The article highlights issues in establishing and maintaining variety collections that display example characteristics of Sorghum Bicolor. It also displays topicality and value of those varieties. Varietal composition of common knowledge (30 items) variety collection of Sorghum bicolor has been scrutinized, which served as a baseline for establishing collection of varieties exhibiting example characteristics (16 items).

Purpose. To enrich and improve the range of table grape varieties of Ukraine. Methods. Breeding, hybridological, mathematic, visual ones. Results. Modern Ukrainian gene pool of table grape varieties has been created on the base of the best introduced and homegrown cultivars and selections. New genotypes that are creating at the National Scientific Centre “Institute of Viticulture and Wine-Making named after V. Ye. Tairov” under the breeding program “Resistense plus Quality” are the basis of current and future regional assortments for the south and central areas of the steppe zone of Ukraine. The complexity of the origine explains the genetically determined high-level manifestation of commercially valuable characters. New varieties and selections are ecologically safe for humans and the environment, as the level of their resistance to the group of fungal main diseases is not less than 6.5 points according to the 9-point scale, therefore they do not require a large pesticide load. New promising table varieties demonstrate a stable yield and high marketability of grapes – 5–10 kg per plant with the marketability nearly 80–87% during the last five years. In addition, dense flesh of grapes is a guarantee of high transportability. The variety of table genotypes taste, shape and berries ripening from very early to very late embodied in the innovative and highly profitable development “Conveyor of table grapes”. Depending on the ecological and geographical conditions, the conveyor can include a diffe­rent quantity of varieties, but to in order increase the efficiency and profitability it is necessary to have a few groups (from 2 to 4 varietes) of different ripening. The approximate ratio (%) of varieties of different ripening time is recommended: very early – 22%, early – 22%, average – 17%, mid-late – 17%, and late – 22%. Conclusions. The variation of shape, flavour and color of new promising genotypes of table grapes makes them very popular on the market and may satisfy the most demanding consumer. The stability of the adaptive and technological traits makes the new table varieties very attractive for the producers of grape products.

The article offers results of determination of labor input and predicted costs for qualifying examination of distinctness, uniformity and stability for varieties of the legumes. That will facilitate cost optimization and enable planning expenditures on scientific and planning basis.

True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have simple body plans with often morphologically and ecologically obscure structures. This poses challenges for accurate and precise identifications. Here we provide a conceptual framework for the identification of fungi, encouraging the approach of integrative (polyphasic) taxonomy for species delimitation, i.e. the combination of genealogy (phylogeny), phenotype (including autecology), and reproductive biology (when feasible). This allows objective evaluation of diagnostic characters, either phenotypic or molecular or both. Verification of identifications is crucial but often neglected. Because of clade-specific evolutionary histories, there is currently no single tool for the identification of fungi, although DNA barcoding using the internal transcribed spacer (ITS) remains a first diagnosis, particularly in metabarcoding studies. Secondary DNA barcodes are increasingly implemented for groups where ITS does not provide sufficient precision. Issues of pairwise sequence similarity-based identifications and OTU clustering are discussed, and multiple sequence alignment-based phylogenetic approaches with subsequent verification are recommended as more accurate alternatives. In metabarcoding approaches, the trade-off between speed and accuracy and precision of molecular identifications must be carefully considered. Intragenomic variation of the ITS and other barcoding markers should be properly documented, as phylotype diversity is not necessarily a proxy of species richness. Important strategies to improve molecular identification of fungi are: (1) broadly document intraspecific and intragenomic variation of barcoding markers; (2) substantially expand sequence repositories, focusing on undersampled clades and missing taxa; (3) improve curation of sequence labels in primary repositories and substantially increase the number of sequences based on verified material; (4) link sequence data to digital information of voucher specimens including imagery. In parallel, technological improvements to genome sequencing offer promising alternatives to DNA barcoding in the future. Despite the prevalence of DNA-based fungal taxonomy, phenotype-based approaches remain an important strategy to catalog the global diversity of fungi and establish initial species hypotheses.

True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have simple body plans with often morphologically and ecologically obscure structures. This poses challenges for accurate and precise identifications. Here we provide a conceptual framework for the identification of fungi, encouraging the approach of integrative (polyphasic) taxonomy for species delimitation, i.e. the combination of genealogy (phylogeny), phenotype (including autecology), and reproductive biology (when feasible). This allows objective evaluation of diagnostic characters, either phenotypic or molecular or both. Verification of identifications is crucial but often neglected. Because of clade-specific evolutionary histories, there is currently no single tool for the identification of fungi, although DNA barcoding using the internal transcribed spacer (ITS) remains a first diagnosis, particularly in metabarcoding studies. Secondary DNA barcodes are increasingly implemented for groups where ITS does not provide sufficient precision. Issues of pairwise sequence similarity-based identifications and OTU clustering are discussed, and multiple sequence alignment-based phylogenetic approaches with subsequent verification are recommended as more accurate alternatives. In metabarcoding approaches, the trade-off between speed and accuracy and precision of molecular identifications must be carefully considered. Intragenomic variation of the ITS and other barcoding markers should be properly documented, as phylotype diversity is not necessarily a proxy of species richness. Important strategies to improve molecular identification of fungi are: (1) broadly document intraspecific and intragenomic variation of barcoding markers; (2) substantially expand sequence repositories, focusing on undersampled clades and missing taxa; (3) improve curation of sequence labels in primary repositories and substantially increase the number of sequences based on verified material; (4) link sequence data to digital information of voucher specimens including imagery. In parallel, technological improvements to genome sequencing offer promising alternatives to DNA barcoding in the future. Despite the prevalence of DNA-based fungal taxonomy, phenotype-based approaches remain an important strategy to catalog the global diversity of fungi and establish initial species hypotheses.

Analyzed has been conducted on parameters of productivity and efficiency increase for Helios and Comandor varieties of spring barley (Hordeum vulgare L.) on fertilized NPK background after soya as a forecrop.

Apiotrichum is a diverse anamorphic basidiomycetous yeast genus, and its mitogenome characterization has not been revealed. In this study, we assembled two Apiotrichum mitogenomes and compared them with mitogenomes from Agaricomycotina, Pucciniomycotina and Ustilaginomycotina. The mitogenomes of Apiotrichum gracile and A. gamsii comprised circular DNA molecules, with sizes of 34,648 bp and 38,096 bp, respectively. Intronic regions were found contributed the most to the size expansion of A. gamsii mitogenome. Comparative mitogenomic analysis revealed that 6.85–38.89% of nucleotides varied between tRNAs shared by the two Apiotrichum mitogenomes. The GC content of all core PCGs in A. gamsii was lower than that of A. gracile, with an average low value of 4.97%. The rps3 gene differentiated the most among Agaricomycotina, Pucciniomycotina and Ustilaginomycotina species, while nad4L gene was the most conserved in evolution. The Ka/Ks values for cob and rps3 genes were > 1, indicating the two genes may be subjected to positive selection in Agaricomycotina, Pucciniomycotina and Ustilaginomycotina. Frequent intron loss/gain events and potential intron transfer events have been detected in evolution of Agaricomycotina, Pucciniomycotina and Ustilaginomycotina. We further detected large-scale gene rearrangements between the 19 mitogenomes from Agaricomycotina, Pucciniomycotina and Ustilaginomycotina, and fifteen of the 17 mitochondrial genes shared by Apiotrichum varied in gene arrangements. Phylogenetic analyses based on maximum likelihood and Bayesian inference methods using a combined mitochondrial gene dataset revealed different taxonomic assignment of two Apiotrichum species, wherein A. gamsii had a more closely relationship with Trichosporon asahii. This study served as the first report on mitogenomes from the genus Apiotrichum, which promotes the understanding of evolution, genomics, and phylogeny of Apiotrichum.

Assessment has been carried out for parental lines and hybrid combinations of forage beet by individual indices of productivity and morphological characteristics of root storages. Best combinations have been selected for further crossing and breeding.

Assessment has been carried out for parental lines and hybrid combinations of forage beet by individual indices of productivity and morphological characteristics of root storages. Best combinations have been selected for further crossing and breeding.