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Apomixis and the problem of obtaining haploids and homozygote diploids in pear (Pyrus communis L.)
2013
Долматов, Є. О | Джафарова, В. Є
The article highlights results of research over simulative apomixes in pear and its utilization for obtaining haploids and homozygote diploids. It has been established that over 50% pear varieties with failed remote hybridization are capable of generating seeds of apomictic origin producing diploid plants. Genotypes displaying maximal inclination to apomixes have been singled out. Apomictic pear seedlings obtained from foreign pollination within the limits of the same combination are inherent in profound morphological diversity. Fruit-bearing apomicts originated from one and the same maternal plant differ to the same extent as hybrid seedlings of the same family. Genetic markers have enabled to establish that these are embryo sacs in which meiosis has completed that give rise to apomictic seeds. In vitro method as used for the purpose of increasing apomictic plants output has been illustrated. The greatest induction of apomictic shoots in vitro has been reached by alternation of BAP cytokinin at concentration of 1mg/l and 2 mg/l on the background of GA3 amounting to 1,5 mg/l. Grafting with shoots in vitro on non-sterile rootstocks of pear (Pyrus communis) has increased the output of plants up to 80%. A cytological assessment of 9 apomictic samples is provided. The cytological analysis of samples of apomictic forms has certified the presence of simulative haploid parthenogenesis in pear.
اظهر المزيد [+] اقل [-]Peculiaritis of Apomictic plants development Pyrus communis х Chaenomeles japonica (Thunb.) Lindl. in vitro conditions
2013
Джафарова, В. Є | Голишкін, Л. В | Долматов, Є. О | Ташматова, Л. В
The development features of four samples of apomictic plants during micropropagation are presented. These plants have been derived from embryos after pear pollination with the pollen of Chaenomeles japanica at 55 and 70 days of their development. Different responses of samples on the tested concentration of cytokinin (6BAP) and its combination with gibberell acid are shown. Tendency of increasing of the coefficient of the reproduction of separate numbers of apomictic plants has been noted under changing of BAP concentration from 1 mg/l to 2 mg/l or BAP combination with GA3. For the purpose of optimization of the stage of conglomeration forming (buds and shoots) BAP concentration 1 and 2 mg/l should be alternated in a passage on the background of GA3 1,5 mg/l. For the first time the origin of apomictic plant roots during rhizogenesis has been retraced and the anatomical structure of roots in conditions in vitro has been studied. Root formation in vitro occurs in internal tissues of a shoot. Roots of apomictic plants formed in vitro are primary as in plants developed in vivo.
اظهر المزيد [+] اقل [-]Aroma peculiarities of apricot (Armeniaca vulgaris Lam.) and cherry-plum (Prunus cerasifera Ehrh.) flowers
2013
Горіна, В. М | Ріхтер, О. О | Виноградов, Б. О
In the component composition of volatile solutions determining fragrance of the flowers in apricot and cherry-plum varieties and Prunus brigantiaca Vill. x Armeniaca vulgaris Lam. hybrids there are 36 highest hydrocarbons and benzaldehyde that prevail. There are fewer amounts of the solutions which scare bees (benzaldehyde) in the fragrance of cherry-plum varieties as compared to the flowers of apricot and hybrids. At the same time, the content of tricosane, pentacosane, docosane, heneycosane, eicosane, nonadecan that probably attract bees is higher in the cherry-plum flowers than in the fragrance of apricot and hybrid flowers. The average three years yield of cherry-plum plants (Nikitska Zhovta 10,7 and Salgirskaya Rumjanaya 28,5 t/ ha) is higher than for apricot (Recolte de Schatene 0,3; Rodnik 2,9; Ananasniy Tsurupinsky 7,4 t/ha) and hybrids (8110 – 5,2; 8098 – 6,4 t/ha) that could be explained with better pollination of flowers and better fruit formation. Prevailing components of flower aroma of these plants and their possible link with yield of the objects in questions have been analyzed.
اظهر المزيد [+] اقل [-]Genetic control over characteristic of 1000 seeds weight in hybrids of sugar beet and combining ability of crossbreeding components
2012
Корнєєва, М. О | Ненька, О. В | Ненька, М. М
The article shows experimental data of interline hybrids of sugar beet on the basis of weight of 1000 seeds, genetic determination of characteristic based on the method of Hayman and topcrossed polytested crossbreeding is established, their comparison is made. It also highlights valuable hybrid combinations and valuable for combining pollinating lines used in the breeding to enhance properties of sowing seeds.
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