Purpose. To create new competitive spring wheat varieties. Methods. Field study, laboratory test. Results. Based on the competitive variety trial, bread spring wheat line ‘Lutescens 07-26’ has been selected due to high values of such traits as resistance to fungal diseases, grain qua­lity(protein content accounted for 15.0%), 1000 kernel weight (44.6 g) productivity (3.92 t/ha) and lodging resistance (9 points). In 2011, it was submitted to the State variety testing as ‘Panianka’ variety. Durum spring wheat line ‘Leukurum 08-11’ was characterized by a number of positive traits: quite a high productivity (3.05 t/ha), short stem (79 cm), resistance to fungal diseases and lodging(9 points), and in 2011 it was submitted to the State variety testing as ‘Diana’ variety. According to the results of the State variety testing in 2012–2014, spring wheat varieties ‘Panianka’ and ‘Diana’ in 2015 were put on the State Register of plant varieties suitable for dissemination in Ukraine. Conclusions. For farms in Forest-Steppe and Polissia zones of Ukraine, bread and durum spring wheat varieties were bred by V. M.Remeslo Myronivka Institute of Wheat of NAAS of Ukraine that demonstrated rather high potential of productivity and adaptability to stress conditions. This goes to prove that cultivation of domestic spring wheat varieties will promote formation of high and quality grain yields.

Purpose. To create new competitive spring wheat varieties. Methods. Field study, laboratory test. Results. Based on the competitive variety trial, bread spring wheat line ‘Lutescens 07-26’ has been selected due to high values of such traits as resistance to fungal diseases, grain qua­lity(protein content accounted for 15.0%), 1000 kernel weight (44.6 g) productivity (3.92 t/ha) and lodging resistance (9 points). In 2011, it was submitted to the State variety testing as ‘Panianka’ variety. Durum spring wheat line ‘Leukurum 08-11’ was characterized by a number of positive traits: quite a high productivity (3.05 t/ha), short stem (79 cm), resistance to fungal diseases and lodging(9 points), and in 2011 it was submitted to the State variety testing as ‘Diana’ variety. According to the results of the State variety testing in 2012–2014, spring wheat varieties ‘Panianka’ and ‘Diana’ in 2015 were put on the State Register of plant varieties suitable for dissemination in Ukraine. Conclusions. For farms in Forest-Steppe and Polissia zones of Ukraine, bread and durum spring wheat varieties were bred by V. M.Remeslo Myronivka Institute of Wheat of NAAS of Ukraine that demonstrated rather high potential of productivity and adaptability to stress conditions. This goes to prove that cultivation of domestic spring wheat varieties will promote formation of high and quality grain yields.

Purpose. Review of the main achievements of the Wheat Breeding and Seed ProductionDepartment in the Plant Breeding and Genetic Institute – National Centre of Seed and Cultivar Investigation in the developing theoretical principles of breeding and creation of winter wheat varieties of different types during 100-year (1916–2016) period of breeding programs realization. Results. The main theoretical, methodical developments and breeding achievements of Wheat Breeding and Seed Production Department during 100-year (1916–2016) history have been considered. In the course of the Department activity, the research and metho­dology grounds of bread winter wheat breeding and seed production have been laid, 9 stages of breeding programs development have been accomplished. As a result, more than 130 varieties of different types have been created, 87 of them have been released in some periods or registered in the State registers of plants varieties of Ukraine and other countries and grown in the total sowing area about 220 million hectares.

Мета. Визначення генетичних ефектів ПЖТ 1AL.1RS і 1ВL.1RS на врожайність, елементи продуктивності рослин та показники якості рекомбінантних ліній, установлення ефективності використання кожної з ПЖТ для створення досконаліших за цими ознаками сортів пшениці м’якої озимої в умовах ґрунтово-повітряних посух у степовій зоні України та розроблення селекційних заходівів зменшення негативних ефектів транслокацій для отримання генотипів з високими показниками якості зерна цінної і сильної пшениці. Методи. Польові експерименти, внутрішньовидова гібридизація, оцінювання селекційного матеріалу в польових умовах, методи лабораторного визначення показників хлібопекарських якостей зерна, електрофорез запасних білків, статистичні. Результати. У посушливих умовах Півдня України на великому експериментальному матеріалі селекційного процесу виявлено позитивний вплив ПЖТ 1AL.1RS на врожайність рекомбінантних ліній та основні елементи продуктивності рослин, що проявляється на фоні одночасного позитивного ефекту цієї транслокації на посухо- й жаростійкість. Використання в селекції пшениці ПЖТ 1ВL.1RS у цьому регіоні є менш перспективним заходом. Установлено, що введення шляхом гібридизації в місцевий генофонд пшениці м’якої озимої пшенично-житніх транслокацій 1АL.1RS та 1ВL.1RS змінює показники якості зерна. Зокрема, вміст білка, зазвичай, має тенденцію до підвищення, при цьому він суттєвіше зростає завдяки транслокації 1ВL.1RS. Показано, що частота отримання рекомбінантних ліній, які поєднують високу врожайність та мають добрі хлібопекарські властивості не нижче цінних і сильних пшениць, досить низька (1,7–6,1%). Однак, переваги за цим показником мають інтрогресивні лінії з ПЖТ 1АL.1RS. Використовуючи комбінування в процесі гібридизації ПЖТ з алелями з високим позитивним впливом на хлібопекарські властивості, а також створюючи гетерогенність у складі генотипів з ПЖТ і без них, можна спрямовано зменшувати негативний вплив ПЖТ на якість зерна пшениці м’якої озимої і створювати сорти з параметрами якості цінних і сильних пшениць. Висновки. Отримані результати дають підстави стверджувати, що використання ПЖТ 1AL.1RS є перспективним напрямом подальшого селекційного нарощування генетичного потенціалу врожайності сортів пшениці м’якої озимої в посушливих умовах Півдня України. У результаті повного циклу селекційного процесу на матеріалі з ПЖТ 1АL.1RS, створена серія сортів пшениці м’якої озимої – ‘Житниця одеська’, ‘Октава одеська’, ‘Ліга одеська’, ‘Дума одеська’, ‘Версія одеська’, які забезпечують підвищення врожайності на 10–15% порівняно зі стандартами та занесені до Державних реєстрів сортів рослин України та Молдови. | Purpose. To determine the genetic effects of WRT 1AL.1RS and 1BL.1RS on the yield, plant productivity elements and quality indices of recombinant lines, to determine the effectiveness of using each of the WRT for creating more perfect varieties of soft winter wheat in these traits under soil-air drought in the steppe zone of Ukraine and development of breeding techniques to reduce the negative effects of translocation to produce genotypes with high quality indices of valuable and strong wheat grain. Methods. Field experiments, intraspecific hybridization, evaluation of breeding material in the field, methods of laboratory determination of baking quality indices of grain, electrophoresis of spare proteins, statistical. Results. Under arid conditions of the South of Ukraine on the large experimental material of breeding process, a positive effect of 1AL.1RS on the yield of recombinant lines and the main elements of plant productivity were determined, which was manifested against the background of simultaneous positive effect of this transposition on the drought and heat tolerance. The use of 1BL.1RS in wheat breeding in this region is less promising technique. It has been determined that introduction of 1AL.1RS, 1BL.1RS translocations into local gene pool of soft winter wheat by hybridization changes the grain quality indices. In particular, the protein content tends to increase more significantly under the influence of 1BL.1RS translocation. It has been shown that the frequency of obtaining recombinant lines which combine high yield and sufficient level of baking properties (not lower than valuable and strong wheat) is quite low (1,7–6,1%), but introgressive lines with 1AL.1RS have the advantages in this parameter. Using such genetic factors as hybridization combining WRT with alleles with high positive effect on baking properties, and also creating heterogeneity in the composition of genotypes with and without WRT, one can purposefully reduce the negative impact of WRT on the quality of soft winter wheat grain and create varieties with quality parameters of valuable and strong wheat. Conclusions. In general, the results achieved give reason to assert that the use of WRT 1AL.1RS is a promising direction for further breeding increase of genetic capacity of soft winter wheat varieties in the arid conditions of the South of Ukraine. As a result of full cycle of breeding process on the material with 1AL.1RS WRT a series of varieties of soft winter wheat ‘Zhytnytsia odeska’, ‘Oktava odeska’, ‘Liha odeska’, ‘Duma odeska’, ‘Versiia odeska’, providing 10 – 15% increase in yield to standards was created and included in the State Register of Ukraine and Moldova.

The morphological and agrobiological characteristics and qualities, the potential of the new registered winter wheat varieties, which are suitable for intensive technologies have been learned.

Мета. Визначення генетичних ефектів ПЖТ 1AL.1RS і 1ВL.1RS на врожайність, елементи продуктивності рослин та показники якості рекомбінантних ліній, установлення ефективності використання кожної з ПЖТ для створення досконаліших за цими ознаками сортів пшениці м’якої озимої в умовах ґрунтово-повітряних посух у степовій зоні України та розроблення селекційних заходівів зменшення негативних ефектів транслокацій для отримання генотипів з високими показниками якості зерна цінної і сильної пшениці. Методи. Польові експерименти, внутрішньовидова гібридизація, оцінювання селекційного матеріалу в польових умовах, методи лабораторного визначення показників хлібопекарських якостей зерна, електрофорез запасних білків, статистичні. Результати. У посушливих умовах Півдня України на великому експериментальному матеріалі селекційного процесу виявлено позитивний вплив ПЖТ 1AL.1RS на врожайність рекомбінантних ліній та основні елементи продуктивності рослин, що проявляється на фоні одночасного позитивного ефекту цієї транслокації на посухо- й жаростійкість. Використання в селекції пшениці ПЖТ 1ВL.1RS у цьому регіоні є менш перспективним заходом. Установлено, що введення шляхом гібридизації в місцевий генофонд пшениці м’якої озимої пшенично-житніх транслокацій 1АL.1RS та 1ВL.1RS змінює показники якості зерна. Зокрема, вміст білка, зазвичай, має тенденцію до підвищення, при цьому він суттєвіше зростає завдяки транслокації 1ВL.1RS. Показано, що частота отримання рекомбінантних ліній, які поєднують високу врожайність та мають добрі хлібопекарські властивості не нижче цінних і сильних пшениць, досить низька (1,7–6,1%). Однак, переваги за цим показником мають інтрогресивні лінії з ПЖТ 1АL.1RS. Використовуючи комбінування в процесі гібридизації ПЖТ з алелями з високим позитивним впливом на хлібопекарські властивості, а також створюючи гетерогенність у складі генотипів з ПЖТ і без них, можна спрямовано зменшувати негативний вплив ПЖТ на якість зерна пшениці м’якої озимої і створювати сорти з параметрами якості цінних і сильних пшениць. Висновки. Отримані результати дають підстави стверджувати, що використання ПЖТ 1AL.1RS є перспективним напрямом подальшого селекційного нарощування генетичного потенціалу врожайності сортів пшениці м’якої озимої в посушливих умовах Півдня України. У результаті повного циклу селекційного процесу на матеріалі з ПЖТ 1АL.1RS, створена серія сортів пшениці м’якої озимої – ‘Житниця одеська’, ‘Октава одеська’, ‘Ліга одеська’, ‘Дума одеська’, ‘Версія одеська’, які забезпечують підвищення врожайності на 10–15% порівняно зі стандартами та занесені до Державних реєстрів сортів рослин України та Молдови.

The new rules formulated in Article 59 of the International Code of Nomenclature for algae, fungi, and plants (ICN) will cause numerous, often undesirable, name changes, when only phylogenetically defined clades are named. Our task is to name fungal taxa and not just clades. Two suggestions are made here that may help to alleviate some disadvantages of the new system. (1) Officially an epithet coined in a list-demoted genus that is older than the oldest one available in the list-accepted genus would have to be recombined in the accepted genus. We recommend that individual authors and committees establishing lists of protected names should generally not recombine older epithets from a demoted genus into the accepted genus, when another one from pre-2013 is available in that genus. (2) Because the concepts of correlated teleomorph and anamorph genera are often incongruent, enforced congruence leads to a loss of information. Retaining the most suitable generic name is imperative, even when this is subordinated to another, list-accepted, generic name. Some kind of cryptic dual generic nomenclature is bound to persist. We therefore strongly recommend the retention of binomials in genera where they are most informative. With these recommendations, the upheaval of fungal nomenclature ensuing from the loss of the former Art. 59 can be reduced to an unavoidable minimum.

Purpose. Review of the main achievements of the Wheat Breeding and Seed ProductionDepartment in the Plant Breeding and Genetic Institute – National Centre of Seed and Cultivar Investigation in the developing theoretical principles of breeding and creation of winter wheat varieties of different types during 100-year (1916–2016) period of breeding programs realization. Results. The main theoretical, methodical developments and breeding achievements of Wheat Breeding and Seed Production Department during 100-year (1916–2016) history have been considered. In the course of the Department activity, the research and metho­dology grounds of bread winter wheat breeding and seed production have been laid, 9 stages of breeding programs development have been accomplished. As a result, more than 130 varieties of different types have been created, 87 of them have been released in some periods or registered in the State registers of plants varieties of Ukraine and other countries and grown in the total sowing area about 220 million hectares.

Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, pri­mers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are pre­sent only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.

Purpose. Investigation of maize ae1 gene polymorphism by bioinformatic methods. Methods. Global and local alignment of the nucleotide and amino acid sequences, in silico translation and transcription, translates modeling, pri­mers design, phylogenetic analysis. Results. 255 nucleotide sequences of maize аe1 gene, 500 amino acid sequences of homology translates of maize ae1 gene (SBEIIb enzyme homologs) and 100 mRNA expressed from the maize ae1 gene were analyzed to establish phylogenetic relationships. Polymorphism of maize ae1 gene different regions was investigated by bioinformatic methods. Modeling of the maize enzyme SBEIIb was performed. Conclusions. According to the results of amino acid sequences of SBEIIb enzyme homologs alignment, it was found that ae1 gene orthologs are pre­sent only in monocots, paralogs – in monocots, dicots, and other taxa, including algae and animals. Based on the results of alignment of plants mRNA from which enzyme SBEIIb is translated, maize ae1 gene orthologs and the nearest paralogs encoding starch branching enzymes with chloroplast localization were defined; this suggests a possible origin of ae1 gene due to duplication of the gene encoding the 1,4-alpha-glucan-branching enzyme 2 with chloroplast or amyloplast localization. In the maize ae1 gene structure, regions were found that include polymorphic sites not defined previously. For the polymorphic sites design primers were developed that allowed to differentiate the maize lines. It was determined that the detection of polymorphism in theory can influence the enzyme function and, as a result, change the concentration of amylopectin in maize grain.

Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory stu­dies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat varie­ty ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integ­rated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limi­ted application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain qua­lity of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integra­ted crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilize­rs (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.

Purpose. To develop and improve adaptive technologies of winter wheat cultivation which provide high productivity and quality of grain. Methods. Field and laboratory stu­dies, mathematical and statistical analysis. Results. During 2011–2015, the impact of cultivation technologies on the formation of grain quality and yield of winter wheat varie­ty ‘Stolychna’ (with pea as predecessor) was studied. When using alternative technologies with only predecessor by-products application, the yield of winter wheat with integ­rated crop protection system was 4,56 t/ha, with minimal protection – 4.25 t/ha with grain quality of the 5th class of B group. Resource saving cultivation technologies with limi­ted application of fertilizers (Р45К45N30(II)+30(IV)) provided productivity at the level of 4,87–5,50 t/ha with grain qua­lity of the 2nd–3rd class of A group. Grain yield of 6.01 t/ha with indicators of the 2nd–3rd class of quality was obtained with the use of intensive cultivation technology with application of mineral fertilizers (Р90К90N30(II)+60(IV)+30(VIII)) on the background of applying predecessor’s by-products and integra­ted crop protection. The highest yield of grain (6.22 t/ha) with indicators of the 2nd class of A group quality on average for the research period was provided by energy-intensive technology, that requires the application of mineral fertilize­rs (P135K135N60(II)+75(IV)+45(VIII)) and incorporation of predecessor’s by-products in the soil, and integrated plant protection. Conclusion. It was found that in the northern part of the Right-Bank Forest-Steppe zone of Ukraine, the highest productivity of winter wheat was obtained in dark grey podzolic soils when using the energy-intensive technology with application of P135K135N60(II)+75(IV)+45(VIII) on the background of predecessor’s by-products and integrated crop protection. This technology ensured the grain yield of 6.22 t/ha of the 2nd class of A group quality.

Purpose. To develop a conceptually new method for determination of varietal purity (typicality), hybridity, steri­lity of seed lots. Methods of molecular biology (genomic DNA extraction, PCR with SSR markers application, capillary electrophoresis), genetic, statistical, mathematical analysis. Results. New method for determining the varietal qualities of seed lot was developed that consists of the following steps: simultaneous DNA extraction from a representative sample of aggregated seeds; PCR and further analysis of the amplification products by determination of the qualitative and quantitative composition of SSR-markers’ alleles; calculation of values of varietal seed lot quality using experimentally derived allele ratios. Conclusions. The developed method for determining varietal qualities of seed lots allows to reduce significantly the consumption of materials, time and labor during the analysis. Consistent qualification and quantification of alleles in the total sample of a seed lot is a conceptually new approach to establish varietal purity (typicality), hybridity, sterility.

ß-tubulin, calmodulin, internal transcribed spacer and partial Isu-rDNA, RNA polymerase 2, DNA replication licensing factor Mcm7, and pre-rRNA processing protein Tsr1 were amplified and sequenced from numerous isolates belonging to Aspergillus sect, versicolor. The isolates were analyzed phylogenetically using the concordance model to establish species boundaries. Aspergillus austroafricanus, A. creber, A. cvjetkovicii, A. fructus, A. jensenii, A. puulaauensis, A. subversicolor, A. tennesseensis and A. venenatus are described as new species and A. amoenus, A. protuberus, A. sydowii, A. tabacinus and A. versicolor are accepted as distinct species on the basis of molecular and phenotypic differences. PCR primer pairs used to detect A. versicolor in sick building syndrome studies have a positive reaction for all of the newly described species except A. subversicolor.

Purpose. Theoretical and experimental substantiation of introduction of “Romantica” roses originated by Meilland company under the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. Methods. Biometrical, comparative-morphological, statistic ones. Results. Viability, growth and development indices as well as ornamental qualities (tolerance for winter conditions, frost hardiness, drought resistance, complex disease and pest resistance, habit maintenance, shoot formation, flowering, coloration, shape of flowers, petal number etc.) were analyzed for 12 rose cultivars of “Romantica” series planted on the collection and exhibition plots of the National Dendrological Park «Sofiivka» NAS of Ukraine. The investigations were conducted within 2012–2015. The most of the cultivars got the mark of introduction success 41–45 points of 50; so, the cultivars are totally promising for introduction into the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. ‘Leonardo da Vinci’ and ‘Yves Piaget’ cultivars got point number 35–38 and they can be considered promising ones. The acclimatization number for the most of the investigated cultivars was 85–90 points of 100 that means good adaptation of the plants in the conditions of the Right-Bank Forest-Steppe Zone of Ukraine. The adaptation of cv. ‘Leonardo da Vinci’ and cv. ‘Yves Piaget’ roses (point number 75) can be considered satisfactory. The total evaluation of “Romantica” roses ornamental qualities made 76–87 points of 100; so, all the cultivars display high ornamental qualities and deserve to be introduced to the production. Conclusions. The garden roses of “Romantica” series originated by Meilland company being introduced into the conditions of the Right-Bank Forest-Steppe Zone of Ukraine are adapted well in the introduction region and they are promising for the subsequent introduction.

The study of impact of complex application of biological compositions based on fixing nitrogen by microorganisms and plant lectin and nitric fertilizers on productivity and quality of grain of winter wheat on the dark-grey soil in the field terms was conducted. It is shown, that presowing treatment of seed by biological compositions stimulated forming of greater productivity with the best quality.

Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tole­rance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-to­lerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.

Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tole­rance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-to­lerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.

Purpose. To establish the level of formation of winter barley yield depending on weather conditions during the gro­wing season in the Forest-Steppe zone of Ukraine. Methods. Field investigations, ANOVA, variance and correlation analysis. Results. Positive correlation was revealed between yield and the duration of the vegetation (r = 0.54) and especially “heading–maturation” period (r = 0.76). Moderate positive correlation was established between yield and mean daily air temperature during the period from termination to resumption of vegetation (r = 0.44), and strong negative one – between yield and mean daily air temperature during hea­ding to maturation period (r = -0.77). Positive correlation was observed between yield and rainfall amount during the period from vegetation resumption to heading (r = 0.30). In general, during the growing season the correlation was weak (r = 0.16). For the period of “heading–maturation” low nega­tive value (r = -0.11) was fixed. This was due to heavy precipitations, especially torrential rains, which in some years have caused lodging of plantings resulted in yield decrease. Conclusions. Under the conditions of Forest-Steppe zone of Ukraine, fluctuations in temperature and water regimes are responsible for significant variability in growing season duration and yield of winter barley. Optimal amount of precipitation (close to long-time annual average) and their uniform distribution throughout growing season is the most favorable for obtaining high yield. “Heading–maturation» period is responsible for the formation of winter barley yield level.

Purpose. To establish the level of formation of winter barley yield depending on weather conditions during the gro­wing season in the Forest-Steppe zone of Ukraine. Methods. Field investigations, ANOVA, variance and correlation analysis. Results. Positive correlation was revealed between yield and the duration of the vegetation (r = 0.54) and especially “heading–maturation” period (r = 0.76). Moderate positive correlation was established between yield and mean daily air temperature during the period from termination to resumption of vegetation (r = 0.44), and strong negative one – between yield and mean daily air temperature during hea­ding to maturation period (r = -0.77). Positive correlation was observed between yield and rainfall amount during the period from vegetation resumption to heading (r = 0.30). In general, during the growing season the correlation was weak (r = 0.16). For the period of “heading–maturation” low nega­tive value (r = -0.11) was fixed. This was due to heavy precipitations, especially torrential rains, which in some years have caused lodging of plantings resulted in yield decrease. Conclusions. Under the conditions of Forest-Steppe zone of Ukraine, fluctuations in temperature and water regimes are responsible for significant variability in growing season duration and yield of winter barley. Optimal amount of precipitation (close to long-time annual average) and their uniform distribution throughout growing season is the most favorable for obtaining high yield. “Heading–maturation» period is responsible for the formation of winter barley yield level.

Purpose. Improvement of a range of strawberry varie­ties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of primary variety studying and state variety testing at three-year strawberry plantations of the Institute of Horticulture of the National Academy of Sciences of Ukraine (the city of Kyiv) and its variety testing stations. Results. It was found that more frequent spring and summer droughts as well as black frosts in winter adversely affected the strawberry productivity. The best varieties were selected among new ones for the complex of traits, they included ‘Elegance’ from the Great Britain, ‘Gercules’ from Ukraine (both are dessert grapes), ‘Elsanta’ from Holland (for universal use), ‘Alba’ from Italia (as raw material). The highly adaptive and productive ‘Istochnik’ and ‘Festyvalna romashka’ varieties (as raw material) are recommended for the north regions. Conclusions. In the context of profound climatic change, high-profitable cultivation of mid-ripening varieties and a strawberry as such in the Northern Forest-Steppe zone is expedient under controlled conditions – in high film tunnels, in hot-houses and under perennial irrigation that already has become the European tendency.

Purpose. Improvement of a range of strawberry varie­ties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of primary variety studying and state variety testing at three-year strawberry plantations of the Institute of Horticulture of the National Academy of Sciences of Ukraine (the city of Kyiv) and its variety testing stations. Results. It was found that more frequent spring and summer droughts as well as black frosts in winter adversely affected the strawberry productivity. The best varieties were selected among new ones for the complex of traits, they included ‘Elegance’ from the Great Britain, ‘Gercules’ from Ukraine (both are dessert grapes), ‘Elsanta’ from Holland (for universal use), ‘Alba’ from Italia (as raw material). The highly adaptive and productive ‘Istochnik’ and ‘Festyvalna romashka’ varieties (as raw material) are recommended for the north regions. Conclusions. In the context of profound climatic change, high-profitable cultivation of mid-ripening varieties and a strawberry as such in the Northern Forest-Steppe zone is expedient under controlled conditions – in high film tunnels, in hot-houses and under perennial irrigation that already has become the European tendency.

Purpose. Assessing stability of yield formation of complex-valued varieties and lines of spring triticale under conditions and of different years of their growing. Identifying the best genotypes for yield potential, plasticity and stability. Methods. Ontogenetic, statistical and mathematical analyses. Results. Adaptive capacity and breeding value of spring triticale varieties and lines was established. Methods of breeding and parentage of the best samples were analyzed. Comparison of yields under conditions in of different years of growing allowed to identify genotypes with high yield potential – ‘ЯТХ 38-14’, ‘ЯТХ 61-14’, ‘ЯТХ 62-14’, with stable yields – ‘ЯТХ 17-14’, ‘Boryviter kharkivskyi’, ‘ЯТХ 43-14’, ‘Gusar kharkivskyi’, drought resistance ones – ‘ЯТХ 37-14’, ‘ЯТХ 43-14’, ‘ЯТХ-64-14’. The genotypes ‘Lebіd kharkіvskyi’, ‘ЯТХ 38-14’, ‘ЯТХ 62-14’ appeared to be more plastic. These samples may be used of hybridization to improve yield. Conclusions. The most promising to production and breeding are the lines ‘ЯТХ 17-14’, ‘ЯТХ 64-14’, ‘ЯТХ 37-14’, ‘ЯТХ 43-14’ (their average yield 4,27–4,48 t/ha) and varieties ‘Zlit kharkivskyi’, ‘Boryviter kharkivskyi’, ‘Gusar kharkivskyi’, ‘Darkhliba kharkivskyi’, which have high adaptive capacity combined with high productivity. They are valuable for use as a starting material in breeding for adaptability.

Мета. Встановити сортові особливості формування продуктивності гречки (Fagopyrum esculentum Moench) в умовах нестійкого зволоження Центрального Лісостепу залежно від строків та способів сівби. Методи. Упродовж 2015–2017 рр. в умовах Устимівської дослідної станції рослинництва Інституту рослинництва імені В. Я. Юр’єва НААН досліджували 6 сортів гречки: ‘Ярославна’, ‘Слобожанка’, ‘Руслана’, ‘Українка’, ‘Крупинка’, ‘СИН-3/02’. Висівали гречку нормою 250 зерен/м2 широкорядним (міжряддя – 45 см) та звичайним рядковим (15 см) способом у три строки з інтервалом у 5 діб: 5–6 травня (температура ґрунту 14,5–16,6 °С), 10–11 та 15–16 травня (16,4–19,1 та 16,9–17,3 °С відповідно).Результати. Результати трирічного експерименту засвідчують істотний вплив досліджуваних факторів (сорту, строку і способу сівби) на формування елементів продуктивності гречки, що змінювалися в досить широкому діапазоні (кількість зерен з однієї рослини – від 2,8 до 17,4 шт., маса 1000 зерен – до 2,4 г, урожайність – від 0,1 до 1,2 т/га) і значною мірою залежали від погодних умов весняного періоду. Посушливі умови на початку вегетації знижували крупність зерна та озерненість рослин, що зумовлювало зменшення врожайності, тоді як більш вологі умови навпаки збільшували ці показники. Водночас, сорти ‘СИН-3/02’ і ‘Крупинка’ в посушливих умовах 2017 року відреагували збільшеням врожайності в разі сівби звичайним рядковим способом. Якщо в попередні роки вони забезпечували вищу врожайність за широкорядного висіву, то в несприятливих для росту й розвитку рослин умовах, доцільнішим виявився звичайний рядковий спосіб, а строк сівби істотного впливу на цей показник не мав. Строками сівби гречки (з різницею в 5 діб) не завжди можна нівелювати негативний вплив погодних умов. Спосіб сівби є дієвим фактором впливу на формування високих показників структурних елементів продуктивності та потенціалу врожайності зерна гречки, що певною мірою обумовлено біологічними особливостями сорту.Висновки. Серед досліджуваних сортів гречки найбільшою врожайністю відзначався сорт ‘Ярославна’ за сівби широкорядним способом у першій декаді травня (5–6.05). Ці елементи технології вирощування були кращими і для сортів ‘Слобожанка’ і ‘Руслана’. Другий строк сівби (10–11 травня) є сприятливішим для сортів ‘СИН-3/02’ та ‘Українка’ за широкорядного способу сівби. Гречка ‘Крупинка’ забезпечувала найбільшу продуктивність за сівби в першій-другій декаді травня широкорядним способом. У разі сівби в третій строк (15–16.05) усі сорти формували зменшені показники елементів продуктивності порівняно з іншими строками і способами сівби. | Purpose. To study the varietal peculiarities of the buckwheat (Fagopyrum esculentum Moench) productivity formation in the conditions of unstable hydration of the Central Forest-Steppe zone of Ukraine, depending on the timing and methods of sowing.Methods. During 2015–2017, at the Ustymivka Experimental Station of Plant Production (Yuriev Plant Production Institute, NAAS of Ukraine) 6 varieties of buckwheat: ‘Yaroslavna’, ‘Slobozhanka’, ‘Ruslana’, ‘Ukrainka’, ‘Krupynka’, ‘SIN 3/02’ were studied. Buckwheat was sowed at a rate of 250 grains/m2 by wide (row spacing – 45 cm) and usual row (15 cm) methods into 3 steps with 5 days intervals: 5–6 May (soil temperature 14.5–16.6 °C), 10–11 and 15–16 May (16.4–19.1 and 16.9–17.3 °C correspondently).Results. The results of the three-year experiment indicate a significant impact of the factors (variety, timing and method of sowing) on the buckwheat productivity formation, which varied in a rather wide range: the number of grains on one plant varied from 2.8 to 17.4 pcs.; the weight of 1000 grains – up to 2.4 g; yield – from 0.1 to 1.2 t/ha; and in higher degree depended on the weather conditions in the spring period. The arid conditions at the beginning of the vegetation phase reduced the grain size and number on the plants that caused a decrease in yield, while more humid conditions increased these rates. At the same time, the varie­ties ‘SIN-3/02’ and ‘Krupynka’ in the dry conditions of 2017 showed increased yields in the case of sowing in the usual routine manner. In previous years they provided higher yields for wide-row sowing, while in unfavorable conditions for growth and development, the usual routine method was more suitable, and the term of sowing did not have a significant impact on this. The terms of sowing with the intervals in 5 days, can not mitigate the negative effects of weather conditions, in general. The method of sowing is an effective factor for high buckwheat productivity and yield formation that to a certain degree depends on biological characteristics of the variety. Conclusions. Among the studied buckwheat varieties, the ‘Yaroslavna’ variety characterized by the highest yield in case of wide-row sowing in the first decade of May (5–6.05). These elements of cultivation technology were better for the varieties ‘Slobozhanka’ and ‘Ruslana’. The second term of sowing (May 10–11) is more favorable for the varieties ‘SIN-3/02’ and ‘Ukrainka’ with the wide-row sowing. Buckwheat ‘Krupynka’ provided the highest yield with wide-row sowing in the first or second decade of May. In the case of sowing in the third term (15–16.05) all the varieties characterized by reduced productivity in comparison with other terms and methods of sowing | Цель. Установить сортовые особенности формирования продуктивности гречихи (Fagopyrum esculentum Moench) в условиях неустойчивого увлажнения Центральной Лесостепи Украины в зависимости от сроков и способов сева. Методы. В течение 2015–2017 гг. в условиях Устимовской опытной станции растениеводства Института растениеводства имени В. Я. Юрьева НААН исследовали 6 сортов гречихи: ‘Ярославна’, ‘Слобожанка’, ‘Руслана’, ‘Українка’, ‘Крупинка’, ‘СИН-3/02’. Высевали гречиху нормой 250 зерен/м2 широкорядным (междурядья – 45 см) и обычным строчным (15 см) способом в три срока с интервалом в 5 суток: 5–6 мая (температура почвы 14,5–16,6 °С), 10–11 и 15–16 мая (16,4–19,1 и 16,9–17,3 °С соответственно). Результаты. Результаты трехлетнего эксперимента свидетельствуют о существенном влиянии исследуемых факторов (сорта, срока и способа сева) на формирование элементов продуктивности гречихи, что изменялись в достаточно широком диапазоне (количество зерен с одного растения – от 2,8 до 17,4 шт., масса 1000 зерен – до 2,4 г, урожайность – от 0,1 до 1,2 т/га) и в значительной степени зависели от погодных условий весеннего периода. Засушливые условия в начале вегетации снижали крупность зерна и озерненность растений, что приводило к уменьшению урожайности, тогда как более влажные условия наоборот увеличивали эти показатели. В то же время, сорта ‘СИН-3/02’ и ‘Крупинка’ в засушливых условиях 2017 года отреагировали увеличением урожайности в случае сева обычным строчным способом. Если в предыдущие годы они обеспечивали высокую урожайность при широкорядном посеве, то в неблагоприятных для роста и развития растений условиях более целесообразным оказался обычный строчный способ, а срок сева существенного влияния на этот показатель не имел. Сроками сева гречихи (с разницей в 5 дней) не всегда можно нивелировать негативное влияние погодных условий. Способ сева является действенным фактором влияния на формирование высоких показателей структурных элементов продуктивности и потенциала урожайности зерна гречихи, что в определенной степени обусловлено биологическими особенностями сорта. Выводы. Среди изучаемых сортов гречихи наибольшей урожайностью отличался сорт ‘Ярославна’ при посеве широкорядным способом в первой декаде мая (5–6.05). Эти элементы технологии выращивания были лучшими также для сортов ‘Слобожанка’ и ‘Руслана’. Второй срок сева (10–11 мая) является благоприятным для сортов ‘СИН-3/02’ и ‘Українка’ при широкорядном способе посева. Гречиха ‘Крупинка’ обеспечивала наибольшую продуктивность при посеве в первой-второй декаде мая широкорядным способом. В случае сева в третий срок (15–16.05) все сорта формировали уменьшенные показатели элементов продуктивности по сравнению с другими сроками и способами посева.