subsequent combining into a synthetic population and improving selection was proved by the case of a new variety of industrial hemp ‘Vik 2020’, characterized by an increased content of cannabigerol and the absence of psychotropic properties

To create an industrial hemp variety of the Central European ecological and geographical type with a high cannabigerol content and universal application. Methods. Breeding (self-pollination, creation of artificial populations, selection), field, biochemical (thin-layer and gas-liquid chromatography of cannabinoid compounds), instrumental and technological assessment of fibre quality, and statistical methods. Results. Variety ‘Vik 2020’ was obtained as a result of creation of artificial populations. The plants are characterized by higher content of cannabigerol (1.034 ± 0.0323%), and almost zero of other secondary metabolites, such as cannabidivarin, cannabidiol, cannabichromene and psychotropic tetrahydrocannabinol (0.003 ± 0.0011; 0.018 ± 0.0080; 0.012 ± 0.0027, and 0.005 ± 0.0012%, respectively). The t rait of cannabigerol content is quite stable within the population and is not correlated with the trait of tetrahydrocannabinol content (r = -0.23). TLC showed that cannabigerol accumulated mainly in the form of cannabigerolic acid and to a lesser extent as a neutral compound, which is consistent with the theory that this substance is a precursor for the synthesis of other cannabinoids. According to the results of the competitive variety test, when growing to obtain fibre and seeds, the variety features short height, specifically significantly lower total (206.4 cm) and technical stem length (135.6 cm) compared to the standard variety, significantly higher inflorescence length (70.8 cm), which determine the formation of the significant yield of biomass suitable for pharmaceutical use and high seed yield (0.98 t/ha). The yield of total fibre was the same as in the standard variety (29.0%), but its quality and technological value for primary processing were higher. The variety had a homogeneous sex structure, resistance to bioltic and abiotic environmental factors. Plants reached biological maturity in 116 days (BBCH 89). This cultivar is recommended for obtaining seeds, quality fiber and potentially cannabigerol (on condition of changes in legislation). Conclusions. The efficiency of using self-pollinating lines in breeding with their subsequent combining into a synthetic population and improving selection was proved by the case of a new variety of industrial hemp ‘Vik 2020’, characterized by an increased content of cannabigerol and the absence of psychotropic properties

To create an industrial hemp variety of the Central European ecological and geographical type with a high cannabigerol content and universal application.Methods. Breeding (self-pollination, creation of artificial populations, selection), field, biochemical (thin-layer and gas-liquid chromatography of cannabinoid compounds), instrumental and technological assessment of fibre quality, and statistical methods.Results. Variety ?Vik 2020? was obtained as a result of creation of artificial populations. The plants are characterized by higher content of cannabigerol (1.034 ? 0.0323%), and almost zero of other secondary metabolites, such as cannabidivarin, cannabidiol, cannabichromene and psychotropic tetrahydrocannabinol (0.003 ? 0.0011; 0.018 ? 0.0080; 0.012 ? 0.0027, and 0.005 ? 0.0012%, respectively). The t rait of cannabigerol content is quite stable within the population and is not correlated with the trait of tetrahydrocannabinol content (r = -0.23). TLC showed that cannabigerol accumulated mainly in the form of cannabigerolic acid and to a lesser extent as a neutral compound, which is consistent with the theory that this substance is a precursor for the synthesis of other cannabinoids. According to the results of the competitive variety test, when growing to obtain fibre and seeds, the variety features short height, specifically significantly lower total (206.4 cm) and technical stem length (135.6 cm) compared to the standard variety, significantly higher inflorescence length (70.8 cm), which determine the formation of the significant yield of biomass suitable for pharmaceutical use and high seed yield (0.98 t/ha). The yield of total fibre was the same as in the standard variety (29.0%), but its quality and technological value for primary processing were higher. The variety had a homogeneous sex structure, resistance to bioltic and abiotic environmental factors. Plants reached biological maturity in 116 days (BBCH 89). This cultivar is recommended for obtaining seeds, quality fiber and potentially cannabigerol (on condition of changes in legislation).Conclusions. The efficiency of using self-pollinating lines in breeding with their subsequent combining into a synthetic population and improving selection was proved by the case of a new variety of industrial hemp ?Vik 2020?, characterized by an increased content of cannabigerol and the absence of psychotropic properties | ???????? ???? ??????????? ???????? ????????????????????? ???????-????????????? ???? ??????????????? ??????? ?????????????? ???????????? ? ??????????? ??????? ????????????.??????. ?????????? (?????????????, ????????? ??????????? ?????????, ?????), ???????, ?????????? (??????????? ?? ??????????? ????????????? ????????????? ??????), ???????????????-???????????? ?????????? ?????? ???????, ???????????.??????????. ? ?????????? ??????????? ???????? ???????? ???? ?????2020?, ??????? ????? ???????????????? ?????????? ??????? ???????????? (1,034???0,0323%), ?? ??? ????? ??????????? ????????????, ? ????? ?????? ??????????? ????? ????????? ??????????? ? ??????????????, ???????????, ????????????? ? ????????????? ???????????????????? (0,003???0,0011; 0,018???0,0080; 0,012???0,0027 ? 0,005???0,0012% ??????????). ?????? ?????? ???????????? ? ?????? ?????????? ? ????? ????????? ? ?? ??????????????? ? ??????? ?????? ???????????????????? (r =?-0,23). ?????????? ???????????? ????????????? ????????, ?? ??????????? ????????????? ????????? ? ????? ??????????????? ??????? ? ?????? ????? ?? ?????????? ???????, ?? ?????? ???????????? ? ???????, ?????? ? ???? ?? ???????? ? ???????????? ??? ??????? ????? ????????????. ?? ???????????? ??????????? ????????????????? ? ???? ??????????? ??? ????????? ??????? ? ??????? ???? ??????? ??????????????, ? ???? ??????? ????? ????????? ?? ??????-?????????? ????????? ????????? (206,4???) ? ????????? ??????? ??????? (135,6 ??), ? ??????? ?????? ??????????? ??????? ???????? (70,8 ??), ??? ???????????? ?????????? ??????? ???????, ????????? ??? ???????????? ?? ????????????? ????, ?? ??????????? ??????? (0,98??/??). ????? ?????????? ??????? ????????, ?? ? ? ?????-?????????, 29,0%, ??? ???? ????????????????? ????? ?????? ? ????????????? ???????? ?? ?????????? ????????????. ???? ??? ????????? ??????? ?????????, ????????? ?? ???- ?? ?????????? ???????? ??????????. ???????????? ?????? ?? ??????????? ????????? (BBCH 89) ??116 ???. ?????????????? ??? ??????????? ? ????? ????????? ???????, ???????? ??????? ? ?????????? ? ???????????? (?? ????? ????? ?????????????).????????. ???????? ???????????? ????????? ?? ???????????? ??????? ????????????? ????? ?? ????????? ?? ??????????? ? ?????????? ????????? ? ?????????????? ??????? ?? ???????? ?????? ????? ??????????? ???????? ???? 2020?, ?? ???????????????? ?????????? ??????? ???????????? ? ??????????? ???????????? ????????????.

Purpose. Determining prospects of introducing Callistephus chinensis cultivars of foreign breeding for further cultivation under conditions of the Central Forest-Steppe zone of Ukraine. Methods. Field, laboratory, analytical and statistical ones. Results. Ten C. сhinensis cultivars of theGerman breeding were tested under conditions of the Central Forest-Steppe zone of Ukraine. Biomorphological traits of the varieties of five sort types were identified, and their comparative analysis was conducted. Seasonal dynamics of growth and development of cultivars in the growing area was studied. Conclusions. Cultivars with short and mean duration phases of development can be grown under conditions of the Central Forest-Steppe zone of Ukraine by direct sowing that does not affect their seed productivity and sowing quality of seeds. Seeds of late cultivars can reach complete maturity only in case of seedling use. C. chinensis cultivars of the German breeding have successfully passed the initial test for suitability to introduction into the studied area.

Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and affordability for fungal species identification. However, Sanger sequencing fails to identify mixed ITS sequences in the case of mixed infections. To overcome this limitation, different high-throughput sequencing technologies have been explored. The nanopore-based technology is now one of the most promising long-read sequencing technologies on the market as it has the potential to sequence the full-length ITS region in a single read. In this study, we established a workflow for species identification using the sequences of the entire ITS region generated by nanopore sequencing of both pure yeast isolates and mocked mixed species reads generated with different scenarios. The species used in this study included Candida albicans (n = 2), Candida tropicalis (n = 1), Nakaseomyces glabratus (formerly Candida glabrata) (n = 1), Trichosporon asahii (n = 2), Pichia kudriavzevii (formerly Candida krusei) (n = 1), and Cryptococcus neoformans (n = 1). Comparing various methods to generate the consensus sequence for fungal species identification, the results from this study indicate that read clustering using a modified version of the NanoCLUST pipeline is more sensitive than Canu or VSEARCH, as it classified species accurately with a lower abundance cluster of reads (3% abundance compared to 10% with VSEARCH). The modified NanoCLUST also reduced the number of classified clusters compared to VSEARCH, making the subsequent BLAST+ analysis faster. Subsampling of the datasets, which reduces the size of the datasets by approximately tenfold, did not significantly affect the identification results in terms of the identified species name, percent identity, query coverage, percentage of reads in the classified cluster, and the number of clusters. The ability of the method to distinguish mixed species within sub-populations of large datasets has the potential to aid computer analysis by reducing the required processing power. The herein presented new sequence analysis pipeline will facilitate better interpretation of fungal sequence data for species identification.

Purpose. Determining prospects of introducing Callistephus chinensis cultivars of foreign breeding for further cultivation under conditions of the Central Forest-Steppe zone of Ukraine. Methods. Field, laboratory, analytical and statistical ones. Results. Ten C. сhinensis cultivars of theGerman breeding were tested under conditions of the Central Forest-Steppe zone of Ukraine. Biomorphological traits of the varieties of five sort types were identified, and their comparative analysis was conducted. Seasonal dynamics of growth and development of cultivars in the growing area was studied. Conclusions. Cultivars with short and mean duration phases of development can be grown under conditions of the Central Forest-Steppe zone of Ukraine by direct sowing that does not affect their seed productivity and sowing quality of seeds. Seeds of late cultivars can reach complete maturity only in case of seedling use. C. chinensis cultivars of the German breeding have successfully passed the initial test for suitability to introduction into the studied area.

The article reflects the progress of genetic decrease of Rye F 3k- 10029/ Saratovske (Саратовське) 4 height by means of the shortest stem plants selection during the period from 1974 to 2010. 37 years selection of the shortest- stern genotypes decreased the plants height from 119.33 cm to 22.57cm. Targeted selection into minus direction decreased the plants height in 5,29 times on the background of the dominant HI expression. In average, the height of plants in the course of 27 breeding cycles were decreasing by 2.69 cm, but that was not going gradually. A new donor Gnom 3 had been created for ultra short-stem trait of the Winter Rye, with the marking of alleles HI-3HI-3. Relative influence on the minus selection efficiency has been established by height of plants for the selection differential (38%) and coefficient of inheritance in narrow sense (14,56%). Realized efficiency of selection in decrease of winter rye height in 72,08% of cases corresponded to predicted hit ration of the breeding. Analyzes of genetic and statistical parameters and correlation clusters of 11 utilitarian average characteristics of ultra short- stem rye Gnom 3 for the period of 1974 to 2010 has been performed.

Purpose. To evaluate hazelnut cultivars, species and hybrids from the genetic collection of Corylus spp. in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine for the complex of economic characters. An attempt has been made to analyze the information on Corylus spp. identity, taxonomy and description, dissemination and ecological requirements of the species, possibilities to use the genetic potential for developing new cultivars. Methods. The value of the Corylus spp. collection representatives was investigated using conventional testing procedures. For summarizing information concerning phylogenetic reconstruction of the Corylus L. genus and hazelnut, a number of scientific publications to be proposed for discussion was analyzed. The oil content in hazelnut kernels and the fatty acid composition was determined using official methods. Results. The best samples of hazelnut genetic collection were included into the broad hybridization programme, and C. chinensis Franch. representatives as well. A number of hybrid seedlings was obtained including new hazelnut cultivars ‘Sofiyivsky 1’, ‘Sofiyivsky 2’ and ‘Sofiyivsky 15’ which were characterized by spherical or almost spherical fruits, high winter hardiness and drought resistance, as well as the absence of rhythmicity in fruiting. Conclusions. The collection of varieties, forms, cultivars and species of the Corylus L. genus created during the last years can be the base for hazelnut breeding in Ukraine.

Мета. Серед описаних у фаховій літературі ідентифікованих генів стійкості проти збудника бурої іржі виділити чужо­рідні, інтрогресовані у вид Triticum aestivum L. джерела, встановити їх походження та перспективи використання в селекційній практиці. Результати. Пшениця озима м’яка як основна зернова культура належить до групи рослин, яких найдавніше вирощують у контрольованих умовах. Протягом періоду вегетації вона зазнає згубного впливу збудників хвороб, тому пошук джерел стійкості проти них є першочерговим завданням селекції. Бура іржа – одна з найпоширеніших і шкодочинних хвороб пшениці. Вона призводить до значних втрат урожаю та погіршення якості зерна. Популяція збудника Puccinia reconditа вирізняється неабиякою адаптивною здатністю. Висока варіабельність вірулентності гриба призводить до накопичення патотипів, здатних долати гени стійкості пшениці. Найбільш екологічно безпечним методом контролювання захворювання є створення стійких сортів. Ефективність селекції на стійкість проти бурої іржі можна покращити, використовуючи різні Lr-гени стійкості. На цей час у геному пшениці та її родичів ідентифіковано й охарактеризовано за хромосомною локалізацією та ефективністю понад 90 (Lr) генів стійкості проти цього збудника. Виявлено, що майже всі ефективні на території України гени стійкості проти збудника бурої іржі, окрім Lr10 та Lr23, є чужорідними, перенесеними в Triticum aestivum від інших видів: Aegilops speltoides – гени Lr28, Lr35, Lr36, Lr47, Lr51, Lr66; Aegilops tauschii – Lr1, Lr21, Lr22а, Lr32, Lr39, Lr42; Triticum timopheevii – Lr18 та Lr50; Thinopyrum elongatum – Lr19, Lr29, Lr24; Secale cereale – Lr25, Lr26 та Lr45; Aegilops umbellulata – Lr9, Lr76; Triticum speltа – Lr44, Lr65, Lr71; Triticum dicoccoides – Lr53, Lr64; Aegilops triuncialis – Lr58, LrTr; Tr. timopheevii spp. viticulosum – LrTt1; Aegilops ventricosa – Lr37; Aegilops kotschyi – Lr54; Elymus trachycaulis – Lr55; Aegilops sharonensis – Lr56; Aegilops geniculate – Lr57; Aegilops peregrine – Lr59; Triticum turgidum – Lr61; Aegilops neglecta – Lr62; Triticum monococcum – Lr63. Висновки. Залучення до схрещувань культурних та диких видів родичів пшениці дасть змогу отримати неоднорідний за стійкістю проти збудника бурої іржі селекційний матеріал.

Purpose. To evaluate hazelnut cultivars, species and hybrids from the genetic collection of Corylus spp. in the National Dendrological Park “Sofiyivka” of the NAS of Ukraine for the complex of economic characters. An attempt has been made to analyze the information on Corylus spp. identity, taxonomy and description, dissemination and ecological requirements of the species, possibilities to use the genetic potential for developing new cultivars. Methods. The value of the Corylus spp. collection representatives was investigated using conventional testing procedures. For summarizing information concerning phylogenetic reconstruction of the Corylus L. genus and hazelnut, a number of scientific publications to be proposed for discussion was analyzed. The oil content in hazelnut kernels and the fatty acid composition was determined using official methods. Results. The best samples of hazelnut genetic collection were included into the broad hybridization programme, and C. chinensis Franch. representatives as well. A number of hybrid seedlings was obtained including new hazelnut cultivars ‘Sofiyivsky 1’, ‘Sofiyivsky 2’ and ‘Sofiyivsky 15’ which were characterized by spherical or almost spherical fruits, high winter hardiness and drought resistance, as well as the absence of rhythmicity in fruiting. Conclusions. The collection of varieties, forms, cultivars and species of the Corylus L. genus created during the last years can be the base for hazelnut breeding in Ukraine.

Cytospora species are destructive canker and dieback pathogens of woody hosts in natural and agroecosystems around the world. In this genus, molecular identification has been limited due to the paucity of multi-locus sequence typing studies and the lack of sequence data from type specimens in public repositories, stalling robust phylogenetic reconstructions. In most cases a morphological species concept could not be applied due to the plasticity of characters and significant overlap of morphological features such as spore dimensions and fruiting body characters. In this study, we employed a molecular phylogenetic framework with the inclusion of four nuclear loci (ITS, translation elongation factor 1-alpha, actin, and beta-tubulin) to unveil the biodiversity and taxonomy of this understudied important genus of plant pathogens. Phylogenetic inferences based on 150 Californian isolates revealed 15 Cytospora species associated with branch and twig cankers and dieback of almond, apricot, cherry, cottonwood, olive, peach, pistachio, plum, pomegranate, and walnut trees in California. Of the 15 species recovered in this study, 10 are newly described and typified, in addition to one new combination. The pathogenic status of the newly described Cytospora species requires further investigation as most species were associated with severe dieback and decline of diverse and economically important fruit and nut crops in California.