Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of gene­tic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximi­zing their potential.

Purpose. Literature review of DNA banks creation as the current strategy of plant genetic resources conservation. Results. The current state of plant genetic resources conservation was analyzed in the context of the threat of gene­tic erosion. The importance of DNA banks was shown which function is to store DNA samples and associated products and disseminate them for research purposes. The main DNA banks in the world were described, including the Republican DNA Bank of Human, Animals, Plants and Microorganisms at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Stages of DNA banking were considered: tissue sampling (usually from leaves), cell destruction, DNA extraction, DNA storage. Different methods of tissue sampling, extraction and DNA storage were compared. The need for Plant DNA Bank creation in Ukraine was highlighted. Conclusions. DNA collections is an important resource in the global effort to overcome the crisis in biodiversity, for managing world genetic resources and maximi­zing their potential.

Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.

Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hyb­rids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activi­ty of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.

Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a confusion in the Ukrainian names of plants of Cydonia-Chaenomeles–Pseudocydonia, Mespilus–Eriobotrya, Amelanchier–Cotoneaster. Considering the division of Sorbus s.l. on separate genera Sorbus s.str., Aria, Cormus, Chamaemespilus, Torminalis and availability of numerous hybrids with their involvement, the Ukrainian names for both taxa and nothotaxa of species and generic levels are offered, principles of their choice are also considered. Formation of the Ukrainian generic names on the basis of specific epithets leads to undesirable tautonomy. Aspects of derussification of the Ukrainian professional terminology are discussed. Conclusions. Ukrainian scientific generic and nothogeneric plant names for subtribe Malinae are transliterated/transcripted, borrowed as a calque of latin names or have their own origin, consisting of one or two words. The choice of the best variants of generic and species names from a number of possible ones has a temporal component and largely depends on experts who apply them and basic principles of choice. It is necessary to improve the modern Ukrainian plant nomenclature on a national basis involving the transformed Latin scientific names for introduced species and hybrids.

Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a confusion in the Ukrainian names of plants of Cydonia-Chaenomeles–Pseudocydonia, Mespilus–Eriobotrya, Amelanchier–Cotoneaster. Considering the division of Sorbus s.l. on separate genera Sorbus s.str., Aria, Cormus, Chamaemespilus, Torminalis and availability of numerous hybrids with their involvement, the Ukrainian names for both taxa and nothotaxa of species and generic levels are offered, principles of their choice are also considered. Formation of the Ukrainian generic names on the basis of specific epithets leads to undesirable tautonomy. Aspects of derussification of the Ukrainian professional terminology are discussed. Conclusions. Ukrainian scientific generic and nothogeneric plant names for subtribe Malinae are transliterated/transcripted, borrowed as a calque of latin names or have their own origin, consisting of one or two words. The choice of the best variants of generic and species names from a number of possible ones has a temporal component and largely depends on experts who apply them and basic principles of choice. It is necessary to improve the modern Ukrainian plant nomenclature on a national basis involving the transformed Latin scientific names for introduced species and hybrids.

Purpose. Reviewing a history of formation and current state of the national nomenclature and streamlining the Ukrainian scientific species names for the genus Fragaria L. Results. Controversial attitudes towards the use of names «sunytsi» (= garden strawberry) and «polunytsi» (= hill strawberry) are inherently present in the Ukrainian nomenclature of the genus Fragaria L. The Ukrainian scientific names of species of this group of plants should be brought into line with the generic name “Sunytsi” in plural. Nothogeneric name x Fragophora Mezhenskyj (= Dasiphora Rafin. x Fragaria L.) is proposed. Conclusions. In a professional environment it is necessary to use only scientific names of species of the genus Fragaria – Sunytsi (= garden strawberry) that is used in plural in Ukrainian. Concerning garden strawberry, in every day life and popular literature the usage of a derivative sunytsia (in Ukrainian) in singular as well as polunytsia, polunytsi (in Ukrainian) may be permissible.

Purpose. Reviewing a history of formation and current state of the national nomenclature and streamlining the Ukrainian scientific species names for the genus Fragaria L. Results. Controversial attitudes towards the use of names «sunytsi» (= garden strawberry) and «polunytsi» (= hill strawberry) are inherently present in the Ukrainian nomenclature of the genus Fragaria L. The Ukrainian scientific names of species of this group of plants should be brought into line with the generic name “Sunytsi” in plural. Nothogeneric name x Fragophora Mezhenskyj (= Dasiphora Rafin. x Fragaria L.) is proposed. Conclusions. In a professional environment it is necessary to use only scientific names of species of the genus Fragaria – Sunytsi (= garden strawberry) that is used in plural in Ukrainian. Concerning garden strawberry, in every day life and popular literature the usage of a derivative sunytsia (in Ukrainian) in singular as well as polunytsia, polunytsi (in Ukrainian) may be permissible.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

Purpose. Considering ways to solve issues related to soil cover study at the institutions for plant varieties examination. Methods.Fieldandanalytical ones. Results. Issues are identified that arise during soil sampling at institutions for plant varieties examination within the state system of plant varieties protection. The author considered in detail the current soil sampling techniques for agrochemical tes­ting for humus, nitrogen (compounds to be easily hydrolyzed), mobile P2O5, exchangeable K2O, hydrolytic acidity and others. Attention was paid to violation of methodological requirements concerning the time of soil sampling and number of samples for agrochemical testing at the experimental field, to the lack of devices for meteorological observation and appliances for soil and leaf diagnostics. The article also touches upon the issues associated with the study of soil cover at fields with scientifically-based crop rotation that belong to institutions for plant varieties examination within the state system of plant varieties protection. Conclusions. It should be necessary to follow recommended practice when sampling at elementary sites for agrochemical testing and performing all operations in accordance with crop cultivation flowcharts. General recommendations for agrochemical testing of fields in case of conventional crop rotations in farms are not suitable for fields with scientifically-based crop rotations at institutions for plant varieties examination.

To analyse the modern classification and nomenclature of species of pome fruit crops which varieties are listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine, and improve terminological system of the Ukrainian names of both species and garden crops. Results. Fruit cultivars and most apple clonal rootstocks belong to Malus pumila, and ornamental cultivars belong to Malus gloriosa. The most common scientific name of the cultivated apple, especially among horticulturists, is Malus domestica, although according to the principle of priority the name Malus pumila should have the advantage. As far as Nomenclature Committee for Vascular Plants has rejected the proposal to conserve the name Malus domestica, Malus pumila is correct name for the cultivated apple. The use of synonymic name Malus domestica should be avoided in both scientific and scientific-popular papers for stability of nomenclature. Pear cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine are presented by Pyrus communis, and pear rootstocks – by Cydonia oblonga. Fruit cultivars of the latter belong to separate fruit crop named quince. An apple-quince hybrid was registered as universal clonal rootstock for pome fruit crops. The State Register of Plants Varieties Suitable for Dissemination in Ukraine also contains nonconventional fruit crops such as Chaenomeles and hawthorn that consist of some species and nothospecies. Conclusions. In scientific publications one should stop the use of synonymic name Malus domestica in favour of the correct name for cultivated apple Malus pumila. Apple, pears and Chaenomeles cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine have a complex multispecies origin whereas quince, hawthorn and pear roostock cultivars systematically are monospecies. A universal roootstock of pome fruit crops is Cydolus, or apple-quince, that resulted from interspecies hybridization between quince and apple. Refusal Refuse to use obsolete names of fruit plants species will promote harmonization of professional language.

To analyse the modern classification and nomenclature of species of pome fruit crops which varieties are listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine, and improve terminological system of the Ukrainian names of both species and garden crops. Results. Fruit cultivars and most apple clonal rootstocks belong to Malus pumila, and ornamental cultivars belong to Malus gloriosa. The most common scientific name of the cultivated apple, especially among horticulturists, is Malus domestica, although according to the principle of priority the name Malus pumila should have the advantage. As far as Nomenclature Committee for Vascular Plants has rejected the proposal to conserve the name Malus domestica, Malus pumila is correct name for the cultivated apple. The use of synonymic name Malus domestica should be avoided in both scientific and scientific-popular papers for stability of nomenclature. Pear cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine are presented by Pyrus communis, and pear rootstocks – by Cydonia oblonga. Fruit cultivars of the latter belong to separate fruit crop named quince. An apple-quince hybrid was registered as universal clonal rootstock for pome fruit crops. The State Register of Plants Varieties Suitable for Dissemination in Ukraine also contains nonconventional fruit crops such as Chaenomeles and hawthorn that consist of some species and nothospecies. Conclusions. In scientific publications one should stop the use of synonymic name Malus domestica in favour of the correct name for cultivated apple Malus pumila. Apple, pears and Chaenomeles cultivars listed in the State Register of Plants Varieties Suitable for Dissemination in Ukraine have a complex multispecies origin whereas quince, hawthorn and pear roostock cultivars systematically are monospecies. A universal roootstock of pome fruit crops is Cydolus, or apple-quince, that resulted from interspecies hybridization between quince and apple. Refusal Refuse to use obsolete names of fruit plants species will promote harmonization of professional language.

Purpose. To study morphometric parameters of fruits (cypselae) of purple coneflower (Echinacea purpurea (L.) Moench) of ‘Zirka Mykoly Vavylova’ cultivar and pale coneflower (Echinacea pallida (Nutt.) Nutt) of ‘Krasunia Prerii’ cultivar and determine the impact of agro-meteorological factors on their performance according to long-term data. Methods. Laboratory, Mathematics and Statistics. Results. It was found that the parameters of cypselae and its weight in various types of coneflowers varied considerably from year to year. In this context the indicators of cypselae width and thickness had a larger variability as compared with the length. Mean length of Echinacea purpurea ranged from 4,57 to 6,16 mm, width – 2,30–2,97 mm, thickness – 1,74–2,28 mm. Weight of a cypselae made up 4,40–6,50 mg. Length of a fruit of Echinacea pallida was 4,57–5,74 mm, width – 2,51–3,18 mm, thickness – 1,76–2,37 mm, and a fruit weight varied from 5,65 to 7,70 mg. The correlation analysis revealed reliable connection of agro-climatic parameters and the parameters of the fruit. Conclusions. Using long-term data, the morphology of cypselaes of two Echinacea species introduced to Ukraine were studied, their basic parameters and the variability of indicators, connection with agro-climatic factors that is necessary to consider in crop growing for seeds .

Purpose. To study morphometric parameters of fruits (cypselae) of purple coneflower (Echinacea purpurea (L.) Moench) of ‘Zirka Mykoly Vavylova’ cultivar and pale coneflower (Echinacea pallida (Nutt.) Nutt) of ‘Krasunia Prerii’ cultivar and determine the impact of agro-meteorological factors on their performance according to long-term data. Methods. Laboratory, Mathematics and Statistics. Results. It was found that the parameters of cypselae and its weight in various types of coneflowers varied considerably from year to year. In this context the indicators of cypselae width and thickness had a larger variability as compared with the length. Mean length of Echinacea purpurea ranged from 4,57 to 6,16 mm, width – 2,30–2,97 mm, thickness – 1,74–2,28 mm. Weight of a cypselae made up 4,40–6,50 mg. Length of a fruit of Echinacea pallida was 4,57–5,74 mm, width – 2,51–3,18 mm, thickness – 1,76–2,37 mm, and a fruit weight varied from 5,65 to 7,70 mg. The correlation analysis revealed reliable connection of agro-climatic parameters and the parameters of the fruit. Conclusions. Using long-term data, the morphology of cypselaes of two Echinacea species introduced to Ukraine were studied, their basic parameters and the variability of indicators, connection with agro-climatic factors that is necessary to consider in crop growing for seeds .

Мета. З’ясувати особливості формування адитивних біоценотичних зв’язків у фітоценозах тритикале озимого в умовах лісостепового та полісько-лісостепового екотопів. Методи. Польові, лабораторні дослідження та математично-статистичний аналіз. Результати. Досліджено чутливість генотипів тритикале озимого, зокрема АД 256, Славетне, Славетне поліпшене, Пшеничне, Чаян, Ягуар, Августо, ДАУ 5 і Вівате Носівське, на дію мікробних препаратів альбобактерину та діазобактерину в умовах лісостепового та полісько-лісостепового екотопів. Встановлено, що інтродукція агрономічно корисної мікробіоти в фітоценози тритикале озимого забезпечує формування адитивних біоценотичних зв’язків, ефективність яких визначає рівень продуктивності зернової культури. Висновки. Для забезпечення оптимальних цільових параметрів урожаю зерна в умовах лісостепового та полісько-лісостепового екотопів доцільно проводити передпосівну інокуляцію насіння тритикале озимого ефективними штамами мікробних препаратів – Azospіrіllum brasilense діазобактерину та Achromobacter album 1122 альбобактерину.

Мета. З’ясувати особливості формування адитивних біоценотичних зв’язків у фітоценозах тритикале озимого в умовах лісостепового та полісько-лісостепового екотопів. Методи. Польові, лабораторні дослідження та математично-статистичний аналіз. Результати. Досліджено чутливість генотипів тритикале озимого, зокрема АД 256, Славетне, Славетне поліпшене, Пшеничне, Чаян, Ягуар, Августо, ДАУ 5 і Вівате Носівське, на дію мікробних препаратів альбобактерину та діазобактерину в умовах лісостепового та полісько-лісостепового екотопів. Встановлено, що інтродукція агрономічно корисної мікробіоти в фітоценози тритикале озимого забезпечує формування адитивних біоценотичних зв’язків, ефективність яких визначає рівень продуктивності зернової культури. Висновки. Для забезпечення оптимальних цільових параметрів урожаю зерна в умовах лісостепового та полісько-лісостепового екотопів доцільно проводити передпосівну інокуляцію насіння тритикале озимого ефективними штамами мікробних препаратів – Azospіrіllum brasilense діазобактерину та Achromobacter album 1122 альбобактерину.

Purpose. To study interactions among the representatives of the genus Salix L. through the cluster analysis, form groups of closely related species and hybrid forms basing on differences of morphological parameters of leaves. Methods. Field, cluster analysis and tree graphics. Results. Willow species were grouped according to absolute parameters of leaf, and three groups of clusters were identified. The degree of affinity between species were assessed using values of an Euclidean distance. Distinctive features of leaf parameters were defined: length of a leaf blade (Ll), distance (cm) between the leaf tip and its maximum width (SDmxT) and the distance between the leaf tip (cm) and the line of its width that corresponds to the length of petiole (SLpT). Conclusions. Using the willow species collection as an example, diagnostically valuable quantitative parameters of leaves were revealed, the use of which allows to identify willow species and hybrid forms through PC applications.

Purpose. To study interactions among the representatives of the genus Salix L. through the cluster analysis, form groups of closely related species and hybrid forms basing on differences of morphological parameters of leaves. Methods. Field, cluster analysis and tree graphics. Results. Willow species were grouped according to absolute parameters of leaf, and three groups of clusters were identified. The degree of affinity between species were assessed using values of an Euclidean distance. Distinctive features of leaf parameters were defined: length of a leaf blade (Ll), distance (cm) between the leaf tip and its maximum width (SDmxT) and the distance between the leaf tip (cm) and the line of its width that corresponds to the length of petiole (SLpT). Conclusions. Using the willow species collection as an example, diagnostically valuable quantitative parameters of leaves were revealed, the use of which allows to identify willow species and hybrid forms through PC applications.