Purpose. To obtain Miscanthus sacchariflorus (Maxim.) Hack and Miscanthus sinensis Andersson in vitro culture by indirect morphogenesis. Methods. Biotechnological procedures, mathematical and statistical analyses. Results. Composition of nutrient medium was developed intended for induction of callusogenesis from Miscanthus seeds with a poor germination and viability of seedlings – Murashige and Skoog (MS) medium was modified for the amount of macroelements (half-dose) that was supplemented with amino acids (300 mg/l of glutamic acid, 50 mg/l of aspartic acid, 5 mg/l of tyrosine, 3 mg/l of arginine, 2 mg/l of hydroxyproline) and plant growth regulators [2,5 mg/l of 2.4D (2.4-Dichlorophenoxyacetic acid), 0,6 mg/l of BAP (6-Benzyl-aminopurine) and 0,3 mg/l of ABA (Abscisic acid)]. Composition of nutrient medium was developed for regeneration of microplants from callus – agar MS medium was modified for the amount of macroelements (half-dose) supplemented with vitamins: 10 mg/l of thiaminum, 1,0 mg/l of pyridoxine, 1,0 mg/l of nicotinic acid (by White), 1,0 mg/l of ascorbic acid, 250 mg/l of glutamic acid, 2,0 mg/l of BAP, 0,3 mg/l of NAA (Naphthaleneacetic acid). On this medium, 100% regeneration of M. sacchariflorus (Maxim.) Hack and 50% regeneration of M. sinensis Andersson was obtained. Due to media modification aimed at initiating callusogenesis and microplants regeneration, reproduction factor of M. sinensis was increased 20 times at the average, M. sacchariflorus – 35–40 times. Conclusions. Plants of M. sacchariflorus (Maxim.) Hack and M. sinensis Andersson were obtained in vitro culture by initiation of callusogenes and microplants regeneration from the Miscanthus seeds with poor germination and viability on nutrient media of certain composition.

Purpose. Theoretical and experimental substantiation of introduction of “Romantica” roses originated by Meilland company under the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. Methods. Biometrical, comparative-morphological, statistic ones. Results. Viability, growth and development indices as well as ornamental qualities (tolerance for winter conditions, frost hardiness, drought resistance, complex disease and pest resistance, habit maintenance, shoot formation, flowering, coloration, shape of flowers, petal number etc.) were analyzed for 12 rose cultivars of “Romantica” series planted on the collection and exhibition plots of the National Dendrological Park «Sofiivka» NAS of Ukraine. The investigations were conducted within 2012–2015. The most of the cultivars got the mark of introduction success 41–45 points of 50; so, the cultivars are totally promising for introduction into the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. ‘Leonardo da Vinci’ and ‘Yves Piaget’ cultivars got point number 35–38 and they can be considered promising ones. The acclimatization number for the most of the investigated cultivars was 85–90 points of 100 that means good adaptation of the plants in the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. The adaptation of cv. ‘Leonardo da Vinci’ and cv. ‘Yves Piaget’ roses (point number 75) can be considered satisfactory. The total evaluation of “Romantica” roses ornamental qualities made 76–87 points of 100; so, all the cultivars display high ornamental qualities and deserve to be introduced to the production. Conclusions. The garden roses of “Romantica” series originated by Meilland company being introduced into the conditions of the Right-Bank Forest-Steppe Zone of Ukraine are adapted well in the introduction region and they are promising for the subsequent introduction.

500 O-type lines of sugar beet hybrid components were studied according to their main economically valuable characteristics, namely, one-seed structure, germination readiness, productivity, sugar content and sugar yield. 13 best features were determined, herewith one-seed structure of most tested genotypes was observed at the level of 92-99% which appeared to be of nearly the same level during all the years of the research. On that ground they were included into the program for further research. The weight of seeds of the researched numbers ranged from 13,3 to 22,2 gr. The productivity of the researched O-type lines varied from 40,1 to 45,2 tons per hectare. Herewith, the highest productivity index was observed in 664bk 6 line with the average index 42,7 tons per hectare which ensured sugar yield 7,59 tons per hectare. The results of the research into the influence of low temperatures on seed germination readiness and capacity of O-type sugar beet lines were given. All the researched materials readily respond to conditions of germination and the seed germination readiness and germination capacity directly depend on the temperature. However, high indices of seed germination readiness and capacity under low temperatures is an essential feature of parent breeding materials in the breeding process while creating new hybrids which can be grown by means of intensive technologies. This will make it possible to sow heterosis sugar beet hybrids in earlier terms. It was suggested to use the best lines as parent components for development of heterosis hybrids which are able to ensure seed germination under low temperatures.

500 O-type lines of sugar beet hybrid components were studied according to their main economically valuable characteristics, namely, one-seed structure, germination readiness, productivity, sugar content and sugar yield. 13 best features were determined, herewith one-seed structure of most tested genotypes was observed at the level of 92-99% which appeared to be of nearly the same level during all the years of the research. On that ground they were included into the program for further research. The weight of seeds of the researched numbers ranged from 13,3 to 22,2 gr. The productivity of the researched O-type lines varied from 40,1 to 45,2 tons per hectare. Herewith, the highest productivity index was observed in 664bk 6 line with the average index 42,7 tons per hectare which ensured sugar yield 7,59 tons per hectare. The results of the research into the influence of low temperatures on seed germination readiness and capacity of O-type sugar beet lines were given. All the researched materials readily respond to conditions of germination and the seed germination readiness and germination capacity directly depend on the temperature. However, high indices of seed germination readiness and capacity under low temperatures is an essential feature of parent breeding materials in the breeding process while creating new hybrids which can be grown by means of intensive technologies. This will make it possible to sow heterosis sugar beet hybrids in earlier terms. It was suggested to use the best lines as parent components for development of heterosis hybrids which are able to ensure seed germination under low temperatures.

The article highlights summary of initial material introduction and of generation of sterile form of sugar sorghum, its ground germination capacity and viability of the seed in various fractions. This has scrutinized impact of various chemical reagents and expositions the yield of sterile and viable explants.